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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Bcl 2 Binding Component 3 BBC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... 1/200 (v/v) each phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich), and 1/100 (v/v ...
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bioRxiv - Immunology 2021Quote: ... mtDNA depletion was induced by 100 μM 2′,3′ dideoxycytidine (ddC, Sigma-Aldrich) in medium supplemented with uridine and sodium pyruvate ...
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bioRxiv - Immunology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Sigma, 28718-90-3, St Louis, MO, USA). The results were recorded using Zeiss LSM780 confocal system (Zeiss ...
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bioRxiv - Biophysics 2020Quote: ... MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, p# M5655, Sigma-Aldrich) was dissolved in Phosphate Buffer Solution (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5mM Na3VO4 and 1% each of phosphatase inhibitor cocktails 2 and 3 (Sigma) and set on ice for 10 minutes prior to sonication ...
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bioRxiv - Cancer Biology 2022Quote: ... the reagent 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) was added to the cells and incubated for 4 hours at 37 °C (5 % CO2) ...
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bioRxiv - Neuroscience 2022Quote: ... buffered with 3 mM N-2-hydroxyethylpiperazine-N’-ethanesulfonic acid (HEPES, Sigma Chemical) and pH adjusted to 7.65 at 15°C ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 3:2:1 in HEK 293T cells (Sigma Aldrich) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The MTT compound (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) was dissolved in RPMI 1640 without phenol red ...
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bioRxiv - Molecular Biology 2022Quote: ... growth medium was supplemented with 100 μM of 2′,3′-dideoxycytidine (Sigma; D5782) or 50 ng/ml of ethidium bromide ...
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bioRxiv - Neuroscience 2022Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2, Phosphatase Inhibitor Cocktail 3, Sigma-Aldrich), and then incubated for 1 hour on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation in a solution of 2/3 dichloromethane (DCM; Sigma-Aldrich), 1/3 MetOH ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium Bromide – MTT (Sigma-Aldrich), MitoTracker probe (Life Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... phosphatase inhibitor Cocktails 2 and 3 were from Sigma Aldrich (St. Louis, MO). Mouse anti-Rac1 antibodies (#ARC03) ...
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bioRxiv - Neuroscience 2023Quote: ... 3-(4,5-dimethylthiazol-2-Yl)-Diphenyltetrazolium Bromide (MTT, CT01-5, Sigma-Aldrich, UK) stock solution was diluted in F12 medium without phenol red (1:5) ...
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bioRxiv - Molecular Biology 2023Quote: ... fed every day and split every 2-3 days using Accutase (A6964, Sigma).
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bioRxiv - Biochemistry 2023Quote: Rosetta-gami 2 competent cells (Novagen / Millipore-Sigma, USA; cat. no. 71350-3)
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bioRxiv - Biochemistry 2023Quote: Rosetta-gami 2 competent cells (Novagen / Millipore-Sigma, USA; cat. no. 71350-3)
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bioRxiv - Neuroscience 2023Quote: ... The animals received unilateral injections totalling 3×2 μg of 6-OHDA (Sigma Chemical CO ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 to 3 million cells were cross-linked using 1% formaldehyde (Sigma, F8775) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... 3-month-old male and female mice were anesthetized with 2% avertin (Sigma) and positioned in a stereotaxic apparatus (David Kopf instruments) ...
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bioRxiv - Neuroscience 2024Quote: ... P8340) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich, P5726 and P0044). Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... Phosphatase inhibitor cocktail 2 (#P5726) and cocktail 3 (P0044) were from Sigma-Aldrich. Glutathione Sepharose 4B (#17-0756-01 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM PMSF and phosphatase inhibitor cocktail 2 and 3 from Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked in 3 % BSA/PBS and incubated with anti-ZEB1 antibodies (Sigma, HPA027524, 1:200 in 3 % BSA/PBS). After washing and incubation with Alexa594-conjugated secondary antibodies (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... A DMEM type media lacking sodium bicarbonate was also generated using individual components (FisherScientific and Sigma) at the concentrations listed in commercial preparations ...
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bioRxiv - Microbiology 2021Quote: All chemicals and M9 minimal media components used in this study were purchased from Sigma-Aldrich. 13C Acetate tracers were purchased from Cambridge Isotope laboratory ...
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bioRxiv - Microbiology 2020Quote: Volatile components analyzed with their respective CAS numbers were purchased from Sigma-Aldrich (Saint Luis, EUA): ethyl acetate (141-78-6) ...
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bioRxiv - Neuroscience 2021Quote: ... Monomer solution components were prepared by dissolving Sodium Acrylate in npH20 (33% w/v, Sigma 408220), Acrylamide in npH20 (50% w/v ...
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bioRxiv - Microbiology 2020Quote: ... the dextran bead component was prepared (Cytodex-1 micro carrier beads, Sigma, St. Louis, MO USA) [22] ...
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bioRxiv - Systems Biology 2022Quote: ... 1,2-13C and 1,6-13C glucose along with unlabeled media components were purchased from Sigma-Aldrich-Merck ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... the individual components of PCANS were sterile filtered using 0.2 μm PVDF syringe filters (EMD Millipore) and combined as described above.
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bioRxiv - Microbiology 2022Quote: ... or a concentration of 50 nM for IMAC-purified DARPins) binding to CagI was performed using a mouse-anti-FLAG M2 monoclonal antibody (dilution 1:5000; Sigma, F1804) as primary and a goat-anti-mouse antibody conjugated to an alkaline phosphatase (dilution 1:10,000 ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody binding was detected using HRP-conjugated anti-rabbit IgG and visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, Burlington, MA) and an ImageQuant LAS4000 digital imaging system (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... Non-specific antibody binding was blocked by applying a mixture of 10% v/v ChemiBLOCKER (Cat# 2170, Millipore, Burlington, MA, U.S.A.) and 5% v/v normal donkey serum in PBS for 12 hours at room temperature with gentle agitation ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were fixed with ice-cold acetone/methanol (1:1 v/v) and a specific antibody binding was blocked with 5% BSA (w/v, Sigma-Aldrich). Organoids were then incubated with primary antibodies against acetylated α-tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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bioRxiv - Developmental Biology 2020Quote: ... in TNT for 2-3 h at room temperature and incubated with an anti-Fluo-POD antibody (1/50, Sigma-Aldrich REF 11 426 346 910) in 2% TNB overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... were reared in 10g/l of glucose/200µM of 3BDO (3-Benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one) (Sigma Aldrich) supplemented in standard fly food ...
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bioRxiv - Biochemistry 2020Quote: ... Day 1 adult animals were mounted on 2% (vol/vol) agar pads and immobilized with 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer.
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bioRxiv - Biochemistry 2020Quote: ... animals were mounted on 2% (vol/vol) agar pads and immobilized in 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Dechorionated live embryos were incubated for 6, 12 and 24 hours in 40μM Nifedipine or 20mM BDM (2, 3-butanedione-2-monoxime) (Sigma-Aldrich) in Danieau’s solution ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]). Samples were centrifuged at 21,000g for 30 min at 25°C to obtain the Triton-insoluble (SDS-soluble ...
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bioRxiv - Neuroscience 2022Quote: ... or DMSO 0.1% as vehicle was evaluated using a MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) assay as described in (Sanz et al. ...
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bioRxiv - Cell Biology 2024Quote: ... purified CAPS-GST protein was mixed with increasing concentrations of microtubules in Ca2+ Binding-Buffer (80 mM PIPEs, 2 mM MgCl2, 50 uM CaCl2, 1 mg/ml BSA, 10 uM leupeptin (Sigma Aldrich L-2884), 1 uM Calpain Inhibitor Peptide (Sigma-Aldrich C-9181;) ...
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bioRxiv - Biochemistry 2019Quote: ... Knock-down of glutamate dehydrogenase 2 (GDH2, encoded by the GLUD2 gene) using siRNAs (#NM_012084: 5’CUAACCUCUUCACGUGUAA’3 and 5’UUACACGUGAAGAGGUUAG‘3, Sigma-Aldrich, Taufkirchen, Germany) or transfection of GLUD2 plasmid/empty vector controls was done using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hrs followed by addition of 3 µM Asunaprevir (Apexio: BMS-650032) and 500 µM 3-Indoleacetic acid (Sigma Aldrich: I2886). Cells were treated with following inhibitors to inhibit indicated kinases ...
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bioRxiv - Biochemistry 2021Quote: ... or 2 μg of mouse IgG control antibody (Sigma) in a rotating platform at 4 °C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprobing was done using primary antibodies (ERK1/2 (Millipore), diluted 1:300 and 4EBP1 (Cell Signaling technologies ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-CMV IE1/2 monoclonal antibody (MAB8131, Millipore), and rabbit anti-ZAP polyclonal antibody (PA5-31650 ...