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Citations for Millipore Sigma :
551 - 600 of 2445 citations for Recombinant Rat Cntfr His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... rat anti-HA (1:100; Sigma, 11867423001), rabbit anti-PSD95 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-DAT (1:500, Millipore, MAB369). After incubation with a primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-SST (1:500; Millipore MAB354), rabbit anti-nNOS (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Rat monoclonal anti-HA (clone: 3F10; Sigma) was used as primary antibody and goat anti-rat polyclonal Alexa Fluor 594 (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... and rat anti-MBP (1:50, Millipore) diluted in 1% BSA/PBS overnight ...
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bioRxiv - Neuroscience 2023Quote: ... and rat anti-MBP (1:50, Millipore). For studying CNS inmate immune cells ...
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bioRxiv - Immunology 2023Quote: ... IgG from rat serum (I4131, Sigma-Aldrich) was used as isotype control ...
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bioRxiv - Genetics 2023Quote: ... rat anti-HA (Sigma 3F10; 1:500), mouse anti-Pros (DSHB MR1A ...
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bioRxiv - Biophysics 2023Quote: ... rat anti-FLAG (Sigma SAB4200071, 1:1000), rabbit anti-GFP (CST 2956 ...
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bioRxiv - Plant Biology 2023Quote: ... FITC-labelled anti-rat antibody (Sigma F1763). Each antibody incubation was followed by washing three times for 10 min each with 1x PBS ...
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bioRxiv - Genomics 2023Quote: ... normal rat IgG2a (EMD Millipore, MABF 1077Z), IGF-1 (R&D systems ...
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bioRxiv - Genomics 2023Quote: ... the rat anti-HA mAb 3F10 (Sigma) was used at a 1:1000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-SST (MAB354, Millipore; 1:250), rabbit anti-cFos (2250S ...
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bioRxiv - Immunology 2024Quote: ... The same amount of rat IgG (Sigma) was used as isotype control ...
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bioRxiv - Neuroscience 2023Quote: ... rat-anti SST (1:300, Millipore MAB354), goat-anti PV (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-MBP (Millipore #MAB386; 1:50), rabbit anti-Iba1 (WAKO #019-19741 ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG (mouse; Sigma-Aldrich or rat; Agilent), HA (rat ...
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bioRxiv - Cell Biology 2024Quote: ... rat α-hemagglutinin (HA, clone 3F10, Sigma); mouse α-tubulin (clone B-5-1-2 ...
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bioRxiv - Bioengineering 2024Quote: ... Rat serum was obtained from Sigma-Aldrich. Heat treatment was for 65° C ...
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bioRxiv - Biochemistry 2022Quote: ... as a His-tag fusion and Ars2206-531 into pRSFduet vector (Novagen) as a Strep-tag fusion ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...
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bioRxiv - Immunology 2022Quote: 30µL/well of rabbit anti-His tag antibody (Sigma Aldrich, catalog #SAB5600227) was adsorbed at a concentration of 0.5 μg/mL on to 384 well microplates (Thermofisher ...
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bioRxiv - Bioengineering 2022Quote: ... Pre-cleared supernatant was mixed with Ni-NTA His-Bind Resin (Novagen) (Pajenda et al. ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of rcCBP2 was performed by HIS-Select HF Nickel Affinity (Sigma) and gel permeation chromatography on Superdex 75 column (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes followed by anti-his antibody (Sigma, Cat. No. 11922416001) for another 10 mins ...
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bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
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bioRxiv - Plant Biology 2024Quote: ... and confirmed by Western blotting using His-tag antibody (Sigma; SAB 1305538). The His-tagged GUN1-PS protein was produced essentially as described (19) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and 5% Fetal bovine serum (FBS; Sigma Heat Inactivated, US/HI origin). PBMCs were cultured overnight in chemotaxis media containing RPMI and 1% ultrapure BSA prior to use in assays.
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bioRxiv - Developmental Biology 2023Quote: ... RESCs were derived as reported previously.40 Rat blastocysts were gently flushed out from the E4.5 timed-pregnant rats’ uteruses using M2 medium (Millipore). Zona pellucida was removed with acid Tyrodes solution (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... the epitope tagged LSF was detected by mouse anti-FLAG antibody (F3165, Sigma-Aldrich) and visualized with an anti-mouse IgG coupled with Alexa Fluor 488 (Molecular Probes) ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-EGFP tagged ATP11C was eluted with 0.2 mg/ml Flag peptide (Sigma-Aldrich) in the wash buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
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bioRxiv - Plant Biology 2020Quote: MBP tagged SyYVCV βC1 and mutants were transformed in Rosetta Gammi DE3 cells (Novagen) according to manufacturer’s directions ...
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bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...