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Citations for Millipore Sigma :
5701 - 5750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Sections were sequentially washed with 0.1% Triton X-100 (Sigma, T-9284) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... blocked in 10% Normal Goat Serum (EMD Millipore, S26), 2% Bovine Serum Albumin (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... and glycerol (Sigma, G6279-500) for long-term storage at -20°C.
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bioRxiv - Genetics 2024Quote: ... 50 μM 2-mercaptoethanol (Sigma-Aldrich, #M6250-100ML), and 20 mM HEPES (pH 7.4 ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 1% diethyl pyrocarbonate (Sigma), 0.1% SuperaseIn RNase Inhibitor (Thermo) ...
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bioRxiv - Genetics 2024Quote: ... K562-Bcl2 and K562-PKcs-/- cells were selected with 5 μg/ml puromycin (Sigma-Aldrich, #P8833-100MG), and K562-iCas9 cells were selected with 200 μg/ml G418 (BioVision ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1% Triton X-100 (Sigma, T-9284) in PBS for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... and Alcian blue 8GX (Sigma, A-5268) with a pH adjusted to 2.5 and filtration after overnight stirring ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected by ECL chemiluminescent detection system (Millipore) in Fusion FX (Vilber Lourmat).
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bioRxiv - Molecular Biology 2024Quote: ... For liquid chromatography a SeQuant ZIC-pHILIC 5 μm Polymeric column 150 * 2.1 mm (Merck Millipore, Darmstadt, Germany) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041, Sigma) dissolved in TBST (0.05% tween) ...
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bioRxiv - Molecular Biology 2024Quote: ... Tubulin (T5168, Sigma-Aldrich); RMND5A (custom ...
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bioRxiv - Molecular Biology 2024Quote: ... After staining with secondary antibodies (conjugated with either Alexa Fluor 488 or 594; Millipore) and Hoechst 33342 (BD Biosciences) ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 9 (M5162, Sigma-Aldrich), then resuspended in 20 mM DMP (Dimethyl pimelimidate dihydrochloride ...
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bioRxiv - Molecular Biology 2024Quote: ... HA (HA-7, H3663 Sigma-Aldrich); FLAG (M2 ...
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bioRxiv - Molecular Biology 2024Quote: ... to a final concentration of 0.1 U/μl at room temperature for 15 minutes and immunoprecipitated with anti-Flag magnetic beads (Millipore Sigma) at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended in 20 mM DMP (Dimethyl pimelimidate dihydrochloride, D8388, Sigma-Aldrich) in 100 mM sodium borate ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein expression was induced by 1 mM isopropyl 1-thio-β-D-galactopyranoside (IPTG, Sigma) at 16°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 9 (M5162, Sigma-Aldrich), then resuspended in 20 mM DMP (Dimethyl pimelimidate dihydrochloride ...
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bioRxiv - Molecular Biology 2024Quote: ... pre-cleared and rotated overnight at 4°C with FLAG (M2, F1804, Sigma-Aldrich) or HA (HA-7 ...
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bioRxiv - Molecular Biology 2024Quote: ... Point mutations were generated via site directed mutagenesis with KOD polymerase (71086-3, Novagen) followed by Dpn1 digest (R0176 ...
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bioRxiv - Molecular Biology 2024Quote: ... VFLIP for narrow window selection was buffer exchanged into 200 mM ammonium acetate using 50 kDa molecular weight cut-off (MWCO) Amicon® Ultra Centrifugal Filter (Sigma Aldrich). Samples were run at approximately 1 µM complex concentration for native mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: ... protein was loaded onto a 10% or 8% SDS-PAGE gel and was transferred to Immobilon-FL PVDF membrane (IPFL00005, Millipore). The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then exposed to medium containing the indicated concentration of MMS (Millipore Sigma) for 24 hours at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... For shRNA-mediated knockdown, all TRC pLKO.1 vectors (targeting the YTH domain-containing proteins, THOC1, and THOC2) were purchased from Sigma. The TRIPZ doxycycline (Dox)-inducible lentiviral YTHDC1 shRNA vector is purchased from Dharmacon.
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with 0.5 µg/mL doxycycline (Sigma) for at least 72 hours.
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended in 20 mM DMP (Dimethyl pimelimidate dihydrochloride, D8388, Sigma-Aldrich) in 100 mM sodium Borate ...
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bioRxiv - Molecular Biology 2024Quote: ... 250 nM Torin1 (475991, Millipore Sigma), and 10 µM PFI-7N or PFI-7 (Structural Genomics Consortium ...
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bioRxiv - Molecular Biology 2024Quote: Samples were digested to nucleosides at 37°C overnight with Nuclease S1 from Aspergillus oryzae (Millipore Sigma), followed by dephosphorylation with FastAP alkaline phosphatase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and 75 µg/mL digitonin (D141, Millipore Sigma). After a 10 minute incubation on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.3% Triton X-100 (Sigma T8787), and 2% bovine serum albumin (Gibco 15260037 ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μg/mL Polybrene (Sigma-Aldrich), and 8 μL of GP-transfect-Mate transfection reagent (GenePharma ...
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bioRxiv - Molecular Biology 2024Quote: ... rabbit anti-horse IgG conjugated to horseradish peroxidase (Sigma, A6917-1mL) was diluted 1:2000 in PBS and 100 µL added per well ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... and mounted using FluorSave™ mounting media (Sigma Aldrich, St. Louis, MO).
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bioRxiv - Molecular Biology 2024Quote: ... the purified DNA was dropped onto a positively charged nylon memberane (Millipore Sigma, 11209299001) and the membrane was crosslinked by exposing with 0.12 J/cm2 of 254 nm UV ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... Cells were passed through a 40μM cell strainer (Sigma) to remove debris and clumps ...
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bioRxiv - Molecular Biology 2024Quote: ... The US) equipped with a Supelco NukolTM 25357 column (30m x 530 µm x 1.0 µm; Sigma-Aldrich, St. Louis, MO, The US), hydrogen as a carrier gas ...
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bioRxiv - Molecular Biology 2024Quote: Cultured HAEC cells were stained for SA-β-galactosidase according to the manufacturer’s protocol (Merck Millipore). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... was dissolved in 100 mL of citrate buffer (50 mM, pH 4) containing 25 μL of H2O2 (Sigma, H1009). 100 µL of the resulting buffer was added per well and allowed to develop for 15 minutes in the absence of light ...
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bioRxiv - Molecular Biology 2024Quote: ... were coated with 100 ng/well of each venom diluted in carbonate-bicarbonate buffer (50 mM, pH 9.6, Sigma, C3041) and incubated for 1 hour at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... before the addition of 200 µL of chloroform (Sigma, 288306). Samples were then centrifuged at 12,000 x g for 15 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified RNAs were subjected to library preparation using a SeqPlex RNA Amplification Kit (Millipore Sigma) and sequenced on an Illumina NovaSeq 6000 in the Genome Technology Access Center at Washington University.
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bioRxiv - Molecular Biology 2024Quote: ... Thiazole Blue tetrazolium bromide (MTT; M5655, Sigma Aldrich) solution (120 μL at 0.83 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... and 8 μL of GP-transfect-Mate transfection reagent (GenePharma) were incubated to prepare a 30 μL of 20% F-127 pluronic gel (Sigma-Aldrich) at 4℃ for 2 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM PMSF) and immunoprecipitated by anti-Flag agarose (A2220, Sigma-Aldrich) for 3 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... A 100 mg tablet of 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma A9941) was dissolved in 100 mL of citrate buffer (50 mM ...
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bioRxiv - Genetics 2024Quote: ... or Flag M2 magnetic beads (Sigma M8823) at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 2x saline-sodium citrate (SSC; #S6639, Sigma-Aldrich), 1 µg/µl BSA (#R396A ...
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bioRxiv - Molecular Biology 2024Quote: ... 750 µM IAA sodium salt (Sigma-Aldrich) was supplemented to cell culture medium for 6 hr before cell harvest ...
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bioRxiv - Molecular Biology 2024Quote: ... The probes were co-hybridized in hybridization buffer (20% dextran sulfate (#42867-5G, Sigma), 2x saline-sodium citrate (SSC ...