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Citations for Millipore Sigma :
5051 - 5100 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: SK-N-SH-N cells were plated onto 6-well tissue culture plates coated with poly-L-lysine (Sigma-Aldrich). Cells were plated at 3×105 cells/ml in MEMα medium containing a very low-serum level (2% FBS) ...
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bioRxiv - Neuroscience 2023Quote: ... Adult female SNSiDTR (n=6) and TrkBiDTR (n=19) mice were injected i.p with 40 μg/kg DTX (Sigma, D0564) with a second dose after 72 h ...
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bioRxiv - Neuroscience 2019Quote: Female fly heads (30-50 per N) at 2 DAE (days after eclosion) were homogenized using a mechanical pestle homogenizer in 200μl Trizol (Sigma). After adding 800μl Trizol with 200μl chloroform and a 15min centrifugation at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Antibodies were diluted to 17.5 μg/ml in 100 μl 0.1M 2[N-Morpholino]ethanesulfonic acid (MES)-buffer (M2933, Sigma-Aldrich), pH 4.5 ...
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bioRxiv - Bioengineering 2020Quote: ... O,O′-bis[2-(N-succinimidyl-succinylamino)ethyl]polyethylene glycol with molecular weight of 2kDa (NHS-PEG-NHS, Sigma) was mixed with the protein solution at 10 w/w% ...
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bioRxiv - Immunology 2022Quote: ... cells using minimal essential medium supplemented with 0.2% bovine serum albumin and 2 μg/mL N-Tosyl-L-phenylalanin-chlormethylketon (Sigma). For the determination of the tissue culture infectious dose 50 (TCID50) ...
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bioRxiv - Developmental Biology 2019Quote: ... Denuded fail to fertilize M-II oocytes (n=8) were obtained 36h post-ovulation and cultured briefly (2-4h) in 50µl KSOM medium (Millipore) containing 0.4% BSA at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Group 2 (DN+CL, n=8): mice received surgical denervation of BAT and intraperitoneal injections of CL316243 (C5976; Sigma) at 1mg/kg for consecutive 14 days ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Oligonucleotides 5’-GAAAAAAAAAATATACGCTAAGATTTTTGG-3’ and 5’-ATGACTAAACCCCCCCTCC-3’ synthesized and HPLC-purified by Sigma-Aldrich were used ...
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bioRxiv - Physiology 2024Quote: ... CDH1 Forward 5′-TTACTGCCCCCAGAGGATGA-3′ and Reverse 5′-TGCAACGTCGTTACGAGTCA-3′;) were purchased from Sigma-Aldrich. mRNA expression was determined using the comparative 2-ΔΔCt method and normalized to the mRNA expression level of endogenous references (PPIA ...
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Micro topographical instruction of bacterial attachment, biofilm formation and in vivo host responsebioRxiv - Microbiology 2022Quote: ... A mould fluorosilane release agent, trichloro (1H, 1H, 2H, 2H-perfluorooctyl) silane (Sigma Aldrich) was applied using vapour deposition ...
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bioRxiv - Bioengineering 2022Quote: ... and coated with trichloro[1H,1H,2H,2H-perfluorooctyl]silane (Sigma-Aldrich, Catalog 448931).
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bioRxiv - Cell Biology 2019Quote: ... the master mold was silanized with trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma) for easier lift-off ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently treated overnight with Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma 448931). The next day ...
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bioRxiv - Immunology 2021Quote: Blood was extracted from hBLT mice at 3 and 5-6 weeks post-infection and lysed with Red Blood Cell Lysis Buffer (SIGMA). T cells were activated for 1.5 h with 5μl/ml of anti-CD28 and anti-CD49d in the presence or absence of 6.4μg/ml of a Gag pool of peptides in the presence of 0.5μg/ml Brefeldin A ...
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bioRxiv - Immunology 2020Quote: ... Cells were then treated with 6-thio-dG (3-5 μM) in the presence of 500 ng/ml doxycycline (Sigma), washed three times with warm PBS and allowed to recover in regular growth medium containing 500 ng/ml doxycycline ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Immunology 2020Quote: ... the Jurkat cells were transduced with TCR lentivirus at an estimated multiplicity of infection (MOI) of 5 with 1 μl of polybrene at a final concentration of 4 μg/ml (Sigma, St. Louis, MO). Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 5% BSA diluted in PBS before being incubated overnight at 4°C with rabbit ɑ-MBNL1 antibody (1:1000, Millipore Sigma, ABE241) or mouse ɑ-RFP antibody (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 cells were infected with the corresponding lentiviruses (1/5 – lentivirus/cell culture medium) in the presence of 4 μg/mL polybrene (Sigma-Aldrich, cat# 107689). 4 days post-infection ...
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bioRxiv - Synthetic Biology 2024Quote: ... cat # K3007) and 3-O-C12-HSL (N-(3-Oxododecanoyl)-L-homoserine lactone from Sigma-Aldrich, cat # O9139 ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies], 2 µg/mL heparin [Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Bioengineering 2022Quote: ... cell-seeded wells (n=3 per group) were rinsed with PBS and incubated in 500 μL of 5% trichloroacetic acid (TCA, Sigma-Aldrich, T6399) in UPW for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell lines were regularly passaged every 3-4 days by dissociation with Trypsin-EDTA 0.25% (Sigma Aldrich). The cell lines were regularly subjected to monthly screening for mycoplasma infection ...
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bioRxiv - Neuroscience 2019Quote: ... cells at 3–4 DIV were incubated with culture medium containg 0–0.8 mM methylmethanesulfonate (MMS, Sigma, 129925) or 0–8 µM etoposide (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... tissues were washed 3 times in PBS and stored at 4°C in PBS containing 0.05% NaN3 (Sigma) until use ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissue sections were then treated with 3% H2O2 and nonspecific binding was blocked with 4% BSA (A7906; Sigma) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: Adult mice (3-4 months old) were injected intraperitoneally (i.p.) with 100 mg/kg of tamoxifen (Sigma-Aldrich) dissolved in 10% EtOH in corn oil (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... PerMacs were collected from peritoneal gavages 4 days after intraperitoneal injection of 3% brewer thioglycolate medium (B2551) (Sigma). Transfection of interferon-stimulatory DNA (ISD ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then treated with 3% H2O2 and nonspecific binding was blocked with 4% BSA (A7906; Sigma) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampi were first lysed in 20 volumes of homogenizing buffer (pH 7.3 solution of 0.32 M sucrose and 0.005 M Na3PO4) at 4°C supplemented with protease inhibitors (Sigma), 50 mM sodium fluoride ...
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bioRxiv - Cell Biology 2022Quote: Cultures were fixed in 4% paraformaldehyde and stained with antibodies against α-tubulin (Millipore MAB1864; 3 μg/mL), MUC5AC (ThermoScientific 45M1 ...
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bioRxiv - Cell Biology 2023Quote: The following chemicals were used in this study: indole-3-acetic acid (IAA, auxin) (87-51-4) (Millipore); 4-hydroxytamoxifen (4-OHT ...
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bioRxiv - Immunology 2023Quote: ... 4E7 cells were resuspended in 4 mL NK cell expansion media with 3 uM BX-795 (Sigma-Aldrich), and were left to incubate in a 37°C incubator for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and the slices were placed on Millicell membranes (3–4 slices per membrane, 0,4 µm Millicell, Merck Millipore). Slices were cultured in DMEM/F-12 with GlutaMax ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM BME) and concentrated to <4 ml using 100,000 kDa MWCO Amicon® Ultra spin concentrators (Millipore). Protein was loaded on a Superdex 200 pg 16/60 column (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: Leaves of 3-4-week-old Nicotiana benthamiana plants were agroinfiltrated and sprayed with 10 μM estradiol (Sigma) 24 h after infiltration to induce protein expression ...
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bioRxiv - Neuroscience 2021Quote: ... cells were subcultured onto glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5 mM DTT) and concentrated to ∼ 5 mg/mL (Amicon Ultra 4 mL Centrifuge filters, 30 KDa NMWL devices, Millipore Cat# UFC805024). Last ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were incubated for 6 h at 37 °C under a humidified atmosphere with 5% CO2 in the presence of 5 μM carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (Sigma-Aldrich, USA) to induce mitophagy ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T) followed by anti-Rabbit antibody (1:10,000 [Sigma] in 5% milk in PBS-T). The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T) ...
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bioRxiv - Immunology 2019Quote: ... adult animals (6-12 weeks) were perfused with 25 mL of PBS (Hyclone) and 25 mL of 4% paraformaldehyde (PFA, Sigma) sequentially at room temperature (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... and RANK-L (100 ng/ml) for 4 to 6 days followed by staining for Tartrate-resistant acid phosphatase (TRAP) using a kit (Sigma) per manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Cell Biology 2021Quote: NCMs were seeded at a density 4 or 8 × 105 cells/well in 6-well plates coated with 0.01% of collagen (Sigma-Aldrich) and cultured in a medium containing DMEM/Medium199 (4:1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Next the slides were mounted with a coverslip using Mowiol (6 g glycerol, 2.4 gr polyvinylalcohol 4-88 (Sigma, 81381), 6 ml MQ and 12 ml 0.2 M Tris HCL pH 8.5).
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bioRxiv - Physiology 2021Quote: ... 6 × 105 cells were seeded on 6-well multiwell plates and counted every two days from day 4 to day 14 using Trypan blue (Sigma) and a Neubauer chamber.