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Citations for Millipore Sigma :
5501 - 5550 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... 1.6μl of 20μM Preamp primer (5’-[Btn]TGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNN*N, SIGMA) and 32.8μl of Nuclease free water) ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 cells at ∼70% viability were spun down and incubated 3 min at 4°C in 25µl of a detergent buffer containing 0.2% Igepal CA-630 (Sigma, #I8896), 0.2% Tween 20 (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... The effect of GABABR antagonists on metamorphosis was tested using Phaclofen (10−3 M, 10−4 M, Sigma Aldrich) and the high affinity antagonist CGP-54626 (10−7 M ...
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bioRxiv - Systems Biology 2019Quote: ... Chk-2 inhibitor II BML-277 (used at 10 μM) from and Nutlin-3 (used at 0.75 - 4 μM, #N6287) from Sigma.
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bioRxiv - Immunology 2019Quote: ... Primers were designed using Primer3 software62 with sequences obtained from GenBank and synthesized by Sigma Aldrich (Table 3, 4). PCR reactions were performed on the LightCycler®480 Real Time PCR System (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... Col-0 plants grown on soil for 3-4 weeks were spray-treated with 0.5 mM SA (Sigma-Aldrich) and tissues harvested at indicated time-points for further analysis.
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally washed 3 times in PBS-Tween 20 0.1% and mounted in Mowiol 4-88 (81381, Sigma).
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bioRxiv - Neuroscience 2022Quote: ... GLASTCreERT2;Sun1-GFP mice at ∼3 weeks of age were intraperitoneally injected with 4-OHT (Sigma-Aldrich, #H6278-50mg) in corn oil (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... organoids in Matrigel on day 3 or 4 after passage were incubated with 100 μM rhodamine 123 (Sigma-Aldrich) in HBSS (Wako ...
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bioRxiv - Neuroscience 2022Quote: A model of hyperalgesic priming was used to induce chronic widespread pain in mice with consecutive injections of carrageenan into the right gastrocnemius muscle 4 days apart (i.m. 3% 30μl, Sigma Aldrich). Control mice only received one injection on D0 (30).
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bioRxiv - Immunology 2021Quote: ... and two pools of S2 peptides (pools 3 and 4) (final concentration of 2μg/mL) on IPVH-membrane plates (Millipore) coated with 5μg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Developmental Biology 2020Quote: ... were post-fixed with 4% para-formaldehyde and blocked with PBS solution containing 0.3% Triton-X 100 and 3% normal Donkey serum (Sigma). Antibody dilution buffer consisted of PBS containing 1% BSA and 0.3% Triton-X 100 ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Biochemistry 2024Quote: ... Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP, Sigma Aldrich) dissolved in 100 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
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bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged MSL proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Elution buffer ...
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bioRxiv - Microbiology 2023Quote: Infected S10-3 cells seeded onto coverslips were fixed in 4% PFA and permeabilized in 0.1% Trion-X100 (Sigma). For co-detection of RNA and capsid ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... and then blocked for 3 hr at room temperature or overnight at 4 °C in BSA (Sigma-Aldrich A2153) 3% in PBS-T ...
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bioRxiv - Developmental Biology 2019Quote: ... and homogenized in ATP assay Lysis buffer (6 M Guanidine-HCl, 100 mM Tris pH 8 and 4 mM EDTA, all from Sigma Aldrich, Cat# G3272 ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs (12×106 cells/mL) and MSCs (6×105 cells/mL) were suspended in EBM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... the acid insoluble fraction was incubated for 18 hours at 4°C with 500 μL of 6 M guanidine hydrochloride/100mM TRIS buffer (pH 7.4, Sigma-Aldrich, U.K.). The buffer was exchanged into 100 μL of 50 mM ammonium acetate (Scientific Laboratory Supplies ...
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bioRxiv - Cancer Biology 2022Quote: ... TILs and CAR-TILs were co-cultured with cancer cells for 4-6 hours in RPMI supplemented with human AB serum (Sigma-Aldrich) and CD107a antibody (clone H4A3 ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was immobilized on a nitrocellulose membrane and then probed with antibodies against (6-4)PPs (Cosmo Bio NM-DND-002) or ssDNA (Millipore MAB3034). Chemiluminescence was detected as for protein immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... Anti alpha tubulin-FITC mouse mAb (#F2168) and 4’,6-diamidine-20-phenylindole dihydrochloride (DAPI, #10236276001) were purchased from Sigma Aldrich, Germany ...
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bioRxiv - Genomics 2022Quote: ... a single thymidine block was used to first arrest cells in S phase: cells were plated at 4×10^6 cells per 15 cm plate in medium containing 2mM Thymidine (Sigma, T1895), and were grown for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: Mice 4-6 weeks old were given three doses 150 mg/kg or 200 mg/kg (i.p) tamoxifen (TAM) (Sigma-Aldrich T5648) dissolved in peanut oil (20 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... The spinal column was removed and 4-6 lumbar level DRG were dissected and placed onto coverslips coated with Poly-L-Lysine (Sigma, P8920). DRG were allowed to recover for 8 minutes (P4 ...
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bioRxiv - Cell Biology 2023Quote: ... Genetical labelling was induced in epidermis of 6-8 weeks old mice with a single topical administration of 75 μg of (Z)-4-Hydroxytamoxifen (Sigma-Aldrich) (15 mg/mL diluted in acetone).
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bioRxiv - Neuroscience 2023Quote: Mouse primary cortical neurons derived from Lrrk2 WT C57BL/6 and KO pups (P0) were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich) in PBS1X pH 7.4 for 20 minutes at room temperature and ...
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bioRxiv - Neuroscience 2024Quote: Mouse vibrissae were carefully isolated from the upper lip pad of 4-week-old C57BL/6 male mice and cultured in Williams E medium (Sigma-Aldrich), supplemented with 1% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs (4.5·106 cells/mL) and MSCs (6·105 cells/mL) were suspended in EGM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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bioRxiv - Bioengineering 2022Quote: ... crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Carl Roth GmbH + Co. KG) and N-hydroxysuccinimide (NHS) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was applied ...
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bioRxiv - Biophysics 2020Quote: Goat anti-mouse IgG was incubated using a 5 x excess of N-hydroxysuccinimidyl esters – Alexa647 (LifeTech, A20106) and purified after 4 h using spin-desalting columns (40K MWCO) in PBS (Sigma-Aldrich, D8537-500ML) to remove excess dyes ...
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bioRxiv - Biochemistry 2022Quote: ... and 78.1 µL of a 10 mg/mL (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-succinimidyl carbonate (BCN, 17 eq.) (Sigma Aldrich, Cat. No. 744867-10MG) and stirred at 4°C for 16 h ...
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bioRxiv - Microbiology 2022Quote: ... we filtered 500 ml of the collected water through a 0.22 μm Sterivex filter (Millipore Sigma, Burlington, MA; n=4 per time point) using peristaltic pressure ...
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bioRxiv - Physiology 2020Quote: Bone marrow from Winnie and control mice tibiae (n=4-8 mice/group) was collected by flushing with α-modified essential medium ((α-V1EV1, Sigma, Australia). Bone marrow-derived mesenchymal stromal cells (BM-MSCs ...
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bioRxiv - Neuroscience 2022Quote: The catalytic activity of NAGLU was determined by hydrolysis of the fluorogenic substrate 4-methylumbelliferyl-N-acetyl-α-glucosaminide (EMD Millipore, Burlington, MA) with a final concentration of 0.1 mM substrate in the incubation mixture as described previously50 ...
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bioRxiv - Pathology 2023Quote: ... washed in TBS (3 × 5 min) and blocked in 1% BSA (Sigma Aldrich) in TBST (0.1% Tween-20 in TBS) ...
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bioRxiv - Biochemistry 2023Quote: Two complementary single strand oligonucleotides (5’-GTAATCTGGGCCACCTGCCTGGGAGGA-3’ and 5’-TCCTCCCAGGCAGGTGGCCCAGATTAC-3’) corresponding E2 box44 were Two complementary single strand oligonucleotides (5’-purchased from Sigma-Aldrich. An equal molar ratio of single strand oligonucleotides was mixed and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... then fixed using 4% paraformaldehyde (Sigma). Cells were stored in PBS at 4ºC until processed for microscopy ...
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bioRxiv - Cell Biology 2020Quote: ... Doxycycline (4 g/L) (Sigma D9891) was continuously administered in drinking water containing sucrose (50 g/L ...
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bioRxiv - Immunology 2021Quote: 4% sterile Thioglycollate (Sigma, Cat# 70157) in ddH2O was applied to induce mice peritonitis ...
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bioRxiv - Cell Biology 2020Quote: 1ml of 4% thioglycollate (Sigma B2551) was injected directly into the peritoneal cavity of mice 6-8 weeks of age ...
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bioRxiv - Genomics 2021Quote: ... 4 mmol/L L-glutamine (Sigma), 100 μmol/L norepinephrine (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 mM L-glutamine (Sigma-Aldrich), 1X non-essential amino acids (Life Technologies ...