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Citations for Millipore Sigma :
5451 - 5500 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Downregulation of PAK6 expression was performed utilizing an shRNA against human PAK6 (sh-1944, Sigma). Western blot in SH-SY5Y cell extracts was performed with anti-PAK6 (Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... 2-3-week-old plants were treated with 150µM DCMU (D2425-100G, Sigma), kept for 1 hour in the dark and then exposed to high light (HL ...
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bioRxiv - Biophysics 2021Quote: ... Ultrapure water from a Direct-Q 3 UV water purification system (Millipore, USA) was used for all experiments.
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bioRxiv - Cell Biology 2020Quote: ... and 3 μM CHIR99021 on 0.1% gelatin (Catalog No. ES-006-B, Millipore)-coated plates (for the 2i condition) ...
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bioRxiv - Cell Biology 2019Quote: ... Fractions were analyzed by immunoblotting using an antibody to PC1/3 (Sigma, #SAB1100415). Band intensity was quantified using FIJI and plotted against the sucrose concentration of the fraction.
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bioRxiv - Cell Biology 2019Quote: ... all coverslips were incubated with a 5% 3-aminopropyltrimethoxysilane solution (281778, Sigma-Aldrich) in water for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: Cells were plated at 3*105 cells/well in fibronectin-treated (EMD Millipore) 96-well glass-bottom plates (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 1 ml of culture was supplemented with 3 mg/ml propidium iodide (Sigma; stock 1 mg/ml in phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000037282 (shRNA Trim39#2) and TRCN0000438509 (shRNA Trim39#3) were from Sigma-Aldrich. Lentiviral particles were produced as previously described (Iréna Lassot et al. ...
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bioRxiv - Cell Biology 2020Quote: ... bafilomycin A1 and 3-methyladenine were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich), an equivalent volume of DMSO was added to control samples.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The plates were washed 3 times with 0.05% Tween-20 (P2287, Sigma-Aldrich) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.4) containing 1 mM PMSF and phosphatase inhibitor cocktail 3 (Sigma-Aldrich). Cells were scraped off ...
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bioRxiv - Developmental Biology 2021Quote: ... The non-specific binding of antibodies was blocked with 3% BSA (Sigma-Aldrich) for 45 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... (1.5% CMC (3% (w/v) carboxymethylcellulose solution in sterile distilled water (Sigma C4888)) ...
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bioRxiv - Cell Biology 2019Quote: ... and 2 mg/ml N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) (Sigma, 03450) in 50 mM HEPES were added on top of the hydrogels and incubated for 30 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% DMSO and 300 μM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS; Sigma) dissolved in microtubule stabilization buffer specific for algae (MTB ...
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bioRxiv - Developmental Biology 2020Quote: ... 3-5 colonies were picked and incubated in 2mL Terrific Broth (T0918, Sigma) containing 100µg/mL Ampicillin for 8 hours ...
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bioRxiv - Genomics 2020Quote: ... before addition of 500 μM indole-3-acetic acid solution (“auxin”, Sigma-Aldrich) for different times to induce RPB1 degradation ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with 100 μg/ml of 3×FLAG peptide (Sigma). The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min and were blocked with 3% bovine serum albumin (BSA; Sigma) for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] were acquired from Sigma (USA). Purified water was prepared with a Milli-Q purification system from Millipore (Milford ...
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bioRxiv - Molecular Biology 2021Quote: ... selected for 2-3 weeks with 1 µg/ml of puromycin (Sigma- Aldrich), and surviving cells were fixed with methanol and stained with Giemsa (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then washed with PBS containing 3% Bovine Serum Albumin (BSA, Sigma). Following MeTAFlow assay the cells were passed through a strainer cap to achieve a single cell suspension and immediately analyzed using BD FACS Aria Fusion SORP Cell Sorter ...
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bioRxiv - Cell Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl phosphate-toluoidine were from Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2022Quote: ... Elution was achieved by using 250 μg/ml 3 × Flag peptides (F4799, Sigma). The resulting eluates were resuspended in TRIzol for the following RNA extraction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All fish were euthanized with ethyl 3-aminobenzoate methanesulfonate (MS-222, Sigma-Aldrich), and then fixed in ethanol or RNAlater solution (Thermo Fisher) ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... they were washed and blocked with 3% Bovine Serum Albumin (BSA, Sigma-Aldrich) and 100 μM NiSO4 for 20 min ...
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bioRxiv - Genomics 2020Quote: ... To the XRN1-AID strain 0.2mM of auxin (3-Indoleacetic acid; Sigma-Aldrich) was added to the media 30 and 60 minutes before harvesting ...
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bioRxiv - Immunology 2021Quote: ... Spleens were gently meshed in FACs buffer (PBS containing 3% FCS from Sigma) using a 40μM cell strainer to prepare single cell suspensions ...
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bioRxiv - Genomics 2019Quote: ... thyroxine (0.4 µg/ml) and glucose (3 g/l) (all from Sigma-Aldrich).
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bioRxiv - Genetics 2019Quote: ... ChAT and Cleaved caspase 3 sections were then counterstained with DAPI (Sigma; 10236276001) and GFAP sections were counterstained with NeuroTrace™ 500/525 Green Fluorescent Nissl Stain (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... Samples were concentrated with 3 kDa centrifuge units (Millipore Sigma, St. Louis, MO), and protein concentration was measured using a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mg/ml N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) (Sigma-Aldrich, 03450) diluted in 50 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ...
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bioRxiv - Bioengineering 2021Quote: ... Passages were performed by a 3 min treatment of Dispase II (D4693, Sigma) at 37°C ...
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bioRxiv - Genetics 2020Quote: ... for 30min and were incubated with blocking solution [3% bovine serum albumin (Sigma), 5% donkey serum (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich) added fresh] or RIPA buffer [25 mM Tris pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... embryos were anesthetized with 500 μM tricaine (Ethyl 3-aminobenzoate methanesulfonate; Sigma-Aldrich), mounted in 3% methyl-cellulose on a glass slide and imaged on a Nikon SMZ25 stereomicroscope.
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bioRxiv - Plant Biology 2020Quote: ... proteins were eluted with 150 μg.ml-1 3× FLAG peptide (Sigma-Aldrich, F4799) in extraction buffer ...
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bioRxiv - Neuroscience 2020Quote: ... frogs were anesthetized in 0.25% ethyl 3-aminobenzoate methanesulfonate solution (MS222; Sigma-Aldrich); bags of ovaries were removed surgically from the anesthetized frogs ...
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bioRxiv - Neuroscience 2020Quote: ... 3% agar gel consisting of 10% concentration fluorescein dye (F2456, Sigma Aldrich Inc.) was prepared ...
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bioRxiv - Microbiology 2021Quote: ... 3 ml LB top-agar (LTA: 20g LB, Sigma; 7.5 g agar, Formedium) or BHI top-agar (BTA ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were then incubated with 3% (v/v) hydrogen peroxide solution (Sigma-Aldrich) for 20 min to block endo-peroxydase activity ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilization and blocking was performed with 3% Bovine Serum Albumin (BSA, Sigma-Aldrich), 0.1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... were resuspended in 25 µL of 3 M HCl in methanol (90964, Sigma), and incubated at 20 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... in which 3 mL of 1.3 mg/mL cold collagenase P (Sigma 11213865001) and 0.01 mg/mL DNAse (Sigma D5025 ...
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bioRxiv - Bioengineering 2021Quote: ... 3) the shielding oil consisted of 0.5% (v/v) Span-80 (Sigma-Aldrich) surfactant dispersed in mineral oil (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed samples were incubated with blocking solution composed of 3% BSA (Sigma-Aldrich) in PBS for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... 0.75 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) was added to culture medium for 2 h ...
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bioRxiv - Developmental Biology 2020Quote: ... TF induction was performed by adding 3 μg/mL of Doxycycline (Sigma, D9891) on day 2 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3 days on food containing 400 µM all-trans retinal (Sigma). The following genotypes were assayed ...