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Citations for Millipore Sigma :
5501 - 5550 of 10000+ citations for 6 CHLORO 4' METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Bioengineering 2021Quote: ... at −20°C for 6 minutes followed by a 1-hour incubation in blocking buffer containing 10 wt% BSA (Sigma) in PBS as previously described7 ...
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bioRxiv - Developmental Biology 2020Quote: ... The pregnant mice were serially scanned at E6.5 either after intra-peritoneal administration of 1µg/g BW of hyaluronan synthesis inhibitor 6-diazo-5-oxo-1-norleucine (DON, Sigma-Aldrich) at E3.5-E5.5 ...
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bioRxiv - Cell Biology 2020Quote: ... fixed accessory glands were permeabilised for 6 x 10 min in PBST (1 X PBS, 0.3% Triton X-100 [Sigma-Aldrich]), blocked for 30 min in PBSTG (PBST ...
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bioRxiv - Cell Biology 2019Quote: ... The resin was resuspended in 6 ml ice-cold G-buffer containing 5 µg/ml TLCK-treated chymotrypsin (Sigma, #C3142) and incubated overnight at 4°C ...
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bioRxiv - Physiology 2020Quote: ... rubens (< 6 cm diameter) were fixed by immersion in seawater Bouin’s fluid [75% saturated picric acid (Sigma-Aldrich, Gillingham, UK) in seawater ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transfected with HSIV-vif plasmids using Fugene 6 or X-tremeGENE 9 DNA transfection reagent (Roche/Sigma). At 48 hour post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse monoclonal anti-Na+/K+ ATPase (1:500; SantaCruz) and anti-acetylated tubulin (1:2,000; clone 6-11B-1, Sigma Aldrich). Goat anti-mouse or rabbit IgG conjugated with HRP were used for secondary antibodies (1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... two shRNA targeting exon 5 and exon 6 were synthesized along with a control shRNA targeting the luciferase gene as a 97-nt ultramer (Sigma) containing 22-mer reverted repeats ...
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bioRxiv - Cell Biology 2020Quote: Total protein from confluent passage 1 Sirt5−/− and WT chondrocytes cultured in 6 cm dishes were collected in RIPA buffer containing 20 mM nicotinamide (Sigma) and protease inhibitors cocktail (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... electroporated parasites were mixed immediately with 100 μl of reticulocyte-rich blood from a naïve mouse treated with phenylhydrazine (6 mg/ml) (Sigma-Aldrich), incubated at 37°C for 20 min and then injected intraperitoneally into another mouse ...
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bioRxiv - Cell Biology 2021Quote: ... or control non-target shRNAs were induced to differentiate for 6 days and RNA was extracted with Tri-Reagent (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 3 µg of RNA were separated on MEN-buffered 1.5% agarose gels that provided denaturing conditions by 6% formaldehyde using an RNA sample loading buffer containing a final concentration of 62.5% (v/v) deionized formamide (Sigma-Aldrich). Afterwards ...
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bioRxiv - Microbiology 2021Quote: ... The enzyme reaction was started by the addition of 0.16 ml of 6 mM p- nitrophenyl β-D-glucuronide (Sigma) to the broken cells and stopped by the addition of 0.4 ml of 1.0 M NaCO3 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then treated with 6-thio-dG (3-5 μM) in the presence of 500 ng/ml doxycycline (Sigma), washed three times with warm PBS and allowed to recover in regular growth medium containing 500 ng/ml doxycycline ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then analyzed on a Guava EasyCyte 6/2L bench cytometer equipped with 488 nm and 532 nm lasers (Millipore).
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bioRxiv - Microbiology 2020Quote: ... Cells were then fixed with 1% formaldehyde solution and analyzed using a Guava EasyCyte 6/2L bench cytometer equipped with 488 nm and 532 nm lasers (Millipore).
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bioRxiv - Immunology 2021Quote: ... were characterized and fractionated by size exclusion chromatography using a Superose 6 10/300 GL on an AKTA liquid chromatography system and concentrated on AmiconUltra 100k spin columns (Millipore).
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bioRxiv - Microbiology 2021Quote: ... before cells were fixed with 1% formalin for 30 minutes and stained using Giemsa stain (SIGMA-ALDRICH®, 6% diluted) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: Total splenocytes were isolated from C57BL/6 and mFICD-/- mouse spleens and then treated for 3 days with 20 μg/ml LPS (Sigma), 100 µg/ml heparan sulfate or 2.5 μM thapsigargin (Enzo Life Sciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... Trx-His(6)-Δlinker-hSLAP2 or Trx-His(6)-mSLAP were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Immunology 2020Quote: ... grown at the log-phase in 6-well plates in the presence of DMEM supplemented with 1% FBS and 0.5% (w/v) methylcellulose (Sigma-Aldrich). After 2 d of incubation at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Eluate from amylose resin was concentrated and purified as a range of small oligomers on a Superose 6 size exclusion column (Cytiva) in lysis buffer, concentrated again (500 μL, A280 = 6) using a 50 kDa spin column (Millipore), and cleaved overnight with MBP-3C protease (50 μL ...
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bioRxiv - Cell Biology 2020Quote: ... were seeded at 200,000 cells/well of 6 well plates and transduced with lentiviral particles at final MOI of 0.5 with 8 µg/ml polybrene (Sigma-Aldrich). Transduced cells were selected in 10 µg/ml puromycin (Gibco ...
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bioRxiv - Immunology 2021Quote: ... or 0.5 μM/ml 5-amino-6-D-ribitylaminouracil (5-A-RU) (courtesy of Jeffrey Aubé, UNC) and 50 μM/ml Methylglyoxal (Sigma). Brefeldin A Solution (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... then resuspended in complete DMEM for analysis by flow cytometry on a Guava EasyCyte 6/2L bench cytometer equipped with 488nm and 532nm lasers (Millipore). Control wells were prepared without sporozoites to measure the basal level of dextran uptake ...
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The intestinal circadian clock drives microbial rhythmicity to maintain gastrointestinal homeostasisbioRxiv - Physiology 2022Quote: Complete GI transit time (GITT) was measured by administering natural carmine red (6%, SigmaAldrich) dissolved in 0.5% methylcellulose (Sigma-Aldrich) by gavage (100ul ...
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bioRxiv - Cancer Biology 2022Quote: ... see Figure S1A and 22) added to the cells with 400 µg/ml of poloxamer-407 (Sigma, 9003-11-6) and incubated in a 37°C CO2 incubator for another 24 hours (Figure 1A) ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5×105 B16-F10 cells were plated into a 6-well dish and stimulated with growth media supplemented with 10 or 20 μM forskolin (Sigma) and 100 μM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626), 0.4 mg/L biotin and 0.5% methanol ...
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bioRxiv - Biochemistry 2022Quote: ... with 3% BSA and incubated with the 3% BSA-blocked blotted membrane at 4 °C overnight in the absence or presence (as a negative control) of 10 mM free mannose-6-phosphate (M6P) (Sigma). The membranes were then washed three times with TBST (1×15 min and 2x 5min washes ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Developmental Biology 2022Quote: ... A few hours later the cells were infected with titer-adjusted volumes of each viral supernatant supplemented with 6 μg/ml (final concentration) of protamine sulfate (Sigma). After two days ...
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bioRxiv - Cell Biology 2022Quote: NOD/SCID mice (6 weeks-old) were treated with an intraperitoneal (ip) injection of 650 mg APAP/kg (Sigma-Aldrich) to induce ALF ...
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bioRxiv - Developmental Biology 2022Quote: ... 2/3 mTeSR1 + 1/3 N2 medium + LSX + PSD Day 6,7: 1/3 mTeSR1 + 2/3 N2 medium + PSD At day 6 cells were detached by Accutase solution (Sigma-Aldrich) and incubated at 37°C for 20 minutes to obtain a single cell suspension ...
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bioRxiv - Cell Biology 2022Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 (mouse ...
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bioRxiv - Cell Biology 2022Quote: ... The following incubations ensued at room temperature: 3×10 min in 0.1 M maleic acid buffer (Sigma-Aldrich; pH 6), 2 h in the dark in 0.5% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... The explants were cultured for 6 days in DMEM with 10% fetal bovine serum and 10 μg/ml ciprofloxacin (17850, Sigma), and then fixed in 4% PFA for 15min at room temperature before being processed for immunolabeling.
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bioRxiv - Immunology 2022Quote: ... Lysates were assayed for phospho proteins using the Milliplex Cell Signaling kit (NFκB Magnetic Bead Signaling 6-plex, Millipore Sigma) for detection of NFκB pSer536 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 µL of the mix (corresponding to 8.105 cells) was then plated on 6 cm Petri dishes filled with 2 mL of 2% Phytagel (Sigma-Aldrich), following Dubravcic et al (Dubravcic et al. ...
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bioRxiv - Immunology 2022Quote: ... synthesized as previously described),6 a 5 mg/mL stock solution of PBI1 dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich) was prepared ...
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bioRxiv - Plant Biology 2022Quote: ... total glutathione in 0.1 ml of the supernatant was measured by spectrophotometry in a 1 ml mixture containing 6 mM DTNB (Sigma-Aldrich), 3 mM NADPH ...
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bioRxiv - Immunology 2022Quote: Dorsal and ventral side ears of either Mas-TRECK mice or wild type (C57BL/6) mice (WT) were injected intradermally (i.d.) with 10 ng of diphtheria toxin (DT) (Sigma-Aldrich) in 20 µl of PBS for one time ...
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bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... the embryos were arranged in glass Petri dishes (90 × 15 mm) and immersed in 6 mL stationary MS liquid culture medium (Murashige and Skoog, 1962) (Sigma) containing 0.1 g L-1 myo- inositol (Sigma) ...
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Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: ... Cochlear cultures were treated for 6 h with various reagents (all dissolved in culture medium): 0.1% (v/v) DMSO (276855; Sigma-Aldrich), 0.5 mM BAPTA-AM (A1076 ...
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bioRxiv - Neuroscience 2022Quote: ... The silk solution concentration was adjusted to 6 mg/mL and combined in a 10 cm dish with 400 to 500 µm sodium chloride (Sigma) particles at a ratio of 1:2 (v/w ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 mM NaCl and 6 mM β-mercaptoethanol) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and Protease inhibitor cocktail (Sigma-Aldrich) (20 mg/g of cell pellet) ...
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bioRxiv - Cell Biology 2022Quote: ... Parasitemia was adjusted to 0.2-0.5% and 2% hematocrit and the culture was distributed in triplicates in 6-wells plate with various treatments: 400pM of the mimic (HMI0513-5NMOL, Sigma) or 400pM of the inhibitor (cat# 4464084 ...