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Citations for Millipore Sigma :
551 - 600 of 7426 citations for rno mir 134 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Neuroscience 2022Quote: Genotyping for the R1.40 transgene locus was done with the recommended PCR primers using the PCR Ready Mix kit (E3004; Sigma-Aldrich). The sequences of the genotyping primers were as follows ...
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bioRxiv - Genetics 2021Quote: ... Purification of genomic Polymerase Chain Reaction (PCR) amplified DNA was performed with the GenElute PCR Clean-Up kit (Sigma-Aldrich) or the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... or LIVE/DEAD™ Fixable Blue Cells Stain Kit (#L34957, Thermo; 1:1000 in PBS) and fixation for 15 minutes at RT using 1.85% formaldehyde (F1635, Sigma) in PBS solution before surface staining with antibodies in PBS supplemented with 0.5% BSA [fraction V ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purified FH-Ago2 bound with miRNA (most population bound with miR-122 due to its overexpression) was eluted from anti-FLAG beads by competing with 3X FLAG peptide (Sigma) as per the manufacturer’s protocol in a purification buffer (30mM HEPES pH 7.4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was purified using the GeneElute PCR Clean-Up Kit (Sigma-Aldrich, USA), then labeled with biotin-16-dUTP (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Mice were genotyped using Extract-N-Amp Tissue PCR Kit (Sigma Aldrich) according to manufacturers’ instructions with the following primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we purified both libraries using the GenElute PCR Clean-Up Kit (Sigma) to remove short library fragments ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Mice were genotyped using Extract-N-Amp Tissue PCR Kit (Sigma Aldrich) according to manufacturer instructions with the following primers ...
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bioRxiv - Physiology 2022Quote: ... and purified with the GenElute™ PCR Clean-Up Kit (SIGMA NA1020). 300 ng of the eluted DNA was digested overnight at 37°C with the restriction enzymes including USP8 – BstBI and USP48 – SpeI ...
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bioRxiv - Neuroscience 2023Quote: ... and PCR amplification was performed using KAPA2G ReadyMix Kit (Sigma Aldrich, KK5103) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: PCRs were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) using the following conditions ...
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bioRxiv - Cell Biology 2022Quote: ... for 1h at RT, blocked for 1h at RT in blocking solution (0.3% Triton X-100, 0.2% BSA (A4503, Sigma), and 5% goat serum (005-000-121 ...
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bioRxiv - Molecular Biology 2020Quote: ... with Duolink Detection Reagent Green (Sigma, DUO92014) or Orange (DUO92007) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and visualized using chemiluminescence detection (Immobilon, Millipore). Coomassie brilliant blue staining was conducted using Bullet CBB Stain One Super (Nacalai ...
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bioRxiv - Cell Biology 2021Quote: ... Protein detection using anti-HA (H9658, Sigma) or anti-Scp160 antibodies (Gift of R ...
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bioRxiv - Cell Biology 2021Quote: ... Detection was performed using TMB T0565 (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... Detection was by chemiluminescence with luminol (Sigma) on BioMaxMR film (Kodak) ...
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bioRxiv - Cancer Biology 2022Quote: ... Enhanced chemiluminescence substrate detection system (Millipore-Sigma) was applied to detect bound antibody complexes and visualized by autoradiography ...
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bioRxiv - Cancer Biology 2022Quote: ... Enhanced chemiluminescence substrate detection system (Millipore-Sigma) was applied to detect bound antibody complexes and visualized by autoradiography ...
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bioRxiv - Molecular Biology 2020Quote: ... and In situ Detection Reagents Orange (Sigma) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... following detection using PNPP substrate (Sigma, Israel).
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bioRxiv - Immunology 2022Quote: ... Staining with AldeRed ALDH detection assay (Sigma) was done following manufacturer’s recommendation with minor modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... and chemiluminescent detection (ThermoFisher Scientific, Millipore WBKLS0500) were used to develop immunoblots ...
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bioRxiv - Cell Biology 2024Quote: ... Detection was examined with chemiluminescent substrate (Millipore) using the manufacturer’s protocols.
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bioRxiv - Immunology 2022Quote: ... and fixed in RT methanol (Sigma-Aldrich) for 2 minutes prior to PAS staining according to manufacturer’s protocol (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... reverse transcribed with AMV RT (Sigma 10109118001), and then cDNA were quantified in multiplex TaqMan qPCR reactions using commercially available probe sets (Thermo Fisher 4453320 ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1U ThermaStop-RT (Sigma-Aldrich) per 50U SSIII to increases RT-PCR specificity ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Immunology 2021Quote: ... we extracted genomic DNA from mouse tails and performed PCR with REDExtract-N-Amp™ Tissue PCR Kit following the manufacturer’s instructions (Sigma-Aldrich) and analyzed samples on agarose gel ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Biochemistry 2021Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as previously described (41,44) ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as described previously (Rumi et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Genotypes were verified by PCR of tail DNA using the RedExtract-N-Amp Tissue PCR Kit (Sigma Aldrich 254-457-8).
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bioRxiv - Cancer Biology 2023Quote: ... Routine genotype analysis of mice was performed by PCR assay on DNA purified from tail biopsies (Extract N-Amp Tissue PCR kit, Sigma Aldrich). Wild-type and KO mutant alleles were distinguished using primers available on request ...
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bioRxiv - Synthetic Biology 2023Quote: ... Real-Time PCR reactions were performed in 20 µL mixtures containing 10 µL KAPA SYBR FAST qPCR Master Mix (2X) (Sigma-Aldrich, KK4605), 200 nM final concentration of species-specific rpoD forward and reverse primer and up to 3 µL of sample template ...
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bioRxiv - Cell Biology 2020Quote: ... Cell death was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit, Millipore-Sigma). Cell proliferation was evaluated by Ki-67 immunostaining (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: PLA was performed on cultured granulosa cells using the Duolink PLA Probes and PLA Fluorescence in situ Detection Kit Red (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: Terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) was performed on whole-mount larvae (ApopTag Rhodamine In situ Apoptosis Detection Kit; Millipore). Embryos were fixed ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... GP:NPC1 interaction was detected by applying the Duolink® In Situ Detection Reagents Red kit following the manufacturer’s instructions (Sigma). After removing excess reagents ...
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bioRxiv - Genetics 2022Quote: ... Cell death analysis was performed using the ApopTag® Fluorescein in situ Apoptosis Detection Kit (Sigma-Aldrich, Cat. No. S7110) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) histochemistry was performed using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore), according to the instructions of the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... The proximity ligation reaction and amplification of signal were performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for mouse and rabbit antibodies (Duolink, Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Developmental Biology 2021Quote: Cell death was assessed using the TdT-mediated dUTP nick end-labeling (TUNEL) method (ApopTagPeroxidase In Situ Apoptosis detection kit, Millipore) as per manufacturers’ instructions ...
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bioRxiv - Physiology 2020Quote: Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) was performed on whole-mount larvae (ApopTag Fluorescein In Situ Apoptosis Detection Kit; Millipore) as previously described (Lambert et al. ...