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Citations for Millipore Sigma :
5101 - 5150 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized by isofluorane (Piramal) or avertin (2, 2, 2-Tribomoethanol, Sigma) and transcardially perfused by phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside (benzyl-GalNAc; Sigma), for three days ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were stained with 1 μg/ml 4′,6-Diamidino-2-phenylindole dihydrochloride in distilled water (D8417; Sigma-Aldrich, St. Louis, MO, USA), cover-slipped ...
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bioRxiv - Immunology 2022Quote: ... Perturbation profiling of the use of metabolic pathways by T cells was achieved by the addition of oligomycin (O4876; 1 µM), Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920; 2 µM) and rotenone (R8875; 1 µM - all from Sigma Aldrich, St. Louis, MO). Metabolic parameters were then calculated based on the following formulas:
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... airway mechanics were measured by challenging pigs to increasing concentrations of methacholine hydrochloride (MeCh) (0, 0.5, 1, 2, 4, 8, 16, and 32 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) via an Aeroneb™ nebulizer (Aerogen Ltd ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Genomics 2022Quote: ... slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich, St. Louis, MO, USA) in antifade based on DABCO (1,4-diazabicyclo(2.2.2)-octane ...
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bioRxiv - Physiology 2024Quote: ... Nuclear stains used in this study were 4’,6-diamidino-2-phenylindole (DAPI; final concentration 1 μg/ml Sigma-Aldrich, St. Louis, MO, USA). F-Actin was stained with Alexa Fluor 568 phalloidin (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were used for purification of recombinant proteins by 2 h incubation with GST-agarose beads at 4 °C according to manufacturer’s protocol (Sigma Aldrich, St. Louis, USA, G4510). SDS-PAGE electrophoresis was used to check level of expression and purity of purification.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then stained with a 1X crystal violet solution for 20 minutes at RT (2% crystal violet, Sigma; 20% ethanol; 4% formaldehyde, Merck). Plates were washed with clear water and plaques were counted manually ...
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bioRxiv - Microbiology 2023Quote: ... The membrane pellets were solubilized in 4 mL TBS buffer (50 mM Tris-HCl, 150 mM NaCl, pH = 7.4) with 2% Triton X-100 (Sigma-Aldrich, St Louis, MO, USA). The solubilized membrane-fraction was loaded on a column containing 1 mL anti-DYKDDDDK (Flag ...
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bioRxiv - Immunology 2023Quote: ... then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat. No. S1888) in PBS for 48 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The blots were probed with the following primary antibodies at room temperature for 1-2 hours or at 4 ℃ overnight: anti-GAPDH (Millipore, #MAB374, 1:5,000 dilution), anti-Flag M2 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were placed on glass slides (Matsunami, Osaka, Japan) and stained with 4’,6-diamidino-2-phenylindole (DAPI; D9542, Sigma-Aldrich, St. Louis, MO) for 5 min ...
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bioRxiv - Zoology 2023Quote: ... All wells were then washed with embryo media three times before being incubated on an orbital incubator shaker at 130 rpm and 28°C for 15-20 minutes in media containing 0.01% 2-[4-(dimethylamino) styryl]-1-ethylpyridinium iodide (DASPEI, Sigma Aldrich; cat. #3785-01-1) to selectively stain live neuromast hair cells ...
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bioRxiv - Microbiology 2023Quote: ... and then cells were washed with PBS supplemented with bovine serum albumin at 1 g/l and incubated with 12 concentrations of mACE2 (4 pM to 10 nM, dilution series with factor 2) and 20 nM bilirubin (Sigma-Aldrich, Cat# 14370-1G). Performance comparison with Expi293F cells produced ACE2 peptidase domain (residues 18–617 ...
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bioRxiv - Plant Biology 2023Quote: ... and SIX6 with an internal TEV protease cleavage site for pro-domain removal were processed with 2 to 4 units of thrombin from bovine plasma (600-2,000 NIH units/mg protein) (Sigma-Aldrich Inc., St. Louis, USA) per mg of protein at 4°C or TEV protease (produced in-house ...
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibody was washed with PBS++ including 4’,6-diamidino-2-phenylindole for initialization (DAPI; 1:10 000, Sigma Aldrich, St. Louis, MS, USA). Where needed ...
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bioRxiv - Cancer Biology 2022Quote: ... After 2 hours medium was replaced with 2 mL medium containing 2 % FBS and 2 mg/mL polybrene (Sigma). After an hour this medium was removed and replaced with 2mL of medium containing 5x 106 IU of lentiviral vector ...
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bioRxiv - Molecular Biology 2021Quote: ... heterozygote (HNF4α fl/+, Cre/+), and knockout (HNF4α fl/fl, Cre/+) mice (N=3-6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
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bioRxiv - Cell Biology 2023Quote: ... ∼1 kb 5’ homology arm and ∼1 kb 3’ homology arm of msps genomic region were amplified from the genomic DNA (Sigma, Extract-N-Amp™ Tissue PCR Kit), mutated to be insensitive to gRNAs ...
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bioRxiv - Microbiology 2020Quote: ... BLT-4 (SML0512, Sigma) (both prepared in pure DMSO ...
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bioRxiv - Microbiology 2020Quote: ... 4 mM MgCl2 (Sigma) and 12.5 µl of JumpStart Ready Mix (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... 4 mM MgCl2 (Sigma), 0.25 µl FITC (1:1000) ...
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bioRxiv - Developmental Biology 2020Quote: ... 4) Putrescine (Sigma P5780) – 16 µg/ml final concentration ...
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bioRxiv - Neuroscience 2022Quote: ... Sigma Aldrich)/sucrose (4%, Sigma Aldrich) for 10 min at room temperature and incubated with primary antibodies (α-TSPAN5 ...
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bioRxiv - Plant Biology 2021Quote: ... or 4% BSA (Sigma) in TBST ...
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bioRxiv - Biochemistry 2020Quote: ... 4°C (Sigma 4K15C) and the pellet was resuspended in 60-75 mL of lysis buffer ...
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bioRxiv - Bioengineering 2020Quote: 4% paraformaldehyde (Sigma-Aldrich) was used to fix hNTOs for 2 hours followed by washing with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM CuSO4 (Sigma), and 100 mM ascorbic acid (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... 4 mM phosphoenolpyruvate (Sigma), 2.5 µL of PK/LDH (PK 600 U/mL – 1000 U/mL ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μg ubiquitin (Sigma), and 2 μL substrate (His6-HA3-ABF2) ...
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bioRxiv - Plant Biology 2020Quote: ... or 4 % BSA (Sigma) in TBS with 0.1 % tween 20 ...
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bioRxiv - Microbiology 2020Quote: ... in 4% ethanol (Sigma) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 4 Mg-ATP (Sigma); 0.3 Na-GTP (Sigma) ...
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m7G cap-eIF4E interaction stimulates polysome formation by enhancing first-round initiation kineticsbioRxiv - Biophysics 2021Quote: ... 4) Cy3-αFLAG (Sigma A9594 ...
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bioRxiv - Neuroscience 2020Quote: Tamoxifen (4-OHT) (Sigma) was resuspended at 20 mg/ml in ethanol and diluted into sunflower seed oil at a final concentration of 2 mg/ml in 10% of ethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-hydroxytamoxifen (Sigma H7904) was used at 500nM ...
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bioRxiv - Cell Biology 2021Quote: ... 4-OH TMX (Sigma) (1 μM ...
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bioRxiv - Biophysics 2020Quote: ... 4-TMX (Sigma H6278) was first dissolved in ethanol to a concentration of 20 mg mL−1 ...
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bioRxiv - Molecular Biology 2022Quote: ... 4% CHAPS (Sigma-Aldrich) was added additionally in IP Lysis Buffer.
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bioRxiv - Neuroscience 2022Quote: ... with 4% PFA (Sigma) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 4-thiouracil (4tU, Sigma) was dissolved in NaOH and added to cells at final concentration of 5 mM 4tU for the indicated times (6-10 minutes) ...
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bioRxiv - Plant Biology 2022Quote: ... 4 triazole (3AT, Sigma) or with GA to promote interaction between DELLA and GID1.
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bioRxiv - Biophysics 2022Quote: ... Formaldehyde solution 4% (Sigma) was used for fixation for 15 min at RT followed by 3 washes ...
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bioRxiv - Developmental Biology 2022Quote: ... 4% 7-DHC (Sigma)) and 50-200 μL fresh baker’s yeast as food source and egg laying substrate on top ...
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bioRxiv - Neuroscience 2022Quote: ... and 4% MMTS (Sigma), adjusted to pH 7.5 and sonicated ...
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bioRxiv - Genomics 2022Quote: ... 4-thiouracil (4tU) (Sigma) was dissolved in NaOH(83mM) ...
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bioRxiv - Cancer Biology 2022Quote: ... 4-Hydroxytamoxifen (Sigma, H7904), doxycycline (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... containing 4% digitonin (Sigma) and protease-inhibitor cocktail (Sigma) ...