Labshake search
Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Serine threonine protein kinase greatwall MASTL Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... The washed beads were resuspended in 25 μl of kinase assay buffer with 100 μM ATP (A7699, Sigma) supplemented with phosphatase inhibitors and incubated for 20 min in an Eppendorf Thermomixer at 30°C at maximal mixing velocity ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were maintained overnight in mTeSR1 medium containing the Rho-associated kinase (ROCK) inhibitor Y-27632 (Sigma-Aldrich) until cells reached 60-70 % of confluency ...
-
bioRxiv - Cell Biology 2022Quote: The enzymatic activity of pyruvate kinase was measured using EC 2.7.1.40 from Sigma (Bergmeyer, H.U. et al., n.d.) as described previously (Lewandowski et al. ...
-
bioRxiv - Biophysics 2020Quote: ... The coupled enzymatic reaction contained ~2 units of pyruvate kinase/lactic dehydrogenase (rabbit muscle isolate mixture, Sigma Aldrich) 50 mM KPi ...
-
bioRxiv - Microbiology 2022Quote: ... 1.1 U.ml−1 lactate dehydrogenase and 0.8 U.ml−1 pyruvate kinase (rabbit muscle, P0294 from Sigma-Aldrich®) and 0.5 mg.ml−1 MtAPSK (all final concentrations ...
-
bioRxiv - Neuroscience 2022Quote: ... we delivered the Iκ-kinase inhibitor [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide (TPCA-1; Sigma-Aldrich CAS 507475-17-4 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were maintained overnight in mTeSR1 medium containing the Rho-associated kinase (ROCK) inhibitor Y-27632 (Sigma Aldrich) until cells reached 60–70% of confluency ...
-
bioRxiv - Physiology 2023Quote: ... USA) fitted onto a 96-well plate and calibrated with a standard curve of purified creatine kinase (Sigma, Oakville ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich, Cat #F1804) or 8 μg anti-U2AF2 antibody (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
-
bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for the detection of protein expression: actin (1:1,000,Sigma, USA), angiogenin (Ang ...
-
bioRxiv - Molecular Biology 2019Quote: ... DNA sonication and immunoprecipitation of the protein/DNA complexes with specific antibodies (anti-Flag M2, Sigma) or without antibodies as a negative control ...
-
bioRxiv - Immunology 2019Quote: ... Primary antibodies used were: rabbit anti-prosurfactant protein C (AB3786, EMD Millipore, Burlington, MA; 1:1000), rabbit anti-keratin 14 (KRT14 ...
-
Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for protein detection: anti-Flag M2 mouse 1/1,000 (F3165, Sigma), anti-myc 9E10 mouse 1/1,000 (sc-40 ...
-
bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
-
bioRxiv - Cancer Biology 2020Quote: ... The pre-cleared protein solution was incubated with 3 μg anti-FLAG antibody (Sigma Aldrich, #F1804) or IgG (Millipore ...
-
bioRxiv - Neuroscience 2021Quote: ... and mouse monoclonal anti-glial fibrillary acidic protein antibody (G3893, Sigma-Aldrich, St Louis, MO, USA). Immunoreactivity to the antigen was visualized using Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Molecular Probes ...
-
bioRxiv - Plant Biology 2021Quote: ... 2 mg of total protein was then incubated with anti-Flag antibody (M2 monoclonal clone, Sigma) for 1 h with gentle rotation at 4°C and then protein-G coated magnetic beads (Thermo Fisher ...
-
bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used were: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (1:400; Sigma) and goat polyclonal anti-ChaT (1:100 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The precleared lysate was then transferred to Protein G Dynabeads coupled with anti-FLAG antibody (Sigma) and the mixture was incubated for 2 hours at 5°C on a roller ...
-
bioRxiv - Immunology 2022Quote: ... coupled to protein A Sepharose magnetic beads (Ademtech 0423) and anti-TAX1BP1 antibody HPA024432 (Sigma-Aldrich) together with protein A Sepharose (GE Healthcare) ...
-
bioRxiv - Genomics 2020Quote: ... except for the following modifications: immunoprecipitation was carried out using monoclonal antibodies against protein A (Sigma); the lysis buffer contained 10mg/ml heparin (sigma H7405) ...
-
bioRxiv - Microbiology 2020Quote: ... The immunoprecipitated antibody/chromatin complex was collected using Protein A/G magnetic beads (Millipore 16-663). The input and ChIP samples were reverse cross-linked overnight at 45°C using 0.2M final concentration of NaCl ...
-
bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
-
bioRxiv - Physiology 2022Quote: ... protein G beads were coupled to the pre-pro-vasopressin antibody or normal rabbit serum (Sigma) at a 1:50 ratio for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... 4 µg of antibodies and 20 µL of Magna Chip Protein A/G magnetic beads (Millipore) were added and samples were incubated overnight on a rotating wheel at 4°C ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins synthesized in this assay were detected using anti-FLAG antibodies (F3165 SIGMA, 1:4000 dilution) and the Anti-mouse IgG ...
-
bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against glial fibrillary acidic protein (GFAP; 1:400; cat. no. Mab360; Millipore, Burlington, MA) and IBA-1 (1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... The immunoprecipitation was performed by incubating the protein lysate with 1ug of GluA1 antibody (Millipore, ab1504) or 1ug HA antibody (Santa Cruz ...
-
Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... Puromycin incorporation into neosynthesized proteins was assessd with Western blotting and an anti-puromycin antibody (Sigma).
-
bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
-
bioRxiv - Biochemistry 2021Quote: ... and protein was detected by Western blot analysis using primary αRb H3K27me3 antibody (Millipore #07-449), secondary antibody (Biorad #170-6515) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation was carried out with β-catenin monoclonal antibody and Protein G magnetic beads (Sigma-Aldrich). For PCR ...
-
bioRxiv - Cell Biology 2022Quote: ... The same membrane was stripped of antibody and total protein was determined with Coomassie (Sigma, B0149) staining ...
-
bioRxiv - Molecular Biology 2023Quote: ... 60ul of protein G Sepharose beads conjugated to M2 Flag antibody (Millipore Sigma Cat. M8823-5ML) was added to each sample ...
-
bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were detected using chemiluminescence from horseradish peroxidase-coupled secondary antibodies Anti-rabbit HRP (Sigma; A0545), 1:15,000.
-
bioRxiv - Bioengineering 2022Quote: ... Protein bound to the wells were detected with a peroxidase conjugated anti-Flag antibody (Sigma A8592) and quantified with QuantaBlu™ Fluorogenic Peroxidase Substrate Kit (Thermo Scientific™ 15169) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 50µL of protein G beads were coated 2ug of anti-AGO2 antibody (Sigma SAB4200085 Lot# 0000089486) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
-
bioRxiv - Microbiology 2023Quote: ... GST and GST-LegK2 proteins were labelled with an anti-GST antibody from rabbit (A7340; Sigma) and detected with an anti-rabbit secondary antibody from goat conjugated with the fluorochrome Alexa Fluor 488 (A11034 ...
-
bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
-
bioRxiv - Molecular Biology 2023Quote: ... The purification of monoclonal antibodies was performed by affinity chromatography using Hitrap Protein G Column (Sigma) starting from 150 ml of hybridoma cell supernatant ...
-
bioRxiv - Neuroscience 2023Quote: ... The seven target proteins and antibodies for the sFANSeq panel included Neuronal Nuclei Antigen (NeuN, Millipore Cat# ABN91 ...
-
bioRxiv - Cell Biology 2023Quote: ... or anti-v5 antibody and 40 µl of Protein G agarose beads slurry (Millipore, 16-266) in the presence of RNase A (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
-
bioRxiv - Systems Biology 2020Quote: ... Cells were stained for bait proteins using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, Cat# F3165; used at 1:2500), Rabbit-anti-Giantin antibody (Abcam ...
-
bioRxiv - Genomics 2021Quote: ... the aliquot of sheared chromatin fragments were incubated with antibody conjugated Protein A Dynabeads using total histone 3- (unmodified H3 antibody, Millipore #05-499; 1:1000) or histone 4- (unmodified H4 antibody ...
-
bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
-
bioRxiv - Molecular Biology 2020Quote: ... Singlecell iAT2s were plated on Transwells at a density of 520,000 live cells/cm2 in 100μl of CK+DCI with 10μM Rho-associated kinase inhibitor (“Y”; Sigma Y-27632). 600μl of CK+DCI+Y was added to the basolateral compartment ...