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Citations for Millipore Sigma :
5551 - 5600 of 10000+ citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The fragment was cloned between the SalI and NotI restriction sites of the pET23a(+) plasmid (Novagen) resulting in a pEt23_CAP-D2_Ct construct which contains the cassette T7 promoter∷T7 tag-CAP-D2Ct-His tag ...
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bioRxiv - Cell Biology 2019Quote: ... Hela and HAP1 cells were transfected with 1 µg of corresponding plasmid using GeneJuice® (Novagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: HK2 cells were transfected with different plasmids using X-tremeGENE HP DNA transfection reagent (Sigma-Aldrich) according to the manufacturer’s instructions and incubated for 18-24 h before fixation or live-cell imaging ...
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bioRxiv - Synthetic Biology 2021Quote: ... the truncated AtCCD7 gene was amplified by PCR and cloned into the pCDFDuet-1 plasmid (Novagen) using NcoI and NotI to yield the plasmid pCDFDuet-trAtCCD7 ...
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bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
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bioRxiv - Pathology 2021Quote: ... Purification of plasmids was achieved using the Endotoxin-free Maxi prep kit (Sigma-Aldrich, St.Louis, MO), after which the constructs were injected into the mice.
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bioRxiv - Cell Biology 2022Quote: ... 5’-AGTTTCTCTGTCCGATTTAAA-3’] are in the plasmid backbone pLKO.1-CMV-tGFP and were from Sigma Millipore (St Louis ...
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bioRxiv - Developmental Biology 2022Quote: ... GJA1 siRNA- or plasmid DNA-transfected RPE1 cells were cultured on fibronectin (Sigma Aldrich, MO, USA)-coated cover glasses ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting PCR products were ligated into the same sites in plasmid pET-22b(+) (Novagen). An identical strategy was used to generate plasmids pET-YpTatB22-220 and pET-YpTatB22-91 for expressing and purifying full-length or truncated versions of TatB from Y ...
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bioRxiv - Immunology 2020Quote: ... DNA plasmids were transfected into Lenti-X™ 293T cells using GeneJuice® (Novagen, Waltford, UK) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Plasmids were extracted using the Qiagen Miniprep Kit using 425-600 μm glass beads (Sigma Aldrich) for mechanical lysing of yeast cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was isolated from the transformant and transformed into Rosetta (DE3) cells (Merck Millipore, USA). For purification ...
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bioRxiv - Cell Biology 2022Quote: ... HK2 cells were transfected with different plasmids using X- tremeGENE HP DNA transfection reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... cells transfected with the KRASWT plasmids were always supplemented with 15ng/ml of doxycycline (Sigma-Aldrich). Cells were incubated for 24 h at 37°C and harvested ...
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bioRxiv - Molecular Biology 2022Quote: ... and HEK293T cells were transfected with pcDNA3.1/PA-hZnT7ΔHis-loop plasmids using PEI reagent (Sigma-Aldrich) at a ratio of 50 μg DNA to 1.5 ml PEI reagent (1 mg ml-1) ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloning of it into NdeI-XhoI site of expression plasmid pET-22b(+) (Novagen, WI, USA).
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bioRxiv - Neuroscience 2024Quote: ... a mixture of PEI and Plasmid DNA diluted in DPBS (Dulbecco Phosphate Buffered Saline) (Sigma; D8537) was added to the cell culture media to a final concentration of ...
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bioRxiv - Cell Biology 2023Quote: ... p15A-CmR backbone plasmids (see Table S2) were maintained using 30 µg/mL chloramphenicol (Sigma-Aldrich) in plates ...
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bioRxiv - Neuroscience 2024Quote: A mix of endotoxin-free plasmid preparation (2-5 mg/mL) and 0.5% Fast Green (Sigma) mixture was injected using a Picospritzer III (Parker ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng plasmid DNA was combined with 1.5 µL polyethyleneimine (PEI, 1 mg/mL, Sigma, #p3143) in 50 µL Optimem (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Plasmid constructs pLKO.1/shMfrn1 (TRCN0000044010) and pLKO.1/shFTH1 (TRCN0000029432) were purchased from Sigma-Aldrich, and V5-tagged FSP1 (V5-FSP1 ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized plasmid was subsequently ligated to oligo pairs to create long ssDNA overhangs (Sigma-Aldrich) on either end ...
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bioRxiv - Neuroscience 2023Quote: ... and mock plasmids were transfected into HEK293 suspension cells derived from tsA201 (Millipore Sigma, 96121229-1VL) using Polyethylenimine (Polysciences ...
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bioRxiv - Developmental Biology 2023Quote: ... predicted mature peptide regions of ZmRALF1/2/3/5 genes were cloned into plasmid pET32b (Novagen) with gene specific primers as indicated (Supplemental Table S1) ...
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bioRxiv - Microbiology 2023Quote: ... and FLAG-tagged UL31 plasmid constructs using of X-tremeGENE HP DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and FLAG-tagged UL31 plasmid constructs using of X-tremeGENE HP DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The plasmids for prokaryotic expression are pGEX-6P-1 (NovaPro #V010912) and pET-28a (Novagen # 69864). Amino acid sequences of inserted genes were verified by bi-directional sequencing analyses.
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were transformed into Escherichia coli BL21(DE3) CodonPlus cells and grown in Terrific Broth (Sigma) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2, Sigma, Cat #: F1804, 1: 500 dilution), or anti-SARS-CoV-2 human sera (1:100 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit polyclonal anti-mouse IBA1 (Wako ref. 019-19741, RRID AB_839504, 1:1500) and rabbit polyclonal anti-mouse NF200 (Sigma-Aldrich ref. N4142, RRID AB_477272, 1:500). The following day slices were washed with PBS (2×10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies for immunofluorescence include mouse anti-Yap1 (Sigma-Aldrich, WH0010413M1)(1:33 ...
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bioRxiv - Cancer Biology 2021Quote: ... and anti-actin mouse monoclonal antibody (1:2000, AC-40, Sigma).
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bioRxiv - Microbiology 2021Quote: ... and Mouse IgE Single Plex Magnetic Bead Milliplex MAP kit (Millipore). Samples were diluted 1:12500 (IgA ...
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bioRxiv - Molecular Biology 2020Quote: ... R Mouse monoclonal to Anti- β-actin (Sigma #A5316, 1:3000), Rabbit polyclonal to GAPDH (abcam #ab9485 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-PSTAIR (Sigma Aldrich Chemie GmbH, cat# P7962, 1:50’000), mouse anti-FLAG M2 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... and monoclonal mouse anti-β-actin (Sigma-Aldrich, #A5316, 1:2000), anti-Cdc42 (BD Transduction Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse monoclonal antibody against actin (clone C4) was from Millipore (#MAB1501). Rabbit polyclonal antibody against NFATc3 was from Proteintech (18222-1-AP) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Ac-Tuba1a (Sigma; Cat# T7451, used at 1:500), RRID rabbit anti-p-SMAD1/5/8 (Cat#AB3848-I ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-KRT14 (Cat#CBL197; Millipore Sigma; used at 1:200), mouse anti-MMP7 (Cat#MAB3315 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-MMP7 (Cat#MAB3315; Millipore Sigma; used at 1:200), mouse IgM anti-VIMENTIN (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-α-tubulin antibody (Sigma-Aldrich, #T5168, WB 1:10,000), mouse anti-ß-actin antibody (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-ß-actin antibody (Sigma-Aldrich, #A5441, WB, 1:10,000), Rabbit anti-p34-Arc/ARPC2 (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-γH2AX IgG (1:200, JBW301, Merk Millipore, Massachusetts, USA). Negative controls were incubated without the primary antibody under the same conditions ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-myc 488 conjugated was purchased from Millipore (#16-308). Superfrost plus tissue slides (Menzel-Gläser ...
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bioRxiv - Cell Biology 2020Quote: ... anti-talin (T3287, mouse monoclonal, clone 8D4, lot 035M4805V; Sigma-Aldrich), anti-GEF-H1 (ab155785 ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-β-actin (1:10,000, mouse mAb, Sigma-Aldrich, A5316). The following HRP-conjugated secondary antibodies were used for western blotting ...
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bioRxiv - Immunology 2021Quote: ... The primary antibody (mouse anti-GFAP from Sigma at 1:1000) diluted in 0.5M TBS-Triton-X-100 (0.5% ...
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bioRxiv - Developmental Biology 2021Quote: ... and mouse anti-γ-tubulin (T5326, 1:5000) from Sigma-Aldrich.
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-GFAP (Cy3 conjugated, cat. # MAB3402C3, Sigma-Aldrich, 1:500), rabbit anti-Cleaved CASPASE3 (cat ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies were used: anti-Th (mouse; 1:300; Millipore), anti-GFP (chicken ...