Labshake search
Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... N,N,N′,N′-tetrakis-2-Pyridylmethyl-ethylenediamine (Sigma, St. Louis, MO), was also added at a final concentration of 50 µM to prevent non-specific binding of rhod-2 ...
-
bioRxiv - Biochemistry 2024Quote: ... and 0.1 v/v% N,N,N’,N’-tetramethyl ethylenediamine (Sigma Aldrich) was prepared and added to the existing gel layer ...
-
bioRxiv - Biochemistry 2024Quote: ... and 0.1 v/v% N,N,N’,N’-tetramethyl ethylenediamine (Sigma Aldrich) was prepared and casted into 1.00 mm mini-protean glass plates (Biorad) ...
-
bioRxiv - Cell Biology 2021Quote: ... Flag-tag (Sigma), Myc-tag (Upstate) ...
-
bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...
-
bioRxiv - Neuroscience 2024Quote: ... N,N′-methylbisacrylamide (Sigma), sodium chloride (Fisher) ...
-
bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
-
bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
-
bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
-
bioRxiv - Bioengineering 2020Quote: ... 25 wt% N,N,N’,N’-tetrakis(2-hydroxypropyl) ethylenediamine (Sigma Aldrich, 585714), and 5 wt% Triton X-100 (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2022Quote: ... with 100 μM N,N,N’,N’-tetramethyl-ρ-phenylene diamine (Sigma-Aldrich). Glycolytic and mitochondrial ATP production rates were determined according to the XF Real-Time ATP Rate Assay Kit protocol (Agilent) ...
-
bioRxiv - Bioengineering 2023Quote: ... 25 wt% N,N,N′,N′-tetrakis (2-hydroxypropyl)ethylenediamine (Sigma-Aldrich, 122262) and15 wt% Triton X-100 in 0.01 M PBS ...
-
bioRxiv - Bioengineering 2024Quote: ... freshly prepared 10% (w/w) N,N,N′,N′-tetramethylethylenediamine (TEMED, Sigma, T7024) and 10% (w/w ...
-
bioRxiv - Cell Biology 2024Quote: ... N,N,N’,N’-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine (TPEN, P4413, Sigma-Aldrich), 1,2-bis-(aminophenoxy)- ethane-N,N,N’,N’-tetra-acetic acid-acetoxymethyl ester (BAPTA-AM ...
-
bioRxiv - Neuroscience 2023Quote: ... 25 wt% N,N,N′,N′-tetrakis (2- hydroxypropyl)ethylenediamine (Sigma-Aldrich, 122262) and 15 wt% Triton X-100 (AppliChem ...
-
bioRxiv - Bioengineering 2023Quote: ... we added 2.6 μL of N,N,N′,N′-tetramethylethylenediamine (TEMED; Sigma, 411019) and 260 μL of the 10% w/v APS solution to the PA solution ...
-
bioRxiv - Biochemistry 2021Quote: ... Carboxyl-terminal histidine or N-terminal glutathione-S-transferase fusion tags were added using the respective plasmids pET21b (Novagen, Madison, Wisconsin, USA) and pGEX-4T3 (Amersham Biosciences ...
-
bioRxiv - Microbiology 2020Quote: ... acidophilum were PCR-amplified from their respective genomic DNA template and inserted into a pET16b vector containing 10x histidine tag at the N-terminus (Novagen, Madison, WI). The following primers were used ...
-
bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
-
bioRxiv - Bioengineering 2024Quote: Recombinant active human MMP-9cd (auto-cleavage resistance) 34 with an N-terminal His6x tag was expressed in Rosetta™(DE3) pLysS (Millipore Sigma) cells using a pET-28a(+)-His6x-MMP-9cd vector as previously described 33,35,36 ...
-
bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 5% skimmed milk in Tris Buffered saline-Tween for 1 h before incubation with primary antibody anti-myc tag monoclonal clone 4A6 (Millipore) diluted to final concentration of 200 ng mL−1 in 3% skimmed milk in TBST for 1h at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
-
bioRxiv - Cancer Biology 2022Quote: ... N-methyl-N’-nitro-N-nitrosoguanidine (MNNG; Sigma-Aldrich), and bleomycin (Pharmachemie) ...
-
bioRxiv - Cell Biology 2022Quote: ... FLAG tag antibody (1:1000 dilution used) raised in mouse (F1804) was obtained from Sigma-Aldrich and Porin antibody (1:5000 dilution used ...
-
bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
-
bioRxiv - Microbiology 2024Quote: ... and incubated with a mouse monoclonal antibody [M2] specific to the FLAG-tag (Sigma-Aldrich, F1804) in 1% BSA/PBS at 37℃ for 1 hour ...
-
bioRxiv - Cell Biology 2023Quote: ... the membranes were probed with primary antibodies against FLAG-tag (dilution 1:1000, mouse; Sigma, A1804), Wee1 (dilution 1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and mouse a-mouse IDO1 (clone 8G11, Merk) were conjugated with either PLUS (#DUO92009, Sigma-Aldrich) or MINUS (#DUO92010 ...
-
bioRxiv - Neuroscience 2022Quote: ... or rabbit anti-DAT (1:400) (clone 1D2 ZooMAb, n°: ZRB1525, Sigma Aldrich, St. Louis, MO, USA) and secondary (goat anti-rabbit Alexa-594 (1:500 ...
-
bioRxiv - Cell Biology 2024Quote: ... His-3C was depleted on the His-Select Affinity matrix (Sigma-Aldrich) prior to Size Exclusion Chromatography on a HiLoad 16/60 Superdex200 column (Cytiva ...
-
bioRxiv - Cell Biology 2020Quote: ... and 1ul of N,N,N′,N′-Tetra-methyl-ethylenediamine accelerator (TEMED, Sigma-Aldrich) per ml of solution was added ...
-
bioRxiv - Cell Biology 2022Quote: ... N,N,N′,N′-Tetramethyl-p-phenylenediamine [TMPD (T7394)] were purchased from Sigma-Aldrich. HPLC-grade acetonitrile (A955) ...
-
bioRxiv - Biophysics 2020Quote: Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA, 03777, Sigma), 10% FBS (Sigma ...
-
bioRxiv - Developmental Biology 2020Quote: ... 25% (w/w) N,N,N’,N’-tetrakis(2-hydroxypropyl)ethylenediamine (Sigma-Aldrich 122262) and 15% tritonX-100 (Sigma-Aldrich X-100 ...
-
bioRxiv - Physiology 2023Quote: ... and N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride (TMPD, 0.5mM; Sigma-Aldrich, 87890), with prior inhibition with Antimycin A (AMA ...
-
bioRxiv - Neuroscience 2024Quote: ... TEMED (N,N,N′,N′-Tetramethylethylenediamine at 10% (v/v) (Sigma-Aldrich, Cat# T7024), and ammonium persulfate at 10% (w/v ...
-
bioRxiv - Immunology 2022Quote: ... mouse monoclonal IgG2aĸ antibody with epitope matching the N-terminus (EMD Millipore #MABS1920), nuclear matrix protein p84 [EPR5662(2)] rabbit monoclonal antibody mapping with aa350-450 (abcam #ab131268) ...
-
bioRxiv - Cell Biology 2023Quote: ... N,N-methylenebisacrylamide (Sigma, M1533) 0.1% in 1x PBS ...
-
bioRxiv - Bioengineering 2024Quote: ... N,N’-diisopropylcarbodiimide (DIC, Sigma), poly(ethylene glycol ...
-
bioRxiv - Plant Biology 2024Quote: ... 0.15% N,N′-methylenebisacrylamide (Sigma), 8.625% sodium acrylate (Interchim ...
-
bioRxiv - Biochemistry 2022Quote: ... containing N,N’-methylenebisacrylamide (Sigma) in a 19:1 ratio in equal volume ...
-
bioRxiv - Cell Biology 2020Quote: ... Mouse monoclonal antibody against actin (clone C4) was from Millipore (#MAB1501). Rabbit polyclonal antibody against NFATc3 was from Proteintech (18222-1-AP) ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-talin (T3287, mouse monoclonal, clone 8D4, lot 035M4805V; Sigma-Aldrich), anti-GEF-H1 (ab155785 ...
-
bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-α-tubulin (Sigma, clone DM1A, T6199, 1:25’000) and mouse monoclonal anti-GAPDH (Santa Cruz ...
-
bioRxiv - Molecular Biology 2020Quote: ... monoclonal mouse anti-phospho-Histone H2A.X (Ser139) antibody (Millipore, clone JBW301) and monoclonal mouse anti-β-actin (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse IgG1 anti-Pan Nav (clone K58/35; 1:150; Sigma); mouse anti-Calbindin (1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-MAP2 (clone AP-20; Sigma-Aldrich; RRID: AB_477171), rabbit polyclonal anti-MAP2 (Abcam ...
-
bioRxiv - Microbiology 2021Quote: ... Parasites were labelled with anti-Flag mouse antibodies (M2 clone, Sigma) and AlexaFluor 594-conjugated secondary antibodies (Life Technologies) ...
-
bioRxiv - Microbiology 2021Quote: ... mouse anti-p21 (clones CP36 and CP74, 1:1,000; Millipore Sigma), and rabbit anti-SOX2 (1:1000 ...
-
bioRxiv - Cancer Biology 2020Quote: Mouse monoclonal anti-Tubulin (clone DM1A) (Sigma-Aldrich Cat # 05-829)