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Citations for Millipore Sigma :
5051 - 5100 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... FLAG-IP lysis buffer (50 mM HEPES pH 8.0, 100 mM KCl, 2 mM EDTA, 0.1% NP40, 10% glycerol, 1 mM PMSF, 1 mM DTT, and Sigma protease inhibitors P8340 1:500). Resuspended lysates were first froze and thawed twice (dry ice and 37 °C waterbath ...
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bioRxiv - Cancer Biology 2022Quote: ... After 2 hours medium was replaced with 2 mL medium containing 2 % FBS and 2 mg/mL polybrene (Sigma). After an hour this medium was removed and replaced with 2mL of medium containing 5x 106 IU of lentiviral vector ...
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bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
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bioRxiv - Cancer Biology 2021Quote: ... transduced cells were selected by incubating the monolayer in DMEM:F12 (1:1)+10% FBS containing 2 μg/mL Puromycin dihydrochloride (Sigma, USA) for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Cas9-sgRNA vector and a control vector (pEGFPN1) in the ratio: 2:1:1 using PEI (18) from Sigma Aldrich, Inc ...
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bioRxiv - Biochemistry 2019Quote: ... were amplified by PCR from genomic DNA and cloned into the multiple coning sites (MCS) 1 and 2 using the NcoI/NotI and NdeI/XhoI restriction sites of a modified pETDuet-1 vector (Novagen) containing an N-terminal PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... 400 mM KCl, 2 mM MgCl2, 0.002% Brij35, 1 mM β-mercaptoethanol, 1 mM imidazole, 50 mM L-Arginine, Sigma#A5006 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was lysed in lysis buffer (50 mM Tris-HCl, pH 8, 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F] ...
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bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
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bioRxiv - Cell Biology 2020Quote: ... For AsiSI or I-PpoI nuclease induced DSBs, cells were treated with Shield-1 (1 μM, AOB1848, Aobious) and 4-OHT (2 μM, H7904, Sigma) for 5 hours before washing with PBS and adding back media.
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bioRxiv - Plant Biology 2021Quote: ... 1 g of the infiltrated leaf tissue was ground in 4 mL of extraction buffer (1/2 tablet of Sigma Complete™ mini protease inhibitor (per 15 mL) ...
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bioRxiv - Microbiology 2021Quote: Test sera were heat-inactivated at 56°C for 30 minutes to destroy any complement activity and serially diluted 1:2 in cell culture media containing 1% foetal calf serum (Sigma). Virus was diluted to give 100-250 foci in the virus-only control and then added to the serum dilutions before incubation for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... then added to cells and incubated for 2 hours before discarding and adding a 2.4% (w/v) microcrystalline cellulose and 2% FCS (mixed 1:1) overlay (Sigma-Aldrich). The assay was incubated at 37°C and 5% CO2 for 70 hours then fixed in 4% PFA for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were rinsed in de-ionized water and re-fixed in freshly made Phosphomolybdic/Phosphotungstic/dH2O (1:1:2) acid solution for 8 min (#HT153, #HT152, Sigma), and then they were stained in Aniline Blue solution for 5 min (#HT154 ...
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bioRxiv - Developmental Biology 2022Quote: ... to induce brain regional patterning, EBs were treated individually with SAG (1:10,000) (Milli- pore, 566660) + IWP-2 (1:2,000) (Sigma-Aldrich, I0536) for ventral identity and with cyclopamine A (1:500 ...
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... These were mounted in a drop of in 0.1 M phosphate buffer containing 1:1 of 2% methyl cellulose (Sigma-Aldrich) and 2.3 M sucrose on TEM grids with a carbon-coated Formvar film (Taab Laboratory Equipment Ltd) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EDTA and 2 mM PMSF) and eluted with 1 mg/ml FLAG® Peptide (Sigma, cat. no. F3290) in wash buffer overnight on a rotator at 4°C for anaylsis by mass spectrometry.
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Developmental Biology 2021Quote: ... 70nm Sections were picked up using a 1 :1 mixture of 2.3M sucrose and 2% methyl cellulose (cat# M-6385, Sigma-Aldrich) as a pick-up solution and sections were transferred to carbon and formvar coated mesh grids (cat# H100-Cu ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated with rabbit anti-TMEM43 polyclonal antibody (1:100, NBP1-84132, Novus) (Ab1-2) and mouse anti-FLAG (1:500, F1804, Sigma) for overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 1 - 2 µl of solutions containing 0.1 or 1% of the non-ionic detergent Triton X-100 (TrX; Sigma-Aldrich), dissolved in 0.1 M phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were treated with 1 x 10-5 M 5-fluoro-2-deoxy-uridine and with 1 x 10-5 M uridine (Sigma) for 3 days ...
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bioRxiv - Molecular Biology 2023Quote: ... These cultures were then sub-cultured (1:100 dilution) in 2 mL of TYG medium containing doxycycline (0.016 µg mL-1, Sigma-Aldrich), tetracycline (0.05 µg mL-1 ...
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bioRxiv - Immunology 2022Quote: ... murine lung and liver tissue samples were transferred into 2 ml tubes containing 1 mL of digestion media (1 mg/mL Collagenase D (Sigma 11088882001 ...
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bioRxiv - Microbiology 2022Quote: ... was previously isolated on the International Streptomyces Project 2 medium (ISP2, dextrose 4 g.L-1 [Sigma-Aldrich, St. Louis, MO, USA], yeast extract 4 g.L-1 [Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in appropriate secondaries diluted in 2% bovine serum in PBS for 1 h at 20-25°C and counterstained with Hoechst 33257 (1:1000; Sigma). For brain tissue incubated in anti-INO80 antibody (ab118787 ...
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bioRxiv - Microbiology 2024Quote: ... the coupling of the PEG-acetal linker with HPV16 or fcHPV16 PsV particles was performed by immersing the PEG-coated cantilevers (∼1-to-2) in (w/v) 1% citric acid (Sigma) in milli-Q water for 10 mins ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of each culture was removed and mixed 1:1 with freshly made 2% formaldehyde in PBS (Sigma-Aldrich) and stored at 4°C for analysis by flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of each culture was removed and mixed 1:1 with freshly made 2% formaldehyde in PBS (Sigma-Aldrich) and stored at 4°C for analysis by flow cytometry ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-MBP (1:500; Serotec) antibodies (OPCs) or 2 h incubation with mouse anti-GFAP (1:200, Sigma) (astrocytes) ...
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bioRxiv - Plant Biology 2023Quote: ... fresh sporangia produced after crossing T0 Mprop mutants to wild-type (Tak-1 or Tak-2) were sterilised in 1 % (w/v) sodium dichloroisocyanurate (Sigma) solution for 3 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... the seeds were sowed on half strength Murashige and Skoog (1/2 MS) media with 0.8% sucrose and 1% Phytagel (Sigma-Aldrich) and kept the plates in the dark at 4 °C for 2 days for stratification treatment ...
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bioRxiv - Biochemistry 2023Quote: ... Flow chambers were prepared with the following steps: 1) incubated for 2 min with block containing 25 μl of 1% Pluronic F-127 (Sigma), 50 μg/ml casein in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...
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bioRxiv - Cancer Biology 2023Quote: ... Uniprot P50750-1) and Cyclin T1 (residues 2-259; Uniprot O60563-1) were synthesized and cloned into pFastBac dual (Sigma). Each open reading frame codes for N-terminal 6-His tags and TEV cleavage sites ...
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bioRxiv - Molecular Biology 2024Quote: ... 1/2.5/1 (v/v/v)) containing 10 µl of 2-isopropylmalic acid (0.5 mg/ml, Sigma-Aldrich, Tokyo, Japan) as an internal standard (IS) ...
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bioRxiv - Microbiology 2024Quote: ... α-tubulin was detected as a loading control with the mouse monoclonal antibody B-5-1-2 (Sigma, T5168, 1/4000) for 2 hrs at 25°C ...
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bioRxiv - Molecular Biology 2024Quote: ... They were then sowed on half strength Murashige and Skoog (1/2 MS) media containing 0.8% sucrose and 1% Phytagel (Sigma-Aldrich), followed by a stratification treatment at 4 ℃ in the dark for two days ...
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Dopamine depletion can be predicted by the aperiodic component of subthalamic local field potentialsbioRxiv - Neuroscience 2021Quote: ... 4 or 8 μl of 6-OHDA (5 μg in 1 μl saline containing 0.1% ascorbic acid; Tocris, Bristol, ENG and Sigma-Aldrich, MA, USA) was injected into the left MFB at the following coordinates ...
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bioRxiv - Bioengineering 2020Quote: ... and 800 mg L-1 humic acids (CAS No. 1415-93-6) were prepared by adding in ultrapure water (Sigma Aldrich, UK).
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bioRxiv - Cell Biology 2021Quote: ... lentiviral particles were generated by transfecting HEK293T cells with 6 μg PLKO.1 scramble control and MISP-targeted shRNA plasmids (SIGMA; TRCN0000422523, TRCN0000116527), 4 μg psPAX2 packing plasmid (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected 6 days after plating in 1 ml PBS and incubated with 60-80 ul collagenase IV [10mg/ml] (Sigma #C5138-1G) at 37 C for 10 mins ...
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bioRxiv - Biochemistry 2020Quote: ... cells were fixed using a 6% (v/v) formaldehyde: phosphate-buffered saline solution and stained with 1% (w/v) crystal violet (Sigma-Aldrich, Italy) in 70% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
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bioRxiv - Immunology 2019Quote: ... duplicate wells of a 12-well plate were seeded with 1 ml/well of the cell suspension (1 × 106 cells/ml) and virus was added in the presence of 6 μg/ml polybrene (Sigma-Aldrich, #107689) (multiplicity of infection of 25) ...
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bioRxiv - Cancer Biology 2019Quote: ... 107 cells cultured with 100nM 4-OHT or ethanol control for 6 or 24 hours were incubated with 1% formaldehyde (Sigma-Aldrich, F8775) at room temperature for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Th-1 and Th-17 cells were restimulated for 6 hr in the presence of PMA (100 ng/ml, Sigma, Cat#P8139), Ionomycin (1 μM ...