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Citations for Millipore Sigma :
5251 - 5300 of 10000+ citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-α-tubulin (T6199; IF: 1/4000) and pAb anti-Flag-tag (F7425; IF: 1/500, WB: 1/1000) were from Sigma-Aldrich, and a pAb anti-GFP (632592 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GSTπ at 1:3000 (MBL, #311), rabbit anti-IBA1 at 1:500 (Wako, #01919741) and rabbit anti-GFAP at 1:1000 (Millipore, #OBT0030). Sections were washed three times with PBS and stained using biotinylated anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were incubated overnight with primary antibodies (Anti-DNALI1: 1: 100, Abcam, Cat No: ab87075; Anti-PACRG: 1: 200, generated by our laboratory; Anti-α-tubulin, 1:200, Sigma, Cat No ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated with the rabbit anti-NeuN (1:800, Beyotime Biotechnology) or monoclonal anti-Dicer 1(1:300, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Strains were seeded in lysogeny broth (LB, 10 g L-1 NaCl, 10 g L-1 tryptone, 5 g L-1 yeast extract, Sigma-Aldrich) at 30°C with shaking overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... anti- Spz (rat, 1:50, requested from Satoshi Goto), Anti-Elav (rabbit, 1:50, DSHB) and Caspase 3 (rabbit, 1:500, Sigma Aldrich). Samples were washed four times for 10 minutes each in PBST and incubated at room temperature in secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected into 293T cells at a 1:1:1:1.6:4.6 ratio using 293 Free transfection reagent (EMD Millipore Cat #72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were co-transfected into 293T cells at a 1:1:1:1.63:4.63 ratio using 293 Free transfection reagent (EMD Millipore Cat# 72181) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were then derivatized for gas chromatography analysis using 50 µL solution of 1:1 pyridine: N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (chemicals obtained from Sigma Aldrich) and incubated at 60 °C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were dissolved in 1 ml acetone/water (1:1, Carl Roth, Karlsruhe, Germany) and 0.1 % trifluoroacetic acid (Sigma Aldrich, Dreieich, Germany). 170-200 µL DHB matrix solution was applied to the sections using a pneumatic sprayer system with a nitrogen pressure of 0.7-0.75 bar ...
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bioRxiv - Cell Biology 2020Quote: ... then plated onto LB plates containing the appropriate antibiotic (100 μg ml−1 ampicillin, 50 μg ml−1 kanamycin or 25 μg ml−1 chloramphenicol, Sigma Aldrich). Plasmid DNA was extracted from bacteria using GenElute™ Plasmid Miniprep Kit (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-βactin (AC-15, 1:20000) and mouse anti-FLAG (F-3165, 1:7000; IF 1:300) from Sigma-Aldrich; rabbit anti-TOP1 (A302-589A,1:5000) ...
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bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... and incubated with goat anti-mouse antibody conjugated to alkaline phosphatase (Sigma-Aldrich; A3688-1 MI; 1:10,000 1% BSA/PBS) for 2 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; GAPDH, 1:10,000 dilution, Millipore; PAFAH/PLA2G7 ...
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bioRxiv - Neuroscience 2022Quote: Small and large intestines were dissected from E14 embryos and digested with 1 mg/ml DNAse 1 (AppliChem A3778) and 1 mg/ml collagenase A (Merck Millipore 10103586001) in DMEM-F12 at 37 °C while shaking ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... either tarsi from all six legs or the proboscis of individual animals was stimulated three times at five seconds intervals with a drop of an appetitive solution (1 M sucrose, Fisher Chemicals, Waltham, Massachusetts, USA, CAS # 57-50-1; or 1 M D-glucose, Sigma-Aldrich, Saint Louis ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2, 1:100, Millipore; mouse anti-GRIN2BB ...
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bioRxiv - Immunology 2023Quote: ... The fresh tissue was dissected followed by digestion for 2 hours at 37°C in 1 mg ml−1 Collagenase 1 (Sigma-Aldrich). Debris was removed and cells isolated by sequentially passing through 100 ...
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bioRxiv - Immunology 2023Quote: ... Staining was conducted in PBS / 1 % BSA (CHO cells and huPBMCs) or PBS / 1 % BSA / 1 % normal mouse serum (bovine cells) (Sigma-Aldrich) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were ground in an ice-cold buffer (50 mmol.L-1 TRIS, 5 mmol.L-1 EDTA, 2 mmol.L-1 DTT, protease inhibitor cocktail (Sigma, St. Louis, MO, USA)) ...
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bioRxiv - Neuroscience 2022Quote: The samples were placed ventral side up in ECi-cleared phytagel blocks (1 x 1 x 1 cm, Sigma-Aldrich, P8169). These were placed in a sample holder ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% (v/v) Penicillin-Streptomycin (Pen-Strep, 100 U mL−1 and 100 μg mL−1 respectively, Sigma-Aldrich, #P4333). 3T3-L1 cells were cultured routinely in DMEM supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... stained with primary antibodies overnight (1:500 mouse anti-Tubulin β3, BioLegend; 1:500 rabbit anti-Sox11, abcam; 1:500 rabbit anti-Sox9, Sigma-Aldrich; 1:250 rabbit anti-Stat3 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 16uL of 10000 units mL-1 (approximately 6 mg mL-1) penicillin G and 10 mg ml-1 streptomycin solution (Sigma-Aldrich) was added to each tray to inhibit the growth of pathogens (Gangur & Marshall 2020) ...
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bioRxiv - Immunology 2023Quote: ... 18 h after cell transfer recipient mice were immunized into the right hind paw with 50 μl of a 1:1 emulsion of 1 mg/ml MOG35-55 peptide (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (incomplete Freund’s adjuvant (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... 100 mM KCl; 1% NP-40; 1 mg/ml sodium heparin; 1 mM DTT; 100 μg/ml cycloheximide [#C4859-1ML, Millipore Sigma] ...
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bioRxiv - Cell Biology 2022Quote: ... Cell layers were incubated with primary antibodies against fibrillin-1 (described previously, 1:1000) and fibronectin (clone FN-15, 1:1000, Millipore-Sigma) diluted in blocking buffer for 2 h at RT [31] ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% SDS (10 mM Tris-HCl, pH 8.0; 1% SDS; 1 mM Na-Orthovanadate; 1x Protease Inhibitor Cocktail (Sigma-Aldrich; P8340)) and incubated for 10 min at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with CF568/AttoN647-conjugated goat anti-mouse or anti-rabbit (1:100–1:400, Thermo Fischer Scientific) and Alexa 488/568-conjugated phalloidin (1:200, Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 90%, 100%), incubated in acetone/Spurr resin (1:1, 30 minutes, 1:2, 30 minutes) and in Spurr resin (Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich) and 5 ng/ml FGF2 (Peprotech) ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-monoclonal PSD95 (1:3000, MAB1598) and synaptophysin (1:10000, MAB329-C) (Burlington, MA), mouse monoclonal anti-actin (1:10000, A5441) from Millipore Sigma. Primary antibody incubations were conducted overnight at 4 °C followed by incubation with the appropriate HRP conjugated secondary antibody for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were restimulated after 72 h of culture with PMA (50 ng ml-1) and ionomycin (1 µg ml-1, both from Sigma-Aldrich) in the presence of brefeldin A (5 µg ml-1 ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
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bioRxiv - Plant Biology 2024Quote: ... Transformants were selected with appropriate antibiotic(s) (hygromycin B, 20 µg mL-1; paromomycin, 10 µg mL-1; spectinomycin, 100 µg mL-1 Sigma-Millipore) for a week before screening them for fluorescence in 96 well plates ...
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bioRxiv - Microbiology 2024Quote: ... Subsequently these cysts were washed three times with PBS and incubated with secondary anti-rabbit IgG conjugated with Alexa flour 488 (1:300) and Calcofluor White 1:20 (CFW, 1 mg/ml, Sigma Aldrich) for 30 min at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Rabbit α-mouse Calmodulin (5μg mL-1, CellSignaling), rabbit α-mouse β-actin (1μg mL-1, CellSignaling) and mouse α-mouse GAPDH (1μg mL-1, Merck/Millipore), goat-α-mouse IRDye 680RD ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used in this study were as follows: mouse anti-Actub (1:1,000 or 1:2,000, Sigma, 6-11B-1), guinea pig anti-Ick (1:100 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (EMD Millipore Corp.; Cas: 367-93-1). Cells were then incubated for 18 to 20 hours at 20°C at 200 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with DMEM:F12 (1:1)+10% FBS containing Polybrene (Sigma, USA) at a final concentration of 4 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... 1:5 or 1:10 (v/v) ratio with sinapinic acid matrix (Sigma; 10 mg/mL in water/acetonitrile/trifluoroacetic acid ...
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bioRxiv - Developmental Biology 2021Quote: ... The slides were stained with 1% cresyl violet and 1% eosin Y (Sigma) in 75% ethanol (Clément-Ziza et al. ...
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bioRxiv - Genetics 2021Quote: ... 0.0025% bromophenol blue) supplemented with protease inhibitor (1% Millipore Sigma P8340, 1% PMSF), incubated at 100°C for 3 minutes ...
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bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... 1% v/v antibiotics and 1% v/v carboxymethyl cellulose (CMC) (Sigma-Aldrich), and incubated at 37°C and 5% CO2 for 4 days ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... rabbit anti-Aggrecan (1:1000 for E13.5, 1:1500 for E16.5, Millipore AB1031), rabbit anti-Lef1 (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... brains were processed for CtB (1:300,000) and cFos (Millipore #PC38, 1:10,000) expression using previously published procedures (Glover et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% non-essential amino acids and 1% l-glutamine (Sigma-Aldrich, Dorset, UK).