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Citations for Millipore Sigma :
5751 - 5800 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... were subjected to immunoblot analysis with specific primary antibodies (Table 1) and secondary antibodies (anti-mouse/rabbit IgG coupled to horseradish peroxidase; Sigma Aldrich; 1:5000 dilution in blocking buffer). Detection of chemi-luminescent signal was performed with Clarity ECL Western Blotting substrate (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were incubated during 48 h at 4°C in a blocking solution containing the primary antibody against TH (rabbit anti-TH, Millipore AB152, lot 2795024 and 3114503 (1:1500) RRID ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were dissected out and washed extensively in PBS before blocking in staining buffer (10% normal goat serum, Sigma-Aldrich, and 2% Triton X-100 in PBS) for half an hour ...
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bioRxiv - Cell Biology 2019Quote: ... the slides with attached intact axonemes were treated overnight at 4°C with blocking buffer containing the diluted primary antibody (mouse anti-acetylated tubulin, Sigma-Aldrich, clone 6-11B-1, 1:1000). On the next day ...
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bioRxiv - Cancer Biology 2020Quote: ... The equivalent of 5 mg of protein was further enriched for phosphorylated tyrosine peptides by overnight immunoprecipitation (pY-IP) using a cocktail of anti-phosphotyrosine antibodies (4G10, Millipore; PT-66, Sigma;p-Tyr-100, Cell Signaling Technology).
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bioRxiv - Biochemistry 2021Quote: ... and purified using DDDDK-tagged protein purification gel (MBL, Japan) followed by elution with 0.1 mg/ml FLAG Peptide (Sigma-Aldrich Co., St. Louis, Missouri, the US). To remove the flag peptide ...
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bioRxiv - Cell Biology 2021Quote: ... protein was eluted by incubating 1 CV of elution buffer (10 mM Tris, 200 mM NaCl, 1 mM DTT, 1 mM EGTA, 0.2 mg/mL FLAG peptide (Sigma-Aldrich, St. Louis, MO, US, SAB1306078-400UL), pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated with Amicon® 3 kDa MWCO concentrator (Millipore, Burlington, MA) and injected into a 10/300 Superdex 75 size-exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with protease inhibitor cocaktail (P8340) and phosphatase inhibitor cocktail 3(Milipore Sigma, P0044)) ...
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bioRxiv - Microbiology 2020Quote: LB-agarose pads were prepared by boiling 1.5% or 3% ultrapure agarose (Sigma Aldrich) in LB ...
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bioRxiv - Microbiology 2020Quote: LB-agarose pads were prepared by boiling 1.5% or 3% ultrapure agarose (Sigma Aldrich) in LB ...
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bioRxiv - Microbiology 2020Quote: ... 13 mM glycine and 3 μM thiamine (all compounds from Merck Millipore, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2021Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased by SIGMA Aldrich and resuspended at 5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... the silicon nitride cantilevers were functionalized with (3-glycidyloxypropyl)trimethoxysilane (98%, Sigma Aldrich, 440167) at 80 °C for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... This neutralized 3-BrPA solution was then sterilized with a 0.22 µm filter (Millipore), and used immediately.
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bioRxiv - Immunology 2021Quote: ... Larvae were anesthetised with 2.5% (v/v) ethyl-3-aminobenzoate methanesulfonate (tricaine) (Sigma, E10521), wounded posterior to the notochord using a sterile scalpel and kept in a 28°C incubator to recover as previously described (27) ...
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bioRxiv - Neuroscience 2020Quote: ... 2000) containing 0.3% ethyl 3-aminobenzoate methanesulfonate (Tricane; MS-222(Cat E10521, Sigma-Aldrich)) ...
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bioRxiv - Developmental Biology 2021Quote: Adult mice (3-7.5 months) were treated with Angiotensin II (cat. #A6402, Sigma-Aldrich) for two weeks ...
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bioRxiv - Microbiology 2019Quote: ... Kasumi-3 cells (ATCC® CRL-2725) were cultured in RPMI-1640 media (Sigma) supplemented with 20% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1-3 units of Jump Start AccuTaq LA DNA polymerase mix (Sigma-Aldrich). Thermal cycles of amplification were carried out in a Personal Eppendorf Mastercycler (Eppendorf ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP, Sigma Israel, B8503). In the foxd3/Rspo1 ISH experiment ...
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bioRxiv - Cell Biology 2022Quote: ... immediately followed by a coating of 3- (trimethoxysilyl)propyl methacrylate (TMS) (Sigma-Aldrich, USA) to methacrylate the glass surfaces (Guvendiren et al. ...
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bioRxiv - Neuroscience 2021Quote: Tadpoles were briefly anesthetized with 0.1% MS-222 (ethyl 3-aminobenzoate methanesulfonate, Sigma-Aldrich), and subsequently immobilized by immersion in a 10 μM α-bungarotoxin (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide] dye solution (Sigma-Aldrich, USA) (5 mg MTT in 1 ml PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 500 μl PBS (PBST) containing 3% normal donkey serum (S30-100ML, Millipore, Hertfordshire, UK) at room temperature (RT ...
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bioRxiv - Physiology 2021Quote: ... rAAVs were washed 3 times with PBS using Amicon Ultra Centrifugal Filter Units (Millipore) and resuspended in PBS + 0.001% Pluronic F68 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed 3 times in PBS and fixed in 4% paraformaldehyde (Sigma Alrich) PBS for 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: Bonner’s Salts was used to make the solutions of 3 μM Ionomycin (Sigma, I0634) and DMSO carrier control ...
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bioRxiv - Cell Biology 2022Quote: ... Tryptophan and Histidine and containing 50 mM of 3AT (3-amino-1,2,3-triazole, Sigma). Picture of the plates were taken using the Fusion FX6 EDGE Imaging System (Vilber ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... then 85℃ for 10 minutes and transfected in a 3:1 polyethylenimine (PEI, Sigma):DNA ratio into 70% confluent HEK 293A cells (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Neuroscience 2020Quote: ... blocked for 3 hours in PBS containing 10 % normal goat serum (NGS) (Sigma-Aldrich), before incubation with anti-GluA2 (RRID:AB_10761960 ...
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bioRxiv - Microbiology 2019Quote: ... before being concentrated using Amicon Ultra-2 centrifugal 3 kDa filter units (Millipore Sigma). For Western blot analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 mM 8-bromoadenosine 3□,5□-cyclic monophosphate (Sigma-Aldrich, Saint Louis, MO, USA), 1 μM medroxyprogesterone acetate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... 2’-O-dibutyryladenosine 3’,5’-cyclic monophosphate sodium salt (db-cAMP; 1 mM; Sigma). Media was replaced every 2-3 days for a minimum of 15 days ...
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bioRxiv - Biochemistry 2019Quote: ... and csm5 were cloned into pACYCDuet-1 (5’-NcoI, 3’-XhoI; Novagen, Merck Millipore), csm4 was cloned into pEHisTEV (5’-NcoI ...
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bioRxiv - Biochemistry 2019Quote: ... and csm5 were cloned into pACYCDuet-1 (5’-NcoI, 3’-XhoI; Novagen, Merck Millipore), csm4 was cloned into pEHisTEV (5’-NcoI ...
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bioRxiv - Cell Biology 2019Quote: ... concentrated with an Amicon Ultra-15 centrifugal 3 kDa MWCO filter (Millipore Cat# UFC900324), endotoxin purified using a Pierce high capacity endotoxin removal spin column (ThermoFisher Cat# 88274) ...
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bioRxiv - Molecular Biology 2019Quote: ... then incubated in 3 mL of 0.025% trypsin-EDTA solution (Sigma Cat No. T4049) for 2-5 min at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... using 3 g/l Phytagel as solidifying agent (Sigma-Aldrich®, St. Louis, USA.) and then incubated under 16L:8D photoperiod at 27 °C.
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bioRxiv - Systems Biology 2019Quote: ... Sodium bicarbonate and sodium borohydride were purchased from EMD Millipore (#SX0320-1, #SX0380-3). mPEG-Succinimidyl Valerate (MPEG-SVA-5000-5g ...
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bioRxiv - Cell Biology 2019Quote: ... 3-isobutyl-1-methylxanthine (I5879) and 2’,5’-dideoxyadenosine (D7408) were purchased from Sigma. Carvedilol (15418 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were incubated overnight and then selected by puromycin (3 μg/mL, Sigma-Aldrich). Cells were passaged and cultured under puromycin selection for at least 5 days before flow cytometry analysis using an LSRFortessa X20 instrument (BD Biosciences).
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bioRxiv - Biophysics 2019Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647N (Atto 647N DOPE) (Sigma) was incorporated into all lipid bilayers for visualization ...
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bioRxiv - Cancer Biology 2019Quote: ... Endogenous peroxidase activity was blocked by incubating the slides in 3% wv H2O2 (Sigma). The blocking buffer contains 0.2% goat serum ...
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Obesity alters mobility and adult neurogenesis, but not hippocampal dependent learning in ob/ob micebioRxiv - Neuroscience 2019Quote: ... the solution containing the primary rabbit anti-active Caspase 3 antibody (Merck Millipore, Germany) was applied 1:100 in blocking serum (3 % NGS + 0.1 % Triton X-100 in PBS ...
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bioRxiv - Microbiology 2019Quote: ... 3-(N-Morpholino) propanesulfonic acid (MOPS) was obtained from Sigma Aldrich (St. Louis, MO). YPD broth medium and YPD agar were obtained from Becton ...