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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for FAS Associated Death Domain Protein FADD Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Equal amounts of SG core was subject to immunoprecipitation using anti-G3BP1 antibody (1ug) and isotype specific control antibody and protein A-sepharose beads (Sigma-Aldrich). Samples were incubated at 4⁰C overnight on a rotator and immune complexes recovered by centrifugation and five washes in buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Molecular Biology 2022Quote: ... the accumulated lysates were incubated with protein A/G magnetic beads (Thermo-Fisher) pre-coupled with anti-Flag antibody (M2 monoclonal antibody, Sigma-Aldrich) for 2 h at 4°C with rotation ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound samples were successively incubated with appropriate amount of primary antibodies against protein-of-interest (CTCF antibody: Millipore, 07-729) in 50µL of CUT&RUN antibody buffer for overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... either 1 tablet Roche cOmplete protease inhibitor cocktail (#11836145001) per 25 ml of FA lysis buffer or 30 µl aprotinin (Sigma-Aldrich: #A6279), 1 µl leupeptin (Sigma-Aldrich #L2884 ...
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bioRxiv - Biochemistry 2019Quote: The DNA construct coding for the motor domain of human kinesin-5 (Eg5; residues 1–368) was cloned into a modified pET16b (Novagen) vector for bacterial expression to achieve an N-terminally 6x His tag and TEV cleavage sequence ...
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bioRxiv - Biochemistry 2020Quote: A synthetic DNA fragment encoding the MTase domain of Arabidopsis thaliana DRM2 (residues 270-626) was cloned into pRSFDuet-1 vector (Novagen), preceded by an N-terminal His6-SUMO tag ...
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bioRxiv - Biochemistry 2022Quote: ... The protein complexes were eluted by on-column cleavage of MyoB neck domain using TEV and subsequently concentrated using a centrifugal concentrator (Millipore). The samples were analyzed using SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were diluted to a final concentration of 50 mM NaCl and then concentrated on Amicon centrifugal filters 30K for FHL and 10K for LIM domains (Millipore). The proteins’ purities and identities were confirmed by SDS-PAGE and mass spectrometry ...
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bioRxiv - Biochemistry 2021Quote: ... and 25 ng/μl E3 (HUWE1, catalytic domain, aa 3228-4374/1147 aa) were incubated with 0.3 μg/μl Ub (Sigma-Aldrich) and 11 ng/μl H1.2/H1.2 KxAc in 2 mM ATP ...
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bioRxiv - Biochemistry 2022Quote: The Sso2 sequence excluding only its C-terminal transmembrane domain (aa1-270) and the Sec3 PH domain sequence (aa75-260) were both sub-cloned into the pET-15b vector (Novagen) between the NdeI and BamHI sites ...
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bioRxiv - Biophysics 2020Quote: ... SEPT8G and SEPT11G) were performed by inserting the CDS for the corresponding G domains into the bicistronic expression vector pETDuet-1 (Novagen). The G domain of SEPT7 (SEPT7G ...
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bioRxiv - Biochemistry 2022Quote: ... The YopH tyrosine phosphatase expression vector pESU009 was generated by cloning the YopH phosphatase domain (residues 177-468) into pACYCDuet-1 (Novagen) under transcriptional control of an attenuated tac promoter.37 All constructs were confirmed by DNA sequencing.
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bioRxiv - Molecular Biology 2024Quote: cDNAs of BTB domains were PCR-amplified using corresponding primers (Supplementary Table S1) and cloned into modified pET32a(+) vector (Novagen) encoding TEV protease cleavage site after 6xHis-tag and Thioredoxin ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... to visualize FA formation and α-smooth muscle actin (α-SMA, mouse monoclonal anti-α-SMA clone 1A4, Sigma-Aldrich, A2547, 1:400). Hydrogels were washed three times using PBS and incubated with secondary antibodies (AlexaFluor 488 goat anti-rabbit IgG or AlexaFluor 555 goat anti-mouse IgG ...
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bioRxiv - Cell Biology 2022Quote: ... All iBAT pieces from one mouse were transferred into a well of a 12-well dish containing 0.5 ml high-glucose DMEM supplemented with 2% FA-free BSA (A6003, Sigma-Aldrich, St. Louis, MO) and 0.4 µCi [14C(U)]-D-glucose (ARC0122 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and formic acid (FA) were purchased from Biosolve (Valkenswaard, The Netherlands) and water was purified using a Milli-Q plus system (Millipore, Amsterdam, The Netherlands). Bovine plasma was obtained from Biowest (Amsterdam ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies against cytoskeletal proteins (vimentin, actin and β-tubulin were from Sigma (St.Louis, MO, USA). The mitochondrial marker MitoTracker® ...
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bioRxiv - Microbiology 2019Quote: ... Immunoblotting was performed with anti-Protein A mouse monoclonal primary antibody (SPA-27, Sigma Aldrich) or anti-LukS-PV mouse monoclonal primary antibody (IBT) ...
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bioRxiv - Plant Biology 2019Quote: ... Immunoblots of targeted proteins were visualized using HRP-conjugated secondary antibodies (Catalog No. AP187P, Millipore) with SuperSignal™ West Femto Maximum Sensitivity Substrate (Catalog No ...
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bioRxiv - Genetics 2020Quote: ... Chromatin-bound antibodies were recovered with 30μl Protein A/G magnetic beads (Millipore 16-663). After reverse crosslinking ...
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bioRxiv - Neuroscience 2021Quote: ... Puromycin-labeled proteins were identified using the mouse monoclonal antibody (1:10,000, Millipore, catalog MABE343). Protein synthesis levels were determined by analyzing total lane density from 10 kDa to 250 kDa ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibody against HCoV-OC43 nucleocapsid (NP) protein was purchased from Millipore (Cat.No. MAB9012). Rabbit polyclonal antibody against human LY6E was obtained from proteintech (Cat.No ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies (listed in Table S4) were pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
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bioRxiv - Microbiology 2021Quote: ... was used for blotting and proteins were stained using the following antibodies: (Sigma-Aldrich AV46863), TMEM41B (Sigma-Aldrich HPA014946) ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein-antibody complex was visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Neuroscience 2019Quote: Antibodies used were: rat anti-myelin basic protein (MBP, 1:200, MAB386, Millipore, MA, USA), rabbit anti-Olig2 (1:200 ...
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bioRxiv - Systems Biology 2019Quote: ... Expression of the CARD-CC proteins was determined with anti-Flag antibody (F-3165, Sigma). Expression of the E-tagged PKC clones with anti-E-tag antibody (ab66152 ...
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bioRxiv - Molecular Biology 2021Quote: ... The following commercially available antibodies were used to detect targeted proteins: anti-SPF45 (Sigma-Aldrich), anti-SF3b155 (MBL Life Science) ...
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bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).
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bioRxiv - Microbiology 2021Quote: ... MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I, Merck Millipore, France).
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bioRxiv - Microbiology 2021Quote: ... The detection of recombinant proteins was analyzed by immunoblot using an anti-myc antibody (Sigma) for SyNOS and a specific OtNOS antibody generated by GenScript Company.
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bioRxiv - Cell Biology 2020Quote: The following proteins were detected by western blotting (antibodies in brackets) CPSF3 (Sigma-Aldrich, HPA04657), IntS11 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... The FLAG M2 antibody used for the detection of MetRSL274G protein was purchase from Sigma. ANL ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using primary antibodies α-FLAG (monoclonal α-FLAG M2, Sigma, 1:1000) or α-Myc (Clone A-14 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were purified from hybridoma culture supernatant using protein G Sepharose 4 Fast Flow (Sigma). Injection of the respective antibody resulted in depletion of >90% of CD4+ or CD8+ T cells for at least 14 days ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies for immunofluorescence included rabbit anti-glial fibrillary acidic protein (GFAP) (EMD Millipore; AB5804), rabbit anti-microtubule associated protein-2 (MAP2 ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Plant Biology 2023Quote: ... was used to detect proteins with horseradish peroxidase-conjugated anti-rabbit secondary antibodies (Sigma, A6154).
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bioRxiv - Pathology 2023Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualized with the FITC- and TRITC-conjugated secondary antibodies (Sigma, F6005, and T5393) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins reacting with primary antibodies were visualized by the Immobilon forte western HRP substrate (Millipore) for detecting peroxidase-conjugated secondary antibodies and analyzed with an ChemiDoc Touch (Bio-rad).