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Citations for Millipore Sigma :
5551 - 5600 of 10000+ citations for Mouse Anti Hepatitis C Virus NS5a Antibody 1877 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies were rabbit anti-phosphorylated ERK (1/500; Tasaki et al., 2011a) or mouse anti-α-tubulin monoclonal antibody DM 1A (1/5,000; Sigma-Aldrich, T9026). An appropriate antibody conjugated with horseradish peroxidase (1/5,000 ...
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bioRxiv - Immunology 2020Quote: ... Total IgG response was evaluated by enzyme-linked immunosorbent assays (ELISAs) using 1:5000 dilution of HRP conjugated anti mouse secondary antibody (A4416, Sigma-Aldrich, USA).
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bioRxiv - Neuroscience 2022Quote: ... whereas transfected Flag-Glt-1 cells were incubated overnight with a mouse anti-Flag® primary antibody (1:200-Sigma Aldrich; #F1804) that recognized the DKK sequence ...
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bioRxiv - Neuroscience 2021Quote: ... Free-floating sections were rinsed in phosphate-buffered saline (PBS) and then incubated overnight at 4ºC under constant agitation with the antibody (mouse anti-NeuN (Millipore, 1:500, MAB377), goat anti-Versican (R&D ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies and their concentrations were as follows: 1:1000 mouse-anti-α-SMA (Millipore Sigma; Burlington, MA; clone ASM-1/1A4), 1:300 rabbit-anti-Daam1 (Proteintech ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred from the gel to a PVDF membrane using a semidry transfer and probed with a mouse monoclonal anti-puromycin primary antibody (Millipore, Cat# MABE343), followed by incubation with an anti-mouse secondary antibody (Jackson Immuno Research ...
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bioRxiv - Biophysics 2020Quote: ... was used as secondary antibody for the microtubule and single molecule surfaces staining and goat anti-mouse IgG (Sigma-Aldrich, SAB3701063-1) was used for β-tubulin immunostaining in FLIM experiments ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were incubated for one hour at room temperature with a monoclonal anti-γ-tubulin antibody produced in mouse (Sigma-Aldrich, T5326), diluted 1/1000 in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS at 37°C for 30 minutes and subsequently incubated for two hours with a 1:200 concentration anti-glial fibrillary acidic protein (GFAP) antibody produced in mouse (Sigma, 1:200) and 1:500 concentration anti-β-Tubulin III antibody produced in rabbit (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... the following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Immunology 2021Quote: ... subsequently lysed and postnuclear supernatants were incubated o/n with monoclonal mouse anti-HA-antibody coupled to agarose beads (clone HA-7, Sigma-Aldich, USA) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Tubulin or actin were used as loading controls and the detection was done by using anti-tubulin antibody (Sigma T5168, mouse monoclonal) or anti-actin antibody (ab14128 ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were blocked in 10% horse serum made in 0.1M Phosphate Buffer with 0.3% Triton-x (PBTx) for two hours followed by overnight incubation with the following primary antibodies: mouse anti-Parvalbumin (1:1000, Sigma Aldrich, P3088), goat anti-Somatostatin (1:350 ...
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bioRxiv - Neuroscience 2021Quote: The following primary antibodies were used in the study: mouse anti-tubulin beta III isoform (Tuj1),clone TU20 (MAB1637, Millipore, 1:1000); rabbit anti- β3-Tubulin ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was subsequently blocked using 1% casein in 1x PBS and incubated with primary antibody solutions with following dilutions: mouse anti-HA (Sigma, 1:500), rabbit anti-HSP101 (r950 ...
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bioRxiv - Cancer Biology 2020Quote: Two biological replicates for each knock-down/rescue sample were analyzed by CUT&RUN using the anti-FLAG M2 mouse antibody (Sigma F1804-200UG) as described and sequenced with the Illumina HiSeq4000 (32) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... free floating sections were incubated with mouse anti-FLAG clone M2 antibodies (1:2000 in the donkey serum blocking solution, Sigma-Aldrich F1804), overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibodies and their dilution rates used for immunostaining in this study are as follows: mouse monoclonal anti-GFAP (MAB360; Millipore, Billerica, MA) at 1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... The input and elution samples were analyzed by Western blot by probing with mouse anti-FLAG M2 antibody (Sigma Aldrich; 1:2000), rabbit anti-His tag antibody (Cell signaling ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The ratio of protein level or phosphorylation levele against control β-actin protein level (detected with a mouse monoclonal Anti-β-Actin-Peroxidase antibody (A3854 Sigma)) was normalized to untreated wt sample mean.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with Odyssey blocking buffer (Li-COR) followed by incubation with antibodies specific for GFP (mouse monoclonal anti-GFP [7.1 and 13.1] (Sigma-Aldrich Cat# 11814460001, RRID:AB_390913)) and Tub1 (rat monoclonal anti-tubulin alpha ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) for 4 hours at 4°C with rotation ...
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bioRxiv - Bioengineering 2019Quote: ... MA) and the secondary antibody used was HRP-conjugated goat polyclonal anti-mouse IgA (1:500; A4789; Sigma Aldrich; St. Louis, MO). HRP was detected with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were used at 1:1000 and included: mouse monoclonal anti-β1/β2-adaptins (clone 100/1; Sigma, St. Louis, MO); mouse monoclonal anti-clathrin heavy chain (clone 23 ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... The primary antibody used for Western blot was a horseradish peroxidase conjugated goat anti-mouse IgG (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Systems Biology 2020Quote: ... Resolved proteins were transferred to nitrocellulose membranes and detected by standard Western blotting using monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and HRP labelled anti-mouse IgG secondary antibody (Cell Signalling technology ...
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bioRxiv - Genomics 2021Quote: ... Cells were immunostained with rabbit antibody against Histone H3 (phospho S10) (1: 5000) to identify mitotic cells and mouse anti-Tubulin (Sigma; 1:500) to assay cell viability and mitotic stage as previously described55 ...
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bioRxiv - Genomics 2021Quote: ... Eluted proteins were then analyzed by western blotting with anti-U1-70k mouse monoclonal antibody (9C4.1) (05-1588, Merk Millipore, Burlington, MA, USA) at a dilution of 1:500 for the detection of U1-70k ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (diluted in 5% BSA in 1X PBS-T) were as follows: Mouse anti-β-Actin (1:10,000, Sigma-Aldrich, A5441), Rat anti-α Tubulin (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... then washed twice in PBS and incubated 1 hour with the tertiary antibody goat anti-mouse whole molecule conjugated to FITC (Sigma, 1:400). The intrabody anti-GFP nanobody 3 x HA-tagged (INbGFP::3HA ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) or 5ug Normal Mouse IgG Polyclonal Antibody (MERCK ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were analysed using 4-12% Bis-Tris polyacrylamide gels in MOPS buffer followed by Coomassie staining or western blot using the following antibodies: Mouse anti-FLAG M2 (Sigma, 1:10,000). RNA was isolated from TriReagent according to the manufacturer’s protocol and then reverse transcribed using the high capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore, MAB1574, 1:5,000).
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bioRxiv - Neuroscience 2022Quote: ... Larvae were washed three times in 0.1% PBST and incubated with secondary antibodies: anti-mouse Alexa Flour 647, 1:100 (Invitrogen, #28181. Stained larvae were mounted using DAPI Fluoroshield (Sigma-Aldrich, #F6182). Images were collected on a Nikon A1 eclipse Ti confocal microscope.
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated for 1 hour at room temperature with a goat anti-mouse alkaline phosphatase-conjugated secondary antibody (dilution 1: 5000; Sigma-Aldrich, USA) and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Neuroscience 2022Quote: ... immunohistochemical examination of all surgical brain specimens was performed using the following panel of antibodies: mouse monoclonal anti-NeuN (clone A-60, Millipore Cat#MAB377), mouse monoclonal anti-GFAP (clone 6F2 ...
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bioRxiv - Neuroscience 2022Quote: ... mid-sagittal cerebellar vibratome sections were blocked and permeabilized with 10% normal goat serum in PBS containing 0.2% Triton X-100 and immunostained with a mouse anti-Calbindin D28k antibody (Sigma-Aldrich, 1:1000) followed by incubation with Alexa Fluor 555-labeled goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The membranes that were loaded with fetal membrane and uterine tissue lysates were re-probed for 1 h at room temperature with a mouse anti-β-actin (ACTB) monoclonal antibody (Cat# A5441, Sigma-Aldrich). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells on coverslips were blocked with blocking solution (2% BSA/PBST) for 30 min and incubated with the primary antibodies anti-Flag (mouse, Sigma, M2, F1804), anti-PABPN1 (rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... The upper part of the membrane was incubated with a primary monoclonal mouse-anti-HA antibody (1:1000; H3663; Sigma-Aldrich, USA) to detect HA-tagged TRPM2 channel domain and the lower part of the membrane was incubated with rabbit-anti-FLAG (DYKDDDDK ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4 °C with BB containing a primary antibody cocktail (1:150 mouse anti-ionized calcium-binding adapter molecule 1 (Iba1; EMD-Millipore cat# MABN92+), 1:300 rabbit anti-transmembrane protein 119 (TMEM119 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins of the Co-IP were analyzed by Western Blot with the Monoclonal ANTI-FALG® M2 antibody produced in mouse (Sigma Aldrich), proteins of inputs were analyzed by Western Blot with the Anti-GFP from mouse (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 16-h incubation in PBS with 0.1% Triton™ X-100 with the following antibodies: mouse monoclonal anti-NeuN (1:500; EMD Millipore, MAB377), rabbit polyclonal anti-KCC2 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... to block non-specific staining and incubated with a mouse anti-acetylated tubulin antibody (Clone 6-11b-1, 1:100; Sigma Cat# T6793) for 4 h at room temperature ...