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Citations for Millipore Sigma :
5151 - 5200 of 10000+ citations for Urotensin 2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 2 mM vanadyl ribonucleoside complexes (Sigma-Aldrich), 0.2 mg/mL RNase-free bovine serum albumin (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 mM ascorbate (A4034, Sigma-Aldrich), Complex IV activity was measured ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 2-mercaptoethanol (Millipore-Sigma, 63689) directly in wells and RNA was extracted and purified using RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Systems Biology 2024Quote: ... 2 mM L-glutamine GlutaMAX (Sigma-Aldrich) and 1x penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cumate (2–50 µg/mL, Sigma #268402) was added at log phase (A600 ...
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bioRxiv - Neuroscience 2024Quote: ... 5-bromo-2’-deoxyuridine (BrdU; Sigma-Aldrich) was intraperitoneally injected into the mice for the last 5 days of ZSS treatment ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.05 mM L-ascorbate-2-phosphate (Sigma) and 10 mM β-glycerphosphate (AppliChem GmbH ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM forskolin (Sigma catalog #344270) with media replenished every 2 days.
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bioRxiv - Immunology 2024Quote: ... or PHA (2 μg/ml) (Sigma-Aldrich). Results were expressed as cell division index which is the ratio of %CFSElow splenocytes (proliferating splenocytes ...
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bioRxiv - Cancer Biology 2024Quote: ... L-Glutamine 2 mM (Sigma-Aldrich; #G7513) and Plasmocin 25 µg/mL (InvivoGen ...
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bioRxiv - Neuroscience 2024Quote: ... and 2 % Penicillin-Streptomycin (Sigma-Aldrich, P4333). Rat astrocytes (Gibco™ ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM sodium pyruvate (Sigma #S8636-100ML), 100 μM 2-mercaptoethanol (Sigma #63689–25 ML-F) ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 2-mercaptoethanol (Millipore-Sigma, 63689) directly in wells and RNA was extracted and purified using RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... the ORF of CLEL6 lacking the predicted signal peptide was amplified by PCR from a previous construct and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). Correct orientation and translational fusion with the C–terminal His-tag was verified by sequencing ...
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bioRxiv - Immunology 2021Quote: ... 1 mM penicillin-streptomycin and 55 μM β-mercaptoethanol) containing 10 nM either OVA257-264 (H2N-SIINFEKL-OH) or YopE69-77 peptide and brefeldin A (BFA, Sigma, 5 μg/ml) for 4.5 h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... The beads were washed tree times with TBS solution and FLAG-HDA6 was eluted with 200 ng/µl of Flag-Peptide (Sigma-Aldrich, Cat No F3290) by incubation the resin with synthetic peptide rotating for 30 min at 4 °C.
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bioRxiv - Plant Biology 2020Quote: ... ORFs of PSK1 and RGF1 lacking the predicted signal peptides were amplified by PCR from cDNA and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). C-terminal His tags were added by including 6 His codons in the reverse PCR primers ...
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bioRxiv - Microbiology 2019Quote: ... and added to wells in a sterile 96-well microtiter plate containing two-fold dilutions of each peptide with 0.01% v/v acetic acid (Sigma Aldrich, St Louis, MO, USA) and 0.2% w/v bovine serum albumin (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were subsequently washed another three times with 1 ml FLAG wash buffer II and eluted in 100 μl FLAG elution buffer (FLAG wash buffer II with 0.5 mg/ml Flag peptides (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066, RRID: AB_476693, immunogen: synthetic peptide corresponding to the C-terminal actin fragment SGPSIVHRKCF, Sigma-Aldrich, St. Louis, MO, USA), and rabbit polyclonal anti-AA-NAT 1:15,000 diluted in PBST (AB3314 ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were immunized on day 0 by subcutaneous injection in the flank region with a total of 200 μl of the antigen PLP139-151 peptide (200 μg/mouse) emulsified in Complete Freund’s Adjuvant CFA (Sigma Chemicals, St. Louis, MO, USA) supplemented with 4 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (400 μg ...
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bioRxiv - Bioengineering 2024Quote: ... The molecular weight of the synthesized peptides was confirmed using MALDI-TOF Mass (MALDI-TOFMS AXMA ASSURANCE™ [Shimadzu]) with α-cyano-4-hydroxycinnamic acid matrix™ (Sigma-Aldrich). Synthesized peptide sequences for ELISA are shown in Table S4.
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bioRxiv - Biophysics 2021Quote: ... 2 μL of 100 mM 5,5′-dithiobis-(2-nitrobenzoic acid) (Sigma Aldrich, dissolved in 50 mM NaH2PO4, pH 7.5) was immediately added and fully mixed by pipetting before incubating at room temperature for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... These were digested with 2 mg/ml collagenase IV and 2 mg/ml hyaluronidase (Sigma-Aldrich, St Louis, USA) at 37 °C for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl, 2 mM EGTA, 2 mM MgCl2, 0.5% NP-40, 1mM DTT, 1U/ml Benzonase from Novagen/EMD Millipore ...
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bioRxiv - Immunology 2021Quote: ... BMDMs were pretreated for 30 min with hexokinase inhibitor 2-deoxy-D-glucose (2-DG; 10 mM, Sigma-Aldrich), actin polymerization inhibitor cytochalasin D (10 μM ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Peptides were separated using a 250 min gradient ranging from 2 % to 90 % mobile phase B (mobile phase A: 2% acetonitrile [Scharlab S.L.], 0.1 % formic acid [Sigma-Aldrich] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2-Methoxy-3-methyl-1,4-BQ was synthesized as follows: 2 mmol 2-Methoxy-3-methyl-1,4-hydroquinone (Sigma Aldrich) and 2 mmol NaIO4 were mixed in a 50 ml round-bottom flask and 10 ml CH2Cl2 and 5 ml of water were then added ...
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bioRxiv - Cancer Biology 2019Quote: ... Reagents used including 5-chloro-2′-deoxyuridine (CldU) and 5-Iodo-2′-deoxyuridine (IdU) were obtained from Sigma-Aldrich. Concentration and duration of treatment are indicated in the corresponding figures and sections.
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bioRxiv - Immunology 2019Quote: Cell viability was monitored by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT; Sigma, USA) assay as previously described [52] ...
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bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with 2 μg/mL 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Missouri, USA) for 10 minutes as the final step in all flow cytometry assays to distinguish membrane integrity and disruption ...
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bioRxiv - Microbiology 2021Quote: ... pellets were resuspended in 2 ml 10 mM Tris HCl (pH 7.4) containing 2 mg ml−1 pronase (Sigma). Tubes were incubated for 3 h at 60 °C to digest proteins ...
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bioRxiv - Bioengineering 2020Quote: ... yeast extract 5 g/L, 2.7 g/L Na2HPO4, 1.5 g/L citric acid, 2% glucose, 2% cellulase from Trichoderma reesei [Sigma-Aldrich]) in shaking conditions at 250 rpm/min and at 30°C for 3 days ...
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bioRxiv - Cancer Biology 2020Quote: Both pBabe-SAOS 2 and hTERT-SAOS 2 (107 cells) were cross-linked with 1% paraformaldehyde (Sigma Aldrich, # P6148) in culture medium for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Triton-X buffer was supplemented further with 2 μM olaparib (Selleckchem) and 2 μM PARG inhibitor PDD00017273 (Sigma Aldrich)
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bioRxiv - Cell Biology 2021Quote: β-cell like organoids (day 31) were fixed in a freshly prepared mixture of 2 % formaldehyde and 2 % glutaraldehyde (Sigma) in 0.1 M phosphate buffer (18.2% 0.1 M KH2PO4 ...
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bioRxiv - Neuroscience 2019Quote: ... They were then transferred to carbogenated ACSF without sucrose (NaCl, 125; KCL, 3.5; NaH2PO4, 1.25, NaHCO3, 26; CaCl2, 2; MgCl2, 2; glucose, 20; from Sigma-Aldrich) and were used for experiments after at least one hour ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated at room temperature in carbogenated aCSF (125 NaCl, 25 NaHCO3, 2.5 KCl, 1.25 NaH2PO4, 2 CaCl2, 2 MgCl2 and 25 glucose, all from Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... cells growing in either 6-well plates or 10-cm dish in log phase were rinsed with PBS twice and lysed on ice in 100-500 ul 2x Sample buffer (80 mM Tris pH6.8, 2% SDS, 10% Glycerol, 0.0006% Bromophenol blue) containing 280 mM 2-Mercaptoethanol (Sigma #M7522), 1x Aprotinin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 126 NaCl, 2.5 KCl, 1.25 NaH2PO4, 2 MgCl2, 2 CaCl2, 26 NaHCO3, and 10 glucose, 311 mOsm, Sigma-Aldrich) at 34°C for at least one hour before recording ...
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bioRxiv - Biophysics 2019Quote: ... 200 μl of RPMI medium containing a distinct concentration of PMA (0.2, 2, 20, 200 nM) or lipopolysaccharide from Pseudomonas Aeruginosa (LPS) (0.2, 2, 20, 200 μg/ml) (Sigma-Aldrich) were added to a well in a blinded fashion and phase contrast as well as DAPI channels were recorded every two minutes for 160 minutes to image the cell movement.
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bioRxiv - Immunology 2021Quote: Murine NK cells were incubated for 2 h in the presence of GABA (2 or 10 μM, Sigma-Aldrich), supernatants from unchallenged mBMDCs or supernatants from mBMDCs challenged with T ...
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bioRxiv - Microbiology 2019Quote: ... MRS was supplemented with 2% glucose (glc) (MRS-G) or 2% fructose (frc) (MRS-F) (Sigma-Aldrich, Espoo Finland) or less (1.75% ...
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bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
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bioRxiv - Biophysics 2021Quote: ... extraction solution #1 was replaced by extraction solution #2 (2% Triton X-100, 1% CHAPS hydrate; Sigma Aldrich, Germany) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... the animals were infused with 2 ml/kg of 2% Evans blue dye in saline (Sigma Aldrich, MO, USA) through the tail vein ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with 50 µL of a 2 mg/mL solution of (3-(4,5-dimethyl-thiazole-2-yl)-2,5-biphenyl tetrazolium (MTT, Sigma-Aldrich) in PBS for 4 h and then exposed to 100 µL dimethyl sulfoxide (DMSO ...