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Citations for Millipore Sigma :
5651 - 5700 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... aliquots of protein were digested twice (overnight and 4h) with trypsin (Sigma-Aldrich) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Total protein concentration was determined using the QuantiPro BCA assay kit (Sigma-Aldrich) by first interpolating the calibration curve using BSA standard solution (0.5 – 30 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysate was precleared with Protein G agarose beads (EMD Millipore 16-266) for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125 ...
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bioRxiv - Pathology 2023Quote: AAA tissue protein was extracted using RIPA buffer with proteinase inhibitor (Sigma #MCL1). Protein quantification was done by Bradford assay ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins on the gel were transferred to a PVDF membrane (Millipore IPFL 00010), which was first blocked with 10 % milk ...
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bioRxiv - Bioengineering 2022Quote: ... Protein bands were visualized by ECL detection kit (EMD Millipore, Burlington, MA, USA) and images were acquired using the ChemiDoc MP image system (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were precipitated from sucrose fractions using trichloroacetic acid (TCA; Sigma-Aldrich, T6399). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluates were concentrated with Microcon PL-30 (30,000 NMWL) protein concentrators (Millipore Sigma) and loaded onto 1 cm diameter ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then transferred onto hydrophobic polyvinylidene difluoride membranes (PVDF, 0.45 µm, Millipore), followed by blocking in milk buffer (Tris-buffered saline [TBS] [0.5 M Tris pH 8.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... and separated proteins were then transferred to PVDF membranes (polyvinylidene difluoride, Merck Millipore). After treatments with a blocking reagent (Blocking One solution ...
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bioRxiv - Microbiology 2023Quote: ... Secreted proteins were precipitated by addition of 10 % trichloroacetic acid (TCA, Sigma-Aldrich) followed by centrifugation for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Flag-tagged proteins were bound to anti-FLAG M2 affinity gel (Sigma-Aldrich) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were eluted and concentrated with Amicon Centrifugal Filter Units 10K (Millipore) to remove excessive glutathione.
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with biotin-conjugated hyaluronic acid binding protein (HABP) (Sigma 385911), followed by streptavidin conjugated to AlexaFluor-488 (ThermoFisher S11223) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore). Three major bands on the membrane were cut and subjected to N-terminal Edman sequencing by Hokkaido System Science Co. ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was concentrated in a 50 kDa cut-off Vivaspin (Millipore) and run on a Superose 6 size exclusion column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Cell proteins were isolated by the SDS-PAGE blotted on PVDF membranes (Millipore). And then blocked with 5% fat-free dry ilk at RT for 1 h ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were separated by SDS-PAGE and transferred onto PVDF membrane (Millipore) by semi-dry blotting using 1x Tris-glycine buffer (192 mM glycine ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was concentrated with a 3.5 kDa MWCO Amicon ultracentrifuge filter device (Millipore). After filtering the protein through a syringe filter (0.45 µm) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was concentrated with a 3.5 kDa MWCO Amicon ultracentrifuge filter device (Millipore) to a concentration of 5 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to nitrocellulose or PVDF membranes (Immobilon-P membrane, Millipore; Biorad). and were blocked in 5% milk or 2.5% BSA in TBS-T for 1 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared using 1X RIPA buffer containing protease (#539131, Merck Millipore) and phosphatase (#524629 ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of protein were transferred on a PVDF membrane (Merk Millipore, IPVH00010). Blocked at 5% fat-free bovine milk with PBS and 0.1% Tween-20 (Beyotime ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified mini-HA protein was concentrated by Amicon spin filter (Millipore Sigma) and filtered by 0.22 µm centrifuge Tube Filters (Costar) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (ECL, EMD Millipore, #WBKLS0500) and the BioRad ChemiDoc MP (#12003153 ...
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bioRxiv - Neuroscience 2023Quote: ... The separated proteins were transferred onto a PVDF membrane (Millipore Sigma, 0.45μm, IPFL00010) by wet transfer (overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration in supernatants was analyzed with the Bradford reagent (Sigma-Aldrich, USA) using bovine serum albumin in lysis buffer as a standard solution ...
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bioRxiv - Molecular Biology 2023Quote: ... protein-bound beads were resuspended in 50mM TEAB pH 8 (Sigma-Aldrich, T7408) containing 1 μg trypsin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
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bioRxiv - Immunology 2023Quote: ... The protein fractions with high purity were concentrated with a centrifugal concentrator (Millipore). TAB1 (468-504 ...
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bioRxiv - Molecular Biology 2023Quote: Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore) utilizing a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... the proteins were transferred to the polyvinylidene fluoride (PVDF) membrane (0.45 μm, Millipore) and immunoblotted using the primary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Pre-prepared Protein A Dynabeads (incubated with 5 μg of S9.6 antibody (Millipore) in 1X PBS plus 0.5% BSA overnight at 4°C) ...
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bioRxiv - Neuroscience 2023Quote: ... after which the interesting proteins were competitively eluted using 3XFLAG peptide (Sigma, F4799). The eluent was then incubated overnight at 4°C with Ni-NTA beads (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Total protein content was quantified using the Bradford dye-binding method (Sigma, #B6916). A total of 50 μg of cellular proteins were mixed with 5x loading buffer and boiled for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... filtered through a Millex-HV 0.45 µm low protein binding PVDF membrane (Millipore), and concentrated 100-fold using Lenti-X Concentrator (Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were then concentrated using Amicon Ultra centrifugal filters (30k MWCO, Millipore). The protein concentration of eluates were estimated with a Bradford assay (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: ... Fractions containing protein were concentrated in 50-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2023Quote: ... fractions containing protein were concentrated in 10-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Biophysics 2023Quote: ... the protein was digested online by a pepsin/FPXIII (Sigma-Aldrich P6887/P2143) mixed protease column maintained at 20 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was transferred to an Immobilon®-FL PVDF Membrane (Millipore; IPFL00010) using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated with ultrafiltration centrifugal devices of 50-kDa cutoff (Amicon, Millipore). Monodispersed fractions of proteins were collected from size-exclusion columns (Superdex 200 10/300 GL ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 using a protein concentrator with a 10-kDa cutoff (Millipore, UFC801024 before loading onto a HiTrap Q HP column connected to an ÄKTA FPLC ...
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bioRxiv - Bioengineering 2022Quote: ... Tissue punches were suspended in protein extraction RIPA lysis buffer (Sigma-Aldrich Corp) containing protease and phosphatase inhibitors cocktail (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using a 50 kDa cut-off Amicon device (Millipore). The concentrated protein was centrifuged at 14,000 xg for 15 mins before injecting onto a Superdex 200 (10/300 ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was concentrated with centrifugal concentrators (UFC901096, Merck Millipore, Cork, Ireland), and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96 ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were subsequently incubated for 2 h with Protein-G–Sepharose (Sigma-Aldrich). After 3 washes with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was transferred by electrophoresis to an Immobilon-P PVDF membrane (Millipore Corp.).
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bioRxiv - Cell Biology 2022Quote: ... the isolated proteins were eluted in 25 µL 2x Laemmli buffer (Sigma Aldrich) by boiling for 5 min at 95 °C and the supernatant prepared for mass spectrometry measurement.