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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Acetamide N 2 3 dihydro 2 3 hydroxy 2 quinolinyl 1 3 dioxo 1H inden 5 yl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2 6 H2O and 5-Bromo-4-chloro-3-indolyl β-D-galactoside or X-gal (Sigma, B4252 ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, San Louis, MI, USA) solution in PBS was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Microbiology 2023Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Microbiology 2023Quote: ... 30 μL of MTT [(3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl-2H-tetrazolium bromide; 5 mg/mL; (Sigma-Aldrich)] was added in each well and the plate incubated for 3 hours at 25°C ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was applied to the cells and incubated for an additional 4h ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were washed 3 times (5 minutes each) in wash buffer DPBS Tween containing 2% BSA (Sigma, A3311), and permeabilised at room temperature for 20 minutes in permeabilisation buffer 0.5% Triton-X in DPBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... media was aspirated from cells and replaced with 5 mg/mL 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, #M5655, Sigma Aldrich) solution in base media ...
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bioRxiv - Immunology 2024Quote: ... the allosteric FFA2R modulator Cmp58 ((S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide) and adenosine 5′-thriphosphate disodium salt hydrate (ATP) were from Sigma-Aldrich (Merck, Burlington, MA, USA). The FFA2R antagonist CATPB ((S)-3-(2-(3-Chlorophenyl)acetamido)-4-(4-(trifluoromethyl ...
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bioRxiv - Microbiology 2021Quote: ... sporozoites were added to 1 ml molten 3% agarose (2-Hydroxyethyl agarose – Sigma-Aldrich, dissolved in ddH2O), centrifuged and incubated at 4°C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... For cell treatments the following compounds were used: 2′,3′-Dideoxycytidine (1-10 μM, Sigma-Aldrich®), Ethidium bromide (50 ng/mL ...
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bioRxiv - Biochemistry 2020Quote: ... phosphatidyl-choline (2-oleoyl-1-palmityl-sn-glycero-3-phosphocholine) were from Sigma-Aldrich (St. Louis, MO); Ezetimibe and lysophosphatidylcholine (1-palmitoyl-sn-glycero-3-phosphocholine ...
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bioRxiv - Developmental Biology 2020Quote: ... Phosphopeptide standards (0.1 pmol of MS PhosphoMix 1, 2, 3 Light; Sigma-Aldrich, St. Louis, Missouri, USA) was added to suspended sample in binding/equilibration buffer ...
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bioRxiv - Biochemistry 2021Quote: ... coli polar lipids (Avanti, US) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) (Sigma Aldrich) in a 3:1 molar ratio ...
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bioRxiv - Neuroscience 2024Quote: ... 6-diamidino-2-fenylindol (DAPI; 1:10 000; Sigma-Aldrich, Saint-Louis, MO, USA, 28718-90-3) and phosphate buffer (PB ...
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bioRxiv - Neuroscience 2024Quote: ... 6-diamidino-2-fenylindol (DAPI; 1:10 000; Sigma-Aldrich, Saint-Louis, MO, USA, 28718-90-3) and phosphate buffer (PB ...
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bioRxiv - Molecular Biology 2021Quote: ... MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide] dye solution (Sigma-Aldrich, USA) (5 mg MTT in 1 ml PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma-Aldrich) and phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich). Protein concentration was measured with BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... The coverslips were then immersed in 2% (v/v) (3-Aminopropyl)triethoxysilane (Sigma A3648) prepared in acetone for 2 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Cells were split every 2-3 days with trypsin (Sigma, Saint Louis, Missouri, USA) to maintain a subconfluent monolayer.
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bioRxiv - Cell Biology 2020Quote: ... Sigma-Aldrich) and phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich). Protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... concentrated to 2 ml using an Amicon concentrator with a 3 kDa cutoff (Millipore), and applied into a Superdex 75 (16/600 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 2× M63 buffer was made by adding 3 g KH2PO4 (Sigma-Aldrich cat. P5655), 7 g K2HPO4 (Sigma-Aldrich cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were subcultured every 2-3 days using trypsin (Sigma, Saint Louis, Missouri, USA) to maintain a subconfluent cell layer.
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bioRxiv - Biochemistry 2020Quote: Ebselen (2-Phenyl-1,2-benzisoselenazol-3(2H)-one) is commercially available from Sigma Aldrich.
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1X protease inhibitor and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich), for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: 3-4 nl of 2 mg per mL Rhodamine B isothiocyanate-Dextran (70kDa, Sigma) dissolved in PBS and 5 mM HEPES were injected into the sinus venosus of 30 hpf embryos ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 3 μM SHP1/2 phosphatase inhibitor (NSC-87877, Sigma-Aldrich) for 20 minutes prior to target particle addition ...
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bioRxiv - Genetics 2020Quote: ... Cells were washed with 3 × 100 µL of 2% bovine serum albumin (BSA, Sigma) in D-PBS+CM and blocked in that same solution for 2 hours at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... 540-69-2] (pH 3, adjusted with formic acid [Sigma Aldrich, 64-18-6]) and solvent B was acetonitrile [Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... and 1.0% (v/v) phosphatase inhibitor cocktail #2 and #3 (Sigma-Aldrich P5726, P0044). Tissue homogenates were centrifuged at 14,000×g at 4°C for 20min and supernatants collected and stored at -80°C before initiating the protein assay.
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were transformed into pKM208 carrying Rosseta 2 (Millipore Sigma 71400-3) strain by electroporation ...
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bioRxiv - Developmental Biology 2022Quote: Myoblasts were transfected with either (final concentrations) 10 µM 2’,3’-cGAMP (Sigma Aldrich), 2 µg/mL poly(I:C ...
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bioRxiv - Microbiology 2022Quote: ... coverslips were incubated in a 2% solution of (3-Aminopropyl)triethoxysilane (APTES, Sigma-Aldrich) in acetone with agitation for two minutes ...
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bioRxiv - Cell Biology 2022Quote: MCF10A cells (3×106 cells per condition) were fixed with 2% formaldehyde (Sigma, #252549) in PBS with 10% FBS for 10 min at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and Phosphatase Inhibitor Cocktails 2 and 3 (Sigma- Aldrich cat. P5726 and cat. P0044). Pellets were resuspended in lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... and 2’,3’-cGAMP (CAS no. 1441190-66-4) were obtained from Sigma-Aldrich and used without further purification.
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bioRxiv - Molecular Biology 2024Quote: ... 100 μL each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044)) and lysate cleared by centrifugation at 17,000g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... [3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine sulfonate] FerroZine (FZ) (Sigma-Aldrich, Bangalore, India) was added to create Fe-deficient conditions ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed at room temperature for 2 hours with 3% BSA (Sigma-Aldrich) PBS-Tween ...
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bioRxiv - Biophysics 2023Quote: ... different amounts of freeze dried GelMA macromer were dissolved in DPBS containing 0.5% (w/v) 2-Hydroxy-4’-(2-hydroxyethoxy)-2- methylpropiophenone (Sigma-Aldrich) as photoinitiator ...
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bioRxiv - Biochemistry 2021Quote: ... 6-Formylindolo[3,2-b]carbazole (FICZ) and 2-Methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) from Sigma-Aldrich. 1-HP ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Cell Biology 2020Quote: ... BSA was coupled to the PEG (3000) for 1h at RT with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 100 mM, Sigma Aldrich, 39391) and N-hydroxy-succinimide (NHS ...
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bioRxiv - Microbiology 2023Quote: ... Purified 3’-Sialyl-N-acetyllactosamine (Sigma-Aldrich) was diluted in PBS to 100 μM ...
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bioRxiv - Synthetic Biology 2023Quote: ... n- (3-hydroxytetradecanoyl)-HSL (Sigma-Aldrich, #51481), and p-coumaroyl-HSL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... or 3’-sialyl-N-acetyllactosamine (Sigma-Aldrich). N-acetyl-D-lactosamine was prepared in PBS to a concentration of 1000 µM and diluted 2-fold from 1000 µM to 62.5 µM ...