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Citations for Millipore Sigma :
5001 - 5050 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... human full length NHE1 was cloned into p3xFLAG-CMV-14 (Sigma-Aldrich) containing a C-terminal 3xFLAG tag ...
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bioRxiv - Immunology 2020Quote: ... and product intensity measured by Software ImageJ(83) and normalized to ACTIN amplicon products (5’-GACGACATGGAGAAAATCTG-3’ and 5’-ATGATCTGGGTCATCTTCTC-3’, Sigma-Aldrich, St. Louis, Missouri, USA).
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bioRxiv - Neuroscience 2021Quote: ... FW 5’ – CCA CAT GGG AGA GTC ACA T −3’ and RV 5’-ATA GCC TGG AAG CGG TCA GAT G −3’ (Sigma-Aldrich Japan, Inc., Tokyo, Japan). The PCR products (521 bp ...
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bioRxiv - Bioengineering 2022Quote: ... crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Carl Roth GmbH + Co. KG) and N-hydroxysuccinimide (NHS) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was applied ...
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bioRxiv - Microbiology 2022Quote: The AHL signaling molecules used in this study are N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL, Sigma-Aldrich CAS# 168982-69-2), N-butyryl-homoserine lactone (C4-HSL ...
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bioRxiv - Neuroscience 2022Quote: ... BrdU injections were done 24h before sacrifice for the analysis at 3 dpKA and 3/7 days post administration of zinc (Cat#83265-250ML-F, Sigma-Aldrich, St Louis, MO, USA). To identify newborn cells at 14 dpKA ...
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bioRxiv - Plant Biology 2024Quote: ... 2-(1H-Indol-3-yl)-4-oxo-4-phenyl-butyric acid (PEO-IAA; OlChemIm, Olomouc, Czech Republic) and indole-3-carbinol (I3C; Sigma-Aldrich, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2023Quote: ... The sections were then dehydrated in ascending concentrations of ethanol for 5 min each (2[×[35%, 50%, 70%, 80%, 90%, 3[×[100%) and washed 3 times for 5 min with propylene oxide (Sigma-Aldrich, #cat 110205-18L-C). Next ...
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bioRxiv - Cell Biology 2021Quote: ... P3 2M (AMS biotechnology #GSC-6201G 2M or #GSC-6101G 7M) and LIF ESGRO® Recombinant Mouse LIF Protein (1000 units/mL) (Millipore # ESG1107). For induction of the transgene Stemolecule Doxycycline hyclate 10 mg (Stemgent#04-0016 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pelleted cells were lysed and used to purify the recombinant proteins with glutathione–Sepharose 4B beads (Sigma Aldrich #GE17-0756-01). The purified protein or whole cell lysate was incubated with the respective agarose beads at various time points with continuous agitation and washed before imaging ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Biophysics 2020Quote: Both unlabeled and 15N labeled recombinant full-length αS was expressed and purified in Rosetta™ 2(DE3) pLysS (EMD Millipore®) cells using IMPACT™ system protocol (New England Biolabs® ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Cell Biology 2022Quote: Recombinant His6-EGFP used in the GFP intensity calibration (Figure 1B) was expressed in Rosetta 2 (DE3) competent cells (Millipore Sigma #71400) using pDual-EGFP plasmid (Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: TKO and QKO mESC were cultured in Serum/LIF media (DMEM-KO, 15% FBS, 1000 U/ml murine recombinant LIF (Millipore Sigma #ESG1106), 1× nonessential amino acids (100x ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant protein from the cell lysate was purified using Glutathione Sepharose® 4B GST affinity columns (Millpore Sigma, GE17-0756-05). The purified PCM1-GST fusion protein was analyzed on SDS-PAGE stained with Coomassie Blue (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Anti-His (SCBT, #sc-8036; used in 1:2000), anti-GST (SCBT, #459; used in 1:5000) and anti-Flag (Sigma-Aldrich, #F7425;used in 1:2000). Peroxidase conjugated anti-mouse (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto a nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2019Quote: ... The proteins of the eluate were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Microbiology 2020Quote: ... Smears were blocked in 3% bovine serum albumin (BSA; Sigma-aldrich). Slides were incubated with primary rat anti-HA (Roche ...
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bioRxiv - Biophysics 2020Quote: ... chips were incubated in 2% APTES (3-aminopropyl-triethoxysilane) (Sigma Aldrich) in anhydrous toluene (Alfa Aesar ...
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bioRxiv - Cancer Biology 2021Quote: ... Sesamol and 3′,4′-(Methylenedioxy) acetophenone were purchased from Sigma-Aldrich. All the analytical grade chemicals were procured from Merck Limited ...
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bioRxiv - Cell Biology 2020Quote: ... serum-deprived Calu-3 were loaded with 10μM DCF (Sigma D6883) in low-serum media for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... (3) LPS with rottlerin (dissolved in DMSO, Sigma Aldrich, Cat #: D4540) treatment (10 µg/mL LPS and 1 μM rottlerin ...
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bioRxiv - Cell Biology 2020Quote: ... GAPDH (glyceraldehyde-3-phosphate-dehydrogenase, Sigma Aldrich, G8795, dilution 1:7500), CV (ATP-Synthase ...
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bioRxiv - Cell Biology 2020Quote: MTT (3- [4,5-dimethyl-2-thiazolyl] −2,5-diphenyltetrazolium bromide, Sigma M5655) is a tetrazolium salt giving a yellow solution when diluted in the medium ...
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bioRxiv - Cell Biology 2020Quote: ... followed by treatment with 500 µM auxin (3-Indoleacetic acid; Sigma) for 5-6 hrs.
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bioRxiv - Cell Biology 2020Quote: ... and 0.5 mM of 3-isobutyl-1-methylxanthine (IBMX) (Sigma, I5879). After three days ...
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bioRxiv - Cell Biology 2020Quote: ... The surfaces were then incubated with 5% (3-Aminopropyl)trimethoxysilane (Sigma) in ethanol for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... concentrated using Centrifugal Filter Units (Amicon Ultra-15 3 kDa) (Millipore), and subjected to gel filtration chromatography using a Hiload 16/60 Superdex 75 gel filtration column (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (Sigma-Aldrich, 136149) in [0.1 M Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... for 3 hours and then treated with nigericin (20 μM; Sigma) for 45 minutes ...
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bioRxiv - Microbiology 2019Quote: ... B6 mice were injected intra-peritoneally with 3% peptone (Sigma-Aldrich) in saline ...
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bioRxiv - Developmental Biology 2021Quote: ... animals (3-5cm) were anesthetized in 0.01% p-amino benzocaine (Sigma). Spinal cord ablations were performed as previously described (Diaz Quiroz et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... and split every 2-3 days using accutase (Sigma-Aldrich, #A6964). For eGFP tagging of Brn2 and Ascl1 by CRISPR/Cas9 ...
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bioRxiv - Cell Biology 2022Quote: ... and 18 mm coverslips were activated using 3-aminopropyltrimethoxysilane (Sigma Aldrich) for 5 min and then for 30 min with 0.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... exogenous choline clearance in the presence of HC-3 (Sigma-Aldrich) was determined to test the relative contribution of heterozygous and homozygous CHT Val89 expression ...
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bioRxiv - Cell Biology 2022Quote: ... and 18 mm coverslips were activated using 3-aminopropyltrimethoxysilane (Sigma Aldrich) for 5 min and then for 30 min with 0.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Physiology 2022Quote: ... and primers (See Supplementary Table 3) were synthesized by Sigma-Aldrich. The expression of each gene was normalized to the geometric mean expression of two reference genes Hprt and Ubc ...
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bioRxiv - Neuroscience 2022Quote: ... in the presence of a glass bead (3 mm diameter, Sigma) in a final reaction volume of 200 μL ...
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bioRxiv - Neuroscience 2021Quote: ... anti-ataxin-3 (mouse monoclonal 1H9, MAB5360, 1:500-1000; Millipore), anti-MJD (rabbit polyclonal ...
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bioRxiv - Neuroscience 2021Quote: ... and 50 mM bovine serum albumin (BSA, A450-3, Sigma-Aldrich) in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 1mg/mL of Indole-3-acetic acid (Sigma, I3750-5G-A) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... Calu-3 cells (ATCC HTB-55) were cultured in MEM (Sigma) supplemented with 10% FBS ...