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Citations for Millipore Sigma :
551 - 600 of 4259 citations for Recombinant Human NEK7 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... overexpression of GST-β1a was carried out using Escherichia coli BL21 (DE3) (Novagen) transformed with plasmid containing the cDNA inserted into pGEX-4T-2 vector ...
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bioRxiv - Neuroscience 2021Quote: ... the GST tag was cleaved using the Thrombin CleanCleave Kit (Sigma-Aldrich, SLBZ7194) according to the manufacture’s protocol after elution step ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then resolved by SDS-PAGE and immunoblotted using GST (Sigma Aldrich), 6His (Sigma Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... as well as with anti GST antibody (Cat no. G7781-2ML, Sigma, USA). GST protein was used as a control for all the pull-down experiments ...
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bioRxiv - Plant Biology 2021Quote: ... GST-PPKs were tested against 5 µg myelin basic protein (MBP; Sigma Aldrich) and 5 µg Casein in 0.1 M Tris pH 8.8 (Sigma) ...
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bioRxiv - Plant Biology 2022Quote: ... 50 µg GST–CAR9 protein emulsified with Incomplete Freund’s adjuvant (F5506, Sigma–Aldrich) was injected into the ICR mouse and then once again in the next week ...
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bioRxiv - Molecular Biology 2019Quote: ... the slide was incubated with anti-GST antibody (Sigma G7781; 1:13,000 dilution) in 5% dried milk in TBS-T for 1hr at 23°C ...
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bioRxiv - Cell Biology 2020Quote: ... actin 224-236-1 (DSHB Iowa University) and GST clone 6G9C6 (Sigma-Aldrich); rabbit polyclonal anti-GFP/EGFP PABG1 (Chromotek) ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing GST-paFur were cleaved using a Thrombin CleanCleave kit (Sigma-Aldrich). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... A GST-Drn fusion protein was produced in Origami B (DE3) cells (Novagen) and purified with a glutathione-Sepharose 4B column ...
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bioRxiv - Cell Biology 2022Quote: Enzymatic cleavage of the GST tag was performed using thrombin (Cat# T1063, Sigma). Supernatants containing cleaved target proteins were incubated with Benzamidine Sepharose 4 flat flow (Cat# 17-5123-10 ...
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bioRxiv - Cell Biology 2022Quote: ... The expressed GST-fusion protein was absorbed to glutathione-Sepharose 4B (Sigma-Aldrich) followed by extensive washing with phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich). The purified proteins were exchanged to the biotinylation buffer containing 25 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... and recombinant chondriotinase ABC (Sigma, C3667) was treated at 25 mU/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant active AMPK (Millipore, 14-840) was added and incubated for 30 min at 30°C ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant IFNα was purchased from Sigma (Interferon-αA/D human Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... and Recombinant mouse LIF (Sigma, #ESG1107). Additionally ...
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bioRxiv - Immunology 2021Quote: ... Recombinant purified Angiotensin II (Sigma – A9525) at 1 μM with 2-fold serial dilutions ...
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bioRxiv - Immunology 2022Quote: ... recombinant complement component C3 (Millipore Sigma), recombinant CD4 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant leukemia inhibitory factor (SRP3316; Sigma), growth hormone (869008 ...
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bioRxiv - Neuroscience 2023Quote: ... and/or recombinant Fibronectin (Sigma-Aldrich) were applied as described using a range of concentrations (μg/ml).
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bioRxiv - Biophysics 2023Quote: ... superoxide dismutase (bovine SOD, recombinant, Sigma) was added from a ≥50,000 U/mL stock prepared in assay buffer ...
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bioRxiv - Cancer Biology 2023Quote: BG4 recombinant antibody (Sigma Aldrich MABE917)- ChIP
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 mIU/ml recombinant hCG (Sigma), and 10 mg/ml of human serum albumin (Sage) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000U/ml ESGro recombinant LIF (Millipore), 1uM PD0325901 (R&D Systems) ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.25x Protease Inhibitor Cocktail for Histidine-tagged proteins (Sigma-Aldrich), and 0.3 mg/mL lysozyme ...
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bioRxiv - Cell Biology 2021Quote: A549 cells expressing GFP-tagged EGFR were obtained from Sigma. Cells were plated on Edge plates (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... The TAP-tagged proteins were probed with PAP (Sigma-Aldrich) (1:2,000 ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were immunoprecipitated with αFLAG (clone M2, Sigma) beads (Wang et al ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µl protease inhibitor cocktail for histidine tagged proteins (Sigma) / 0.2 g weight of E ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Biochemistry 2022Quote: pCold-GST GTSF-expression vectors were transformed into Rosetta-Gami 2 competent cells (Novagen). Cells were grown in the presence of 1 μM ZnSO4 at 37°C until OD600 ∼0.6–0.8 ...
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bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was incubated with HRP conjugated anti-GST secondary antibody (Sigma –Aldrich, USA) at 1:5000 dilution for one hour ...
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bioRxiv - Cell Biology 2021Quote: ... Western Blot analysis was carried out using Anti-GST(1:3000) antibody (Sigma #G1160) and Anti-His(1:3000 ...
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bioRxiv - Genetics 2020Quote: The GST-Clr4 fusion protein was allowed to bind to Glutathione-agarose beads (Sigma) in MTPBS buffer (100mM Na2HPO4 ...
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bioRxiv - Genetics 2020Quote: L4 gst-4p::gfp expressing worms were anesthetized using 2 mM Levamisole (Sigma L9756) and mounted on 2% agarose pad ...
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bioRxiv - Plant Biology 2023Quote: ... at 1:3,000 dilution or anti- GST primary antibody (cat. no. SAB4200692; Sigma-Aldrich) at 1:2,000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... and GST (#GE27-4577-01, goat polyclonal, Sigma Aldrich; used at 1:10,000 dilution). HRP-conjugated anti-goat and anti-rabbit secondary antibodies (Millipore ...