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Citations for Millipore Sigma :
5351 - 5400 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Eluted proteins were buffer exchanged and concentrated with PBS using Amicon tubes (Millipore). The proteins were further purified by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2021Quote: ... All proteins were concentrated by ultrafiltration with Amicon Ultra (10 kDa, Millipore, Germany) and protein purity was confirmed by SDS– PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated to > 150 μM using centrifugal filters (Amicon Ultra; Merck Millipore), aliquoted and snap-frozen and stored at −80 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein transfer was confirmed with Ponceau S stain (0.1% w/v Sigma-Aldrich). Membranes were blocked for 5 minutes using undiluted EveryBlot Blocking Buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a PVDF membrane (Immobilon-FL; Merck Millipore, Billerica, MA). Immunoblotting was performed with primary antibodies against Camk2a phosphorylated at pT286 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined using the BCA method (QuantiPro assay kit; Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... and total protein was detected with Amido Black (Sigma-Aldrich, St. Louis, MO) as previously described 51 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were prepared in RIPA buffer (containing protease (1:100; Sigma, P8340) and phosphatase inhibitors (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto 0.2 μm polyvinylidene fluoride (PVDF) membranes (Millipore, Hertfordshire, UK) for 1 hour at 100V ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was transferred onto PVDF paper (Millipore Sigma, Cat # IVPH00010, Billerica, MA, USA) for 90 mins at 0.5 amps with a Power Pac HC power supply (Bio Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Dried protein hydrolysates were re-dissolved in 16µL HPLC grade pyridine (Sigma 270407) prior to derivatization with 20µL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert–Butyldimethylchlorosilane (Sigma 375934 ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was then transferred onto a polyvinylidene difluoride membrane (Millipore, Toronto, Canada) and probed using antibodies for PI4KIIIβ (BD Biosciences 611817 ...
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bioRxiv - Biochemistry 2021Quote: ... Viral supernatants were filtered (0.45 μm low protein binding filter unit (EMD Millipore)) and concentrated 10-20-fold (Amicon Ultra-15 concentrator with a 100,000-dalton molecular mass cutoff) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then resolved by SDS-PAGE and immunoblotted using GST (Sigma Aldrich), 6His (Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from protein backbone by PNGase F incubation (Sigma Aldrich) for 12 h at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore Corp., Billerica, MA) and blocked-in tris-buffered saline-Tween 20 (TBST ...
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bioRxiv - Cell Biology 2021Quote: Thermophilic Odinarchaeota proteins were expressed in Rosetta (DE3) pLysS Escherichia coli cells (Novagen). Cultures were grown at 37°C to an OD600 of 0.3 then cooled to 25°C and further grown to an OD600 of 0.6 and induced overnight with 0.33 mM IPTG ...
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bioRxiv - Plant Biology 2021Quote: Total protein was extracted with 2X Laemmli buffer (Sigma-Aldrich, cat no. 33401) containing 5% β-mercaptoethanol by boiling at 95°C for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected by Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, MA, USA) and a LAS-3000 mini chemiluminescence imaging system (Fujifilm ...
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bioRxiv - Plant Biology 2021Quote: ... FLAG-tagged proteins were detected with Monoclonal ANTI-FLAG M2-Peroxidase (Sigma #A8592) at a dilution of 1:5,000 ...
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bioRxiv - Microbiology 2020Quote: ... The collected HemATN protein samples were concentrated using an Amicon ultrafiltration cell (Millipore) and dialyzed into dialysis buffer (50 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration was determined using a Direct Detect® Infrared Spectrometer (Millipore Sigma).
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bioRxiv - Plant Biology 2021Quote: ... Secreted MIK2ECD-6xHIS and BAK1ECD-6xHIS proteins were purified using Ni-NTA (Novagen) and size-exclusion chromatography (Hiload 200 ...
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bioRxiv - Neuroscience 2020Quote: ... while rat anti-glial fibrillary acidic protein (1:2500, AB5804, Millipore, Lot # TA265137) was used for the evaluation of astrocytes ...
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bioRxiv - Neuroscience 2020Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Immunology 2021Quote: ... Bound protein was eluted in presence of 1M methyl α-D-mannopyranoside (Sigma). The protein was dialyzed against PBS and subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 GL (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Eluted protein was desalted using PD-10 desalting columns (Sigma-Merck, U.S.A/Germany). Concentration of the desalted protein was estimated using Bradford’s method and the protein was stored at -80 °C until needed.
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bioRxiv - Plant Biology 2021Quote: ... The purified proteins were concentrated using an Amicon Ultra-4 10 K (Millipore).
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bioRxiv - Plant Biology 2021Quote: ... and protein expression was induced by addition of doxycycline 1μg.mL-1 (PHR1145, Sigma). 6 hours after doxycycline addition ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration was determined using a Direct Detect® Infrared Spectrometer (Millipore Sigma).
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bioRxiv - Microbiology 2021Quote: ... followed by transfer of the proteins onto Immobilon FL PVDF membrane (Millipore, Germany). Antibodies against the T3SS components EspA (1:500) ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... the protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). A90C α-synuclein was labelled with 1.5-fold molar excess Alexa Fluor 488 C5 maleimide (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was concentrated using Amicon Ultra-15 centrifugal filter units (Millipore, Germany) to around 8 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were resolved by SDS-PAGE and transferred onto PVDF membranes (Millipore). Membranes were blocked with 2% BSA in TBS tween20 0.1% and incubated in the presence of the primary and then secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were reduced with 10 mM Tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich) for 1 h at room temperature followed by alkylation of cysteine residue side chain thiol groups with iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
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bioRxiv - Biophysics 2021Quote: ... The protein fractions were pooled and concentrated using 3kDa cutoff ultracentrifugal device (Millipore). The protein was analyzed on 12% SDS-PAGE and mass spectrometry ...
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bioRxiv - Biophysics 2020Quote: ... The protein was then concentrated via centrifugal filters (MWCO 10 kDa; Millipore Sigma) and the buffer was exchanged into final buffer by PD-10 desalting column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred to a methanol activated polyvinylidene difluoride (PVDF) membrane (Millipore) in Bis-Tris or Tris-Glycine transfer buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... and loading control protein □-Tubulin (Sigma Aldrich ref: ref T9026, diluted 1/20,000). The secondary antibodies used were anti-mouse IgG ...
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bioRxiv - Genomics 2021Quote: ... Dialyzed Tn5 protein was concentrated by using ultracel 30-K column (Millipore, UFC903024) and the quantity of Tn5 was measured by Bradford assay and visualized on NuPAGE Novex 4-12% Bis-Tris gel (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% glycerol with 5000x diluted SYPRO® Orange protein gel stain (Sigma-Aldrich) in total reaction volume 25 μl ...
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bioRxiv - Cell Biology 2021Quote: ... Equal total proteins loads were confirmed by Ponceau red (0.1% Ponceau, Sigma-Aldrich), 5% acetic acid (v/v)] staining of the membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge and flash frozen in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... The protein complexes were eluted with wash buffer containing 10 mM desthiobiotin (Sigma) and treated with TEV protease at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... individual plasmids were transformed into BL21 Rosetta (DE3) protein expression cells (EMD Millipore).
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bioRxiv - Biochemistry 2022Quote: ... the protein was first digested at 16°C with an immobilized pepsin (Sigma) cartridge (2 mm x 2 cm ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinyl difluoride membranes (Millipore) according to the standard protocols ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated by SDS-PAGE and transferred overnight to nitrocellulose membranes (Millipore). Membranes were blocked with 5% bovine serum albumin in tris-buffered saline with 0.05% Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... bead-bounded proteins were reduced with 100 mM Dithiothreitol (DTT) (Sigma-Aldrich, 43815) and alkylated using 100 mM iodoacetamide (Applichem ...