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Citations for Millipore Sigma :
5701 - 5750 of 10000+ citations for Recombinant Human PDCD5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Dialyzed protein was concentrated using an Amicon Ultra concentrator (30 kDa MWCO, Millipore) and aliquoted into 100 μl amounts and stored at −80 °C.
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-Golgi 58K protein (formimidoyltransferase-cyclodeaminase) mAb (G2404) was from Sigma-Aldrich and used at a dilution of 1:50∼100 ...
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bioRxiv - Cell Biology 2023Quote: Proteins separated with SDS-PAGE were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). Membranes were blocked with 5% skim milk in Tris-buffered saline with 0.05% Tween 20 for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... and rabbit anti-glial-fibrillary acidic protein (GFAP) (Millipore Sigma, AB5804, 1:500) on shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were transferred onto nitrocellulose membranes (0.45 µm pore size; Sigma, cat. # GE10600012) for 2.5 h at 400 mA constant ...
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bioRxiv - Neuroscience 2022Quote: ... protein was further concentrated using Amicon Ultra-0.5 mL centrifugal filters (Millipore Sigma) prior to use ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated with first and secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Protein gels were transferred for 60 min to 0.2µm PVDF membranes (ISEQ00010, Sigma) using the semi-dry Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... and protein bands were visualised via coating the membrane in tetramethylbenzidine (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... and precipitated proteins competitively eluted with Flag peptide (150 ng/µl; F4799, Sigma). Lysates were denatured in SDS-PAGE buffer (4x Lämmli buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were concentrated and the protein concentration measured by BCA assay kit (Sigma). ATPase assay ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto PVDF membranes (Immobilon-FL, 0.45 μm pore size, Millipore) following standard procedures ...
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bioRxiv - Developmental Biology 2023Quote: ... for 1 hr at 115 V before transferring to protein membrane (IPV00010, Millipore) at 50 V for 2 hr ...
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bioRxiv - Developmental Biology 2023Quote: Protein was extracted from maternal liver using RIPA buffer (Sigma-Aldrich Corp., R0278). Protein concentration was determined by a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). After ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantifications were normalized with the β-actin signal (A3854; Sigma-Aldrich®).
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bioRxiv - Neuroscience 2023Quote: ... followed by rotating incubation with 20 µl of protein G-Agarose beads (Sigma) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to nitrocellulose membranes (Millipore). Membranes were probed with a 1:1000 dilution of anti-SARS-CoV-2 S mouse monoclonal primary antibody (1A9 ...
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bioRxiv - Biochemistry 2023Quote: ... and aldehyde concertation was determined using Protein Carbonyl Content Assay Kit (Sigma-Aldrich). Aldehyde concentration was determined to be 4 mol aldehyde per mol protein ...
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bioRxiv - Molecular Biology 2023Quote: ... to which Magna ChIP™ Protein A+G Magnetic Beads (Millipore, 16-663) were added for 2hour at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore). Three major bands on the membrane were cut and subjected to N-terminal Edman sequencing by Hokkaido System Science Co. ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was determined using a bicinchoninic acid kit (BCA, Sigma, BCA1-1KT) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... Fractions containing protein were concentrated in 50-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2023Quote: ... fractions containing protein were concentrated in 10-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was concentrated in a 50 kDa cut-off Vivaspin (Millipore) and run on a Superose 6 size exclusion column (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 µM Rho-associated protein kinase (ROCK) inhibitor (Merck Millipore, SCM-075). On day 3 ...
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bioRxiv - Biophysics 2023Quote: ... the protein was digested online by a pepsin/FPXIII (Sigma-Aldrich P6887/P2143) mixed protease column maintained at 20 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were then concentrated using Amicon Ultra centrifugal filters (30k MWCO, Millipore). The protein concentration of eluates were estimated with a Bradford assay (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... proteins from the gel were transferred onto a PVDF membrane (Millipore, cat#IPVH00010). The membrane was subsequently blocked and incubated with primary and secondary antibodies ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Cancer Biology 2023Quote: ... the protein was concentrated using an 10K MWCO centrifugal concentrator (Amicon-15, Millipore) to 20 mg/mL and purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Proteins were then precipitated upon addition of 50% trichloroacetic acid (TCA, Sigma-Aldrich) to a final concentration of 5% ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins coupled to streptavidin-coated magnetic beads were reduced with dithiothreitol (Sigma-Aldrich) and alkylated with iodoacetamide ...
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bioRxiv - Physiology 2023Quote: ... Proteins were then concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... purified WT or ΔFN2 proteins were incubated with rat tail collagen (EMD Millipore) and then co-IP was carried out as described above.
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bioRxiv - Plant Biology 2023Quote: ... The protein eluent was concentrated using a 100-kDa cut-off Centricon (Millipore) and subsequently loaded onto a Superose 6 Increase 5/150 GL column (Cytiva ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Immunology 2023Quote: ... Total protein concentration was determined using the QuantiPro BCA assay kit (Sigma-Aldrich) by first interpolating the calibration curve using BSA standard solution (0.5 – 30 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... aliquots of protein were digested twice (overnight and 4h) with trypsin (Sigma-Aldrich) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were concentrated by centrifugal filters (Amicon Ultra 10K, Merck Millipore Ltd.). Protein concentrations were determined by a Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein was concentrated by centrifugal ultrafiltration using Amicon Ultra 30 K (Millipore) and quantified spectrophotometrically at 280 nm (mass extinction coefficient E 1% ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were buffer-exchanged against 100 mM ammonium acetate buffer pH 7.4 (Sigma) using Amicon centrifugal filters (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtered using an Ultrafree 0.22 μm centrifugal filter (Merck Millipore) to remove any aggregation ...
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bioRxiv - Cell Biology 2023Quote: ... protein was extracted from SC cells using lysis buffer (CelLyticTM M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... proteins were transferred onto nitrocellulose membranes and stained using Ponceau S (Sigma Aldrich). After destaining ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins in samples were detected using Immobilon Western Chemiluminescent HRP Substrate (Sigma-Aldrich) and image acquisition was performed on a G-Box (Ozyme) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein estimation was performed using BCA method as per the manufacturing protocol (Sigma). 20µg of protein from each sample prepared in 1x protein loading dye was used for polyacrylamide gel electrophoresis (Biorad) ...