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Citations for Millipore Sigma :
451 - 500 of 7556 citations for ssc mir 136 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... DuolinkTM In Situ PLA Mouse/Rabbit kit with red detection reagents (Sigma-Aldrich, DUO92101-1KT) was used according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Apoptosis was determined with the ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Sigma, S7110) (2017-2019) ...
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bioRxiv - Pathology 2021Quote: ... OxyIHC staining was performed according the Millipore OxyIHCTM oxidative stress detection kit protocol (Millipore, S7450).
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bioRxiv - Genetics 2019Quote: ... TUNEL assays were performed using an ApopTag Peroxidase In Situ Apoptosis Detection Kit (Millipore, S7100). Thymus sections were used as positive controls ...
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bioRxiv - Systems Biology 2021Quote: ... Apoptosis was assessed in dissociated spheroids using Annexin V-FITC Apoptosis Detection Kit (Sigma-Aldrich) according the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Dying cells were visualized with the ApopTag® Red In Situ Apoptosis Detection kit (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... TUNEL staining was performed using In Situ Cell Death Detection Fluorecein kit (Sigma cat# 11684795910) prior to addition of anti-recoverin primary antibody ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: PLA was performed using the Duolink Detection Reagents Brightfield Kit (Sigma-Aldrich, Cat. No. DUO92012) and associated wash buffers (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was recovered and used with the protease fluorescent detection kit (PF0100 – Sigma-Aldrich). The protocol was adjusted from the manufacturers’ instructions in the following ways ...
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bioRxiv - Neuroscience 2023Quote: ... PLA signals were detected using the red Duolink in situ detection reagent kit (DUO92008, Sigma) and imaged on the Zeiss LSM700 ...
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bioRxiv - Synthetic Biology 2023Quote: ... This assay was performed according to BACE1 Activity Detection Kit protocols (Sigma, St. Louis, MO). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: PLA was performed using the Duolink in situ detection reagents far-red kit (Sigma, DUO92013), PLA-probe anti-mouse PLUS (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... DuolinkTM In Situ PLA Mouse/Rabbit kit with red detection reagents (Sigma-Aldrich, DUO92101-1KT) was used according to manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore; Watford, UK). The following antibodies were used ...
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bioRxiv - Immunology 2023Quote: ... they were detected for intermolecular binding using Duolink flow PLA detection kit (Green) (Sigma/Merck) with a reaction time of 100 minutes for post-mouse/rabbit probe linking amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescent signal was generated using Duolink In Situ Detection Reagents Red kit (Sigma-Aldrich, DUO92008). Cells were mounted and the nuclei were stained using the Duolink In Situ Mounting Medium with DAPI (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... an in situ detection kit was used according to the manufacturer’s protocol (Sigma-Aldrich, USA). Sample pretreatment with DNase I served as positive control and TUNEL reaction mixture without terminal transferase (TdT ...
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bioRxiv - Bioengineering 2023Quote: ... ApopTag® Peroxidase In Situ Apoptosis Detection Kit was purchased from Millipore (Billerica, MA, USA).
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bioRxiv - Neuroscience 2024Quote: ... BACE1 activity was measured using the β-Secretase (BACE1) Activity Detection Kit (Fluorescent) (Sigma: CS0010). Equal amounts of lysate were added to each well and run in duplicate ...
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bioRxiv - Cancer Biology 2023Quote: ... ECL protein detection (Millipore) was performed with a Fusion Fx7 chemiluminescent imager ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich, USA) followed by cloning of the same using pGEM-T Easy Vector System I ...
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bioRxiv - Developmental Biology 2022Quote: ... Several probes were produced by PCR amplification (KAPA2G Fast ReadyMix PCR kit, Sigma Israel, KK5101). cDNA templates were synthesized by RNA precipitation followed by reverse transcription PCR ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... PCR products were purified with the High Pure PCR Product Purification Kit (Sigma Aldrich, 11732676001) and cloned into the pcDNA3.1 Hygro+ mammalian expression vector (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Triplicate reactions were started at RT in PCR tubes by addition of an NAD+-mixture resulting to final concentrations of 24.5 µM NAD+ (Sigma, N3014) and 0.5 µM of biotinylated NAD+ (Trevigen ...
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bioRxiv - Molecular Biology 2023Quote: 10% of the RT reaction was used as the template in 25 μl PCR reactions containing 1.25U of JumpStart Taq Polymerase (Sigma D9307), 1×PCR buffer (Sigma P2192) ...
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bioRxiv - Developmental Biology 2022Quote: ... A probe for Nr2f2 was prepared by amplifying a cDNA fragment by RT-PCR (primers provided below, Table 4) from total RNA isolated from E9.5 embryos with Tri Reagent (Sigma #93289) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA of silkworm ovaries and cloned into pIEx-1 vector (Merck Millipore/Novagen) or pIExZ vector by In-fusion cloning kit (Takara).
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed with SYBR Green Quantitative RT-qPCR Kit (Sigma-Aldrich; Cat. No. QR0100) using GAPDH as a housekeeping gene ...
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bioRxiv - Molecular Biology 2023Quote: ... or MMLV-RT (Sigma), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Ant-miR-218 or Ant-scrambled was dissolved in sterile PBS (Sigma-Aldrich, Oakville, ON, Canada) at a final concentration of 0.3nM and infused with a total volume of 0.5μl of Ant-miR-218 or Ant-scrambled over a 7-min period as in(31 ...
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bioRxiv - Neuroscience 2019Quote: ... Ant-miR-218 or Ant-scrambled were dissolved in sterile PBS (Sigma-Aldrich, Oakville, ON, Canada) at a final concentration of 0.3 mM ...
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bioRxiv - Developmental Biology 2022Quote: ... The dehydrated samples were then incubated in a 1:1 mixture of propylene oxide and absolute ethanol for five minutes at RT followed by a two times 10 minutes incubation in pure propylene oxide (Sigma-Aldrich, Merck, Darmstadt, Germany), and a 30 minutes incubation in a 1:1 mixture of propylene oxide and Spurr resin (Low Viscosity Spurr Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 mMTris-HCl in 2x SSC) supplemented with 1 μl of 3.7 mg/ml glucose oxidase (Sigma-Aldrich) and 1 μl of 5 mg/ml Catalase (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were incubated for pre-hybridization in wash buffer (2x saline-sodium citrate (SSC) with deionized formamide (Sigma) 10% ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in hybridization buffer (50% Formamide, 4x SSC pH 4.5, 1% SDS, 50μg/ml heparin (Sigma), 10μg/ml tRNA from baker’s yeast (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... Coverslips were incubated for pre-hybridization in wash buffer (2x saline-sodium citrate (SSC) with deionized formamide (Sigma) 10% ...
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bioRxiv - Genomics 2019Quote: ... The supernatant was discarded and the pellet was resuspended in a small volume of SSC cocktail (3xSSC [Sigma; S0902] ...
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bioRxiv - Cell Biology 2022Quote: ... dishes were washed with wash buffer (1x saline-sodium citrate, SSC, Sigma-Aldrich, in 10% deionized formamide, Ambion) for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the slides were washed with the RNAscope wash buffer and incubated with 5X SSC (Sigma-Aldrich, S6639-1L) overnight at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... SSCs were seeded on STO feeders which were prepared 1 day before on Millicell EZ SLIDE (PEZGS0816, Millipore). SSCs were treated with 4-OHT at 0.1µM or RA at 1 µM or vehicle for the indicated time ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were washed with stringent buffer (1:1, formamide:4X SSC buffer; Thermo Fisher #AM9342; Sigma-Aldrich #S6639) at 37°C twice for 25 minutes each ...
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bioRxiv - Microbiology 2020Quote: ... Amplicons were purified using a PCR cleanup kit (Sigma) and quantified using a Qubit 2.0 fluorometer (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... using RedExtract-n-Amp tissue PCR kit (Sigma-Aldrich). The G2 progeny of genome-edited males were then raised until homozygous stocks were established ...
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bioRxiv - Bioengineering 2020Quote: ... Apoptosis was assessed by DNA fragmentation staining using a commercially available kit (In Situ Cell Death Detection Kit, TMR red, Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Cell proliferation was determined with the iClick EdU Imaging Kit (ABP Biosciences) and apoptosis was measured using the ApopTag Peroxidase in Situ Apoptosis Detection Kit (Millipore Sigma), following the manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... Polymerase chain reaction (PCR) samples were prepared using KAPA2G Fast PCR Kit (Sigma St. Louis, MO) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was purified using the GenElute PCR purification kit (Sigma-Aldrich, St. Louis, MO). The indexing PCR was carried out to attach the dual indices (Appendix D6.2 ...
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bioRxiv - Developmental Biology 2021Quote: ... All PCR reactions were column purified (High Pure PCR Product Purification kit, cat#11732668001, Sigma Aldrich). 2 ng/μl of purified PCR products were injected into either N2 or SM507 strains along with 100 ng/μl of pRF4 plasmid as a selection marker to create transgenic lines with extrachromosomal arrays.