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Citations for Millipore Sigma :
5351 - 5400 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... proteins were transferred to a PVDF membrane (Immobilon®-P, Millipore) with a semi-dry transfer system (Trans-Blot® SD ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were mixed with 2x Laemlli sample buffer (S3401, Sigma) and heated at 95°C for 10 minutes prior to SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates were collected using Radio-Immunoprecipitation Assay (RIPA) buffer (Sigma), supplemented with protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... After protein quantification by the bicinchoninic acid colorimetric assay system (Sigma), 1mg protein samples were used for HDAC3 immunoprecipitation using four microliters of HDAC3 antibodies (Cell Signalling ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: ... and the resolved proteins were then transferred to PVDF membrane (Millipore). The membrane was blocked using 5% skimmed milk and then immunoblotted with primary antibodies at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with trypsin (TPCK Treated; Sigma; Cat. # 4352157). iTRAQ labeling of the peptides in each sample was performed using the iTRAQ Reagents Multiplex kit (AB Sciex) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was treated with 10 U PreScission protease (Sigma) at 4°C for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were concentrated using a 10 K Amicon (Merck-Millipore), flash-frozen in liquid nitrogen and stored at −80°C until use.
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were recovered by binding to protein A/G agarose (Millipore), and the eluted DNA fragments were used directly for qPCR or subjected to high-throughput sequencing (ChIP-Seq ...
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bioRxiv - Cell Biology 2022Quote: ... and phosSTOP if phosphorylated proteins were being investigated (Sigma-Aldrich, 04906845001)] ...
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bioRxiv - Cell Biology 2022Quote: ... After protein transfer membranes were incubated in Ponceau solution (Sigma-Aldrich) to confirm even transfer ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined by Bradford assay kit (Sigma-Aldrich, B6916). Lysates were mixed with 5 × sample buffer (250 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... stained with 1:200 mouse anti-ubiquitinylated proteins (Sigma #04-263) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were electro-transferred to a polyvinylidene fluoride membrane (Millipore) using Turbo-Blot Turbo transfer system (settings ...
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bioRxiv - Microbiology 2022Quote: ... affinity purified IcsAp protein was mixed with 0.1 mM DSP (Sigma) in PBS and incubated at room temperature for 30 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... resuspended and lysed in 200 μL Bugbuster protein extraction reagent (Millipore). Following another centrifugation ...
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bioRxiv - Plant Biology 2022Quote: ... the proteins were transferred on a PVDF membrane (Immobilon-P, Millipore) using a wet blotting system ...
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bioRxiv - Plant Biology 2022Quote: ... samples were incubated with 25 μl of protein A beads (Millipore) for 2 h in cold room (100 μL were collected as input) ...
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bioRxiv - Neuroscience 2022Quote: ... Resulting protein lysates were precipitated with trichloroacetic acid (TCA) (Sigma, T0699). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-phosphoepitope SR proteins clone1H4 (Millipore, cat#MABE50, 1:2,000 dilution) were used as primary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... as measured by a BCA protein assay kit (Sigma-Aldrich, USA).
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bioRxiv - Plant Biology 2022Quote: ... Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore, USA). The membrane was incubated with rabbit anti-C ...
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bioRxiv - Molecular Biology 2023Quote: ... resolved proteins were transferred onto Immobilon-P PVDF membranes (Millipore Sigma) using a Bio-Rad Trans-blot® semi-dry transfer cell ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were then reduced with 10 mM dithiothreitol (D9779, Sigma), followed by alkylation with 30 mM Iodoacetamide (I1149 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was then transferred onto Infrared PVDF membranes (Millipore, Burlington, MA) and membranes were blocked in Immobilon Blok Solution (Millipore ...
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bioRxiv - Physiology 2022Quote: ... Proteins were visualized using a chemiluminescence (ECL) kit (Millipore, MA, USA). Target protein expression was normalized to β-actin (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... Target protein expression was normalized to β-actin (Sigma-Aldrich, #2066) or vinculin (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was pre-cleared with Protein G agarose beads (Sigma) and anti-rabbit IgG (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... were firstly incubated with the Protein-G agarose (Millipore, 16-266) for 2 hrs ...
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bioRxiv - Bioengineering 2022Quote: ... anti-Fatty acid binding protein 1 (FABP1) (1:50, Sigma-Aldrich) and anti-cytokeratin 20 (CK20 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein was concentrated using a 10-kDa Amicon filter device (Millipore) and aliquoted into 1 mg aliquots for further use.
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bioRxiv - Physiology 2022Quote: ... to standardize protein loading in Laemmli 2X Concentrate sample buffer (Sigma) and deionized water for gel electrophoresis (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... Total protein was quantified with Bradford reagent (SIGMA, cat. no. B6916) and 25 μg of extract were suspended 1:6 (v/v ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore; #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-P, Millipore, IPVH00010) at 100 mA overnight ...
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bioRxiv - Bioengineering 2023Quote: ... protein was extracted by using RIPA lysis buffer (20-188, Millipore) supplemented with cOmplete Mini protease and phosphatase inhibitors tablets (Roche) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... After transferring the proteins to PVDF membranes (Millipore, Etobicoke, ON, Canada) as described ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated with Amicon Ultra-15 centrifugal filters (Millipore) and buffer exchanged to storage buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto PVDF membranes (Millipore, Billerica, MA, USA), the membranes were quenched and proteins were detected using specific antibodies ...
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bioRxiv - Genomics 2024Quote: ... The protein concentration was measured with the Bradford Assay (Sigma-Aldrich). Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated using Amicon Ultra centrifugal filter units (Merck Millipore). Protein was then aliquoted ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins in the gel bands were reduced with dithiothreitol (DTT) (Sigma) and alkylated by iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was concentrated using Amicon Ultra Centrifugal Filter units (Millipore) and further purified by size exclusion chromatography using a Superdex 75 prepgrade column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was blotted off with filter paper (Sigma–Aldrich, WHA1001110), and immediately after blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins then transferred to 0.45 µM Immobilon-FL PVDF (Millipore Sigma) after methanol activation ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to a PVDF membrane (Millipore, catalog #IPVH00010) at 100V for 30 min in transfer buffer (20% methanol ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...