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Citations for Millipore Sigma :
5001 - 5050 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the polyvinylidenedifluoride membranes were blocked for 1 h with 5% bovine serum albumin (BSA, Sigma-Aldrich), then incubated overnight at 4 °C with appropriate antibodies ...
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bioRxiv - Microbiology 2023Quote: ... the extracts were incubated with Marfey’s reagent (1-fluoro-2,4-dinitrophenyl-5-L-alanine amide; Sigma). After drying in a nitrogen stream ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 50 μL magnetic beads were incubated with 5 μg of Ago-1 antibody (Merck, Millipore, Germany) or IgG antibody (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 5 min in DB supplemented with 1 mg/mL of Soybean trypsin inhibitor (Sigma) and mechanically dissociated in DB supplemented with 0.6 mg/mL DNAse ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 μl of anti-rabbit 5 nm gold-labelled secondary antibody (1:10) (Sigma-Aldrich). The grid was washed ...
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bioRxiv - Microbiology 2023Quote: ... was layered over 200 µl of an oil mixture (1:5 mixture of mineral oil (Sigma) and di-n-butyl phthalate (BDH Ltd ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated with anti-FLAG M2 monoclonal antibody (Sigma-Aldrich, 1:2000 dilution in 5% milk) at 4 °C with rocking overnight ...
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bioRxiv - Microbiology 2023Quote: ... 5% w/v dry milk) and stained with polyclonal anti-FLAG M2 antibodies (1:10,000) (Sigma) and with polyclonal swine anti-mouse-HRP antibodies (1:1,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse monoclonal anti α-tubulin clone B-5-1-2 (Sigma-Aldrich, St. Louis, MO, USA) and anti-PABP [49] were used as loading controls ...
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bioRxiv - Biophysics 2024Quote: ... and anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma), revealed with donkey anti-mouse secondary antibodies conjugated to Alexa Fluor 555 ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 units/mL Pen/Strep antibiotic and Insulin 5 μg ml−1 (Sigma, Saint Louis, USA) at 37 °C in a balanced air humidified incubator with 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mM ATP and 1% (v/v) pyruvate kinase/lactic dehydrogenase enzyme mix (Sigma-Aldrich P0294) in 1X buffer (50mM K·HEPES pH 7.4 at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... Labelling was performed at RT for 1 h and quenched with 8 μl 5% hydroxylamine (Sigma). Labelled samples were acidified by adding 50 μl 1% TFA ...
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bioRxiv - Immunology 2024Quote: ... the incubated with secondary antibodies each diluted 1:200 in 5% normal equine serum (Sigma-Aldrich) in PBS for 2 hours ...
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bioRxiv - Immunology 2024Quote: Mice were injected once intraperitoneally with 1 mg of 5-bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich) and then fed with BrdU-containing water (0.8 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with 5 µg mL−1 propidium iodide (PI; Sigma-Aldrich, St. Louis, MO, USA) for 10 min at room temperature in the dark ...
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bioRxiv - Molecular Biology 2024Quote: ... the transgenic parasites were selected using negative selection by 5-FC (Sigma, USA; 1 mg/mL). Parasites were cloned by limiting dilution.
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bioRxiv - Neuroscience 2023Quote: ... Subsequent cytokine measurements interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were done using Milliplex magnetic bead panel (Millipore Sigma, MA, US) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The loading reaction (480 µL) was spun in a pre-rinsed Amicon Ultra 0.5 mL Centrifugal Filter with 100 kDa MWCO (Merck Millipore, 6 000 g, 6 min). After two washes with water (480 µL ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of purified LDL were mixed with 50 µL DiI stock solution (3 mg/mL in DMSO; Sigma) and incubated at 37℃ for 8 h ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by selection in 1 μg/mL (C4-2B) or 1.5 μg/mL (DU145, PC-3) of puromycin (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... Then mice were randomly infused 10 minutes before the test with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed with PBS-T 3 times before the plates were incubated with 75μl Streptavidin peroxidase (1:1000, Sigma) for 60 minutes ...
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bioRxiv - Plant Biology 2019Quote: Treatments with the NO-scavenger 2-(4-Carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazol-1-yloxy-3-oxide potassium salt (c-PTIO salt, Sigma Aldrich, Darmstadt, Germany) were performed 1 hour prior to ethylene treatments to allow for treatment combinations ...
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bioRxiv - Microbiology 2019Quote: ... The spheroplasts were then washed 3 times in SPM buffer permeabilized by incubation in 1 mL of 0.1% triton X-100 (Sigma) in PBS for 2 minutes on ice and washed twice in PBS ...
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bioRxiv - Plant Biology 2019Quote: ... 150 mM NaCl and 10 mM HEPES pH 7.4 and eluted with 100 μg ml−1 3×FLAG peptide (Sigma).
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bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were cleaved off the column with thrombin diluted 1:250 in thrombin cleavage buffer (Novagen #69671-3). The thrombin solution was loaded manually into the column with a syringe ...
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
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bioRxiv - Plant Biology 2020Quote: ... epitopes were blocked with 0.1 M PBS / 1% Cold Water Fish Skin Gelatin / 0.2% BSA with 3% sucrose (all chemicals from Sigma Aldrich), incubated with the primary antibody anti-GFP (1:250 ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM sodium phosphate pH 8.0, 3% glycerol, 1% Triton X-100, 15 mM imidazole, and 1x protease inhibitor [Roche, Sigma]) and sonicated ...
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bioRxiv - Cell Biology 2021Quote: ... cells were trypsinized than fixed with PBS/ formalin 3% then incubated with PBS/ Triton 0.1%/ TRITC-phalloïdin 1 µg/mL (Sigma Aldrich) during 5 min ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then washed twice with PBS prior to 20 min permeabilization with 0.5% Triton X-100 and 1 h blocking with 3% BSA (Sigma) at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... before 3 washes with 1 mL PBS and saturation with a 0.5% BSA solution in PBS 1X (Sigma A7888) for 15 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... The cultures were then blocked for 1 hour at room temperature with 3% (w/v) BSA in PBS (Sigma), followed by incubation with anti-Gfap (1:100 ...
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bioRxiv - Cell Biology 2021Quote: Chromatin was isolated from supernatant by adding 3 volumes of TE with 1% SDS and RNAse A (Sigma R6513), and vortexing to mix ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK 293T cells were transfected with a 1:3 ratio of DNA:polyethylenimine (PEI, 25 kDa linear, Sigma-Aldrich). Transfection of siRNA was performed using X-tremeGENE siRNA Transfection Reagent (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Biochemistry 2022Quote: ... Cholesteryl hemisuccinate (CHS, C6013) and 1-stearoyl-2- hydroxy-sn-glycero-3-phosphocholine (Lyso-PC) were purchased from Sigma. Brain phosphatidylinositol-4-phosphate (PI(4)P) ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at RT for 1 hour in a blocking solution containing 3% Donkey Serum (Biowest) and 0.3% Triton X-100 (Sigma) in PBS 0.01M ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of medium containing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (1 mg/mL) (Sigma-Aldrich) was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: Human melanoma cells were seeded in 6-well plates with 0.1-1 mL lentivirus in a final volume of 3 mL with 4 µg/mL polybrene (Millipore). Cells were spun at 1,000 x g for 2 h at 30 ºC ...
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bioRxiv - Immunology 2021Quote: ... at room temperature for 3 hours and followed by streptavidin-HRP (dilution 1:1000, Sigma-Aldrich, Cat# 18-152) for 45 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... frozen tissue (from 2–3 mice or 1 rat) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Band intensity was measured as the sum optical density by using ImageJ software (version1.4; NIH) and normalized to control labelling of glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:5000, Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... 1-3 million cells were treated for 24 hours with 1.2 uM aphidicolin in PBS (Millipore Sigma cat#: A0781). Cells were attached to Concanavalin A coated slides for one hour at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... The primary antibody (1:100 Anti-Tau [3-repeat isoform RD3] Antibody, clone 8E6/C11, 05-803, Sigma-Aldrich and 1:100 Anti-Tau [4-repeat isoform RD4] Antibody ...