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Citations for Millipore Sigma :
4951 - 5000 of 10000+ citations for Integrin alpha 4 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... solution followed by formaldehyde solution 4% (Sigma-Aldrich, F8775). 50-100 µm slices were blocked using a PBS solution containing 5% normal donkey serum and 0.4% Triton x-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... using Amicon Ultra-4 filter units (Millipore, Burlington, MA) with a 100 kDa MWCO membrane ...
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bioRxiv - Neuroscience 2024Quote: ... in combination with 4 μM rotenone (Sigma-Aldrich, 557368). OCR were measured by Seahorse XFe96 Flux Analyzer using a mix (30 s ...
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bioRxiv - Bioengineering 2024Quote: ... 2 × 10−4 M L-cystine (C7602, Sigma-Aldrich), 100 μg/mL sodium pyruvate (11360070 ...
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bioRxiv - Biophysics 2024Quote: ... and 2.5% BSA (Merck Millipore, Probumin #81-066-4). Primary antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2024Quote: ... progesterone (P4, 4 µg/mL, Sigma catalog #P8783-5G), human chorionic gonadotropin (hCG ...
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bioRxiv - Developmental Biology 2024Quote: ... and 100 ppm 4-hydroxy TEMPO (4HT, Sigma, 176141)] on a chamber composed of a Gel Slick-treated glass slide and 1-layer Scotch Magic Tape spacers (∼56 µm ...
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bioRxiv - Biochemistry 2024Quote: ... and 4-methylumbelliferone-N-acetyl-neuraminic acid (Sigma-Aldrich) at a final concentration of 250 µM in 25 mM sodium acetate buffer ...
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bioRxiv - Microbiology 2024Quote: ... Sporozoites were fixed using 4% paraformaldehyde (Sigma‒Aldrich, HT5012), permeabilized with 0.1% Triton-X-100 (Sigma‒Aldrich ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... at pH 4 or 5.4 or with MOPS (Sigma) at pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated in 4% w/v BSA (Sigma-Aldrich, A2153)/TN buffer for 1-2 hours at room temperature in a humid box.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated for 4 hours with Resazurin (Sigma) and plates were read using an Infinite M1000 pro plate reader (TECAN) ...
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bioRxiv - Molecular Biology 2024Quote: ... Glutaryl-Ala-Ala-Phe-4-methoxy-β-naphthylamide (Sigma). For the basal peptidase rate ...
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bioRxiv - Systems Biology 2024Quote: ... in situ fixated in 4% PFA (Sigma Aldrich, USA) at RT and washed in 50mL 1x PBS (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Microbiology 2020Quote: ... were immunized intraperitoneally with 17 μg of recombinant NRP1 b1b2 mixed with an equal volume of complete Freund’s adjuvant (Sigma–Aldrich Chemie, Steinheim, Germany), followed by a booster immunization four weeks later of the same dose mixed with incomplete Freund’s adjuvant (Sigma–Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant expression constructs were transfected into CHO Tet-On cells and expression was carried out in the presence of biotin as previously described.18 Culture medium containing secreted recombinant A1 or A1A2A3 protein was collected and concentrated five times using an Amicon Ultra-15 Centrifugal Filters (EMD Millipore, Billerica, MA). Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare ...
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bioRxiv - Genomics 2022Quote: ... in a 12-well cell culture plate coated with Human recombinant laminin 511 (BioLamina, Cat. LN511-0202) and fixed in 1xPBS with 3.7% formaldehyde (Millipore Sigma, Cat. F8775-25ML) for 10 mins and then permeabilized in ice-cold 70% (vol./vol. ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Bioengineering 2023Quote: ... ethylenediaminetetraacetic acid (EDTA), iodoacetamide (IAM), urea, recombinant porcine trypsin, K2IrCl6, and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO). L-histidine monohydrochloride monohydrate and L-histidine were purchased from Avantor (Allentown ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... Cells that received Interferon-β pre-treatment were stimulated for 18 hours with 100 units of recombinant human Interferon-β protein (Millipore, IF014) prior to infection.
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bioRxiv - Cell Biology 2023Quote: ... East Brunswick, NJ, USA), human recombinant Stem Cell Factor (100ng/mL, supernatant SCF producing cell line) and dexamethasone (1μM; Sigma, St. Louis, MO, USA).5 EBL cultures were kept at a density of 0.7-1.5 million/mL by dilution with fresh medium ...
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bioRxiv - Microbiology 2024Quote: ... for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for QD8 production ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EDTA with freshly added 1 mM DTT), then concentrated to 500 μl using Amicon Ultra-4 (4 mL, 3 kDa cut-off) (Millipore, Darmstadt, Germany, UFC800324) and loaded onto a Superdex75 10/300 GL size exclusion column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with surface markers for 30 min at 4°C followed by fixation with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO) at 4°C for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed 3 times over 45 mins before fixation in phosphate-buffered saline (PBS) containing 4 % paraformaldehyde and 4 % sucrose (PFA, Sigma-Aldrich, Cat.# P6148) at 4 °C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... N- (Piperidin-1-yl)-5- (4-iodophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 3 mg/kg; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Neuroscience 2021Quote: ... nonspecific staining was blocked with blocking solution (TBST and 4% normal donkey or normal goat serum) and incubated overnight at 4°C with SOX9 antibody (1:2000, Millipore, AB5535, RRID: AB_2239761). Sections were washed and incubated with Alexa 568-conjugated secondary antibody (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Mice received an injection of leucine into the NTS in their home cage as above and 80 minutes later were dosed with the tamoxifen metabolite 4-hydroxytamoxifen (4-PHT, Sigma, 40mg/kg ip) prepared using a formulation described previously (Ye et al. ...
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bioRxiv - Immunology 2020Quote: ... CVLs were centrifuged at 1,800rcf for 1 hour at 4°C then cleared using Spin-X® Tubes centrifuged at 16,000rcf for 30min at 4°C (Sigma-Aldrich, Lyon, France), aliquoted ...
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bioRxiv - Cancer Biology 2021Quote: We purchased six-week-old C57BL/6 female mice and used a reported chemical carcinogen 4-nitroquinoline 1-oxide (4-NQO, cat#N8141, Sigma, St Louis, MO) to induce ESCC as previously reported [38] ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilized cells were blocked with 5% BSA diluted in PBS for 4 h at 4°C and incubated overnight with pan-Cadherin antibody (Sigma, C1821, 1:500), MRP2 antibody (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations were performed by incubating cleared lysates at 4°C for 4 hours using 15 μl of FLAG M2 Affinity Gel (Sigma-Aldrich - catalog #F2426) with gentle mixing ...
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bioRxiv - Microbiology 2021Quote: ... A total of 4 mL of the wash was extracted and placed in a Amicon® Ultra-4 Ultracel®-50k (Merck Millipore Ltd.) filter tube and centrifuged for 7 minutes at 7000 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... x Talin1fl/fl (females) intercross received three consequent daily intraperitoneal injections of 4-hydroxy-tamoxifen (4-OHT, purity ≥98% Z isomer, Sigma-Aldrich Cat# H7904) (80 μg/pup diluted in ethanol/peanut oil ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were post fixed in 4 % PFA over night at 4°C and cryoprotected in 30 % sucrose (w/v, Sigma Aldrich, Taufkirchen, Germany). Following cryoprotection ...
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bioRxiv - Neuroscience 2024Quote: Population tagging was performed via a single intraperitoneal (i.p.) injection of either 0.9% saline or 40 mg/kg 4-hydroxytamoxifen (4-OHT; Sigma, St. Louis, MO, Cat# H627) immediately following sociability testing in the presence of predator odor on PN16-17 83–85 ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected brains were post-fixed for 2 h at 4°C in 4% PFA and cryoprotected in successive solutions of 15% and 30% sucrose (Sigma-Aldrich, Cat# S0389) in PBS ...
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bioRxiv - Pathology 2023Quote: ... 1-6 months-old adult mice were shaved on the dorsal skin and treated topically with 40 μl of 1-5mg/ml 4-hydroxytamoxifen (4-OHT; Sigma, St. Louis, MO) in ethanol every other day for 4 times ...
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bioRxiv - Microbiology 2024Quote: ... P3HR-1 ZHT/RHT or Akata cells lytic induction was triggered by addition of 400 nM 4-hydroxy tamoxifen (4-HT) (Sigma #68392-35-8) for 24 h or by 15 µg/ml α-human IgG (Agilent #A042301-2 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples (5 μg) were used to determine GCase activity upon addition of 10 mM 4-Methylumbelliferyl ß-glucophyranoside (4-MU, Sigma-Aldrich, # M3633) in 1% BSA with or without 2 mM conduritol-b-epoxide (CBE ...
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bioRxiv - Developmental Biology 2023Quote: Vital dye staining of lateral line hair cells of the 4 dpf embryos was performed using DASPEI (2-(4-(dimethylamino) styryl)-N-ethylpyridinium iodide) (Sigma-Aldrich, USA, D0815), 0.8 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: To generate ApcKO tumoroids, Lgr5-2a-CreERT2::Apcflox/flox healthy organoids were treated with 4-hydroxytamoxifen (4-OHT, 500nM) (Sigma cat#SML1666-1ML) for 24hrs - intestinal stem cells with successful Cre-recombination activated tdTomato expression and appeared red under a fluorescence microscope ...
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bioRxiv - Plant Biology 2021Quote: ... PVDF membranes were blocked with 5% milk followed by an incubation with first antibodies for REM1.2/1.3 followed by 3 washes and an incubation with second α-rabbit antibody fused to HRP against rabbit (Sigma). Clarity Western 701 ECL (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was washed twice more before incubating with the secondary antibody (Anti-goat IgG raised in rabbits for the Mu and Anti-rabbit IgG raised in goats for the Sigma) for 1-2 hours at a 1:30000 dilution in blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were immunodetected using 1:2500 dilution of the rabbit anti-TbKH polyclonal antibody and 1:15,000 dilution of goat anti-rabbit-HRP antibody (Sigma-Aldrich). The chemiluminescent protocol was used for developing as indicated below.