Labshake search
Citations for Millipore Sigma :
5351 - 5400 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Embryos were harvested at E11.5 and N=3 WT and mutant forelimbs (pooled left and right) were dissociated into single cell suspensions using collagenase (Sigma C9697; 50mg/mL) at 37°C for 15 minutes followed by gentle pipette mixing ...
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bioRxiv - Microbiology 2024Quote: ... and influenza A/California/7/2009 H1N1pdm09 virus were amplified on MDCK α2-6 in MEM supplemented with 1% HEPES and 1 μg/ml trypsin treated with N-tosyl-L-phenylalanine chloromethyl ketone (Sigma, Oakville, ON, Canada). Viral titers were determined by plaque assays ...
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bioRxiv - Immunology 2020Quote: ... then 25 ug total protein was separated by 10% SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk and probed with the indicated primary antibodies following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were eluted twice with 2 ug/uL V5 peptide in water (Sigma-Aldrich, St. Louis, MO) at 37°C for 15 minutes with shaking at 1000rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 ug protein/sample was diluted in 1X LDS Sample Buffer and 50 mM DTT (Sigma-Aldrich), then incubated for 10 minutes at 70°C ...
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bioRxiv - Physiology 2023Quote: ... We injected frogs with 0.5 ug ACTH per g body weight (Sigma-Aldrich®, product no. A7075) diluted in 0.9% saline and a vehicle injection of only 0.9% saline with 6 days between injections ...
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bioRxiv - Immunology 2022Quote: ... aegypti SGE was diluted to a concentration of 2 ug/ul in carbonate-bicarbonate buffer (Millipore-Sigma) and used to coat 96-well Immulon 4x HB plates (Nunc ...
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bioRxiv - Immunology 2022Quote: ... aegypti SGE was diluted to a concentration of 2 ug/ul in carbonate-bicarbonate buffer (Millipore-Sigma) and used to coat 96-well Immulon 4x HB plates (Nunc ...
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bioRxiv - Molecular Biology 2023Quote: ... and each peptide fraction was then cleaned up using 2 ug capacity C18 ZipTips (Millipore, Billerica, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mg of protein from each sample were incubated with 3 ug of HOXA9 (Millipore # 07-178) or Rabbit IgG Isotype control (Invitrogen # 02-6102 ...
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bioRxiv - Molecular Biology 2021Quote: ... transformed with HsaBR (or its mutant HsaBR R340E) was inoculated in 25 ml of LB media in presence of 100 μg/ml ampicillin (Millipore, 171254-25GM) and grown overnight at 37°C with mild shaking of 225 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... transformed with YenB (or its mutant YenB S31A/Y100F) was inoculated in 25 ml of LB media in presence of 100 μg/ml ampicillin (Millipore, 171254-25GM) and grown overnight at 37 °C with mild shaking of 225 rpm ...
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bioRxiv - Synthetic Biology 2020Quote: ... pastoris cells were grown in liquid YPD or solid YPD-agar and selected for with the appropriate antibiotics: 100 µg/mL Zeocin® or 300 µg/mL BlasticidinS-HCl (Sigma Aldrich). Transformants of auxotrophic strains were selected on Complete Supplemented Medium (CSM ...
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bioRxiv - Neuroscience 2019Quote: ... and transcardially perfused with 100 ml of ice cold 0.1M PBS followed by 200 ml of 4% paraformaldehyde (pH 7.4; Sigma-Aldrich, St. Louis, MO). Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S ...
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bioRxiv - Systems Biology 2021Quote: ... mice were euthanised by intraperitoneal injection of 200 µl of 200 mg/ml pentobarbital (Dolethal®) supplemented with 100 U/ml heparin (cat.# H3393, Sigma Aldrich), and adult worms recovered by portal perfusion (the portal vein is sectioned followed by intracardiac perfusion with phenol-red-free DMEM ...
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bioRxiv - Cancer Biology 2022Quote: ... tumors were cut into small pieces and digested for 1.5 hours at 37 °C in 5 ml of DMEM/F12 containing 2 mg/ml of collagenase type I (WAKO, no. 037-17603) and 100 U/ml of hyaluronidase (Sigma, no. H3506). Following digestion ...
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bioRxiv - Cancer Biology 2022Quote: ... Livers were weighted and 50% of the tissue was sliced in small pieces and incubated 30 minutes at 37°C in 10 ml of digestion medium (RPMI GlutaMAX medium [Gibco] containing 200U/ml of collagenase type IV [Sigma-Aldrich] and 100 U/ml of DNAse I [Sigma-Aldrich]). Remaining undigested fragments were syringed with an 18G needle and filtered through a 70µm cell strainer to obtain a single cell suspension ...
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bioRxiv - Developmental Biology 2020Quote: ... KSOM without BSA was supplemented with 1 mg/mL PVA from a stock of 100 mg/mL PVA in water (Millipore-Sigma, P8136). Where indicated heparin (Millipore-Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were eluted with 4 mL of the same buffer but containing 100 μL (5 mg mL−1) Flag peptide (Sigma-Aldrich F4799). The eluate was spin concentrated (Sigma-Aldrich CLS431485-251A ...
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bioRxiv - Cell Biology 2022Quote: ... 10−3μl and 10−4μL lentivirus mixed in 0.5 mL media and 0.5 μL of 100 mg/mL Polybrene (Millipore Sigma Cat. TR-1003-G) in duplicate wells ...
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bioRxiv - Immunology 2022Quote: ... BLaER1 cells were trans-differentiated for 5-6 day in medium containing 10 ng/mL IL-3, 10 ng/mL M-CSF (MPI of Biochemistry, Munich) and 100 nM β-estradiol (Sigma-Aldrich) at a concentration of 7 × 104 cells/well in 96 well plates ...
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bioRxiv - Microbiology 2024Quote: ... the extracellular bacteria were removed in all samples by adding 250μl appropriate cell culture medium supplemented with 100 μg/ml gentamicin and 5 μg/ml lysostaphin (Sigma-Aldrich Cat# L7386) (gentamicin-lysostaphin protection assay ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL PDL and 100 to 200 µg/mL laminin (Merck/Sigma-Aldrich #L2020). For biochemical experiments ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 h in serum- free RPMI-1640 (with 10,000 U/mL Penicillin, 10 mg/mL Streptomycin, 1:100) (Sigma-Aldrich, Sydney, Australia) at a density of 0.2 × 106 cells/cm2 ...
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bioRxiv - Neuroscience 2019Quote: ... solubilized in 100% sunflower seed oil by sonication (100 mg/kg body weight, 20 mg/ml stock solution, #T5648; Millipore Sigma, St. Louis, MO) for five consecutive days12 ...
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bioRxiv - Neuroscience 2021Quote: ... solubilized in 100% sunflower seed oil by sonication (100 mg/kg body weight, 20 mg/mL stock solution, #T5648; Millipore Sigma, St. Louis, MO) for five consecutive days (Chucair-Elliott et al. ...
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bioRxiv - Immunology 2021Quote: ... which were diluted 1:100 in triplicate into 5 mL fresh CHG media containing either 100 μM of the corresponding substrate (either LCA [Sigma] or 3-oxoLCA [Steraloids]). Cultures were grown for 48 hours at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... solubilized in 100% sunflower seed oil by sonication (100 mg/kg body weight, 20 mg/mL stock solution, #T5648; Millipore Sigma, St. Louis, MO) for five consecutive days ...
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bioRxiv - Neuroscience 2023Quote: ... solubilized in 100% sunflower seed oil by sonication (100□mg/kg body weight, 20□mg/ml stock solution, #T5648; Millipore Sigma, St. Louis, MO) for five consecutive days54 ...
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bioRxiv - Cell Biology 2020Quote: ... 150,000 cells were stained with 10 μM 4’-(trimethylammonio)-diphenylhexatriene (TMA-DPH) or 2 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine; both obtained from Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... All chemicals used for sample expansion (glutaraldehyde 50% in water, sodium acrylate, N, N’-methylenbisacrylamide, acrylamide, Proteinase K) were purchased from Sigma Aldrich and used as received.
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bioRxiv - Cell Biology 2022Quote: Late L4–young adult mlt-4:RFP mutants (GUN405) were mutagenized for 4 hr at 22 °C in 0.5 mM N-nitroso-N-ethylurea (ENU, Sigma Aldrich N3385). Worms were washed in M9 buffer (22 mM KH2HPO4 ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.1% β-mercaptoethanol (Cat N° 31350010)) supplemented with 5 μM Y-27632 (Merck Millipore, Burlington, MA, USA; Cat N° 688001), 0.2 μM LDN-193189 (Stemgent ...
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bioRxiv - Biochemistry 2021Quote: ... and pcDNA5/FRT/TO Myc SARS CoV2 N and N 2A was transfected into HEK293T cells using GeneJuice (Novagen, Darmstadt, Germany) following the manufacturer’s protocol (12 μg of DNA/sample in total) ...
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bioRxiv - Microbiology 2021Quote: ... Protein of interest was dialyzed using Slide-A-Lyzer Dialysis Cassette 10,000 MWCO (ThermoScientific, Cat. N° 66810) and concentrated using Amicon Ultra Centrifugal filters 10,000 MWCO (Millipore, Cat. N° UFC901024). Purity was verified by Coomassie staining after SDS-PAGE and concentration was measured by Bradford assay.
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bioRxiv - Biophysics 2020Quote: ... 1.4 g of the catalyst N,N,N0,N0- tetramethylethylenediamine was added before 1.4 g of ammonium persulfate (both reagents purchased from Sigma Adrich, USA), which initialized polymerization ...
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bioRxiv - Cell Biology 2021Quote: ... the carboxylic acid groups on the Dynabeads® were activated by a 30-minute incubation with N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDC, cat. num. 39391, Sigma) and N-Hydroxysuccinimide (NHS ...
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bioRxiv - Biochemistry 2020Quote: Mevaldehyde was prepared using a mevaldic acid precursor (the hemi-[N,N’-dibenzylethylenediammonium] salt of mevaldic acid) purchased from Sigma Aldrich[18] ...
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bioRxiv - Microbiology 2022Quote: ... n-Alkane identification and quantification was performed using an external standard mixture of C21-C40 n-alkanes (PN 04071, Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... The first and second extractions were combined for each sample and 250 µL of this combined solution was added to a 300 µL glass insert in a fresh glass septum-topped vial containing and the SCFAs were derivatized using 25 µL N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA; Sigma Aldrich) at 60°C for 30 minutes.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... reacted with a mixture of 50 μL N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide (MTBSTFA; Sigma-Aldrich Co. LLC., St Louis, MO, U.S.A.) and 50 μL acetonitrile at 60°C for an hour.
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bioRxiv - Immunology 2020Quote: Nexterion-E microscope slides (Schott, Jena, Germany) were amino-functionalized with a 2% w/v linear copolymer of N,N′-dimethylacrylamide (Sigma-Aldrich) and aminoethyl methacrylate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were then derivatized for gas chromatography analysis using 50 µL solution of 1:1 pyridine: N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (chemicals obtained from Sigma Aldrich) and incubated at 60 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: For in vivo studies, AZD5153 (Chemitek, #CT-A5153), AZD5363 (Chemitek, #CT-A5363) and NHWD870 were first dissolved in N,N-dimethylacetamide (Sigma, #270555) and then mixed with 0.5% methylcellulose (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... All chemicals used for sample expansion (glutaraldehyde 50% in water, sodium acrylate, N,N’-methylenbisacrylamide, acrylamide) were purchased from Sigma Aldrich and used as received ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were derivatized for GC-MS analysis by adding 10 μL of pyridine and 50 μL of N-Methyl-N-(trimethylsilyl)trifluoroacetamide (Sigma-Aldrich) to the residue ...
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bioRxiv - Systems Biology 2023Quote: ... followed by an incubation at 40°C for 1 h and then adding 70 μl of MSTFA (N methyl-N-trimethylsilyltrifluoroacetamide; Sigma-Aldrich) followed by another incubation at 40°C for 2 h ...
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bioRxiv - Bioengineering 2023Quote: ... Then the samples were immersed in a pre-gel solution containing 30 wt % hydrogel monomers (N,N′-dimethylacrylamide; DMAA, Sigma-Aldrich) and 1 wt % Irgacure 2959 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: AD-293 cells were seeded and transfected the next day with either pcDNA5/FRT/TO-N-FLAG-hBirA*-GEMC1/MCIDAS or pcDNA5/FRT/TO-N-FLAG-hBirA* using PEI (Sigma-Aldrich) ±50 μM biotin (IBA GmbH ...
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bioRxiv - Neuroscience 2023Quote: ... male (N = 2) and female (N = 2) naïve mice were infused with Fluorogold (Sigma, St. Louis, MO; 4% in sterile saline) in the DMS (0.2 µL) ...