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Citations for Millipore Sigma :
5551 - 5600 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Bioengineering 2023Quote: ... ethylenediaminetetraacetic acid (EDTA), iodoacetamide (IAM), urea, recombinant porcine trypsin, K2IrCl6, and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO). L-histidine monohydrochloride monohydrate and L-histidine were purchased from Avantor (Allentown ...
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bioRxiv - Microbiology 2024Quote: ... for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for QD8 production ...
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bioRxiv - Neuroscience 2024Quote: ... we used our LPS-sensitized model of HI described above and delivered IP 1.5 IU/g of hCG diluted in normal saline (Sigma-Aldrich, St. Louis, MO, Catalog no. CG-10) or normal saline alone as vehicle control two hours prior to LPS administration (Figure 2A) ...
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bioRxiv - Plant Biology 2021Quote: ... blocked with 3% milk for 1 hour at room temperature and probed with anti-FLAG M2 monoclonal antibody (mouse, Sigma F1804, 1:2000 in 3% milk/PBST), or blocked with 5% BSA for 1 hour at room temperature and probed with anti-HA antibody (rabbit ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... The reaction products were analyzed on 8% PAGE-SDS gels with silver staining or Western blotting for PAR using a recombinant anti-poly-ADP-ribose binding reagent (Millipore, Cat# MABE1031; RRID: AB_2665467).
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were used for purification of recombinant proteins by 2 h incubation with GST-agarose beads at 4 °C according to manufacturer’s protocol (Sigma Aldrich, St. Louis, USA, G4510). SDS-PAGE electrophoresis was used to check level of expression and purity of purification.
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bioRxiv - Cell Biology 2024Quote: One rabbit was immunized with 500 μg of recombinant arpin by subcutaneous injection of an emulsified 1:1 mixture of protein and TiterMax Gold (Sigma Aldrich; St. Louis, MO). Three more immunizations were performed 14 ...
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bioRxiv - Biophysics 2021Quote: ... and blocked with blocking buffer (3% bovine serum albumin (BSA, Sigma-Aldrich) and 0.05% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: NmeCas9 was expressed in Rosetta 2(DE3) cells (Millipore Sigma, 71400-3) and grown in LB supplemented with 100 µg/mL Carbenicillin and 34µg/mL Chloramphenicol ...
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bioRxiv - Cell Biology 2020Quote: ... a thymidine block was performed by adding 3 mM thymidine (Sigma-Aldrich) for 16 h ...
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bioRxiv - Systems Biology 2020Quote: ... and 0.25 U/µl benzonase (Millipore Benzonase Nuclease HC, Ref#71206-3). Concentrated stocks of the lysis buffer were always freshly prepared and added volumes were adjusted to the remaining PBS supernatant in the cell pellets ...
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bioRxiv - Cell Biology 2019Quote: ... and 500 μM auxin (3-indole-acetic acid, IAA, Sigma, I-5148) for optimal depletion of SAF-A-AID-mCherry.
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bioRxiv - Immunology 2021Quote: ... it was added the substrate 3-Amino-9-ethylcarbazole (AEC) (Sigma-Aldrich), for 20 minutes ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... and developed with chromogen substrate: 0.03% 3-amino-9-ethylcarbazole (Sigma-Aldrich) and 0.015% H2O2 in 1M sodium acetate ...
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bioRxiv - Cell Biology 2019Quote: ... rerio embryos with or without chorion were mounted in 3 % methylcellulose (Sigma) in a small petri dish ...
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bioRxiv - Cell Biology 2020Quote: ... organoids were embedded in 3% low-gelling temperature agarose (Sigma-Aldrich, A9414) in HBSS without Ca2+ and Mg2+ (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 10µM of CK-666 (Arp2/3 Complex Inhibitor I, 10µM, Sigma-Aldrich), ML141 (Cdc42 inhibitor ...
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bioRxiv - Immunology 2021Quote: ... incubated for 1 hr in TBS-T + 3% milk + α-FLAG (Sigma) or a-MYC (1:4,000 dilution ...
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bioRxiv - Genetics 2021Quote: ... they were moved onto a thin layer of 3% methylcellulose (Sigma-Aldrich) and oriented for a lateral or top view ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were lysed in 3 ml BugBuster HT (Millipore, #70922-4) for 30 min at room temperature on a rotator ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 10 mg/mL SB3-14 (3-(N,N-Dimethylmyristylammonio)propanesulfonate (Sigma T7763)) at 37°C for 1hr and 95°C for 10min ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 dpf larvae were incubated with 10 mM Mtz (Sigma-Aldrich, M3761) dissolved in E3 medium containing 1% DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... Pairs of coverslips were made to sandwich 3-glycidyloxypropyl trimethoxysilane (440167, Sigma) on the marked sides ...
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bioRxiv - Molecular Biology 2020Quote: ... binding was visualized using 3-amino-9-ethylcarbazole (AEC) (Sigma-Aldrich, Germany) and slides were counterstained using hematoxylin.
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bioRxiv - Bioengineering 2022Quote: ... a 0.1-M solution of 3-mercaptotrimethoxysilane (Gelest) in acetonitrile (99.8%; Sigma) is flushed through the electrode channels and blown dry with pressurized air ...
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bioRxiv - Bioengineering 2022Quote: ... a 0.1 M solution of 3-mercaptotrimethoxysilane (Gelest) in acetonitrile (99.8%; Sigma) was flushed through the electrode channels and blown out with pressurized air ...
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bioRxiv - Cell Biology 2022Quote: ... Sin1 (3-(4-5 Morpholinyl) sydnone imine hydrochloride (Sigma-Aldrich, Cat-M5793). Media products MEM and F12 (ratio 1:1) ...
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bioRxiv - Cell Biology 2022Quote: INS-1 cells and INS-1 823/3 cells (Sigma-Aldrich, scc208) were cultured in RPMI-1640 medium ...
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bioRxiv - Cell Biology 2022Quote: ... before incubation with blocking buffer (3% nuclease-free bovine serum albumin (Sigma) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μM CK666 (to inhibit the Arp2/3 complex; Calbiochem/Sigma, 182515), 100 μM CK689 (an inactive control for CK666 ...
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bioRxiv - Developmental Biology 2022Quote: ... and embedding tissues in Paraplast (Sigma-Aldrich, Cat. No. 145686-99-3). Histological sections were made at five microns and stained with Ehrlich-Eosin Hematoxylin (Sigma-Aldrich ...
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bioRxiv - Physiology 2019Quote: ... All experiments used FITC-dextran (3-5kDa, Sigma Aldrich, St. Louis, USA) a commonly used probe for determining paracellular permeability in both invertebrate and vertebrate models such as fruit flies (D ...
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bioRxiv - Bioengineering 2019Quote: hMSC condensations (N = 3/group) were homogenized in TRI Reagent (Sigma-Aldrich) for subsequent total RNA extraction and cDNA synthesis (iScript™ kit ...
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bioRxiv - Developmental Biology 2019Quote: ... washed 3 times in PBS and incubated in DAPI (1:1000, Sigma) for 20 minutes at RT ...
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bioRxiv - Immunology 2019Quote: ... After blocking with PBS containing 3 % of bovine serum albumin (BSA) (Sigma) for 1 hr at room temperature (RT) ...
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Presynaptic mitochondria volume and abundance increase during development of a high-fidelity synapsebioRxiv - Neuroscience 2019Quote: ... Tissue was infiltrated using 3:1 PO to Durcupan resin (Sigma Aldrich) for 2 hours ...
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bioRxiv - Bioengineering 2019Quote: The organic phase was prepared by mixing Solvent Green 3 (Sigma–Aldrich) and chloroform (Fischer Chemical ...
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bioRxiv - Immunology 2019Quote: ... the MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) powder is re-suspended into PBS ...
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bioRxiv - Immunology 2020Quote: ... and 3’-amino-9-ethyl carbazole (AEC Tablets, Sigma, St. Louis, MO) were further used to develop the plate ...
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bioRxiv - Developmental Biology 2019Quote: ... HA (1:2000 in 3% skim milk/TBST, clone HA-7, Sigma), myc (1:400 in 5% BSA/TBST ...
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bioRxiv - Neuroscience 2020Quote: ... Other reagents used the ASMase inhibitor desipramine (10 µM, 3 h, Sigma), the LXRα agonist T0901317 (1 µM for 16 h ...
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bioRxiv - Microbiology 2019Quote: ... the DMEM containing 3% FBS and 4% sodium carboxymethyl cellulose (CMC) (Sigma) were added to the cells and cultured for 5 days ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with 20nM BubR1 siRNA (5′-AGAUCCUGGCUAACUGUUC-3’) (Sigma-Aldrich) or 20nM Knl1 dsiRNA (5’-ATGCATGTATCTCTTAAGGA AGATGAA-3’ ...
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bioRxiv - Microbiology 2020Quote: ... cells were gently washed 3 times with DPBS (Sigma, St Louis, MO). Finally ...
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bioRxiv - Biophysics 2019Quote: ... were blocked in 3% (w/v) fatty acid-free BSA (Sigma-Aldrich) in TBST (50 mM Tris-HCl ...