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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and incubated in secondary antibodies (Supplemental Table 3) and DAPI (10g/ml, Sigma,) diluted in 10% NDS in 0.1% Triton-PBS for 90 minutes at r.t ...
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bioRxiv - Microbiology 2019Quote: ... the cells were incubated with a rabbit anti-Gal-3 antibody (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Aggregates were incubated with primary antibodies in blocking buffer (3% BSA (Sigma, A9647) and 0.3% Triton X-100 in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... pre-incubated for 3-7 h with rabbit anti-FLAG antibody (Sigma-Aldrich) at 4°C ...
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bioRxiv - Genetics 2022Quote: ... or 1:3000-diluted antibody against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (Sigma-Aldrich). After repeated washings ...
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bioRxiv - Immunology 2022Quote: ... incubated overnight with 3 μg of mouse anti-FLAG antibody (clone M2; Sigma), and then for 10 minutes with 2 μg of rat anti-Ms IgG (clone P3.6.2.B.1 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were anti-Acetyl Tubulin (Lys40) (1:1000, 3 hrs; Sigma ABT24) and anti-MIC2 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... chromatin was immunoprecipitated using 3 μL anti-FLAG antibody (F1804, Sigma-Aldrich, USA) or Anti-IgG antibody (A9044 ...
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bioRxiv - Biophysics 2023Quote: ... The cleared supernatant was then incubated with 3 µg anti-myc antibody (SIGMA M4439 monoclonal anti c-myc or Abcam ab206486 rat mAb to myc tag (9E10) ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by incubation with primary antibody in 3% bovine serum albumin (BSA) (Sigma) in PBST at 4°C overnight ...
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bioRxiv - Biophysics 2022Quote: ... The primary antibody (1:100 Anti-Tau [3-repeat isoform RD3] Antibody, clone 8E6/C11, 05-803, Sigma-Aldrich and 1:100 Anti-Tau [4-repeat isoform RD4] Antibody ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Molecular Biology 2019Quote: ... 4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk ...
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bioRxiv - Cell Biology 2020Quote: Total protein cell lysates were obtained using RIPA buffer containing protease inhibitor cocktail (11697498001, Sigma-Aldrich), phosphates inhibitors (A32957 ...
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bioRxiv - Microbiology 2021Quote: ... enabling formation of protein-containing polyacrylamide plugs upon polymerization using ammonium persulphate and TEMED (Sigma-Aldrich). Gel plugs were then diced by spinning at 14,000 xg through plastic mesh ...
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bioRxiv - Immunology 2019Quote: ... We prepared protein lysates by scraping the cells in cold lysis buffer containing 1% Igepal (Sigma) and CompleteTM protease inhibitor cocktail and preclearing by centrifugation at 14,000 g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Bottles were filled with either protein-containing solution (4% casein, sodium salt from bovine milk, Sigma #C8654 ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant containing proteins was collected after centrifugation and incubated in benzonase (EMD Millipore/Novagen, Country) at 37 °C for 30 min to shear chromatin ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant containing proteins was collected after centrifugation and incubated in benzonase (EMD Millipore/Novagen, Country) at 37 °C for 30 min to shear chromatin ...
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bioRxiv - Microbiology 2021Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... the protein-containing fractions were dialyzed in a cellulose membrane tube (14 kDa MWCO, Merck-Millipore), first ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants containing lentivirus were filtered through a low-protein binding syringe filter (0.45 μm, Millipore #SLHP033RS) before transductions ...
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bioRxiv - Microbiology 2021Quote: ... the protein was eluted with 6 ml buffer B containing 15 mM d-desthiobiotin (Sigma Aldrich). Purification of GyrA-N-Strep and GyrB-C-Strep were conducted in the same way using an adjusted buffer Bgyr for cell disruption and purification (buffer Bgyr ...
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bioRxiv - Cancer Biology 2021Quote: ... Fractions containing the defensin protein were concentrated using a Minitan II ultrafiltration system (Millipore, Bedford, MA) with a 3-kD cutoff membrane and dialyzed against 10 mM Tris ...
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bioRxiv - Plant Biology 2022Quote: ... bound proteins were eluted using buffer A containing 500ng/μL 3X FLAG® peptide (Sigma; F4799) for followed SDS-PAGE.
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bioRxiv - Molecular Biology 2023Quote: ... the instrument was calibrated using a protein standard containing 10 nM β-amylase (Sigma Aldrich A8781) and 3 nM Thyroglobulin (Sigma-Aldrich 609310) ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Forty micrograms of protein samples were denatured in reducing loading buffer containing β-mercaptoethanol (M3148, Sigma) and 0.0002% bromophenol blue at 95°C for 5 minutes and separated in pre-casted 12% or 7.5% Tris-Glycine polyacrylamide gel using the Criterion cell system (Biorad) ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected with primary antibodies to FLAG M2 (Sigma, A8592), HA 3F10 (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore, Massachussets, United States).
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Antibodies were enriched from antiserum using protein A-agarose (Sigma-Aldrich). Affinity purification of TaBGC1-specific antibodies from the antiserum was performed with a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma), eGFP fusion protein levels monitored using anti-GFP antibody (ab290 – abcam ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction and Western blot using anti-puromycin antibody (Merck Millipore) was performed as described before.
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bioRxiv - Plant Biology 2022Quote: ... proteins were detected with alkaline phosphatase-conjugated antibody (Sigma-Aldrich A3687). Primary antibodies used were ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse GAPDH monoclonal antibody for protein loading controls (Sigma, G8795). Anti-mouse IRDye680RD and anti-rabbit IRDye800CW (Licor ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were recovered by binding to protein A/G agarose (Millipore), and the eluted DNA fragments were used directly for qPCR or subjected to high-throughput sequencing (ChIP-Seq ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against the following proteins were used: FLAG (Sigma Aldrich F1804), Streptavidin-Alexa Fluor 488 (Life S32354) ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitinated proteins were detected using an anti-FLAG antibody (F7425, Sigma), HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected by a monoclonal anti-FLAG antibody (Sigma, USA) or a polyclonal anti-GFP antibody (GenScript ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Genetics 2023Quote: ... PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich) and separated by SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were immunoprecipitated by the addition of 3 µl of rabbit anti-FGFR3 (Sigma-Aldrich, F0425)/500 µg protein with protein A-agarose (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Cell Biology 2019Quote: ... Silicon wafers were silanized for protein conjugation by incubation with 3-glycidoxy-propyl-dimethoxy-methylsilane (Sigma) (0.045 % in 100 % EtOH ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein 5-HT2C or HCA2 (3 μg) was immobilized on nickel agarose beads (Sigma) in the incubation buffer containing 50 mM HEPES ...