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Citations for Millipore Sigma :
551 - 600 of 7883 citations for Lassa Fever Virus GP1 Protein Sheep Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... blocking of Fc receptors with human Ig (Sigma-Aldrich, St. Louis, MO); surface staining with mouse anti-human CD3-APC-H7 ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). For analysis of neutrophil activation ...
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bioRxiv - Cell Biology 2023Quote: ... was mixed with unconjugated Fc-antibody (5 µg/ml, Sigma-Aldrich, # I2136) in HBSS and incubated for 30-60 minutes at room temperature with gentle agitation ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). To enrich for MSCs ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). After blocking ...
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bioRxiv - Cancer Biology 2023Quote: ... Hybridoma cells were selected in DMEM/10% FCS containing HAT (Sigma, MO) and 1% BM-Condimed (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich; St. Louise, MO), penicillin streptomycin glutamine (100 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Biochemistry 2024Quote: ... the supernatant supplemented with 1% Pen/Strep and 10% FCS (Sigma-Aldrich) and stored in the dark at 4°C.
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bioRxiv - Bioengineering 2024Quote: ... or horseradish peroxidase-conjugated goat anti-human IgG Fc antibody (Sigma, A0170) diluted 1:20,000 in the blocking buffer for an hour at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 5-16-day old females were fed sheep blood supplemented with 2 mM adenosine 5’-triphosphate (ATP) (Sigma Aldrich, A6419) in aqueous sodium bicarbonate buffer using a new artificial membrane feeder called the blood puck ...
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bioRxiv - Developmental Biology 2022Quote: ... washed in PBS with 0.1% Triton X-100 (PBTr) and blocked with the addition of 10% sheep serum (Sigma Aldrich). Primary antibodies used were ...
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bioRxiv - Physiology 2020Quote: ... Immunofluorescence staining was performed using sheep anti-α-MSH antibody (diluted 1:1,000 in 0.1 M PB, ab5087, Millipore Sigma), and rabbit anti-AgRP antibody (diluted 1:1,000 in 0.1 M PB ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed with TBST wash buffer (10% Tween20 in 1xTBS buffer) and incubated in 10% sheep serum (Sigma Aldrich) in TBS-1%BSA (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Cell Biology 2020Quote: ... Virus stock was filtered by centrifugation using Amicon Ultra-15 Centrifugal filter unit (Sigma, Cat # UFC910096) and resuspended in viral propagation media ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting supernatant or ‘secondary virus’ was filtered using 0.45 μm pore size sterile filter (Millipore) and was used immediately to infect large-scale expression cultures ...
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bioRxiv - Cell Biology 2022Quote: ... the medium containing the virus particles was recovered and concentrated with Polyethylene Glycol 8000 (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... cells were infected with serial dilutions of virus diluted in 1× minimum essential medium (MEM, Sigma) supplemented with 0.6% BSA (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... HeLa cells were incubated overnight in growing media plus virus and polybrene (Sigma-Aldrich, TR-1003). The following day ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant was collected after 48hrs and 72hrs and virus was concentrated using Polyethylglycol (PEG, Sigma # P4338). V6.5 cells were infected in medium containing 5ug/ml polybrene (Millipore ...
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bioRxiv - Microbiology 2021Quote: Virus propagation was performed in Vero E6 cells in DMEM (Sigma-Aldrich, St Louis, MO, USA) supplemented with 2% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... FFUs were visualized using MAB8705 Anti-dengue Virus Complex Antibody clone D3-2H2-9-21 (Millipore) and goat anti-mouse Alexa Fluor 488 (A-11001 ...
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bioRxiv - Cell Biology 2019Quote: ... the virus containing suspension from HEK293T cells was filtered with a 0.45 μM filter (EMD Millipore), supplemented with polybrene (4 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... Virus was added at the indicated titer in the presence of polybrene (2µg/mL, Sigma, H9268) and the cells were incubated for 24 h ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... All virus propagation was performed in VeroE6 cells cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... The virus containing supernatant was mixed with polyethylene glycol 6000 (Sigma Aldrich, Saint Louis, Missouri, US) (8 % wt/v ...
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bioRxiv - Systems Biology 2022Quote: ... virus particles were precipitated by adding 50% (wt/vol) polyethylene glycol 6000 (PEG 6000) (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... culture supernatants containing virus were harvested and passed through a 0.45 μM PVDF filter (Millipore, SLHV033RS). Viral titers were calculated and an MOI of 1 was used for transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... and concentrated using fast-trap virus purification and concentration kit according to manufacturer′s instructions (Millipore). For transduction ...
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bioRxiv - Microbiology 2024Quote: ... the virus/glycan-mixture is incubated with 0.5 U heparinase I and III (H3917, Sigma-Aldrich) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... samples were incubated in L-Cry antibodies 5E3-3E6-E8 (1:100) and 4D4-3E12-E7 (1:50) in 5% sheep serum (Sigma-Aldrich). Secondary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... Larvae were blocked in 10% sheep serum in PBDT for 1 hour at room temperature and then incubated in mouse anti-alpha acetylated tubulin (Sigma-Aldrich Canada Ltd ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The 1% BSA in PBST was then used to dilute sheep anti-digoxygenin Fab-fragments conjugated to alkaline phosphatase (1:1000, Sigma-Aldrich) in which the tissues were incubated overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The specimens were incubated at room temperature for 1 h in blocking buffer (PBSTX with 10% normal sheep serum and 2% bovine serum albumin) and subsequently with the primary antibody (rabbit-anti-GABA, Sigma-Aldrich A2052 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by primary antibody incubation in 10% sheep serum at 4°C for 3 days (Anti-SCN5A antibody produced in rabbit (Sigma SAB2107930) 1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... a blocking step of incubation with sheep serum for 1 hour was followed by immunostaining for N using a mouse monoclonal antibody (αN009 48) and detected using a sheep anti-mouse FITC conjugate (Sigma, UK). Phalloidin Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... in a mixture of two monoclonal antibodies against L-Cry, 5E3-3E6-E8 and 4D4-3E12-E7 (1:100 and 1:50, correspondingly, in 5% sheep serum (Sigma-Aldrich)) (see accompanying manuscript for further details) ...
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bioRxiv - Biochemistry 2022Quote: ... HRP-conjugated secondary antibody (HRP-link anti-mouse IgG, Cell Signaling, 1:10 000 dilution for C-terminal Parkin Ab and HRP-link anti-sheep IgG, Sigma, 1:10 000 dilution for N-terminal Parkin Ab ...
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bioRxiv - Immunology 2020Quote: ... were washed twice with 1 mL of buffer and sensitised with a 1/1000 dilution of rabbit anti-sheep red blood cell stroma antibody (Sigma; S1389). After a 30 °C / 30 minutes incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos after in situ HCR were washed with PBST and soaked in blocking solution [5% heat inactivated (56°C, 30 min) sheep serum (S2263, Sigma-Aldrich) in PBST] at room temperature for 2 hours ...
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bioRxiv - Physiology 2023Quote: ... Collected adrenal sections were probed with antibodies for TLQP-21 (rabbit anti-TLQP-21, 1:100, 20) or Tyrosine Hydroxylase (sheep polyclonal TH, 1:100, Millipore Sigma) overnight at 4°C in a humidified chamber ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was carried out in freshly made blocking buffer (5% FCS, Sigma-Aldrich, Gillingham ...
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bioRxiv - Immunology 2022Quote: ... in 10 ml VLE DMEM (Biochrom) containing 10% heat-inactivated FCS (Sigma-Aldrich), 0.1% 2-mercaptoethanol (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Spleens were gently meshed in FACs buffer (PBS containing 3% FCS from Sigma) using a 40μM cell strainer to prepare single cell suspensions ...
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bioRxiv - Molecular Biology 2019Quote: ... MDCKII cells were grown in DMEM + 10%FCS + Antibiotic/Antimycotic solution (Sigma A5955)(Williams et al. ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 10% FCS tested to be dox-free (Sigma-Aldrich, Taufkirchen, Germany) and penicillin and streptomycin (final concentrations 100 units/ml and 100µg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...