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Citations for Millipore Sigma :
5101 - 5150 of 10000+ citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... after relaxation in 7% MgClphy2 (Sigma-Aldrich), using NucleoSpin Tissue DNA purification kit (MACHERY-NAGEL) ...
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bioRxiv - Developmental Biology 2024Quote: ... 1:500 Cytokeratin-7 (Millipore Sigma MABT1490), and 1:200 E-cadherin (BD Biosciences 610181) ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked for a minimum of 1 hour at room temperature in 5% (w/v) non-fat milk or 5% (w/v) BSA (A4503-50G; Sigma Aldrich) in TBS-T (1x TBS and 0.1% Tween-20 ...
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bioRxiv - Biochemistry 2020Quote: ... The instrument response function (IRF) of the system was measured using a dilute aqueous solution (3% w/w) of LUDOX AM colloidal silica (Sigma-Aldrich). The full-width half maxima (FWHM ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked at room temperature with either 5% (w/v) non-fat milk or 3% (w/v) BSA (Sigma- Aldrich) in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were re-suspended in fresh medium and viability and cell numbers were monitored with vital dye Trypan Blue 0.2% w/v (Trypan Blue 0.4% w/v, Sigma-Aldrich, St. Louis). The starting number of cells for the titration experiments on cells cultured under adherent conditions was set at 2 × 104 cells in 100μl of culture medium ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated in TBST-5 % w/v milk (TBST supplemented with 5 % (w/v) skimmed milk powder) with anti-rabbit-HRP secondary antibody (A0545, Sigma Aldrich) (1:5,000 ...
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bioRxiv - Plant Biology 2019Quote: ... 2004) containing 1% (w/v) sucrose and solidified with 1% (w/v) EDTA-washed Agar Type M (Sigma-Aldrich, Steinheim, Germany), followed by stratification in the dark at 4 °C for 2 d ...
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bioRxiv - Cell Biology 2021Quote: ... we made 200 μl of gelation solution by mixing 188 μl of monomer stock solution (1 × PBS, 2 M NaCl, 8.625% (w/w) sodium acrylate (SA)(Sigma Aldrich 408220), 2.5% (w/w ...
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bioRxiv - Cancer Biology 2020Quote: One-dimensional aligned microfibers were created from a 21% (w/w) poly lactic-co-glycolic acid, PLGA (LACTEL) solution dissolved in hexafluoroisopropanol (HFIP, Krackeler) with 1% NaCl (Sigma Aldrich) and stirred overnight ...
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bioRxiv - Immunology 2020Quote: ... The butyrate-enriched diet consisted of control feed (V1534, ssniff) supplemented with 10% (w/w) of the butyrate-polymer tributyrin (Sigma Aldrich), as this ensures the release of butyrate in the colon after metabolization by the intestinal microbiota instead of its absorption in the proximal small intestine 46,47 ...
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bioRxiv - Plant Biology 2022Quote: ... seedlings were grown vertically on half-strength MS plates supplemented with 1% (w/v) sucrose and 0.5% (w/v) Phytagel (Sigma-Aldrich, P8169). Five-day-old seedlings were moved to liquid half-strength MS medium containing 2 μM synthetic RALF1 peptide (Sangon Biotech Co ...
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bioRxiv - Neuroscience 2024Quote: ... Demyelination was induced in 8-week-old male mice via a 0.2% w/w cuprizone diet (Sigma-Aldrich, cat# C9012-25G). The diet was freshly prepared by mixing cuprizone with standard powder food and water ...
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bioRxiv - Neuroscience 2024Quote: ... or 1 year old were fed with standard rodent chow mixed with 0.2% cuprizone (w/w; bis-cyclohexanoneoxaldihydrazone; Sigma Cat# C9012) to induce demyelination ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 hour at room temperature in 5% (w/v) non-fat milk (Marvel) or 5% (w/v) BSA (Sigma-Aldrich) depending on the antibody specifications ...
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bioRxiv - Neuroscience 2023Quote: ... Baseline images were acquired on day 0 and then the mice were placed on a 0.2% (w/w) cuprizone (Sigma Aldrich #C9012) diet mixed with ground chow ...
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bioRxiv - Biochemistry 2023Quote: ... No. H7379) and human hemoglobin A0 (HbA0) (with 5% (w/w) methemoglobin) (Cat. No. H0267) (all protein samples from Sigma, USA). Tetrahydrofuran (THF ...
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bioRxiv - Cell Biology 2024Quote: ... each chamber contains 2 mL of respiration buffer (90 mM KCl [P4504, Sigma], 50 mM MOPS [M1254, Sigma], 1 mM EGTA [E4378, Sigma], and 0.1% w/w BSA [A6003, Sigma], pH 7.2) with 5 mM KH2PO4 [P5379 ...
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bioRxiv - Microbiology 2022Quote: ... 5 mL of whole human blood was layered onto 5 mL of Histopaque-1077 Hybri-MaxTM (Sigma Life Science, H8889-100 mL) in a column ...
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bioRxiv - Cell Biology 2023Quote: ... 20 ml or 0,2 ml of pre-warmed DMEM supplemented with heat-inactivated FBS and 100 μg/ml gentamicin (Sigma-Aldrich, G1264-1G) was added to kill extracellular bacteria after which the infection was allowed to proceed for an additional 2 h ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and 0.5 ml of penicillin‒streptomycin (10 000 units penicillin, 10 mg streptomycin/ml, P4333 – 100 ml, Sigma‒Aldrich, St. Louis, USA) were added ...
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bioRxiv - Immunology 2022Quote: ... Cells were cultivated in DMEM medium and stimulated with 10 ng/mL LPS from Salmonella enterica diluted from 1 mg/mL stock (L5886 Sigma-Aldrich, Taufkirchen, Germany) with culture medium ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with Bodipy 409/505 1 ng/mL for 1 h and then incubated with DAPI (Sigma-Aldrich, 1 μg/ml) for 5 min at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... Culture medium for AML193 cells was supplemented with 0.005 mg/ml insulin and 5 ng/ml GM-CSF (Sigma-Aldrich, St. Louis, MO). Cells were cultured at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Immunology 2021Quote: Lung-derived lymphocytes were stimulated in vitro with PMA (50 ng/mL) and ionomycin (1 μg/mL) (both Sigma-Aldrich, St. Louis, MO) for four hours at 37°C in the presence of both Brefeldin A (GolgiPlug ...
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bioRxiv - Immunology 2022Quote: ... dispersed cells were cultured for 4 h in RPMI/10% fetal calf serum (FCS) with 1.5 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (all from Sigma-Aldrich, St Louis, Mo) with Golgi-Stop (BD Bioscience ...
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bioRxiv - Neuroscience 2022Quote: ... cells were first stimulated with phorbol myristate acetate (PMA, 50ng/mL, Fisher BioReagents, cat. #50-058-20001) and ionomycin (750 ng/mL, Millipore Sigma, cat #AAJ62448MCR) in the presence of GolgiStop (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells (2×105 cells/mL) were incubated in the culture medium containing 200 ng/mL of phorbol myristate acetate (PMA) (Sigma-Aldrich; Merck KGaA) for 24 h to induce differentiation into macrophage cells ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... Colonies were picked and grown in 5 ml liquid SD-L-W medium and the prey vectors were isolated using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, USA) after cell wall disruption ...
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bioRxiv - Immunology 2019Quote: ... erythrocytes were depleted by sequentially layering 10 ml PBS followed by 8 ml of 6% w/v dextran (high molecular weight, Sigma-Aldrich, in PBS) and gently inverting ...
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bioRxiv - Microbiology 2022Quote: ... and blocked for 3 h at 4°C in 60 ml blocking buffer composed of TBST buffer supplemented with 5% (w/v) BSA (Sigma Life Science). During the blocking stage ...
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bioRxiv - Neuroscience 2021Quote: ... 500 μl of the sample was mixed with 750 μl of 50%(w/v) iodixanol (made by mixing 4 ml of OptiPrep™ gradient (Sigma-Aldrich) and 0.8 ml of diluent [150 mM KCl ...
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bioRxiv - Bioengineering 2020Quote: ... HMPs were incubated with either a single or dual growth factor solution (Figure 1C: G1, G2) in 1 mL of 0.1% (w/v) bovine serum albumin (BSA; Millipore Corporation, Billerica, MA) in PBS ...
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bioRxiv - Microbiology 2023Quote: ... cultures were grown in 4% w/v clarified V8 broth supplemented with 30 µg/ml β-sitosterol (Sigma-Aldrich, St. Louis, Missouri) and incubated in the dark at 22 °C for 4 to 6 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... and ABTS substrate solution (water containing 0.54mg/mL w/v 2’2-Azinobis (3-ethylbenzthiazoline Sulfonic Acid) diammonium salt (Sigma-Aldrich, Cat#A-1888), and 0.1M Citric Acid and 0.03% v/v Hydrogen Peroxide (H2O2) ...
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bioRxiv - Neuroscience 2024Quote: ... at molar ratio of 4:2:2 was prepared by dissolving the lipids to a final concentration of 10 mg/mL in ethanol containing 2.3 % Span 80 w/v (Millipore Sigma cat# S6760). The lipid:RNA volume ratio was 3:1 ...
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bioRxiv - Cell Biology 2020Quote: Cells were grown at 23°C to 6×106-1×107 cells/ml in synthetic medium before addition of cycloheximide (Sigma Aldrich, 100 mg/ml stock in 100% ethanol) to 100 µg/ml final concentration ...
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bioRxiv - Bioengineering 2023Quote: ... was added to 1 mL of BG-11 in a 2 mL Eppendorf tube and supplemented with either 1 mM theophylline (Sigma-Aldrich; 100 mM stock dissolved in 100% DMSO) or 1% DMSO as a control and allowed to incubate for 3 days under 50 μmol photons m-2 s-1 prior to conducting the ABTS assays ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Zoology 2023Quote: ... was coated with 100 μl of either 10 or 100 mM caffeine (CAS: 58-08-2, Biopack, Buenos Aires, AR) or quinine (CAS: 6119-47-7, Sigma-Aldrich, St Louis, US) dissolved in distilled water ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Biochemistry 2020Quote: Several mL of a mixture consisted of 15% acetonitrile and 85% water with the addition of trifluoroacetic acid (TFA, Sigma-Aldrich, USA) to pH 2.5 to suppress dissociation of labile protons were added to the dried extract ...
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bioRxiv - Cell Biology 2022Quote: ... the patterned surface was incubated for 1 h with a mixture of 50 μg/mL fibronectin (Sigma-Aldrich, St. Louis, MO, USA), 5 μg/mL concanavalin A (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The reactants were dissolved in 25 mL of a solvent mixture consisted of 80% DMSO and 20% tert-amyl alcohol (Sigma Aldrich Co.) in which 1 g of 3 Å molecular sieves was added and the mixture was sonicated for 20 min ...
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bioRxiv - Immunology 2023Quote: ... The medium was removed and replaced with 1 mL of a mixture of plaque media and 2.0% agarose (Sigma, St. Louis, MO). Plaque media contained MEM ...
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bioRxiv - Neuroscience 2023Quote: Striatal neurons were plated on coverslips coated either with a mixture of 0.5 mg/ml PDL solution (Sigma Aldrich, St. Louis, USA), 1 mg/ml collagen solution (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted to a starting OD600 of 0.01 in 3 mL of BHIS supplemented with increasing concentrations of 1) bile salt mixture (50% cholate and 50% deoxycholate; Sigma-Aldrich B8756), 2 ...
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bioRxiv - Cell Biology 2024Quote: 3T3-L1 cells were grown in 6-well plates until 50-60% confluency before the medium was replaced by the concentrated virus mixture supplemented with 8 µg/mL polybrene (Sigma-Aldrich, #107689). After overnight incubation the medium was replaced by DMEM supplemented with 10% CS ...
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bioRxiv - Plant Biology 2024Quote: ... Each final inoculum consisted of 10 mL of the resuspended pellet in 1 L 0.5X Murashige and Skoog basal salt mixture (MS; Sigma-Aldrich, Darmstadt, Germany). A control treatment was made by adding 10 mL sterile 1x PBS into 1 L 0.5X MS.
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bioRxiv - Synthetic Biology 2021Quote: ... with synthetic complete medium (ForMedium LoFlo yeast nitrogen base CYN6510 and Formedium complete supplement mixture DCS0019) except for histidine competition experiment where histidine drop-out amino-acid mixture was used (Sigma Y1751) and complemented with desired levels of histidine (Sigma 53319).