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Citations for Millipore Sigma :
501 - 550 of 651 citations for Ovalbumin Atto 594 conjugate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with the secondary anti-mouse IgG-HRP conjugate (GENA931, Sigma Aldrich, 1:10,000). Signals were detected using SuperSignal West Dura Extended Duration Substrate (Pierce ...
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bioRxiv - Immunology 2024Quote: ... followed by the addition of a goat anti-human IgG Fc-alkaline phosphatase conjugate (Sigma). The reaction was developed using paranitrophenylphosphate substrate (1 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... the streptavidin-HRP conjugates were either subjected to SigmaFast 3,3’-diaminobenzidine (DAB) staining (Sigma-Aldrich) or TSA with Alexa Fluor 488 (TSA kit no.22 ...
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bioRxiv - Neuroscience 2024Quote: ... the tissues were stained with anti-GFAP Cy3 conjugate (Millipore Sigma, MAB3402C3, dilution 1/50) for 30 min RT ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibody was an anti-rabbit IgG (whole molecule) peroxidase conjugate (Sigma-Aldrich, USA), also diluted 1:1000 ...
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bioRxiv - Biophysics 2024Quote: ... Albumin–fluorescein isothiocyanate conjugate (FITC-albumin) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... were first coated with streptavidin and next with Concanavalin A-biotin conjugate (Sigma Aldrich, C2272) during a 2-hour incubation at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... rehydrated in a graded ethanol series and stained for 10 minutes with Atto 465-carboxcylic acid (8 uM; Sigma-Aldrich, St. Louis, MO), Atto 465-p (8 μM) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2022Quote: Stock solutions of VSV-SARS-CoV-2 and its variants at a concentration of ∼150 µg/ml viral RNA were conjugated with Atto 565-NHS ester (Sigma-Aldrich, cat. 72464) as previously described (41) ...
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bioRxiv - Neuroscience 2024Quote: ... each sample was separated on 5-20% gradient e-PAGEL mini gels (ATTO Corporation, Tokyo, Japan) and transferred to polyvinylidene fluoride membranes (Merck Millipore, Billerica, MA, USA). The membranes were incubated for 30 min with blocking buffer (Nacalai Tesque) ...
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bioRxiv - Biochemistry 2024Quote: Approximately 0.5 mL of purified MBP-FUS protein was fluorescently labeled with amine-reactive Atto 488-NHS ester (Sigma-Aldrich 41698-1MG-F). After resuspending the reactive dye in anhydrous DMSO to a stock concentration of 10 mM ...
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bioRxiv - Biophysics 2021Quote: ... After staining with secondary antibodies (conjugated with Alexa Fluor 488 or 594; Millipore) and Hoechst 33342 (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2024Quote: ... we incubated each donor (D) or acceptor (A) oligomer with a 20x molar excess of the appropriate Atto-NHS ester dye (Sigma-Aldrich 92835 and 18373) in a fresh sodium bicarbonate buffer in 18.2 MΩ double-deionized water ...
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bioRxiv - Neuroscience 2023Quote: ... then washed three times in PBT and incubated in secondary antibody mix (goat anti-chicken Alexa Fluor 488, Jackson Immunoresearch #103-545-155, 1:200, goat anti-rabbit ATTO 647N, Sigma #40839, 1:100) for 24 - 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... PIP2 was labeled with PIP2 antibody (Echelon, Z-P045) conjugated with Cy3B NHS ester or Atto 488 NHS ester (Sigma-Aldrich, 41698-1MG-F). Following the labeling ...
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bioRxiv - Cell Biology 2020Quote: ... Lectin staining was done using Lectin from Arachis hypogaea FITC Conjugate (IF, 1:1000, Sigma: L7381).
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Efficacy of recombinant NP-M1 and NP-M1-CRT DNA vaccines against Influenza A viruses in mice C57/BL6bioRxiv - Molecular Biology 2021Quote: ... After washing 100 μ l of 1:10000 rabbit anti mouse IgG-HRP conjugates (Sigma-Aldrich), the peptide were added to the wells and incubated for two additional hours at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... The hybridized antibody-oligonucleotide conjugates were purified using an Amicon 50K MWCO column (Millipore cat. UFC505096) according to the manufacturer's instructions to remove excess free oligonucleotides.
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bioRxiv - Microbiology 2021Quote: ... Cells were further stained with the secondary antibody anti-rabbit HRP conjugate (Sigma; 1:3000 dilution) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with the anti-rabbit IgG Peroxidase conjugate secondary antibody (1:3000, Sigma-Aldrich) and immunocomplexes were visualized by enhanced chemiluminescence (Clarity™ Western ECL Substrate ...
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bioRxiv - Cell Biology 2020Quote: The mixture was incubated with Dibenzocyclooctyne-PEG4-biotin conjugate (Sigma-Aldrich #760749; 50 μM final concentration) in a reaction volume of 100 μl for 1 h on a rotator in slow motion (9 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... then transferred to secondary antibody solution (donkey anti-chicken IgG CF488 A conjugate (Sigma, 1:500), donkey anti-rabbit IgG CF568 conjugate (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... followed by incubation with primary antibodies against Strep Tag II Antibody HRP conjugate (Sigma, 1:5000), anti-FLAG Antibody (Sigma F7425 ...
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bioRxiv - Immunology 2023Quote: ... 0.1 % Tween20 and incubated with 100 μl of goat anti-mouse IgG alkaline phosphatase conjugate (Sigma) (1/5000 dilution in PBS-0.5 mM EDTA ...
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bioRxiv - Bioengineering 2022Quote: ... calpain substrate peptide or conjugates were incubated with 26.6 nM human calpain-1 (Sigma-Aldrich, C6108) in 50 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid (HEPES) ...
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bioRxiv - Cell Biology 2022Quote: ... and conjugate antibodies were all diluted using the blocking solution (10% goat serum in PBS, Sigma). The primary antibodies used were γMLC2 rabbit Ab 1:100 (3672 ...
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bioRxiv - Microbiology 2023Quote: ... followed by 30 minutes incubation of secondary antibody HRP conjugate rabbit anti-goat IgG (Millipore Sigma) diluted 1:500 in dilution buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and goat anti-mouse IgG antibody HPR conjugate (1:10,000 EMD Millipore, 12-348; 12-349). Western blot detection was done with ChemiDoc XRS+ System-Bio-Rad and quantified using ImageJ software.
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bioRxiv - Neuroscience 2024Quote: ... Antibody-ssDNA conjugates were purified into PBS using Amicon Ultra centrifugal filters (10 kDa, EMD Millipore). Amino-modified phalloidin (Bachem ...
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bioRxiv - Physiology 2020Quote: The EdU Cell Proliferation Assay Kit (EdU-594, EMD Millipore Corp, USA, 17-10527) was used to measure cell proliferation ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated overnight with lectin from Ulex europaeus Alexa Fluor 594 (73873, Sigma) (For human samples ...
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bioRxiv - Molecular Biology 2024Quote: ... After staining with secondary antibodies (conjugated with either Alexa Fluor 488 or 594; Millipore) and Hoechst 33342 (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... The following secondary antibodies were used in this study: goat anti-rabbit IgG-AP conjugate (Sigma Aldrich) (dilution 1:6,000 in 5% w/v skimmed milk/TBS-T) ...
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bioRxiv - Molecular Biology 2020Quote: ... which in turn was detected using 1:5000 goat anti-mouse IgG alkaline phosphatase conjugate (Sigma, A3562).
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bioRxiv - Cell Biology 2022Quote: ... The membranes were subsequently incubated with peroxidase-conjugated secondary antibodies (anti-mouse IgG, HRP conjugate, EMD Millipore, AP160P ...
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bioRxiv - Biochemistry 2021Quote: ... Detection of HRP conjugates was carried out with 3,3′,5,5′-tetramethybenzidine (TMB/E, Millipore, Billerica, MA, USA). Detection of AP conjugated antibodies was carried out with SIGMAFAST p-nitrophenyl phosphate tablets (Sigma-N2770).
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bioRxiv - Immunology 2020Quote: ... This was followed by detection with a goat polyclonal anti-mouse IgG HRP-conjugate (1/1000; Millipore). For all ELISAs ...
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bioRxiv - Microbiology 2023Quote: ... The following secondary antibodies were used in this study: goat anti-mouse IgG-AP conjugate (Sigma Aldrich) (dilution 1:6,000 in 5% w/v skimmed milk/TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in secondary antibody or rabbit anti-NeuN (AlexaFluor 488 conjugate, 1:100, ABN78A4, Millipore, Billerica, MA), and DAPI (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated with an IB4-biotin conjugate (1:200, Sigma-Aldrich, Burlington, MA, USA, L2140) prepared in 5% normal goat serum (NGS)/PBST overnight at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were washed with TBS-T and incubated with goat anti-mouse IgG-peroxidase conjugate (Sigma DC02L) for 1 h at room temperature and washed again ...
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bioRxiv - Bioengineering 2022Quote: ... clone ELK3-51 Cyanine 3 conjugate mouse monoclonal antibody (5μg/mL in 1% BSA/PBS; Sigma-Aldrich). Imaging was done with the EVOS™ Light Cube ...
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bioRxiv - Genomics 2022Quote: ... firstly with anti-DIG2 (sheep anti-mouse Ig digoxigenin conjugate, 1:200, Merck Millipore, Billerica, MA, USA) and antistreptavidin (1:25 ...
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bioRxiv - Immunology 2024Quote: ... This was followed by detection with a goat polyclonal anti-mouse IgG HRP-conjugate (1/1000; Millipore). For all ELISAs ...
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bioRxiv - Molecular Biology 2024Quote: ... EU-labeling click reaction solution was made using 0.25M conjugated-azide (biotin: Vector labs; AF488-conjugate: Sigma), 0.5M CuSO4 ...
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TRPA1 block protects against loss of white matter function during ischaemia in the mouse optic nervebioRxiv - Neuroscience 2021Quote: ... and 0.05 Alexa Fluor 594 pH set to 7.15 with K-OH (all from Sigma). For Ca2+ imaging experiments ...
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bioRxiv - Developmental Biology 2024Quote: ... and the second antibody Goat anti-Mouse IgG(H+L) Alexa Fluor 594 (Sigma, SAB4600105) was added (diluted with PBTN at 1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins were detected by western blotting using either Anti-FLAG M2 monoclonal antibody-peroxidase conjugate (Sigma, A8592) at a dilution of 1:10000 ...