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Citations for Millipore Sigma :
5451 - 5500 of 5860 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... ∼2×107 parasites were collected by centrifugation (1,200 x g for 10 min) and pull-downs were performed using the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Sigma-Aldrich). Unenriched ‘input’ samples were generated by reserving back 10% of the starting lysate ...
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bioRxiv - Microbiology 2022Quote: ... while spotting them onto a polished stainless steel MALDI target in a 1:3 ratio (0.5 μL sample/1.5 μL matrix) with addition of 0.25 μL ProteoMass Normal Mass Calibration Mix (1:10 dilution, ProteoMass™ MALDI Calibration Kit, Sigma-Aldrich) for acquisition of high resolution spectra ...
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bioRxiv - Pathology 2022Quote: ... One ug total RNA was used for first strand cDNA synthesis of all samples Transcriptor High Fidelity cDNA kits (Sigma Aldrich) was used for first strand cDNA synthesis of all samples ...
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bioRxiv - Pathology 2022Quote: ... Leaf tissues were ground and homogenized in liquid nitrogen for subsequent total RNA isolation with the SpectrumTM Plant Total RNA Isolation Kit (Sigma, USA), followed by on-column DNase I digestion treatment (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... The 100 frozen leaves for each of these sample and the positive sample were extracted in pools of 25 following the Spectrum plant total RNA kit (Sigma-Aldrich). RNA extracts were tested by RT-PCR using the Titan One Tube RT-PCR kit (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... RNA from the original 100 frozen leaves of the positive sample were re-extracted in pools of 25 with the Spectrum plant total RNA kit (Sigma-Aldrich) and tested by RT-PCR using the Titan One Tube RT-PCR kit ...
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bioRxiv - Microbiology 2023Quote: ... The interactions between the two labeled proteins were detected using the Duolink in Situ Red Starter Kit Mouse/Rabbit (#DUO92101-1kit, Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and fed with StemFlex media (ThemoFisher Scientific) and undergo monthly mycoplasma testing to ensure cultures are mycoplasma-free with the LookOut Mycoplasma PCR detection kit (Millipore Sigma). For the differentiation of iPSC-derived microglia-like cells ...
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bioRxiv - Microbiology 2022Quote: DNA extraction was performed for each of the above isolated samples by following manufacturer’s instructions using the GenElute™ Bacterial Genomic DNA kit (Sigma-Aldrich). After gDNA extraction ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines tested negative for mycoplasma contamination using a PCR-based method using the VenorGeM Classic Mycoplasma Detection Kit (Sigma-Aldrich). STR-genotyping was performed to confirm the identity of HeLa-JVM ...
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bioRxiv - Molecular Biology 2022Quote: ... The tRNA-Pro probe sequence was amplified from this plasmid using the primers in Table S2 and in vitro transcribed by using Digoxigenin-11-UTP included in DIG Northern Starter Kit (Sigma, #12039672910). 25 µg total RNA each from WT and rad6Δ cells was resolved on 15% TBE-Urea polyacrylamide gel (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... which was then washed with up to 2.5 ml of digestion buffer (Tumour dissociation kit, Miltenyi, 130-096-730) plus Deoxyribonuclease I (300 μg/ml, Sigma, DN25-1G). Dissociation was performed using the protocol suggested by Miltenyi ...
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bioRxiv - Microbiology 2022Quote: ... total genomic DNA from each clone was extracted from 2mL of an overnight culture using the GenElute Bacterial Genomic DNA kit (Sigma-Aldrich) and the quality and integrity of the DNA was assessed on a 1% agarose gel and the concentrations were determined fluorometrically using Quant-iT™ PicoGreen® dsDNA Reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... equal number of DB-VOs and control ND-VOs were labelled by LuminiCell Tracker 670-Cell Labeling Kit (SCT011, Sigma-Aldrich), 0.8nm for 12 hours in Stempro34 complete medium ...
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bioRxiv - Plant Biology 2024Quote: RNA of aerial and subterranean fruit valves (stage 16-17a and 17ab) was extracted from three biological replicates using SpectrumTM Plant Total RNA kit (Sigma-Aldrich). For long read sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was immediately extracted from pooled samples after grinding using a Spectrum™ Plant Total RNA Kit from Sigma-Aldrich (STRN250) and an On-Column DNase I Digestion Set from Sigma-Aldrich (DNASE70-1SET ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was assayed between 20-24 hours post-treatment for lactate dehydrogenase (LDH) activity as a marker of cell death using a commercially available kit (Tox7, Sigma-Aldrich) as previously described (Aras et al. ...
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bioRxiv - Neuroscience 2024Quote: ... Debris was removed with density gradient centrifugation using Nuclei PURE 2 M sucrose cushion solution and Nuclei PURE sucrose cushion buffer from the Nuclei PURE prep isolation kit (no. NUC201-1KT, Sigma Aldrich). Nuclei PURE 2 M sucrose cushion solution and Nuclei PURE sucrose cushion buffer were first mixed in a 9:1 ratio ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA extraction with an on-column DNAse treatment was performed with the GenElute Mammalian Total RNA Miniprep Kit (Sigma Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR sex genotyping reactions were performed in a final volume of 25 μl with the following reagents and volumes per sample: 12.5 μl KAPA2G Fast HotStart DNA Polymerase (5 U/μL) from the KAPA2G Fast HotStart PCR Kit (Sigma-Aldrich, KK5601), 1 μl of 12.5 μM Ube forward primer and 1 μl of 12.5 μM Ube reserve primer ...
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bioRxiv - Genetics 2024Quote: ... digestion at 37 ᵒC for 20 minutes and used directly for bisulfite treatment using the Imprint DNA Modification Kit (Sigma MOD50) according to the manufacturer’s 2-step modification procedure ...
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bioRxiv - Bioengineering 2023Quote: ... This cell inoculated hydrogel precursor was injected into central tissue lane of MFOC devices and incubated for 30 minutes prior to filling the side channels and media reservoirs with endothelial cell growth medium (VEGF kit) containing 25 µg/mL aprotinin (Sigma-Aldrich) to inhibit fibrin degradation.
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bioRxiv - Neuroscience 2023Quote: DNA from larval tails and juvenile and adult tail fins was extracted using the Extract-N-Amp Tissue PCR Kit (Millipore Sigma). After verifying SNP location with Sanger sequencing at Azenta Life Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... apoptotic cell death in 1-day old zebrafish whole-mounts was detected according to a modification of the ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Merck Millipore) protocol ...
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bioRxiv - Biochemistry 2024Quote: Genomic DNA (gDNA) was extracted from cell pellets using the Sigma GenElute Mammalian gDNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO). DNA amplicons for Illumina sequencing were produced from the gDNA using a previously described semi-nested polymerase chain reaction (PCR ...
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bioRxiv - Pathology 2024Quote: The content of human IgG was quantitated both in the sera and aortic (tissue) homogenates from rats treated with chP3R99 or hR3 mAbs by a commercial ELISA kit (Sigma-Aldrich). Similarly ...
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bioRxiv - Physiology 2023Quote: ... Samples were stored at −20°C and insulin secretion was measured using a mouse insulin radioimmunoassay kit (Millipore Cat# HI-14K).
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted from 2 ml of overnight culture from each time point using the GenElute™ Bacterial Genomic DNA kit (Sigma) and 10 ng analysed by qPCR using a Light Cycler 480 real-time PCR system (Roche ...
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bioRxiv - Bioengineering 2024Quote: ... very low- and low-density lipoprotein [(V)LDL] were separated from the plasma above using a HDL and LDL/VLDL Quantitation Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... R-domain phosphorylation sites and interacting residues mutants were generated by site-directed mutagenesis using a Q5-New England Biolabs PCR mutagenesis kit together with primers synthesized from Millipore sigma and verified by sequencing (Elim Biopharmaceuticals ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were confirmed to be free of mycoplasma contamination (VenorTMGeM Mycoplasma Detection Kit, PCR-based, Sigma Aldrich Cat #MP0025). Cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... in FibroGRO™ Complete Media Kit supplemented with bFGF (75 ng/mL) and 2% of foetal bovine serum (FBS, Sigma-Aldrich). Only iPSC-cFbs with a passage number ≤ 10 (P10 ...
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bioRxiv - Cell Biology 2024Quote: ... live tachyzoites prepared as mentioned above were incubated with the lipid dye PKH26 to label the plasma membrane using the Cell Linker kit (041M0852, Sigma Aldrich) in 0.5 mL final volume following the manufacturer protocol ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
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bioRxiv - Molecular Biology 2023Quote: ... the mouse anti-DCP1A antibody was covalently conjugated to a 488 fluorophore using the Mix-n-Stain CF488A Antibody labeling kit (Sigma, MX488AS100) and used together with the rabbit polyclonal anti-ORF1p antibody covalently conjugated to a 647 fluorophore with the Mix-n-Stain CF647 Antibody labeling kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The ApopTag Peroxidase In Situ Apoptosis Detection Kit was used for Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (Sigma Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from ~30 mg of abaxial epidermis or the remaining leaf tissue using the Spectrum Plant Total RNA kit (Sigma-Aldrich) including on-column DNase I digestion (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were then washed 2x in PBS before performing proximity ligation reaction using the Duolink In Situ Red Starter kit (Sigma Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA from mouse liver or striatum (see below) was extracted using GenElute Mammalian Genomic DNA Miniprep Kit (Millipore Sigma #G1N350).
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Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging responsebioRxiv - Plant Biology 2023Quote: ... The tissue was ground in liquid nitrogen and total RNA was extracted using Spectrum™ Plant Total RNA kit (Sigma-Aldrich). For each condition (waterlogged or untreated) ...
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Alkaline-phosphatase (Alk-Phos) positive cells were assessed on day 18 in culture using Leukocyte Alkaline Phosphatase Kit (Sigma; cat#86R) following manufacturer instructions.
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human CD56 Positive Selection Kit II) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Systems Biology 2023Quote: The cell toxicity of the drugs was determined using Cell Counting Kit-8 (CCK-8) (Catalog No.: 96992-100TESTS-F, Sigma-Aldrich) according to the manufacturer’s directions ...
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bioRxiv - Plant Biology 2023Quote: ... ∼50 mg of tissue was ground under liquid nitrogen and the total RNA was ex-tracted using the Spectrum Plant Total RNA Kit (Sigma-Aldrich) following the manufac-turer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal deletion mutants were verified by amplification of the genomic region from isolated genomic DNA (GeneElute Bacterial Genomic DNA Kit, Sigma Aldrich) and by whole genome sequencing (MicrobesNG/Plasmidsauraus) ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were regularly tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO, CA).
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bioRxiv - Genetics 2023Quote: ... The osteoclasts were fixed and stained with tartrate-resistant acid phosphatase (TRAP)-positive cells based on a leukocyte acid phosphatase kit (cat no 387A; Sigma; USA) according to the manufacturer’s instructions ...