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Citations for Millipore Sigma :
5901 - 5950 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...
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bioRxiv - Pathology 2020Quote: ... and 0.1% Triton-X 100 in PBS) and stained overnight at 4° C with antibodies against PRG2 (1:1000 dilution, rabbit; Sigma-Aldrich cat. no. HPA038515), HLA-G (1:10,000 dilution ...
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bioRxiv - Microbiology 2019Quote: ... Blots were probed with the following antibodies diluted 1:5000 in 1X TBS-T containing 5% nonfat dry milk: rabbit anti-FLAG (Sigma-Aldrich Cat# F7425, RRID:AB_439687), mouse anti-FLAG (Sigma-Aldrich Cat# F1804 ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 1 hour at 37⁰C with Duolink® In Situ PLA® Probe Anti-Rabbit PLUS (Sigma Millipore, Cat. DUO92002) and Duolink® In Situ PLA® Probe Anti-Mouse MINUS (Sigma Millipore ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 1 hour at 37⁰C with Duolink® In Situ PLA® Probe Anti-Rabbit PLUS (Sigma Millipore, Cat. DUO92002) and Duolink® In Situ PLA® Probe Anti-Mouse MINUS (Sigma Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... One to three 12 μm sections were chosen randomly from both sides for staining as previously established6 using an anti-CGRP antibody (1:5000, Sigma C8189, anti-rabbit polyclonal), an anti-P2X3 antibody (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and rabbit anti-pSer502-NT5C2 (dilution 1:1000) (Covance) antibodies and β-actin with a mouse monoclonal anti-β-actin antibody (Sigma Aldrich, #A5441). We visualized the immunoblots using an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then blocked for 3 hours in PBST and 5% normal goat or donkey serum at room temperature after which they were incubated in primary antibodies (rabbit anti-GABA 1:500, Sigma-Aldrich Cat# A2052, RRID:AB_477652) for at least 72 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore, C2581; Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T). The intensity of the signal from retained anti-AGO1-N-coil antibody was quantified using Image J and fitted using Nonlinear Least Square (NLS ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... Acetylated histone H4 and H2A were detected by immunoblotting with rabbit anti-acH4 (UBI, Catalog # 06–866, 1:1000 dilution) and anti-acH2A K9 (Millipore Sigma, Catalog # 07-289), respectively.
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies were applied (goat anti-rabbit IgG-Alkaline Phosphatase, A9919, Sigma Aldrich; goat anti-mouse IgG-Alkaline Phosphatase, A4312, Sigma Aldrich; 1:3000) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... After overnight incubation the primary antibodies were washed away and PLA secondary probes were added: Anti-Rabbit MINUS (Sigma Aldrich, #DUO92005, dilution 1:5), Anti-Goat PLUS (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were used for purification of recombinant proteins by 2 h incubation with GST-agarose beads at 4 °C according to manufacturer’s protocol (Sigma Aldrich, St. Louis, USA, G4510). SDS-PAGE electrophoresis was used to check level of expression and purity of purification.
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Genomics 2024Quote: ... The reaction products were analyzed on 8% PAGE-SDS gels with silver staining or Western blotting for PAR using a recombinant anti-poly-ADP-ribose binding reagent (Millipore, Cat# MABE1031; RRID: AB_2665467).
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Cell Biology 2024Quote: ... or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Cell Biology 2019Quote: ... and immunoblotted with the indicated antibodies followed by HRP-conjugated goat anti–mouse IgG or anti–rabbit IgG or rabbit anti–chicken IgY (Sigma-Aldrich) and Super Signal West Pico Chemiluminescent Substrate (PI34080 ...
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bioRxiv - Cell Biology 2019Quote: ... A polyclonal rabbit anti-AGPAT (SAB2700797) and a monoacylglycerol lipase An MAGL rabbit polyclonal antibody (Cat. 011994) were purchased from Sigma-Aldrich. A rabbit anti-ATGL (mAb ab207799 ...
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bioRxiv - Cancer Biology 2019Quote: ... The RBM proteins were identified with the primary rabbit anti-RBM10 or RBM5 antibody and the following secondary anti-rabbit IgG conjugated with Horse radish peroxidase (A6154; Sigma-Aldrich) or conjugated with alkaline phasphatase (T2180;Applied Biosystems).
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bioRxiv - Cell Biology 2021Quote: ... 2013), rabbit anti-Mad2 (Heinrich et al., 2013), rabbit anti-Mad3 (Heinrich et al., 2013) or mouse anti-tubulin (Sigma, T5168). Secondary antibodies were either anti-mouse or anti-rabbit conjugated to HRP (Dianova ...
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bioRxiv - Microbiology 2019Quote: ... The TopA protein was subsequently detected using primary rabbit polyclonal anti-TopA antibodies and mouse secondary anti-rabbit IgG antibodies conjugated with alkaline phosphatase (Sigma Aldrich), according to the procedure described previously (Szafran et al. ...
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bioRxiv - Microbiology 2020Quote: ... or anti-gL (rabbit polyclonal) antibodies and detected using anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase (Sigma-Aldrich) and Pierce ECL-Western blotting substrate (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... the plates were then washed with PBST and incubated with the detecting antibody: alkaline phosphatase (AP)-conjugated goat anti rabbit for rabbit serum (Sigma-A8025), horseradish peroxidase (HRP)-conjugated anti-M13 antibody (GE healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1hr and probed overnight with primary antibodies (Rabbit anti-SDMA, CST13222; mouse anti-vinculin, Sigma, RABBIT anti-PRMT5 (Abcam Ab31751). Blots were washed 3X with 1% TBST buffer and probed with secondary antibodies 680RD goat anti-Mouse and 800CW goat anti-Rabbit (LI-COR ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualised using the Licor Odyssey CLx system with Licor secondary antibody (IRDye 800 CW anti-rabbit/mouse) or anti-mouse IgG (whole molecule) (A4416) or anti-rabbit IgG (Sigma-Aldrich) conjugated to horseradish peroxidase.
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-PDGFRβ (eBiosciences 14-1402-81), rabbit anti-AQP3 (Alomone Labs, AQP-003, after antigen retrieval) rabbit anti-Runx1 (Sigma, HPA004176), rabbit anti-IRF9 (Cell Signalling ...
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bioRxiv - Biochemistry 2023Quote: ... and detected by standard western blotting procedures using rabbit anti-sera against IbpA (Eurofin) as primary antibody and HRP-conjugated anti-rabbit IgG as secondary antibody (Sigma-Aldrich). For IbpACn and IbpAVh detection ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation of chromatin preparations was performed using rabbit anti-CLAMP antibody (Rieder et al., 2017) and a polyclonal rabbit IgG (Millipore-Sigma, 12-370). qPCR was performed as described in (Urban et al. ...
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bioRxiv - Microbiology 2021Quote: ... Total cell lysate was also subjected to immunoblotting with anti-T7e rabbit antibodies and anti-HA rabbit polyclonal antibodies (Sigma Aldrich, H6908) to evaluate the expression levels of Env-T7es and HA-MARCH8 ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated with specific primary antibodies (mouse anti-Esrrb, Perseus PP-H6705-00; rabbit anti-Klf4, Santa Cruz, sc-20691; rabbit anti-Tfcp2l1, AbCam, ab123354; anti-βactin, Sigma, A3854). Blots were incubated with horseradish peroxidase (HRP)-coupled sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was subsequentially incubated with a horseradish peroxidase (HRP)-conjugated α-mouse whole IgG antibody produced in rabbit (Sigma-Aldrich, CAT. #A9044; 1:80000). Protein-HRP chemiluminescence was detected using Chemidoc XRS1 System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4) with 1% bovine serum albumin and then incubated overnight in a purified antiserum against TH generated in rabbits (Sigma-Aldrich, Søborg, Denmark; cat#AB152) diluted 1:500 in PBS + 0.1% Triton-X overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... in PBS-T (0.01% Triton-X 100) and then incubated overnight in rabbit anti-c-Fos primary antibody (1:5000; EMD millipore; Cat. No: ABE457; Lot: 2987437) or the rabbit anti-mCherry primary antibody (1:200 ...
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bioRxiv - Biochemistry 2021Quote: ... primary antibodies followed by anti-mouse (#A4416) and anti-rabbit (#A0545) secondary antibodies conjugated to horseradish peroxidase (both are from Millipore-Sigma and used at 1:10,000). WesternBright Sirius chemiluminescent horseradish peroxidase substrate (Advansta ...
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bioRxiv - Bioengineering 2023Quote: ... In a subset of IHC experiments we performed co-immunostaning with additional cell type marker antibodies including the following: rabbit anti-GLUT1 (Millipore catalog #07-1401, 1:1000), rabbit anti-Olig2 (Abcam catalog #AB9610 ...
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bioRxiv - Plant Biology 2020Quote: ... Prior to labelling the apoplastic fluid was incubated for 30 min with different concentrations of recombinant LpCys1 or chicken egg white cystatin (CEWC, Sigma-Aldrich, St. louis, Mississippi, USA) and Tris-HCl (24 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the chamber was rinsed three times with T50 to remove non-specific interactions and a protocatechuic acid/recombinant protocatechuate-3,4-dioxygenase (Sigma Aldrich and Oriental Yeast Co., respectfully) (PCA/rPCD ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-rabbit lgG (A6154 Sigma and 7074 Cell Signaling) and anti-mouse lgG (7076 Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... We purchased rabbit polyclonal antibodies to FLAG (Sigma-Aldrich) and Erk1/2 (Cell Signaling) ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal antibody against γ-tubulin (Sigma, Cat.# T3559), as well as rat monoclonal antibody against α-tubulin (Chemicon ...
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bioRxiv - Genetics 2021Quote: ... rabbit anti-H3K9ac (EMD Millipore; catalog no. 06-942), mouse anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... rabbit anti-H4K16ac (EMD Millipore; catalog no. 07-329), rabbit anti-H3 (AbCam ...
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bioRxiv - Microbiology 2019Quote: ... or anti-rabbit IgG (Millipore, 12-370, 10 µg) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies were anti-Six1 (rabbit polyclonal HPA001893, Sigma) and anti-beta-Tubulin (mouse monoclonal hybridoma clone E7 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or 1ug of normal rabbit IgG antibody (Sigma Aldrich). Input and immune-precipitated samples were purified using a Qiagen PCR purification kit ...