Labshake search
Citations for Millipore Sigma :
5101 - 5150 of 10000+ citations for Cow Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2023Quote: ... Overnight incubation with primary antibodies diluted in blocking/permeabilisation buffer (GFP – 1:1000 Ab13970 Abcam, TH – 1:1000 Ab152 Sigma, IL33 – 1:50 AF3626 R&D) were performed overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... 2.76 µL master mix 1 (1 µL 5x Superscript II reaction buffer [ThermoFisher], 1 µL 5 M betaine [Sigma-Aldrich], 0.25 µL 100 mM dithiothreitol (DTT), 0.035 µL 1 M magnesium chloride [ThermoFisher] ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by a 2 h incubation at 37 °C with the respective primary antibody (anti-G3BP2, 1:600 [Proteintech®, 16276-1-AP]; anti-GAPDH-AF488 [Sigma-Aldrich, MAB374-AF488], 1:500). Cells were washed and incubated with secondary antibody (mouse anti-rabbit IgG-CFL 647 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 50 μg/ml−1 of propidium iodide and 50 μg/ml−1 of RNase A (Sigma). Dissociated cells were left at room temperature for 20 mins ...
-
bioRxiv - Cell Biology 2020Quote: ... and simultaneous binding of FITC-labeled BS-1 lectin (BS-1) (Sigma Diagnostics, St. Louis, MO). SECR ...
-
bioRxiv - Cell Biology 2020Quote: ... and simultaneous binding of FITC-labeled BS-1 lectin (BS-1) (Sigma Diagnostics, St. Louis, MO). SECR ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse anti-γ-tubulin (GTU88; 1:1000 for IF; 1:10,000 for WB; Cat#T6557, Sigma), rabbit or guinea pig anti-Cnn (1:1000 for IF ...
-
bioRxiv - Cell Biology 2020Quote: ... they were anaesthetized with E3 solution/0.16 mg/mL tricaine/1% 1-phenyl-2-thiourea (Sigma) and embedded in 0.5-0.8% low melting agarose ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT and 0,5% NP-40 supplemented with 1:300 mammalian protease inhibitor cocktail (Sigma). FCS measurements were performed using a Zeiss LSM780 confocal microscope using a C-Apochromat 40x/1.2 W Corr M27 objective and using a water-phase immersion oil Immersol W 2010 (Zeiss) ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT and 0,5% NP-40 supplemented with 1:300 mammalian protease inhibitor cocktail (Sigma). Cell lysates were subsequently subjected to FCCS and treated with 100 μg/ml RNase A (DNase and protease-free ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT and 0,5% NP-40 supplemented with 1:300 mammalian protease inhibitor cocktail (Sigma). Cell lysates were briefly centrifuged at 800 xg for 1 minute and supernatant was transferred to a 35 mm glass bottom dish (No ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT and 0.5% NP-40 supplemented with 1:300 mammalian protease inhibitor cocktail (Sigma) on ice ...
-
bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-V5 (PRB-189P, Covance, 1/1000) or mouse anti-FLAG (M2, Sigma, 1/1000) antibodies ...
-
bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies for cilia (mouse anti-acetylated tubulin, 1:1000 dilution, Sigma, cat# 6-11B-1) were diluted in block buffer and incubated on slides for 2hr at RT or overnight at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... and mouse anti-acetylated α-tubulin (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) were diluted in 5% normal goat serum (NGS ...
-
bioRxiv - Developmental Biology 2022Quote: ... and mouse anti-acetylated α-tubulin (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) were diluted in 5% normal goat serum (NGS ...
-
bioRxiv - Neuroscience 2022Quote: ... and Armenian hamster anti-PECAM-1 (CD31; Millipore Sigma, cat no. MAB1398Z, clone 2H8; 1:250). Cells were washed again three times with 1X PBS and incubated with secondary antibodies in 3% BSA for 45 min at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... blots were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3000 or 1:10000, Sigma) and were then developed in Chemidoc gel apparatus (Biorad ...
-
bioRxiv - Immunology 2022Quote: ... 1% penicillin-streptomycin and 1% L-glutamine) with 10 units/ml of DNAse I (Sigma-Aldrich) at 37°C and 5% CO2 ...
-
bioRxiv - Genomics 2020Quote: ... 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022, Sigma-Aldrich)) ...
-
bioRxiv - Microbiology 2020Quote: Antibodies against LeishIF4E2(rabbit polyclonal, 1:2,000) and against the SBP tag (Millipore, monoclonal, 1:10,000), were used to detect the endogenous and tagged LeishIF4E2 proteins ...
-
bioRxiv - Immunology 2021Quote: ... dissolved in PBS and emulsified at a 1:1 ratio with Incomplete Freunds Adjuvant (Sigma Aldrich). For the CD8+ T cell SLPs ...
-
bioRxiv - Cell Biology 2019Quote: ... For BN-PAGE samples were lysed in 1 % digitonin buffer (1 % digitonin (Sigma/Merck, Darmstadt, Germany) in 20 mM Tris–HCl ...
-
bioRxiv - Genetics 2019Quote: ... and 1:1000 chemically defined lipid supplement) to which 1 μM all-trans retinoic acid (Sigma) was added until d100 ...
-
bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
-
bioRxiv - Neuroscience 2021Quote: ... Lysates were incubated O/N with prewashed 1:1 anti-HA agarose beads (Sigma, Cat# A2095) at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were against β-III tubulin (Tuj-1; mouse anti-rat, 1:300; Millipore), choline acetyltransferase (ChAT ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 μl of 50 mM freshly prepared CuSO4·5H2O in H2O (final concentration 1 mM, Sigma), 1 μl of 50 mM freshly prepared TCEP (final concentration 1 mM ...
-
bioRxiv - Neuroscience 2021Quote: ... mouse α-tubulin clone B-5-1-2 (1:1000; Sigma Aldrich, San Luis, MO, U.S.). For immunofluorescence primary antibodies were labeled with Alexa-conjugated secondary antibodies Alexa 488 ...
-
bioRxiv - Cell Biology 2021Quote: ... 200μl 5X RPMI (1:1 mixture of 10X RPMI and tissue culture (TC) water) (Sigma #R1145) and 25μl 1M NaOH (Sigma #S5881 ...
-
bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by incubation overnight with primary antibodies (1:200, Nucleolin, CST, 145745; 1:400, NPM1, Sigma, B0556 ...
-
bioRxiv - Plant Biology 2021Quote: ... α-naphthaleneacetic acid (500 µL; 1 mg/mL) and kinetin (50 µL; 1 mg/mL; Sigma) were added and the pH of the mixture was adjusted to 5.7 with aqueous KOH before dilution to 1000 mL with H2O ...
-
bioRxiv - Microbiology 2020Quote: ... albicans cells were washed three times in PBS and 1 x105 J774A.1 macrophages (Sigma, UK) were infected with 5 x 105 yeast cells (multiplicity of infection [MOI] of 5 ...
-
bioRxiv - Plant Biology 2020Quote: ... supplemented with 10 g L−1 sucrose and 3 g L−1 phytagel (Sigma, Steinheim, Germany) or 8 g L−1 agar (Winkler ...
-
bioRxiv - Microbiology 2020Quote: ... 1 mg ml-1 of 3–5 kDa fluorescein isothio-cyanate (FITC)-dextran (Sigma-Aldrich, Germany) in phenol-red free DMEM/F12 medium (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2021Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS and 200 ug/ml Digitonin (D141-100MG ...
-
bioRxiv - Cell Biology 2021Quote: ... and 5 U ml−1 penicillin and 50 μg ml−1 streptomycin (Merck Life Science (Sigma)) at 37 °C and 5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: ... Embryos were incubated with acetylated tubulin antibody (clone 6-11B-1, MABT868, Sigma-Aldrich, 1:1000) overnight at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... phosphatase inhibitors (1 mM sodium fluoride [201154; Sigma-Aldrich], 1 mM sodium orthovanadate [S6508; Sigma-Aldrich] ...
-
bioRxiv - Neuroscience 2022Quote: ... The cleaned dishes were incubated with 1 mL of 1% (3-aminopropyl) triethoxysilane (APTES; Sigma Aldrich) in ethanol for 1hour at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... 29] for astrocytes (rabbit anti-GFAP 1:500, Dako, mouse anti-α-tubulin 1:500, Sigma) and microglia (rabbit anti-Iba1 ...
-
bioRxiv - Genomics 2022Quote: ... 4% NP-40) supplemented with 1 mM PMSF and 1:100 dilution of PIC (Sigma P8340) per 10×106 cells ...
-
bioRxiv - Bioengineering 2022Quote: ... primary antibodies were prepared at 1:100 in a 1:4 blocking solution (2% BSA (Sigma), 10% normal goat serum (Life Technologies ...
-
bioRxiv - Bioengineering 2022Quote: ... 1% (w w-1) phenylbis(2,4,6-trimethylbenzoyl) phosphine oxide (Irgacure 819, 415952, Sigma-Aldrich, MO, USA) as the photo-initiator ...
-
bioRxiv - Physiology 2020Quote: ... diluted with dH2O (1:5) and BSA (bovine serum albumin, 1 mg/ml) (Sigma Chemicals, USA) as a standard ...
-
bioRxiv - Biochemistry 2021Quote: ... and then re-suspended in 1 mL RNAse solution (1 mg/mL RNAse A [Sigma-Aldrich] in 50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Neuroscience 2021Quote: ... and guinea pig anti-vGlut2 antiserum (1:12,000, cat# AB2251-1, Millipore Sigma, Temecula, CA, USA) in PBS-T containing 2% normal donkey serum ...
-
bioRxiv - Neuroscience 2021Quote: ... Sarkospin supernatants or pellets fractions were treated or not with 1 μg.ml-1 Proteinase K (Sigma) from 0 to 60 minutes at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 µl of a solution of muscimol hydrobromide (1 µg in aCSF, G019 Sigma-Aldrich, Canada), or aCSF (Tocris Bioscience ...