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Citations for Millipore Sigma :
501 - 550 of 6391 citations for 15 PGDH HPGD Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2020Quote: ... 4°C through a 15 mL 50 kDa MW cutoff concentrator (Amicon Ultra −15 Centrifugal Filters, Millipore Sigma) or individual elution fractions from the heparin column were stored ...
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bioRxiv - Molecular Biology 2019Quote: ... Isolated SCGs were placed in Leibovitz’s L-15 media (Leibovitz’s L-15 with 0.4% D(+)-glucose) and incubated in collagenase (1 mg/mL, Sigma) and trypsin (0.25% ...
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bioRxiv - Neuroscience 2021Quote: The rotenone stock solution (15 mg/ml) was prepared by dissolving 15 mg Rotenone (Sigma Aldrich, Lot#MKBz2534V) in 1 ml DMSO (dimethyl sulfoxide) ...
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Early life lipid overload in Native American myopathy is phenocopied by stac3 knock out in zebrafishbioRxiv - Genetics 2023Quote: ... stac3+/+ and stac3+/-: n=15 and stac3-/-: n=15 larvae were fixed in 4% paraformaldehyde (PFA, Sigma-Aldrich) either for 1 hour at RT or overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: The cAMP synthesis of heterologously expressed sAC in HEK293 lysates was induced by applying di-sodium-ATP (Sigma Aldrich) and MgCl2 or MnCl2 in isotonic HEPES buffer ...
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells transfected and grown overnight prior to incubation with 5μg/ml cycloheximide (CHX; Sigma-Aldrich, St. Louis, MO) in the presence and absence of 15μM CQ (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...
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bioRxiv - Biophysics 2020Quote: ... 15 mM d-ribose (R9629, Sigma Aldrich), 1% FBS and 1 M NaOH to achieve a final pH of 7.0-7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... and anti-β-actin (AC-15; Sigma).
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bioRxiv - Cell Biology 2020Quote: ... 15% (v/v) β-mercaptoethanol (Sigma-Aldrich), pH 6.8 ...
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bioRxiv - Immunology 2021Quote: ... anti-β actin (clone AC-15; Sigma), anti-Caspase-1 (3866 ...
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bioRxiv - Genetics 2021Quote: ... Anti-β-actin antibody (AC-15, Sigma), were used for Western blot ...
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bioRxiv - Developmental Biology 2021Quote: ... + 15% fetal bovine serum (Sigma-aldrich F2442), 1:100 pen-strep (Gibco 15140-122) ...
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bioRxiv - Developmental Biology 2021Quote: ... in 1mL L-15 medium (Sigma-Aldrich) with 0.26 U Librase (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... 15 μM MG-132 (Sigma-Aldrich, C2211) and 0.5 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies against b-actin (AC-15, Sigma) and Na+/K+ATPase a (H300 ...
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bioRxiv - Biophysics 2020Quote: ... 15 mM d-ribose (R9629, Sigma Aldrich), 1% FBS and 1 M NaOH to achieve a final pH of 7.0-7.4 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 15% pluronic gel (P2443-250G, Sigma, USA) was prepared in PBS at 4°C and mixed with MK-1775 to a final concentration of 500 nm (HY-10993 ...
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bioRxiv - Molecular Biology 2019Quote: ... also 15 µl aprotinin (Sigma-Aldrich #A6279), 0.5 µl leupeptin (Sigma-Aldrich #L2884 ...
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bioRxiv - Genomics 2020Quote: ... and 15 μg/ml PMA (Sigma-Aldrich), with the pH adjusted to 7.323,25,34 ...
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bioRxiv - Genomics 2021Quote: ... 15% fetal bovine serum (FBS) (Sigma, F7524), 0.1 mM -mercaptoethanol (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... 15 g mL-1 agar (Millipore, 12177) in H2O ...
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bioRxiv - Bioengineering 2021Quote: ... and 15 mM triethanolamine (TEA; Sigma-Aldrich) dissolved in phosphate buffered saline (PBS) ...
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bioRxiv - Biophysics 2021Quote: ... 9-15 U/mL pyruvate dehydrogenase (Sigma), 13.5-21 U/mL lactate dehydrogenase (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... alkylated with 15 mM iodoacetamide (Sigma-Aldrich) and precipitated with trichloroacetic acid (TCA ...