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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and blocked for at least an hour at 4° in PBST + 3% normal goat serum (Sigma). Tissues were incubated overnight at 4° with gentle agitation with primary antibodies diluted in PBST and washed three times with PBST ...
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bioRxiv - Neuroscience 2023Quote: ... for another 3 days of cultivation before fixing with 4 % paraformaldehyde (PFA, #P6148, Sigma-Aldrich, USA) for 30 min at 37 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Odor stimuli were diluted to 10-3 and 10-4 concentration in hexane (>99.5% purity; Sigma), with hexane used as the solvent control ...
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bioRxiv - Microbiology 2023Quote: ... and 2-heptyl-3-hydroxy-4-quinolone (PQS) were obtained from Sigma Aldrich (St. Louis, MO). Triton X-100 and Tween-20 were used at 0.2% and 2% ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Cells were washed 3 times with PBS and then fixed with 4% paraformaldehyde (PFA, Sigma-Aldrich) for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... followed by tenfold concentration using Amicon Ultra-4 Centrifugal Filter (3 kDa MWCO, Millipore Sigma UFC800396). Custom-designed 4-Plex Luminex kits (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... 3 colonies of each construct were selected and incubated in 4 mL LB medium (Sigma #L3022) with 1:1000 carbenicillin overnight in a shaker at 37°C and 250 RPM ...
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bioRxiv - Synthetic Biology 2023Quote: ... Assays were stopped by filtration at 14,000g and 4 °C with 3 kDa spin filters (Millipore). After filtration ...
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bioRxiv - Microbiology 2023Quote: L-Sulforaphane (4-methyl sulfinyl butyl isothiocyanate, 4MSOB-ITC; ≥ 95 %, CAS 142825-10-3, Sigma-Aldrich) or allyl-ITC (allyl isothiocyanate ...
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bioRxiv - Biochemistry 2024Quote: ... Tspan12 siRNA duplexes (#1: 5’-GCUUAUCUUUGCCUUCUCCTT-3’ and 5’-GGAGAAGGCAAAGAUAAGCTT-3’; #2: 5’-AUGAGGGACUACCUAAAUATT-3’ and 5’-UAUUUAGGUAGUCCCUCAUTT-3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was carried out at 4°C overnight with the following antibodies: mouse anti-Calbindin-D-28K (Sigma, C9848, 1:1000), rabbit anti-vGlut2 (Synaptic Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC; Sigma Aldrich #39391), and 3NPH solutions were mixed ...
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bioRxiv - Neuroscience 2021Quote: ... followed one hour later by 3 ng/mL IL1α (Sigma #I3901), 400 ng/mL C1q (MyBioSource #MBS143105) ...
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bioRxiv - Microbiology 2020Quote: ... blocked for one hour with 3% normal goat serum (Sigma-Aldrich) diluted in PBS-0.1% Tween20 (PBST) ...
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bioRxiv - Biochemistry 2020Quote: ... and 4×10^-4 1-Thioglycerol (Sigma Aldrich), and incubated for 72 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; 1 μM; #H7904 Sigma) or vehicle was added to the cell culture medium to activate Cre-LoxP recombination ...
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bioRxiv - Molecular Biology 2020Quote: ... After permeabilization with 0.5% Triton X-100 in PBS for 10 min at 4°C and blocking with 3% bovine serum albumin (BSA) (Wako) in PBS with 1% DAPI (Sigma-Aldrich) at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellets were resuspended at an OD600 of 1 in 5 ml of agroinfiltration buffer (10 mM MgCl2 and 250 μM of 3’,5’-Dimethoxy-4’-hydroxyacetophenone (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated for 24 hours with either 1 mM 3-Hydroxy-1,2-dimethyl-4(1H)-pyridone (Deferiprone/DFP, Sigma-Aldrich, 379409), or incubated in Earl’s balanced salt solution (EBSS ...
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bioRxiv - Cell Biology 2022Quote: ... thereafter differentiations were kept in RB- without small molecule inhibitors until day 3 (d3) before the WNT inhibitor IWR-1 (4 μM, Sigma, #I0161) was added for 48 h ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma, F3165). Each reaction was incubated with Protein-G agarose beads (50 μL ...
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bioRxiv - Genetics 2023Quote: ... at 4°C for 1 h followed by overnight incubation with 3 μL of the following antibodies: H3K4me2 (Millipore 07-030), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Cancer Biology 2023Quote: ... IFI44L (#1: TRCN0000167757; #3: TRCN0000242541) or ISG15 (#4: TRCN0000007423)) in the presence of X-tremeGENE HP DNA Transfection Reagent (Sigma Aldrich). Five shRNAs per gene were tested for knockdown efficiency ...
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bioRxiv - Pathology 2022Quote: ... in PBS, 2x) and soaked in 12.5% (2h; 4°C) and 25% (7 days, 4°C) sucrose (Sigma). Kidneys were embedded in OCT (Tissue-Tek® ...
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bioRxiv - Microbiology 2022Quote: ... the samples were permeabilized with TritonX-100 at 0.1% in D-PBS with subsequent blocking with 3% Bovine Serum Albumin (Sigma-Aldrich) in D-PBS ...
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bioRxiv - Genomics 2020Quote: BJ cells (Fig. 3) and A549 cells (Fig. S10) were treated with 5 μg/mL of Actinomycin D (Sigma, A9415), which inhibits transcription by halting elongation of the transcribed RNA (42 ...
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bioRxiv - Biochemistry 2020Quote: ... Bound protein was eluted seven times with 0.5 column volumes of buffer A with 3 mM D-Desthiobiotin (Sigma-Aldrich), 0.026% DDM and 0.1 mM AEBSF protease inhibitor ...
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bioRxiv - Biochemistry 2022Quote: ... The column was washed with 30 column volumes (CV) of wash buffer before bound proteins were eluted with wash buffer supplemented with 3 mM D-desthiobiotin (Sigma). The protein was transferred into wash buffer without D-desthiobiotin and incubated overnight at 4°C with 1:100 (w/w ...
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bioRxiv - Genetics 2022Quote: ... Slides were then rinsed in washing buffer five times for 3 min each and nuclei were stained with 4lr,6-diamidino-2-phenylindole (DAPI, cat # D-1388, Sigma) at a concentration of 1 μg/ml for 10 min at RT ...
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bioRxiv - Immunology 2020Quote: ... 8-20 weeks after reconstitution chimeric mice were injected intraperitoneally for 3 d with 2 mg/day of tamoxifen (Sigma) re-suspended at 20 mg/ml in corn oil (Sigma ...
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bioRxiv - Immunology 2021Quote: ... were pelleted at 3.000g for 3 minutes and induced by resuspension in YNB-CAA + 2% D-(+)-Galactose (Sigma G0625-500G) + phosphate buffer + P/S induction medium at a final OD600 of 0.5 ...
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bioRxiv - Pathology 2023Quote: Mouse epidydimal adipose tissue was harvested and digested in Hanks’ Balanced Salt Solution (HBSS, no calcium and magnesium) containing fetal bovine serum (FBS,3%) and collagenase D (2.5 mg/ml, Sigma-Aldrich) for 30 minutes at 37°C under agitation ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g L−1 D-(+)-maltose (Sigma-Aldrich), 1 g L−1 D-(+)- fructose (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g L−1 D-(+)-cellobiose (Sigma-Aldrich), 1 g L−1 D-(+)-maltose (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g L−1 D-(+)- fructose (Sigma-Aldrich) and 0.5 g L−1 L-cysteine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... cells were treated with fresh media containing 50 μM D-biotin (Sigma B-4501). After 24 hours of labeling ...
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bioRxiv - Neuroscience 2020Quote: ... cultures were treated with 1mM Cytosine b-D-arabinofuranoside hydrochloride (Ara-C) (Sigma Aldrich) to inhibit glial cell proliferation ...
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bioRxiv - Biophysics 2021Quote: ... D-(+)-Glucose (D-Glu; Sigma-Aldrich, 20 mM) or L-(–)-Glucose (L-Glu ...
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bioRxiv - Systems Biology 2021Quote: ... plus D-glucose (EMD) and D-galactose (Sigma). Cultures were grown in a humidified incubator (Infors Multitron ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... D-mannitol and D-trehalose dihydrate (Sigma-Aldrich) at a concentration between 50 and 500 mg/mL were prepared by simple mixing (Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... Dead cells and red blood cells were excluded using 1 μg/ml 7-amino actinomycin D (7AAD; Sigma-Aldrich), Fixable Viability Dye eFluor® 780 (Invitrogen ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were plated onto selective Lysogeny broth (LB)-agar plates supplemented with 1 mM Isopropyl b-D-1-thiogalactopyranoside (IPTG) (no. I6758; Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal ...
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bioRxiv - Cell Biology 2020Quote: ... Following staining with secondary antibodies and 0.1 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma, D-9542) diluted in 5% NGS-PBS ...
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bioRxiv - Bioengineering 2021Quote: ... and 4-arm 10,000 kDa PEGDA crosslinker (Creative PEGWorks) were all reconstituted in 0.1% photoinitiatior (Irgacure D-2959, Sigma Aldrich) at 1% w/v ...
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bioRxiv - Plant Biology 2019Quote: ... For Pchlide and heme assays seedlings were then grown for 4 d dark at 22 °C with or without 0.1 or 0.2 mM aminolevulinic acid (ALA; Sigma Aldrich) with 5mM MES ...
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bioRxiv - Immunology 2020Quote: ... Controls included cells cultured at 4°C and 37°C in the presence of 10μM cytochalasin D (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: Indicated cells were cultured in phenol red free culture medium for 48 hours before supernatant was collected and incubated with 0.2 mM of the chromogenic plasmin substrate D-Val-Leu-Lys 4-nitroanilide dihydrochloride (Sigma). After 30 minutes absorbance was recorded at 405 nm on a 96-well microplate reader (Infinite F50).