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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... and BCIP (5-bromo-4-chloro-3-indolyl-phosphate; Sigma).
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bioRxiv - Neuroscience 2024Quote: D-4-amino-3-isoxazolidone (DCS, C3909 Sigma-Aldrich, UK) was prepared in sterile saline solution (6) ...
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bioRxiv - Molecular Biology 2024Quote: ... native ChIP was performed using an increasing amount of H3K4me3 antibodies (for the Abcam 0, 2, 4, 6, 10μl, for the Diagenode 0, 4, 8, 12μl and for the Millipore 0, 2, 4, 8, 16μl). The number of gene-specific sequences associated with each quantity of antibody was quantified by real-time PCR targeting the gene encoding the 40S subunit (Forward primer ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibodies were washed away with PBS (3x 5 min) whereafter 4’,6-diamidino-2-phenylindole (DAPI, 1:500, Sigma) was added for 10 minutes to localize the cell nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed using 70% Ethanol and re-suspended in PBS containing 1 μg/ml 4’,6-diamino-2-phenylindole (DAPI, Sigma) and analyzed by microscopy ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in 1% normal donkey serum in PBS with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue and cells were incubated in 2-(4-amidinophenyl)-1H -indole-6-carboxamidine (DAPI, D-6564, Sigma, 1:1000). Biotinylated-secondary antibodies were from Vector Labs ...
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bioRxiv - Immunology 2022Quote: ... For cell sorting cell suspensions were stained for viability with 1:3000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) immediately before sorting.
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bioRxiv - Developmental Biology 2022Quote: ... sections were washed in PBS and then incubated for 10 minutes in 1:10000 4’,6-diamidino-2-fenilindol (DAPI; Sigma) for nuclei counterstaining ...
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bioRxiv - Immunology 2022Quote: ... The sections were washed in PBS and incubated 10 min with 4’,6-diamidino-2-phenylindole (DAPI, 1/10,000, Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in medium with 10% fetal calf serum and 1 × 105 cells were cultured for 4 to 6 days with different concentrations of ConA (Sigma) or co-cultured with (2.5 × 104 ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
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bioRxiv - Immunology 2024Quote: ... slides were washed in PBS and incubated in 5 μg mL-1 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Cell Biology 2024Quote: ... samples were washed in 1X PBS and incubated with DAPI (4-6-diamidino-2-phenylindole, Sigma-Aldrich, 1 µg/mL) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst or 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI)) or ExtrAvidinCy3 (Sigma) solution (1:100 in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS and further incubated for 15 min at room temperature with DAPI (4’, 6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibody lasted for 3 h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1x 30 min with 5×SSCT containing 4′,6-diamidino-2-phenylindole (DAPI, 1μg/ml, #62248, ThermoFischer) and/or Phalloidin Atto 565 (1:50, #94072, Sigma Aldrich), and 1x 5min in 5xSCCT ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were washed in PBS and further incubated for 15 min at room temperature with DAPI (4’,6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibody lasted for 3 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... To stabilize beta-catenin and to induce canonical Wnt signaling the cells were treated with 1 μM BIO (6-Bromoindirubin-3′-oxime, SIGMA B1686) for 24 hours before harvesting.
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Cell Biology 2020Quote: ... larvae and adults were incubated in 3 μM and 2.5 μM 4-hydroxytamoxifen (4-OHT, Sigma), respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... 4-nitroquinoline 1-oxide (4-NQO) (Sigma). Treatment conditions are indicated in the corresponding figures ...
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bioRxiv - Microbiology 2021Quote: ... 1 µM 4-hydroxytamoxifen (4-OHT; Sigma) for 1h at 37°C prior to infection and during the course of infection ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated at 4°C for 1-3 days in the presence of Triton X-100 (0.3%, Sigma), 1% normal donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2020Quote: ... Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES) (Sigma, SML0601); an irreversible O-Carnitine palmitoyltransferase-1 (CPT1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Thapsigargin ((3S,3aS,4R,6R,7S,8R)-6-acetoxy-4-(butyryloxy)-3,3a-dihydroxy-3,6,9-trimethyl-8-(((Z)-2-methylbut-2-enoyl)oxy)-2-oxo-2,3,3a,4,5,6,6a,7,8,9b-decahydro-1H-cyclopenta[e]azulen-7-yl octanoate) from Sigma was dissolved in DMSO to a stock concentration of 5 mM ...
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bioRxiv - Bioengineering 2021Quote: ... 3- [(3- Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS, Sigma RES1300C), digitonin (Sigma D141) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/ml 3′,3′-diaminobenzidine tetrahydrochloride hydrate (Sigma), 24 units/ml catalase from bovine liver (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... clone 6-11B-1 ((Sigma-Aldrich, T7451, 1:1000), goat anti-Rat IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... anti-acetylated tubulin (1:5,000, Sigma, 6-11B-1), anti-MAP2 antibody (1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at room temperature for 20 minutes and 50 μL of 1% SDS was used to stop the reaction ...
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bioRxiv - Biochemistry 2020Quote: ... TsFpn was then concentrated to 3-6 mg/ml (Amicon 50 kDa cutoff, Millipore) and loaded onto a size-exclusion column (SRT-3C SEC-300 ...
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bioRxiv - Microbiology 2020Quote: ... 100 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at room temperature and 100 μL of 1% SDS was used to stop the reaction ...
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bioRxiv - Microbiology 2020Quote: ... 50 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at RT and 50 μL of 1% SDS was used to stop the reaction ...
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bioRxiv - Neuroscience 2021Quote: ... and 6-chloro-1,2-benzisoxazol-3(2H)-one (CBIO) were purchased from Sigma Aldrich. Phorbol 12-myristate 13-acetate was purchased from Cayman Chemical ...
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bioRxiv - Immunology 2024Quote: ... 6 uM pargyline and 50 uM (-)-epigallocatechin-3-gallate (EGCG) (all from Sigma Aldrich). For metabolic pathway analysis ...
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bioRxiv - Microbiology 2023Quote: ... 540-69-2] (pH 3, adjusted with formic acid [Sigma Aldrich, 64-18-6]) and solvent B was acetonitrile [Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 3 mM Na+pyruvate (Sigma-Aldrich; St. Louis, MO; CAS No. 113-24-6), 2 mM N-acetyl-L-cysteine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were counter-stained with DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) before mounting in Mowiol.
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bioRxiv - Cell Biology 2020Quote: ... Some sections were additionally labeled with 4′,6-diamidino-2-phenylindole (DAPI, Sigma D9542 ...