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Citations for Millipore Sigma :
5401 - 5450 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... resuspended in serum-purified human polyclonal IgG or IgA (I2511-10MG and I4036-1MG, Sigma Aldrich), and incubated at 4°C for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Inserts were previously coated with 50 µl of human collagen IV at 30 µg/ml (Millipore) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and the oocytes were matured in vivo with 5 U of human chorionic gonadotropin (hCG; Sigma) before mating with B6D2F1/J males (F1 hybrid of a cross between C57BL6/J and DBA2/J ...
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bioRxiv - Bioengineering 2023Quote: ... laevis females were injected with 400 µL of human chorionic gonadotropin (2000 IU/mL, Sigma-Aldrich) and incubated for more than 16 h to induce ovulation in an incubator at 16°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were re-plated onto collagen (2.4ug/ml) (Type IV from human placenta Sigma Cat# C5533) coated plates in E8 with 10uM ROCK inhibitor (ROCKi ...
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bioRxiv - Cell Biology 2023Quote: Human VSMCs were isolated as previously described90 and were cultured in Dulbecco’s modified Eagle medium (Sigma) supplemented with 20% fetal calf serum ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... Anti-human-DLL4 (Rabbit) and anti-LAM (rabbit) were from Sigma Aldrich (St. Louis, MO, USA). Anti β-ACTIN (rabbit) ...
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bioRxiv - Cell Biology 2023Quote: ... with subsequent maintenance of adherent cells in RPMI 1640 medium supplemented with 10% human serum (Sigma) and MCSF (10 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... flasks and plates were pre-coated with 10 µg/mL of human plasma fibronectin (Sigma-Aldrich) for 1h at room temperature before washing three times with water ...
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bioRxiv - Molecular Biology 2023Quote: ... Human primary fibroblasts were cultured in DMEM high-glucose with 1mM sodium-pyruvate (Sigma-Aldrich, # P5280) supplemented with 20% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the sequence of the shRNA targeting 3UTR of human NRF2 gene was obtained from Sigma Aldrich (TRCN0000007555 ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used for im-munofluorescence in human cells: Mouse anti-G-Tubulin (Sigma T6557 ...
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bioRxiv - Cell Biology 2024Quote: PC3 human prostate cancer cells (ATCC, CRL-1435) were cultured in HAM F12 medium (Sigma‒Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were grown on vitronectin coated plates containing PluriSTEM® Human ES/iPS Cell Medium (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Pathology 2024Quote: ... the cells were cultured on culture dishes coated with 0.05mg/ml human placenta derived COLIV (Sigma) in Bronchial epithelial cell growth medium with added supplements according to the manufacturer’s recommendation (BEBM-Lonza ...
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bioRxiv - Cancer Biology 2024Quote: Tumors were harvested as mice reached human endpoint and fixed in neutral buffered formalin (Sigma-Aldrich) for 24-48h ...
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bioRxiv - Cell Biology 2024Quote: MISSION shRNAs against human KIF1A (SHCLNG-NM_004321) and KIF5A (SHCLNG-NM_004984) were obtained from Sigma-Aldrich. Scramble vector was the MISSION pLKO.1-puro non-Mammalian shRNA Control vector ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/mL dsDNA or 10 μg/mL recombinant human insulin (Sigma Aldrich, St. Louis, MO). After five washes in PBS with 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2024Quote: Glycated Aβ (gAβ) was prepared by incubating 10 mg/ml of human Aβ1-42 (Sigma-Aldrich) with or without 0.5 mM methylglyoxal (MGO ...
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bioRxiv - Microbiology 2024Quote: Primary human foreskin fibroblasts (HFFs) (ATCC) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) with 4.5 g/L glucose ...
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bioRxiv - Immunology 2024Quote: ... Plates were then washed and incubated with HRP-conjugated goat anti-human IgG antibody (Sigma-Aldrich) (diluted 1:2500 in PBS-T ...
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bioRxiv - Molecular Biology 2024Quote: 50,000 human iPS-CM were seeded/well in Lab-Tek 4-well chamber slides (Sigma-Aldrich) and cultured for five days according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Human embryonic kidney 293 (HEK293) cells were cultured in RPMI-1640 medium (Sigma, St. Louis, MO) with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... HGF concentration in the CM was determined using the human HGF ELISA Kit (Sigma-Aldrich #RAB0212) following manufacturer protocol.
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bioRxiv - Evolutionary Biology 2019Quote: ... Plants were analyzed for production of proteins from transgenes via western blot hybridizations (explained above) with anti-FLAG M2 monoclonal primary antibody (Sigma-Aldrich, order number: F3165, diluted 1:1000) and anti-mouse-HRP secondary antibody (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated overnight with primary antibodies at the following concentrations: chicken anti-Microtubule Associated Protein 2 (MAP2) polyclonal antibody (Sigma-Aldrich, EMD Millipore, Darmstadt, Germany, AB5543, 1:1000) and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated overnight at 4 ° C using antibodies specific for the N-terminal amino acids 66 – 81 of the amyloid precursor protein (APP) (Millipore, Burlington MA, clone 22C11 at 1:80K), GFAP (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then precipitated and resuspended in digestion buffer (100 mM Tris pH 8.5, 1% sodium deoxycholate [Sigma-Aldrich], 5 mM TCEP, and 30 mM CAA). Trypsin and Lys-C proteases were added to a 1:25 and 1:100 ratio (weight/weight) ...
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bioRxiv - Biochemistry 2023Quote: ... was used to plot elution profiles of the protein standard mix Supelco® 69385 Protein Standard Mix 15-600 kDa (Sigma-Aldrich/Merck, Darmstadt, Germany), and the analyzed protein samples.
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bioRxiv - Bioengineering 2023Quote: ... loaded to a polyacrylamide gel (30 μg of total protein/lane) and after gel electrophoresis and protein transfer to polyvinylidene difluoride (PVDF) membranes (Millipore, cat. no. IPVH00010, Burlington, MA). The membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... 167 mM NaCl) and precleared for 2 h with 250 μL of previously blocked 50% protein-A and protein-G beads (Sigma P-7786 and P-3296 respectively). Blocking was achieved by incubating the beads with 0.5 mg/ml of Ultrapure BSA and 0.2 mg/mL of salmon sperm DNA for 4 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... For p65 IP the lysates were then incubated with 2 μg of antibody CST ref #6956 or CST ref# 8242) at 4 °C for 2 hrs before adding 20 μL of slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose) for 20 min ...
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bioRxiv - Biophysics 2020Quote: ... then immersed in a solution of Human Plasma Fibronectin (10 ug/mL in PBS; #FC010, EMD Millipore), and washed thoroughly in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were probed with antibody specific for human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), mouse P2X4 (Cat ...
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bioRxiv - Immunology 2021Quote: ... Plasma samples from four Danish non-pregnant women without malaria exposure and purified human IgG (Sigma-Aldrich) were included as negative controls.
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... hiPSCs were plated at a density of 4-5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12 well plate in GK15 medium (GMEM [Life Technologies] with 15% KSR ...
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bioRxiv - Neuroscience 2021Quote: ... A 100x high purity N2 solution was made with 10 mg/ml human holo-transferrin (Sigma T4132), 0.5mg/ml human recombinant insulin solution (Sigma I9278) ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... The DNA-binding domain of human Timeless (DBD; residues 816-954) was cloned into pRSF-Duet1 (Novagen) with an N-terminal His14-SUMO tag and expressed in E ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured in Click’s medium supplemented with 10% heat-inactivated human serum (Sigma-Aldrich, H4522), 1% L-glutamine and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... and primary human monocytes were treated with 100 ng/ml LPS (O26:B6) (Sigma Aldrich; Cat.# L2654) for 3-4 hrs ...
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bioRxiv - Cell Biology 2019Quote: ... 4H1 mouse monoclonal antibody targeting amino acids 35 to 44 of human ORF1p40,67,68 (Millipore, cat. number MABC1152) was used at 1:15000 dilution ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... mice were further injected with 5 IU (0.1 mL/animal) human chorionic gonadotropin (hCG) (Sigma-Aldrich, USA). Twenty hours after PMSG injection ...
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bioRxiv - Microbiology 2019Quote: ... Parasites were grown in human O+ erythrocytes at 4% hematocrit in complete RPMI1640-HEPES media (Sigma-Aldrich) supplemented with 0.3% L-glutamine ...
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bioRxiv - Microbiology 2019Quote: Human foreskin fibroblasts (HFFs) were cultured in minimum essential medium Eagle (MEM; Sigma-Aldrich, St. Louis, Missouri) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: Human HepG2 cell line was grown in Dulbecco’s Modified Eagle’s medium – high glucose (Sigma-Aldrich®, D6429) supplemented with 10% fetal bovine serum (PAN Biotech ...
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bioRxiv - Bioengineering 2019Quote: ... and fibrinogen (FBG; from human plasma, 20 mM sodium citrate-HCl pH 7.4, Lot #3169957, Millipore Sigma). FAM-proteins were prepared with 10 mg of protein reconstituted in 900 μL of 0.1 M PBS and 8-fold molar excess of FAM NHS ester solubilized in 100 μL DMSO ...
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bioRxiv - Immunology 2019Quote: ... human liver tissue was minced with scissors and digested in 5mg/ml pronase (Sigma-Aldrich, P5147-5G), 2.93mg/ml collagenase B (Roche ...
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bioRxiv - Immunology 2019Quote: ... using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel/Immunology Multiplex Assay (EMD Millipore #HCYTOMAG-60K): GM-CSF ...