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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for 3 Chloro phenyl 9H fluoren 9 ylmethoxycarbonylamino acetic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... To visualize protein bands for excising and mass spectrometry processing, gels were stained with Coomassie (40% methanol, 20% acetic acid, and 0.1% Brilliant Blue R (B7920, Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2021Quote: ... The gel was then washed with the fix/wash solution (25% ethanol, 10% acetic acid in water) and stained by 0.1% Alcian blue (Sigma-Aldrich) dissolved in the fix/wash solution for 15 min in the dark ...
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bioRxiv - Developmental Biology 2021Quote: ... The stained sections were rinsed in distilled water and staining outside of perikarya was differentiated for 10 min in 96% EtOH with 0.02% glacial acetic acid (Millipore Sigma). The sections were subsequently dehydrated 2x 5 min in 99% EtOH and 2x 5 min in Xylene prior to coverslip mounting using DPX (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... the pegs were gently rinsed with distilled water and air dried before being submerged in 200 μL of 33% acetic acid (Sigma) for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: Freshly-seeded healthy antral undifferentiated mucosoid cultures were treated every 24 h with 4 µM 5aza (in 0.02 % acetic acid; Sigma-Aldrich). A global decrease in DNA methylation ...
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bioRxiv - Cell Biology 2024Quote: ... Gel binding on the same glass coverslips as for PDMS gels was facilitated by activating the glass surface using a 1:1:14 solution of acetic acid (695092, Sigma)/bind-silane (10700467 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and clozapine (Novartis Pharmaceuticals, Basel, Switzerland) were prepared daily and dissolved in glacial acetic acid and sodium hydroxide (NaOH, Sigma) to reach pH 6 ...
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bioRxiv - Cell Biology 2023Quote: ... 500 µl of 20 mM acetic acid solution containing 50 µg of collagen-I from bovine skin (C4243, Sigma-Aldrich) was added to the substrate supported on a 22×22 mm cover-glass and left for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... The mixture was neutralized with 1 N acetic acid until gas evolution ceased and filtered through Celite 545 (EMD Millipore). The solution was concentrated by rotary evaporation at 50 °C and precipitated in cold diethyl ether (Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μl of supernatant was mixed with 190 μl of deionized water and added to 1 ml of Amido Black Buffer (10% acetic acid, 90% methanol, 0.05% [w/v] Amido Black (Naphtol Blue Black, Sigma N3393)) ...
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bioRxiv - Bioengineering 2024Quote: ... functionalized glass coverslips were immersed in a solution of 25 ml of anhydrous ethanol containing 20 µl of acetic acid and 0.4 mg/ml 4-nitrobenzaldehyde (4NB, Sigma Aldrich) at 50 °C for 3 h ...
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bioRxiv - Plant Biology 2024Quote: ... vortexed and then mixed with 1 ml of Amido Black Buffer (10% acetic acid, 90% methanol, 0.05% [w/v] Amido Black (Napthol Blue Black, Sigma N3393)) by inverting the tubes ...
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bioRxiv - Biochemistry 2024Quote: Collagen IV was reduced by mixing an equal volume of the stock (∼0.3 mg/ml in 0.5 M acetic acid) with 0.25 M TCEP-HCl (Sigma-Aldrich®, C4706) at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... 2:1) were dissolved until homogeneous in a 10 wt% solution containing acetic acid (90% aqueous solution; Sigma Aldrich A6283), citric acid (15 wt% based on gelatin weight ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was freshly prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ∼20 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... was then loaded immediately onto the target and overlaid with 1.2 μl of a matrix consisting of 9H-Pyrido[3,4-B]indole (Norharmane) (Sigma-Aldrich) dissolved in 90:10 (vol/vol ...
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bioRxiv - Synthetic Biology 2020Quote: ... indole-3-propionic acid (order no. 57400) and indole-3-butyric acid (order no. i5386) have been obtained from Sigma-Aldrich. All ligands have been dissolved in 50mM Tris / 300mM NaCl pH8 buffer with if necessary 1% dimethyl-sulfoxide (DMSO) ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed by colorimetry using nitro blue tetrazolium chloride / 5-bromo-4-chloro-3-indolyl-phosphate (NBT-BCIP, Sigma-Aldrich) as a substrate.
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bioRxiv - Molecular Biology 2024Quote: ... the cells were washed 2-3 times with warmed PBS followed by 50 μM 5-chloro-2’-deoxyuridine (CldU, Sigma #C6891) for 20 minutes or 50 μM CldU with 4mM hydroxyurea (HU ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.003% 1-phenyl-2-thiourea (PTU, Sigma, P7629) was used to inhibit pigment formation ...
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bioRxiv - Microbiology 2022Quote: ... 0.003% 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) was added to prevent pigmentation ...
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bioRxiv - Bioengineering 2023Quote: ... and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, Sigma-Aldrich) for GelMA ...
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bioRxiv - Microbiology 2023Quote: ... 0.003% 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) was added to prevent pigmentation ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mM lithium phenyl-2,4,6- trimethylbenzoylphosphinate (LAP; Sigma) and ultraviolet (UV ...
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bioRxiv - Developmental Biology 2024Quote: ... 50× 1-phenyl-2-thiourea (PTU; Sigma-Aldrich) was added to 0.3× Danieau Buffer at a final concentration of 1 × PTU to inhibit the formation of pigmen ...
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bioRxiv - Biochemistry 2021Quote: ... acetic anhydride (>99%) from Sigma Aldrich; HCTU (reagent grade ...
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bioRxiv - Neuroscience 2024Quote: ... followed by being acetylated in 0.1 M Triethanolamine (Sigma #90279)- HCl with acetic anhydride (Sigma #320102). V2R cRNA probes were hybridized with tissue sections overnight at 65 ᵒC in a hybridization solution consisting of 50 % formamide ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 μM 5-chloro-2’-deoxyuridine (CldU; Sigma-Aldrich) and 250 μM 5-iodo-2’-deoxyuridine (IdU ...
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bioRxiv - Cell Biology 2022Quote: ... 25 µM 5-Chloro-2′-deoxyuridine (CldU) (Sigma-Aldrich); 250 µM 5-Iodo-2’-deoxyuridine (IdU ...
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bioRxiv - Cancer Biology 2024Quote: ... 5-Chloro-2′-deoxyuridine (CldU, 25 µM; C6891, Sigma), and 5-Iodo-2′-deoxyuridine (IdU ...
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bioRxiv - Microbiology 2022Quote: ... Peroxidase activity was revealed by incubating slides in 3-amino-9-ethylcarbazole (AEC) (Sigma) for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... and developed using a filtered solution of 0.2mg/mL 3-amino-9-ethylcarbazole (Sigma) and 0.015% H2O2 (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and adenosine 5’-diphosphate sodium salt (ADP) (3 μM) (20398-34-9, Sigma Aldrich) were dissolved in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... 9 μL) and incubated with calf intestinal alkaline phosphatase (51 U, 3 μL, Sigma) for 2 h at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: A 1 w.t.% suspension of insoluble type I bovine dermal collagen (Devro) was prepared in 0.05 M acetic acid solution (Sigma-Aldrich UK). The mixture was left at 4°C to swell for 24 hours and homogenized in a blender at 22,000 rpms for 6 minutes ...
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bioRxiv - Immunology 2021Quote: ... TCA-fixed cells were stained by adding 50 μl of SRB solution 0.4% in 1% glacial acetic acid (Sigma, SRB #S1402) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The pelleted PRP was resuspended in PBS containing prostaglandin E1 (2 μM, Absin) and ethylene diamine tetra acetic acid (EDTA) (5 mM, Sigma-Aldrich) to prevent platelet activation ...
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bioRxiv - Molecular Biology 2022Quote: ... The desalted peptides in the cartridge were eluted using a buffer containing 0.5% acetic acid and 80% acetonitrile (Sigma-Aldrich-Aldrich). The samples were subsequently dried using a speedvac for mass spectrometry analysis.
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bioRxiv - Immunology 2022Quote: ... sections were rinsed in 1% acetic acid followed by dehydration in increasing concentrations of ethanol and xylene and mounting using Entellan (Sigma-Aldrich). Images were acquired using a Leica DM750 microscope (Leica Microsystems ...
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bioRxiv - Molecular Biology 2022Quote: ... at a constant flow rate of 0.8 ml/min and a constant temperature of 22°C with mobile phase A being 0.4 % acetic acid (Sigma-Aldrich, USA) in water and phase B being 20% Methanol (Honeywell ...
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bioRxiv - Bioengineering 2024Quote: ... Rat tail collagen type I solution in 0.6% acetic acid (First Link, UK) was combined with human AB serum (HS; Sigma-Aldrich, H4522), 10x DMEM ...
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bioRxiv - Bioengineering 2023Quote: ... 500 μl of a 20 mM acetic acid solution containing 50 μg of collagen-I from bovine skin (C4243, Sigma-Aldrich) was applied to the substrate ...