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Citations for Millipore Sigma :
551 - 600 of 4545 citations for Recombinant Human Hexosaminidase A alpha polypeptide His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-alpha Smooth Muscle Actin-FITC (Sigma, F3777). Secondary antibodies used include goat anti-rabbit FITC ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Microbiology 2023Quote: ... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
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bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...
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bioRxiv - Developmental Biology 2020Quote: ... HA-tagged or Myc-tagged proteins were isolated with anti-HA agarose or anti-c-Myc Agarose Affinity Gel (Sigma), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... GST tagged was bought from Sigma-Aldrich (SRP5172). G-actin and biotinylated actin were bought from Cytoskeleton ...
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bioRxiv - Neuroscience 2021Quote: ... the proteins were tagged with FITC (Sigma, F7250) before encapsulation.
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bioRxiv - Cancer Biology 2022Quote: ... HRP-tagged anti-goat secondary antibody (Sigma-Aldrich), GFP ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-mouse FITC tagged (1:200, Sigma) at room temperature in dark for 90 min ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-His+3AT is minimal medium of SC-His supplemented with 3-aminotriazole (3-AT, 0.5mM, Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Removal of His-tag was verified by western blot with monoclonal α-His –HRP (1:10000) antibody (Sigma).
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bioRxiv - Microbiology 2020Quote: ... HTF-tagged LsaA and FLAG3-tagged VgaALC proteins were detected using anti-Flag M2 primary (Sigma-Aldrich, F1804; 1:10,000 dilution) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μg/ml synthetic alpha-factor (Sigma, St. Louis, MO) were added to logarithmically growing (log-phase ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used were mouse anti-alpha tubulin (DM1A, Sigma) 1:10,000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-sarcomeric alpha-actinin (clone EA53, Sigma-Aldrich, 1:1000), P-Smad1/5/8 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used were alpha-tubulin 1:500 (Sigma T9026), MEK1/2 1:1000 (Cell Signaling 8727s) ...
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bioRxiv - Neuroscience 2020Quote: ... polyclonal antibody against alpha-actinin from Sigma Aldrich (ref SAB4503474), antibody against p62 from Abnova (SQSTM1 monoclonal antibody clone 2C11 ref H00008878-M01) ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-alpha-tubulin as a loading control (Sigma Aldrich). N2a cells were harvested at the same time points for reverse-transcriptase (RT ...
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bioRxiv - Biochemistry 2021Quote: ... we used anti-alpha-tubulin mouse monoclonal antibody from Sigma at 1:10000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... monoclonal mouse anti-Alpha Tubulin (T8203, Sigma; dilution: 1:1000), monoclonal mouse anti-Puromycin (EQ0001 ...
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bioRxiv - Cell Biology 2020Quote: ... alpha-smooth muscle actin (1:150, Cat nb. A5228, Sigma) or NG2 (rabbit ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody against alpha-Tubulin (T9026) was purchased from Sigma-Aldrich. Relative pRSK1 levels were quantified by densitometry using ImageJ.
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-alpha-Tubulin (aTUB) (Sigma-Aldrich #T5168, 1:4000). HRP conjugated secondary antibodies against mouse proteins were obtained from ThermoFisher and used at 1:5000 dilution.
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Expansion of apical extracellular matrix underlies the morphogenesis of a recently evolved structurebioRxiv - Developmental Biology 2019Quote: ... mouse anti-alpha tubulin (acetylated) 1:500 (T6793, Sigma-Aldrich), rat anti-Ecadherin 1:500 (DCAD2 ...
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bioRxiv - Molecular Biology 2019Quote: ... and alpha-cardiac actin (Sigma, cat # A9357, 1:300 dilution) diluted in the blocking buffer ...
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bioRxiv - Bioengineering 2021Quote: ... animals were transitioned to intravenous alpha-chloralose (C0128, Sigma Aldrich; 70 mg/kg induction ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-lys40 acetylated alpha tubulin (1/500, Sigma, #T6793), rat anti-alpha tubulin (1/200 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-alpha-tubulin B-5-1-2 (Sigma, T5168), mouse anti-alpha-tubulin TAT-1 (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... alpha-Smooth muscle actin (mouse IgG2a, clone 1A4, Sigma-Aldrich), and CD26 (rabbit monoclonal ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-alpha tubulin (Merck-SIGMA, clone B-5-1-2) was used as a loading control at 1:10000.
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bioRxiv - Neuroscience 2022Quote: ... and alpha tubulin (α-tubulin; Sigma-Aldrich; T5168; 1:10,000).
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bioRxiv - Neuroscience 2022Quote: ... and the anesthesia was switched to alpha-chloralose (Sigma-Aldrich) administered by continuous infusion via a venous catheter (50 mg/hour/g animal) ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse anti-alpha-tubulin (Sigma-Aldrich, T6074, 1:10,000) were used ...
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bioRxiv - Physiology 2023Quote: ... and mouse anti-alpha-Tubulin (Sigma #T9026, diluted 1:4000) in Odyssey blocking buffer + 0.2% Tween-20 ...
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bioRxiv - Microbiology 2023Quote: ... virus was treated with alpha-1-antitrypsin (Sigma-Aldrich, SRP6312) at the concentration noted ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-sarcomeric alpha actinin (mouse, Sigma-Aldrich A7811; 1/200) anti Twist1 (mouse 1/300 Novusbio NBP2) ...
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bioRxiv - Microbiology 2023Quote: ... or Tumor necrosis factor alpha (TNFα, EMD Millipore, 80054-834) at 10ng mL-1 or vehicle control (DMSO ...
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bioRxiv - Systems Biology 2024Quote: ... and mouse-anti-alpha-tubulin as loading control (Sigma Aldrich, 1:1,000 dilution in Intercept Blocking Buffer) ...
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bioRxiv - Biophysics 2023Quote: ... rabbit anti-alpha-catenin (Sigma Aldrich C-2081, 1:200), and rabbit anti-desmoplakin (abcam ab71690 ...
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bioRxiv - Microbiology 2024Quote: ... and the Bovine IFN-alpha ELISA Kit (Sigma-Aldrich, RAB1012) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-alpha tubulin (1:1000; Sigma, cat no: T6199), mouse anti-gamma tubulin (1:500 ...