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Citations for Millipore Sigma :
551 - 600 of 7256 citations for West Nile Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using Bradford protein assay (Sigma, Missouri, USA). The total protein was then resolved on SDS-PAGE (8-12% ...
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bioRxiv - Immunology 2023Quote: ... and immunoprecipitated with protein A and protein G sepharose beads (Millipore Sigma). After thoroughly washing beads with SLBA buffer (0.15M NaCl ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3 ...
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bioRxiv - Plant Biology 2024Quote: ... After determining the protein concentration with Bradford protein assay reagents (Sigma B6916), the protein extract at 5 mg mL-1 was split into two LoBind 1.5 mL tubes and incubated with 100 µM DAPIA or equal volume of DMSO (1% v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-HA rabbit antibody (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-FLAG mouse antibody (Sigma) ...
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bioRxiv - Physiology 2021Quote: ... All incubations with primary antibodies were performed for 16 hours at 4°C. Blots were developed using SuperSignal West Pico (Pierce, Ref. 34580) or Luminata Forte Western HRP substrate (Merck Millipore, Ref. WBLUF0500).
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bioRxiv - Cell Biology 2023Quote: ... TBS-T was used for washing the membranes. Membranes were developed using SuperSignal West Pico (Pierce, Ref. 34580) or Luminata Forte Western HRP substrate (Merck Millipore, Ref. WBLUF0500).
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bioRxiv - Cancer Biology 2023Quote: ... and chemiluminescent HRP substrates (Supersignal West Pico PLUS, Thermo Fisher Scientific; Pierce ECL Plus, Thermo Fisher Scientific; or Immobilon ECL Ultra, Millipore) (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Tau protein ladder (Sigma) was used in some experiments as a control ...
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bioRxiv - Biophysics 2022Quote: ... BLUeye protein ladder (Sigma) was used as a standard ...
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bioRxiv - Biophysics 2022Quote: ... standard proteins BSA (Sigma), Apoferritin (Sigma ...
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bioRxiv - Plant Biology 2022Quote: Protein A-sepharose (Sigma) was washed twice with 5 column volumes of buffer HNTG [20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein disulfide isomerase (Sigma) was used in positive control reactions and albumin (Research Organics ...
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bioRxiv - Immunology 2021Quote: ... plus protein A (Sigma).
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bioRxiv - Evolutionary Biology 2023Quote: ... Protein standard (Sigma: P5369) was used as standard (ranging from 0-0.5mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... ECL protein detection (Millipore) was performed with a Fusion Fx7 chemiluminescent imager ...
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bioRxiv - Biochemistry 2024Quote: ... S protein agarose (Millipore) was added to each sample ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 or PC-3 cells were transduced with virus-containing culture media supplemented with 10 µg/ml Polybrene (Millipore, Cat #TR-1003-G) for 24 hours and replaced with fresh growth media ...
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bioRxiv - Microbiology 2022Quote: ... variant was obtained from the RIVM (Netherlands) through European Virus Archive global and expanded on VeroE6 cells grown in DMEM (Sigma Aldrich, Cat #D6429) supplemented with 4% of FCS (Gibco ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...
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bioRxiv - Microbiology 2023Quote: ... and 100 μL per well virus solution with or without 1μg/mL TPCK-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA, Cat# 4352157) was added per well ...
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bioRxiv - Microbiology 2024Quote: ... ½-log serially diluted influenza viruses in virus growth medium plus trypsin (DMEM, 0.1% BSA, 1 µg/mL TPCK-treated trypsin (Sigma, St. Louis, MO, USA)) were added to confluent monolayers of MDCK-SIAT1 cells (37 ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Biochemistry 2021Quote: ... 30 KDa for T-protein and L-protein and 100 KDa for P-protein) were purchased from Millipore (Billerica, MA, USA). Mut Express II Fast Mutagenesis kit V2 was purchased from Vazyme (Jiangsu ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were sonicated and protein concentration was determined using BCA protein assay (Sigma). 30 μg of total proteins were loaded on 12% SDS–PAGE gel and separated by electrophoresis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... a marker of protein oxidation (protein carbonyls; OxyBlot; s7150; EMD Millipore, Billerica, MA), and a marker of mitochondrial biogenesis (PGC-1α ...
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genomics 2019Quote: Duolink PLA kit was used to detect protein-protein interactions (Sigma Aldrich DUO92101). PLA was performed according to manufacturer’s protocol plus additional negative controls ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Immunology 2020Quote: Total proteins isolated from BMDCs were lysed in CytoBuster protein extraction reagent (Novagen). Protein quantification was performed using NanoDrop One Spectrophotometer ...
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bioRxiv - Cell Biology 2022Quote: ... the other stained for proteins with SYPRO Red Protein Gel Stain (Sigma Aldrich). Both dyes were used according to the manufacturers’ protocols.
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bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
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bioRxiv - Microbiology 2023Quote: ... or with slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose). For GFP-p65 and GFP-COMMD2 the samples were immunoprecipitated as per manufacturer’s instructions with the elution was recovered in either 5x with Laemmli buffer 74 and boiled at 98°C (dry bath ...