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Citations for Millipore Sigma :
551 - 600 of 878 citations for Ebola Virus Envelope Glycoprotein GP Zaire Kikwit 95 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were denatured for 5 min at 95 °C in Bolt LDS Sample buffer with 5 % v/v 2-mercaptoethanol (Sigma). 10-30 μg of proteins were loaded in Bolt 4-12 % Bis-Tris gels (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... 95 μL of lysed and sonicated equine SF–EV were treated with 5 mM dithiothreitol (DTT) (Sigma–Aldrich, Dorset, UK) 100 mM at 60°C and shaken at 1000 rpm for 30 mins on an orbital shaker ...
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bioRxiv - Neuroscience 2022Quote: ... The pH was adjusted to 7.35 when bubbled with 95% O2/5% CO2 using 1 M KOH (Fluka Analytical, Sigma-Aldrich). After cutting ...
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bioRxiv - Physiology 2023Quote: ... C2C12 cells were grown to 95 % confluency followed by differentiation with DMEM Glutamax (supplemented with 2% v/v horse serum (Sigma) and 1 % v/v PenStrep) ...
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bioRxiv - Molecular Biology 2023Quote: ... we cleaved the peptides from beads with standard TFA cleavage cocktail (comprising 95% TFA, 2.5% water and 2.5% Triisopropylsilane (Sigma, Cat #233781) for 2-4 hours at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5 µl of the reaction mix were added to 95 µl 5 mM Iodonitrotetrazolium chloride which is a cheaper alternative to WST-1 (INT, Sigma) and 5 µg/ml mPMS in DMSO ...
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bioRxiv - Biophysics 2024Quote: C2C12 cells were cultured at standard incubation conditions (37 °C, 5% CO2, 95% humidity) in Dulbecco’s modified Eagle medium supplemented with 15% fetal bovine serum (Sigma-Aldrich), 2% penicillin-streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: ... hard baked at 95°C for 5 to 10 min and developed in propylene glycol monomethyl ether acetate (Sigma Aldrich). For the microfluidic devices ...
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bioRxiv - Cell Biology 2020Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45µm filter (EMD Millipore), and used for transduction of 4T07 ...
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bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(-) ...
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bioRxiv - Cancer Biology 2020Quote: Vesicular stomatitis virus (VSV)-G protein pseudotyped lentiviruses and retroviruses were prepared by using polyethylenimine (PEI; Sigma) to co-transfect 293T cells with a lentiviral or retroviral plasmid and pantropic VSVg (Addgene ...
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bioRxiv - Cell Biology 2019Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2µg/ml Puromycin (Sigma-Aldrich). After 7 days of selection ...
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bioRxiv - Microbiology 2020Quote: ... Airway Chips infected with influenza virus (MOI = 0.1) were treated with 1 µM oseltamivir acid (Sigma-Aldrich) under flow (60 µl/h ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45μm filter (EMD Millipore) and used for transduction of MCF-10A cells in the presence of polybrene (8μg/mL) ...
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bioRxiv - Neuroscience 2021Quote: Each animal (with DREADD virus injection) received CLZ (0.5 mg/kg, Sigma-Aldrich, dissolved with 0.1% DMSO) or vehicle (sterilized saline with 0.1% DMSO ...
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bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... pLVX-EF1α-mKate2-WPRE-Neo virus supplemented with 10 µg/ml polybrene infection/transfection reagent (Sigma-Aldrich) was incubated with PSCs grown in monolayer for 24 h ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... virus was also concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). Mice were injected s.c ...
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bioRxiv - Microbiology 2021Quote: ... and 10% formamide in DEPC-treated H2O) with anti-influenza A virus NP antibody (Millipore, 1:2,000) and FISH probes ...
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bioRxiv - Bioengineering 2021Quote: ... cells were transduced with 4 µL library virus/well along with 8 µg/mL Polybrene (Sigma-Aldrich) aiming for an MOI at 0.3-0.4 (Supplementary Methods and Results) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultivated with the virus in the presence of 4 μg/ml polybrene (Sigma-Aldrich) for 12 hr.
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with virus (MOI 0.5-1.0) and 5 µg/mL Polybrene (EMD Millipore, TR-1003). Polyclonal cells were selected for 2 weeks in 250 µg/mL G418 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Virus was then removed and cells were overlayed with 3 mL of DMEM with 0.8% methylcellulose (Sigma), 1% FBS ...
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bioRxiv - Physiology 2020Quote: Virus carrying the gene of interest was used to infect cell lines with 10mg/mL polybrene (Millipore). Media was replaced after 24 h and cells were sorted for GFP positivity after 48-72 h post-infection ...
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bioRxiv - Genetics 2019Quote: ... The resulting myoblasts were then infected with a retroviral cocktail containing Myf6-CTAP virus and polybrene (Millipore) for 8 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Suspensions of L-cells (from ATCC) for virus production were maintained in Joklik MEM medium (Sigma-Aldrich) supplemented with 1% L-Glutamine (Gibco.) ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant containing the virus was collected after 72 hours and filtered through a 0.22 micron PES (Millipore) filter ...
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bioRxiv - Genomics 2021Quote: ... Harvested media containing the desired virus were filtered through Millex-HV 0.45-µm PVDF filters (Millipore, SLHV033RS) and further concentrated with 100,000 NMWL Amicon Ultra-15 centrifugal filter units (Amicon ...
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bioRxiv - Cell Biology 2021Quote: ... supernatant containing propagated virus was filtered through an Amicon Ultra 15 (100 kDa) centrifugal filter (Millipore Sigma) at ~4000 rpm for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... virus was purified using centrifugal filter devices (Centricon Plus-70 and Biomax 100.000, Millipore Corp., Bedford, MA) and stocks with a titre between 3×109 and 3×1010 viral genome equivalents (vge ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa or U2OS cells were transduced with virus for 48 h with 10 μg/ml polybrene (Sigma), then optimized for protein expression via fluorescence sorting or puromycin selection.
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus containing media were harvested 48-64 h later and filtered by 0.45 μm Steriflip filter (Millipore). HepG2 and Huh7 cells were infected with 1 ml virus containing medium with 8 μg/ml polybrene for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was replaced with virus-containing medium supplemented with 8 µg/mL of polybrene (Sigma-Aldrich). Approximately 24 hours post-viral treatment ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Immunology 2022Quote: ... The supernatant containing virus particles was collected after 48h and filtered via 0.45 μm filter (Roth/Millipore). Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15-Cas9 cells were infected with pooled CRISPR library virus using polybrene (8 µg/ml, Sigma) using a low multiplicity of infection (MOI = 0.75 ...
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bioRxiv - Microbiology 2023Quote: ... RNA from the supernatant of cell culture infected with the virus was isolated using the Trizol (Sigma) method described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... NF1-MET cells were infected with 500 μL of 0.45 μm filtered virus with polybrene (EMD Millipore), and 48 hours post infection transduced cells were selected for by 2 μg/mL puromycin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus was removed and replaced with normal growth medium containing 1 mg/ml puromycin from Sigma. The puromycin selection was used to kill off any uninfected control cells ...
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bioRxiv - Microbiology 2023Quote: ... the virus suspension was replaced with 1mL/well of overlay medium (OM) containing 1% methylcellulose (Sigma, USA) in MM ...
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bioRxiv - Microbiology 2024Quote: ... 100 TCID50 of the virus in Intesticult media supplemented with 500 μM sodium glycochenodeoxycholate (GCDCA; Sigma, G0759) was added to 5-day differentiated HIE monolayers in triplicate on a 96-well plate ...
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bioRxiv - Bioengineering 2021Quote: ... a 95/5 L-lactide/DL-lactide copolymer (Corbion) 2% m v−1 solution in dry 1,4-dioxane (Sigma-Aldrich, 296309) was spin-coated at 3000 rpm for 30 s on 1.25 × 1.25 cm Corning® glass microslides (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: ... Eyes were enucleated from euthanized mice (sodium pentobarbital, 150 mg kg−1) and dissected in carboxygenated (95% O2 and 5% CO2) Ames’ medium (A1420, Sigma-Aldrich) under dim red light ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 15 minutes in 95% and 100% ethanol and 5 minutes in hexamethyldisilazane (HMDS, Sigma-Aldrich, Merck, St Louis, USA). Samples were left to air-dry overnight in a biosafety cabinet prior to mounting on electron microscope stubs covered with carbon tape ...