Labshake search
Citations for Millipore Sigma :
551 - 600 of 10000+ citations for 6 PHENETHYLCARBAMOYL CYCLOHEX 3 ENECARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2 6 H2O and 5-Bromo-4-chloro-3-indolyl β-D-galactoside or X-gal (Sigma, B4252 ...
-
bioRxiv - Cancer Biology 2020Quote: ... MD2G (3 μg) and pLKO-LV-gene-specific (6 μg) plasmids in the presence of PEI transfection reagent (Sigma). The generated lentiviral particles were collected 24 hours post-transfection ...
-
bioRxiv - Plant Biology 2023Quote: ... spy-3 and spy-4 were sprayed with either 50 µM or 100 µM BA (6-benzylaminoadenine, Sigma B3408) or mock [200 mM NaOH] three times ...
-
bioRxiv - Microbiology 2023Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre- soaked for 5 minutes in room temperature 0.5X TBE ...
-
bioRxiv - Microbiology 2022Quote: ... embryos were mechanically dechorionated if necessary and anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The bacterial suspension was then injected into the hindbrain ventricle of casper zebrafish larvae at 2 dpf ...
-
bioRxiv - Neuroscience 2021Quote: ... the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 10 μM, Sigma-Aldrich) and a selective group III metabotropic glutamate receptor agonist ...
-
bioRxiv - Microbiology 2019Quote: ... were first floated on ultrapure water for 5 min before incubation in blocking buffer (1xPBS containing 0.1%v/v Tween-20 and 3% fatty-acid free BSA (Sigma A7030)) at RT for 1h ...
-
bioRxiv - Biochemistry 2020Quote: ... nicotinic acid (cat. N4126), 2-aminopyridine-3-carboxylic acid (cat. A68300) and 1,1′-Carbonyldiimidazole (CDI, cat. 21860) were purchased from Sigma Aldrich. For the synthesis of NAI ...
-
bioRxiv - Plant Biology 2020Quote: ... To remove residual boric acid the medium was treated overnight with B chelator Amberlite IRA-743 (3 g/L, Sigma). Before autoclaving ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were replica-plated onto pABA− (2% glucose, w/v) plates with 0 µM or 25 µM linolenic acid (C18:3, Sigma). Colonies that grew on 25 µM linolenic acid were picked into YEPD overnight cultures and struck on YEPD plates ...
-
bioRxiv - Biochemistry 2022Quote: ... coli strain MG1655-seeded NGM plates in the presence or absence of 1 mM auxin (IAA, indole-3-acetic acid, Sigma).
-
bioRxiv - Plant Biology 2022Quote: ... and sterilized for 3 h in a mixture of bleach (30 mL) and concentrated hydrochloric acid (1.5 mL, Sigma-Aldrich). The pgb1-1 mutant lacks ethylene-induced PGB1 expression and lower hypoxia induced PGB1 expression (relative to wild-type ...
-
bioRxiv - Genetics 2022Quote: ... Degradation of the AID-tagged RAD21 was induced with addition of 500 μM indole-3-acetic acid (auxin; Sigma-Aldrich). Degradation of AID-tagged WAPL was induced with addition of 1μM 5-Ph-IAA (modified auxin ...
-
bioRxiv - Cell Biology 2020Quote: ... were blocked for 1h in TBS containing 0.1% Tween-20 (TBS-T) supplemented by 3% fatty-acid free BSA (Sigma Aldrich) before incubation with the purified STARD10 protein (1 μg/mL in TBS-T + 3% BSA ...
-
bioRxiv - Neuroscience 2021Quote: ... larvae were anesthetized with 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) and mounted laterally in 2% low-melting temperature agarose (Thermo Fisher Scientific 16520) ...
-
bioRxiv - Microbiology 2022Quote: ... Calu-3 cells media was instead supplemented with a 1x final concentration of non-essential amino-acids (Sigma cat. M7145) and sodium pyruvate (Gibco cat ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were arrested in G1 as above and 1 h prior to release auxin (indole-3-acetic acid sodium salt; Sigma) was added to a final concentration of 1 mM ...
-
bioRxiv - Molecular Biology 2019Quote: ... degradation of V5-AID-fused Brn1 protein was induced by addition of 0.5 mM indole-3-acetic acid (Auxin, Sigma # 13750).
-
bioRxiv - Biochemistry 2020Quote: The pure protein recovered after HPLC purification in 1% acetic acid was concentrated in Amicon Ultra-15 (cut-off 3 kDa) centrifugal filter units (Merck Millipore) to reach the final protein concentration of 1 mM ...
-
bioRxiv - Microbiology 2021Quote: ... mobile phase and stained using ethanol: sulfuric acid (70:3 v/v) solution containing 1% (w/v) orcinol monohydrate (Sigma). HPAEC-PAD samples were diluted to 50 μM and resolved on a Dionex PA20 column in a mobile phase of 30 mM NaOH and gradient of 10 mM – 120 mM sodium acetate over 40 min at a flow rate of 0.5 mL min-1 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Colonies were fixed with Carnoy’s solution (1 acetic acid: 3 methanol) and stained with 1% (w/v) crystal violet (Sigma-Aldrich). The percentage of colonies covering the plates were calculated with ImageJ software.
-
Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: ... The bound peptides were eluted 3 times from the beads with 0.1% trifluoroacetic acid (TFA; Sigma-Aldrich, Saint Louis, USA). The eluted fractions were pooled together and vacuum-dried ...
-
bioRxiv - Neuroscience 2020Quote: ... from Tocris CGP-55845 ((2S)-3-[[(1S)-1-(3,4-Dichlorophenyl)ethyl]amino-2-hydro xypropyl](phenylmethyl)phosphinic acid hydrochloride) from Sigma-Aldrich
-
bioRxiv - Genomics 2021Quote: ... Expression of the Dam-POI constructs was suppressed by addition of 0.5 mM indole-3-acetic acid (IAA; Sigma, I5148). Lines were tested routinely for mycoplasma.
-
bioRxiv - Cell Biology 2021Quote: ... Auxin-inducible degron (AID)-tagged strains were constructed as described (Snyder et al., 2019) and freshly prepared 10,000x stocks (500 mM) 3-indole acetic acid (IAA) (Sigma Aldrich) dissolved in 100% ethanol were used to treat yeast cultures (YPD ...
-
bioRxiv - Microbiology 2021Quote: ... and human choriocarcinoma/trophoblastic cancer (JEG-3) cells were grown in DMEM supplemented with 10% FBS and MEM Non-essential Amino Acid Solution (Sigma). C6/36 cells were grown at 28°C in a 5% CO2 incubator ...
-
bioRxiv - Microbiology 2021Quote: ... RH TIR1-3FLAG or RH TgPDE-mAID-3HA tachyzoites cultivated in HFFs in D10 medium were treated with 0.5 mM 3-indoleacetic acid (auxin; IAA) (Sigma Aldrich) prepared in 100% ethanol (Pharmco ...
-
bioRxiv - Neuroscience 2022Quote: Larvae were anesthetized in 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) prior to imaging except where noted ...
-
bioRxiv - Bioengineering 2022Quote: ... with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
-
bioRxiv - Biochemistry 2022Quote: ... or TAP-HA-YTM1 RPL3-Flag fusions under control of the respective endogenous promoters were grown in YPD or SDC-Leu medium and as indicated in the figures incubated in the presence of 0.5 mM auxin (3-indoleacetic acid, Sigma-Aldrich) for 120 min prior to harvesting the cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... media was exchanged for 2 mL of culture media supplemented with 500 µM 3-indoleacetic acid (IAA, Millipore Sigma, I2886). Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... Auxin-induced degradation of Ring1b in AID::Ring1b;FUCCI was carried by adding water-dissolved auxin (IAA, Indole-3-acetic acid sodium salt, Sigma) to cell media to a final concentration of 500 µM as described previously 46 ...
-
bioRxiv - Neuroscience 2022Quote: ... All larvae were then anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester at pH = 7.0 (Tricaine; Sigma-Aldrich, A5040), immediately de-yolked in de-yolking buffer (55 mM NaCl ...
-
bioRxiv - Genetics 2023Quote: ... animals of similar age were depleted of AID::GFP expression by culturing for 2 h on NGM plates containing 100 µM auxin (3-indoleacetic acid; Sigma). Directly after depletion ...
-
bioRxiv - Immunology 2023Quote: ... 3 dpf larvae were anesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The corresponding concentration of bacteria was injected into the corresponding part of the zebrafish ...
-
bioRxiv - Molecular Biology 2023Quote: ... TOE1 was depleted in HEK 293T-REx-derived TOE1-degron cells12 by incubation with 600 μM auxin hormone Indole-3-Acetic Acid (IAA, Sigma) for 8 hours ...
-
bioRxiv - Bioengineering 2023Quote: ... 3 µL of 2 M CaCl2 and 5 µL of 10 mg/mL ε-aminocaproic acid (ε-ACA) (Sigma-Aldrich), and 1 µL of 1U/µL Thrombin (MP Biomedicals ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were inoculated in YEP containing 3% lactic acid and treated with a final concentration of 2% galactose (Sigma-Aldrich). For the inducible degradation of the proteins ...
-
bioRxiv - Cell Biology 2023Quote: Lipid overlay assay PI strips were blocked in PBST (0.1% Tween 20 [v/v]) supplemented with 3% (w/v) fatty acid-free BSA (Sigma-Aldrich) overnight at 4°C and then incubated with 0.5 μg/ml GST fusion proteins for 1 h at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... Two length-matched siblings were anesthetized in 0.2 mg/ml ethyl-3-aminobenzoic acid ethyl ester (MESAB, catalog # E10521, Sigma-Aldrich) simultaneously and mounted in 2% low-melting temperature agar ...
-
bioRxiv - Neuroscience 2023Quote: ... Anatomical images were acquired from fish anesthetized with 0.2 mg/ml ethyl-3-aminobenzoic acid ethyl ester (MESAB, catalog # E10521, Sigma-Aldrich). To activate TRPV1-expressing Purkinje cells ...
-
bioRxiv - Genomics 2023Quote: ... Expression of the Dam-POI in the mouse ESC lines was suppressed by addition of 0.5 mM indole-3-acetic acid (IAA; Sigma, I5148).
-
bioRxiv - Neuroscience 2023Quote: ... Larvae were anesthetized with 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) and mounted laterally in 2% low-melting temperature agarose (Thermo Fisher Scientific 16520) ...
-
bioRxiv - Microbiology 2023Quote: ... All intracellular metabolite samples were resuspended in 750μL deuterium oxide containing 1mM 3-(Trimethylsilyl)-1-propanesulfonic acid sodium salt (DSS) (178837, Sigma Aldrich) and 50mM NaIO4 (769517 ...
-
bioRxiv - Immunology 2023Quote: ... The samples were acidified the next day using formic acid to bring pH<3 to stop the trypsin activity and were processed through Zip-Tip using C18 tips (Millipore) to clean-up and concentrate the peptides before going through mass spec analysis.
-
bioRxiv - Immunology 2023Quote: ... The activity of trypsin was inhibited by dropping the pH to 2-3 by the addition of 10% trifluoroacetic acid (TFA, Sigma). The samples were loaded on Evosep tips to separate the digested peptides using nanoflow reversed-phase chromatography with an Evosep One liquid chromatography (LC ...
-
bioRxiv - Cell Biology 2023Quote: Cell lysates were prepared from approximately 3 OD600 units of cells using a trichloroacetic acid (TCA) (Sigma Aldrich, Cat#T9159) lysis method60 ...
-
bioRxiv - Neuroscience 2024Quote: Larvae were anesthetized in 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) prior to confocal imaging except where noted ...
-
bioRxiv - Developmental Biology 2024Quote: ... Auxin treatment was performed by transferring worms to bacteria-seeded NGM plates containing 1 mM auxin (indole-3-acetic acid, Sigma). The preparation of auxin stock solution (400 mM in ethanol ...
-
bioRxiv - Cell Biology 2024Quote: Worms of the indicated genotype were selected as L4s or young adults (24h post L4 stage) and picked onto NGM plates with and without 1 mM indole-3-acetic acid (auxin, Sigma). Exposure to auxin was carried out for the indicated times ...