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Citations for Millipore Sigma :
451 - 500 of 9836 citations for Recombinant Human CD3D Flag and Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...
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bioRxiv - Genetics 2019Quote: ... 10% FCS (Sigma) supplemented with FLT3L (50 ng/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 % FCS (Sigma) and P/S (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... + 10% FCS (Sigma,) + 1 x (final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... 10% FCS (Sigma), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FCS (Sigma), NEAA ...
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bioRxiv - Biochemistry 2023Quote: ... 5 % FCS (Sigma) in DPBS for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant MEK and recombinant ERK were purchased from Sigma. Indicated amounts of recombinant GST-HSF-1 (0.1–0.4 mg ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed with PBST and incubated with a goat anti-human Fc−peroxidase antibody diluted 1:5,000 (Sigma) in blocking buffer for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were then washed and incubated with a 1:5,000 dilution of peroxidase-conjugated anti-human Fc antibody (Sigma) for 1 hr ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were blocked for 1 h in AD before addition of assay samples for 1 h followed by incubation with 1/20,000 dilution Goat anti-human Fc-HRP (Sigma). All steps were conducted at 37°C and separated by three washes with PBS-T (0.05% Tween-20) ...
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bioRxiv - Biochemistry 2021Quote: ... and purified using DDDDK-tagged protein purification gel (MBL, Japan) followed by elution with 0.1 mg/ml FLAG Peptide (Sigma-Aldrich Co., St. Louis, Missouri, the US). To remove the flag peptide ...
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bioRxiv - Microbiology 2022Quote: ... FLAG (monoclonal mouse anti-FLAG M2 antibody, Sigma), and GAPDH antibody GA1R (Covalab ...
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bioRxiv - Cell Biology 2021Quote: FLAG - FLAG tag antibody [M2] (Sigma, F-1804)
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bioRxiv - Genetics 2019Quote: ... and Flag (anti-Flag, M2, 1:500, Sigma). FITC- conjugated goat anti-phalloidin ...
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bioRxiv - Microbiology 2023Quote: ... or FLAG antibody (Sigma Monoclonal FLAG antibody M2) in TBST with 5% NFDM (w/v) ...
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bioRxiv - Molecular Biology 2020Quote: ... Flag (Sigma), HA (Sigma) ...
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bioRxiv - Genetics 2020Quote: ... FLAG (Sigma), myc ...
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bioRxiv - Cancer Biology 2020Quote: ... FLAG (Sigma; #SAB4200071 ...
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bioRxiv - Cancer Biology 2021Quote: ... FLAG (Sigma), CDC20 (Santa Cruz) ...
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bioRxiv - Cell Biology 2019Quote: ... flag (Sigma); anti-IL6 ...
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bioRxiv - Cell Biology 2019Quote: ... Flag (Sigma), hydroxyproline (Abcam) ...
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bioRxiv - Molecular Biology 2021Quote: ... FLAG (Sigma monoclonal antibody catalogue number F1804) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein on the membrane were probed with antibodies (either anti-HA (Roche),-FLAG (Sigma), or -p23 (SCBT) ...
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bioRxiv - Genetics 2024Quote: ... Flag (Sigma F1804 ...
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bioRxiv - Genomics 2022Quote: ... FITC-tagged NeuN antibody (Sigma, MAB377) was prepared following manufacturer’s instructions (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Plates were washed with TBS containing 0.2% Tween and incubated for 1 hour at 37°C with Goat anti-human Fc (Sigma, A9544) or Fab (Jackson immunoResearch AB_2337617 ...
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bioRxiv - Cell Biology 2021Quote: ... plates were washed 4 times with PBST and further incubated with 100 μL per well of goat anti-human IgG (Fc specific)-Peroxidase antibody (1: 5000 dilution, Sigma) for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: rAkata EBV-infected cells was reactivated at 1×106 cells/mL with a goat polyclonal anti-human IgG Fc-specific antibody (Sigma) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was washed 5 times with PBS and incubated for 1 hour with goat anti-human IgG (Fc specific) –peroxidase (#A0170, Sigma). Binding signals were developed and stopped by the addition of TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Cell Biology 2019Quote: ... Flag beads (Anti Flag M2 Affinity Gel, Sigma A2250) were added to the cell extract and the mixture incubated for 2 hrs at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... and antibodies against FLAG (Sigma-Aldrich, anti-FLAG-M2), TCAB1 (Proteintech ...
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bioRxiv - Molecular Biology 2021Quote: ... 1mg of chromatin for Flag-ChIP (Flag-M2-Sigma) or anti-IgG antibody (Millipore- PP64) ...
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bioRxiv - Molecular Biology 2020Quote: ... Set2-FLAG ChIP used Anti-FLAG M2: (Sigma F1804) and monitored Set2 association with act1+ or clr4+ loci as a ratio of signal from input DNA ...
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bioRxiv - Microbiology 2023Quote: ... monoclonal mouse α-Flag (Sigma-Aldrich 41106000 M2-Flag), α-T3D virion antisera ...
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bioRxiv - Developmental Biology 2023Quote: ... and a FLAG antibody (anti-Flag-HRP, Sigma-Aldrich). For interaction studies of biotinylated AtRALF4/19 with GST-tagged ZmFERL7/9 and MBP-tagged ZmFERL1/4 ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
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bioRxiv - Cell Biology 2019Quote: ... containing 10% FCS (Sigma), 100U/ml penicillin and 100µg/ml streptomycin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS (Sigma-Aldrich), 1% Penicillin-Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented 10% FCS (Sigma), 1% Pen Strep (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... 10% FCS (Sigma Aldrich), 100 units/ml penicillin ...