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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Recombinant Human Aldehyde Dehydrogenase 1 Family Member A3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1 IU/mL insulin and 10 ng/mL human recombinant epidermal growth factor (EGF) (all from Sigma-Aldrich). TKCC10 cells were grown in a 1:1 mixture of M199 medium and Ham’s F12 medium ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with medium containing 10 µg/mL recombinant human adiponectin (SRP4901, Sigma-Aldrich, in sterile water), a quantity equivalent to typical adiponectin levels in lean women (Ding et al. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
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bioRxiv - Neuroscience 2019Quote: ... Human Nuclei (1:300, Millipore), Human GFAP (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Human nuclei (1:200, Millipore), Iba1 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... human nuclei (1:1000, Millipore), Islet1 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant protein was induced with 1 mM IPTG (Sigma – Aldrich) and the culture was transferred to 25°C and 250 rpm for 5 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µl recombinant RNase inhibitor (1 U/µl, Sigma-Aldrich) was added to 390 µl internal solution to increase RNA yield ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaved His-tag and His-tag-fused TEV protease were removed using a HIS-Select® Nickel Affinity Gel (Sigma-Aldrich) column ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant MEK and recombinant ERK were purchased from Sigma. Indicated amounts of recombinant GST-HSF-1 (0.1–0.4 mg ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Microbiology 2021Quote: Dehydrogenase activity was measured using the Cell Counting Kit-8 (Sigma-Aldrich). Twenty-µL WST-8 reagent was added to each well of 96-well plates already containing 200 µL cell culture medium per well and incubated for 1-2 hours at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... 0.04 U/μl pyruvate kinase/ 0.05 U/μl lactic dehydrogenase (Sigma-Aldrich) and 5 mM ATP ...
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bioRxiv - Cancer Biology 2022Quote: ... Ammonia was then quantified using the L-Glutamate Dehydrogenase ammonia kit (Sigma). Ammonia was converted to indophenol using the Berthelot reaction ...
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bioRxiv - Immunology 2022Quote: ... Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody clone 6C5 (Merck Millipore, MAB374) was used as control antibody ...
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bioRxiv - Biochemistry 2019Quote: ... 100 units/mL lactate dehydrogenase and 80 units/mL pyruvate kinase (Sigma) in buffer composed of 50 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... and L-Glutamate dehydrogenase from bovine liver (GLDH, Sigma, Saint-Louis, USA) were diluted to 1mg/mL in GibcoTM phosphate buffer saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... we measured the level of lactate dehydrogenase using a commercial kit (Sigma) (29) ...
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bioRxiv - Microbiology 2023Quote: ... we measured the level of lactate dehydrogenase using a commercial kit (Sigma) (50) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02 mg/mL lactate dehydrogenase from rabbit muscle (Sigma-Aldrich, Schnelldorf, Germany), 0.05 mg/mL pyruvate kinase from rabbit muscle (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... and 0.36 U glucose-6-phosphate dehydrogenase (G6PDH) (G7877; Sigma-Aldrich/Merck) to each tube ...
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bioRxiv - Biochemistry 2024Quote: ... 0.04 U/μl pyruvate kinase/0.05 U/μl lactic dehydrogenase (Sigma-Aldrich) and 5 mM ATP ...
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bioRxiv - Biochemistry 2024Quote: ... PLA2G15 used in the reactions was obtained by overexpressing C-terminally FLAG-tagged human PLA2G15 in HEK293FT cells and affinity-isolation using anti-FLAG magnetic beads (Sigma-Aldrich #M8823).
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bioRxiv - Physiology 2022Quote: ... and following the best approaches for optimizing activity (Moyes et al., 2021): Solution A (4 units mL-1 lactate dehydrogenase (L2500, Sigma), 5 units mL-1 pyruvate kinase (P1506 ...
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bioRxiv - Microbiology 2023Quote: ... 2012) using the reaction buffer (bicine-KOH 20 mM, pH=9.0 and 1 U/mL alcohol dehydrogenase, Sigma Aldrich A7011). The assay was carried out at 340 nm ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2023Quote: ... the FSH-cultured follicles were then incubated with or without the PPP family phosphatase inhibitor cantharidin (C7632, Sigma-Aldrich) or the PKA inhibitor Rp-8-CPT- cAMPS (C011-05 ...
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bioRxiv - Genomics 2022Quote: ... FITC-tagged NeuN antibody (Sigma, MAB377) was prepared following manufacturer’s instructions (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed sections were treated sequentially with 0.3% hydrogen peroxide to suppress endogenous peroxidases and 1% sodium borohydride to reduce exposed aldehydes and improve background staining before incubating overnight in a tyrosine hydroxylase antibody solution (Millipore Cat# MAB318 ...
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bioRxiv - Cell Biology 2024Quote: ... PFA fixed samples on coverslips were successively incubated in 50 mM NH4Cl in PBS (10 min) to quench the free aldehyde groups and in BSA (2% w/v, Sigma)-containing PBS to prevent non-specific binding of the subsequently used antibodies ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Fluorescence staining was done by GC-specific fluorochrome Chromomycin A3 (CMA3) and AT-specific fluorochrome DAPI (both Sigma-Aldrich), following Mayr et al ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).