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Citations for Millipore Sigma :
501 - 550 of 7003 citations for ZAP70 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... baseline blood glucose was taken before recombinant human insulin (Sigma, USA) (0.5 U/kg – 1 U/kg ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human MMP-3 (SRP7783) was purchased from Sigma Aldrich (Germany).
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) for CSR to IgG1 ...
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bioRxiv - Cell Biology 2022Quote: ... 20 ng/ml recombinant human epidermal growth factor (hEGF, #E9644, Sigma), 0.25 ng/mL hydrocortisone (#H0888 ...
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bioRxiv - Bioengineering 2022Quote: ... and recombinant insulin human (≥ 98%) were all purchased from Sigma-Aldrich. Water (LC-MS grade) ...
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bioRxiv - Genomics 2022Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg/ml of recombinant human insulin (Sigma-Aldrich, 19278-5ML), 2 IU/ml heparin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) (L-I) ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant human Hepatocyte Growth Factor (HGF) was obtained from Sigma Aldrich Israel ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Systems Biology 2024Quote: ... and 500 U/mL recombinant leukemia inhibitory factor (LIF; Millipore, ESG1107) at 37 °C in 5% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Cell Biology 2023Quote: Human recombinant Cdk9/cyclin K and CDK9/cyclin T (Sigma Aldrich) were incubated with the substrate at 30°C for 30 minutes in reaction buffer (25 mM Tris-acetate (pH 7.9) ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μg/mL recombinant human albumin (Sigma-Aldrich, cat. no. A9731), and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: All recombinant proteins were expressed using pET-11a (Sigma #69436-3) based vectors in BL21(DE3 ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant full-length human active AAK (#14-511 Merck Millipore, Germany) were incubated either in the presence or the absence of 14nM of N-terminal GST-fused active HsMPS1/TTK (SignalChem ...
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bioRxiv - Cell Biology 2020Quote: The antibodies used in this study are as follows: alpha-tubulin (mouse mAb DMIA; T9026; Sigma), Arl13b (mouse mAb N295B/66 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were incubated with purified mAbs (0.1-2.0 µg/mL) diluted in 10% NGS (Sigma-Aldrich) / 0.1% saponin ...
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bioRxiv - Molecular Biology 2022Quote: ... The primary antibodies purchased from commercial sources are listed below: mouse anti-HA mAb (Sigma, USA), mouse anti-FLAG mAb (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... blots were incubated in a 1:10,000 dilution of anti-MBP antibody (EDM Millipore, #MAB 386) in 5% BSA in TBST overnight at 4C ...
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bioRxiv - Biochemistry 2021Quote: ... was initially subjected to affinity chromatography using a protein-A/anti-BSA mAB matrix (Sigma-Aldrich) to remove excess albumin (a heme-binding protein contaminant) ...
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bioRxiv - Neuroscience 2022Quote: ... Type 1 progenitors were identified by immunostaining for GFAP (mouse mAb anti-GFAP; #IF03L, Millipore Sigma) and SOX2 (rabbit polyclonal anti-SOX2 #5603 ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... pH 7.4 for affinity purification of the FLAG-tagged cHMM on M2 mAb-Sepharose beads (Sigma). Bound cHMM was eluted with 0.1 mg⋅mL−1 FLAG peptide (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Antibody binding was detected with goat anti-mouse IgG mAb conjugated with AlexaFluor 647 fluorochrome (Sigma). Sample acquisition was performed on a LSR Fortessa-X20 cytofluorimeter (BD biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse anti-b-actin mAb (clone AC-15; #A1978) was obtained from Sigma-Aldrich (MO, USA). Rabbit anti-GFP pAb was from MBL (Nagoya ...
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bioRxiv - Microbiology 2023Quote: ... HCoV-OC43 rN was additionally characterized by using mouse anti-OC43 N mAb (Sigma-Aldrich # MAB9013) followed by IRDye® 800CW Goat anti-Mouse IgG Secondary Ab (LI-COR # 926-32210).
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used were mouse mAb to MT1-MMP (1:500; clone LEM-2/15.8, Millipore), mAb GAPDH (1:10000 ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... Purified mAbs in PBS were then filtered using sterile 0.2-μm pore size filter devices (Millipore), and stored in aliquots at 4°C until future use.
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bioRxiv - Microbiology 2023Quote: ... incubated with mouse anti-HA mAb clone 12CA5 conjugated with protein G agarose beads (Sigma-Aldrich) for 16 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... using either 0.5 μg of recombinant histone H3 (Millipore, Cat. #14-411), 2 μg of chicken core histones (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Cell Biology 2020Quote: ... 14-3-3 antibody and active recombinant AMPK (α2β1γ1) were from Millipore. Precast Tris-Glycine polyacrylamide gels and 10X tris-glycine gel electrophoresis buffer were from BioRad ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Genetics 2020Quote: ... 0.5 U DNA recombinant Taq polymerase in buffer (pH= 8.0; Sigma, Germany), 1x PCR buffer (pH=8.7 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 ng/mL of recombinant basic fibroblast growth factor (Sigma-Aldrich). RAW 264.7 macrophages were cultured in complete media composed of high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM (1x ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Biochemistry 2021Quote: ... Bovine erythrocyte ubiquitin and recombinant hsNEDD8 were purchased from Sigma-Aldrich (U6253) and BostonBiochem (UL-812 ...
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bioRxiv - Microbiology 2020Quote: ... A set of recombinant RT standards (EMD Millipore, Molsheim, France; catalog# 382129) ranging from 5.12e3 nU/mL to 5.12e9 nU/mL was used to estimate the nU/mL of reverse transcriptase activity in the FIV stock.
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml of recombinant human leukemia inhibitory factor (LIF, EMD Millipore) and 1 mM Valproic acid (Wako) ...
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bioRxiv - Bioengineering 2020Quote: Recombinant human epidermal growth factor (EGF, 5 ng/mL; Sigma-Aldrich, E9644) was used as an invasion promoter and recombinant human nodal (250 ng/mL ...