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Citations for Millipore Sigma :
5001 - 5050 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellet was washed with −20°C cold acetone (Sigma-Aldrich), vortexed ...
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bioRxiv - Cancer Biology 2023Quote: ... protein samples were prepared with sample buffer that contains 10% glycerol (Sigma) and 5% 2-mercaptoethanol (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... Protein concentration was measured using a Bradford assay (Sigma France, Lezennes, France). For SDS-PAGE analysis of purified proteins ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Millipore, IPVH00010). The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and the protein pellet resuspended and incubated in 2M hydroxylamine (NH2OH) (Sigma), pH 7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were incubated with 20 μl protein G-coated Sepharose (Sigma-Aldrich) previously conjugated with 5 μl of anti-FLAG antibody by overnight incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore) overnight at 4°C and blocked in either 5% nonfat powdered milk or 5% bovine serum albumin in TBS with Tween 20 (pH 7.6 ...
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bioRxiv - Plant Biology 2023Quote: ... Crude protein concentration was determined using the Bradford Reagent (Sigma-Aldrich, MO), following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was qualified by Bradford assays (Sigma-Aldrich, B6916-500 mL). 30 μg soluble proteins were resolved on a 15% sodium dodecyl sulphate poly acrylamide gel via electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... equal amounts of proteins were then incubated with anti-Flag Beads (Sigma), anti-Myc Beads (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Protein detection were performed with Immobilon Western Chemiluminescent HRP Substrate (ECL, Millipore). Band intensities were analyzed by ImageJ software.
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bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 25μg protein lysate were resuspended in Laemmli buffer 1X (Sigma, S3401-1VL), denatured at 95°C for 5 minutes and loaded onto a polyacrylamide gel (Mini-PROTEAN(R ...
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bioRxiv - Cell Biology 2023Quote: ... Separated proteins were then electroblotted onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA). Following the transfer ...
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bioRxiv - Microbiology 2023Quote: ... the protein-DNA complexes were immunoprecipitated with an anti-FLAG antibody (Sigma) and Protein A sepharose beads ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Millipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed protein solution was filtered (0.45 μm pore size filter, Millipore) and protein concentration was determined by absorbance measurements at 280 nm using extinction coefficient ε = 37752 M-1cm-1 ...
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed protein solution was filtered (0.45 μm pore size filter, Millipore) and protein concentration was determined by absorbance measurements at 277 nm using extinction coefficient ε = 5840 M-1cm-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualised using Immobilon Classico Western HRP substrate (Sigma-Aldrich, WBLUC0100). Primary antibody dilutions are as follow ...
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bioRxiv - Microbiology 2023Quote: ... Elution of bound proteins was performed with 500 µL FLAG-peptide (Sigma) solution (100 ng/µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were precipitated with 5x ice cold acetone (HPLC grade, Sigma-Aldrich) and centrifuged at 20,627 g for 10 min at 4° C ...
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bioRxiv - Immunology 2023Quote: ... Glycans were extracted with a polyvinylidene-fluoride protein-binding membrane (Merck Millipore). Digested glycans were analyzed by HILIC-UPLC as before (51 ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated to >20 mg/mL on a Centricon device (Millipore).
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bioRxiv - Biochemistry 2023Quote: SYPRO Orange Protein Gel Stain (Cat#S5692, Sigma-Aldrich, St. Louis, USA) were diluted 1:2500 into the assay buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... proteins entrapped in gel bands were reduced with 10 mM dithiothreitol (Sigma) at 60°C for one hour and then alkylated with 50 mM iodoacetamide (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was concentrated with a 100kDa-cutoff spin concentrator (Millipore), after which 1:10 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Protein synthesis was inhibited by adding cycloheximide (CHX) (Sigma, catalog number: 01810) at a final concentration of 100 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred on a MeOH-activated polyvinylidene fluoride (PVDF) membrane (Millipore) by semidry blotting for 1 h at 10V ...
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bioRxiv - Genetics 2022Quote: ... Eluted proteins were loaded on the centrifugal filter unit (Millipore-Sigma, UFC8030) and centrifuged at 3,500 g 4°C for 20 minutes to eliminate any remaining maltose from the elute ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentration was quantified by Coomassie staining using BSA standards (Sigma P0914).
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bioRxiv - Genomics 2023Quote: ... protein stability was assessed by adding 5 mg/ml cycloheximide (Sigma-Aldrich) to the cell growth medium for the indicated times prior to harvest ...
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bioRxiv - Genetics 2022Quote: ... Eluted proteins were loaded on the centrifugal filter unit (Millipore-Sigma, UFC8030) and centrifuged at 3,500 g 4°C for 20 minutes to eliminate any remaining maltose from the elute ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 μg/mL doxycycline hyclate (Sigma Aldrich), and the culture was placed in a shaking incubator maintaining 37°C and a 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was transferred to a low background fluorescence PVDF membrane (Millipore Sigma) and the membrane was blocked in 5% nonfat milk in 1x TBST ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred on an EtOH-activated polyvinylidene fluoride (PVDF) membrane (Millipore) by wet blotting overnight at 40 mA and 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were directly labeled using alkaline phosphatase-coupled streptavidin (Sigma-Aldrich). The blots were developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Cell Biology 2022Quote: ... The protein bands were visualized by an ECL detection system (EMD Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Whole-cell protein lysates were prepared in CelLytic M lysis buffer (Sigma) with 1% protease/phosphatase inhibitors (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... Cell lysates were incubated with Protein G sepharose beads (Sigma-Aldrich P3296) with anti-RpoC antibody (Biolegend clone NT73 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then filtered through a 10kDa protein filter (MRCPRT010, Sigma Aldrich) via centrifugation at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... The dialyzed protein was concentrated using a 30 kDa MWCO concentrator (Millipore) and was further purified by size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Genetics 2023Quote: ... the Cas9 protein was expressed in BL21 Rosetta Escherichia coli strain (Novagen) in magic medium at 37 °C for 12 h followed by 24 h at 18 °C ...
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bioRxiv - Cell Biology 2023Quote: ... protein inhibitor cocktail (1:100) and Benzonase nuclease (Novagen, 70746, 1:100) in PBS ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich). Prior to their use ...
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bioRxiv - Systems Biology 2023Quote: ... We then transferred proteins to a low background PVDF membrane (Millipore, IPFL00010) for 15h at 15V using the TRIS-Glycine buffer (48 mM Tris ...